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The map label for this gene is gidB

Identifier: 86608642

GI number: 86608642

Start: 1204260

End: 1205042

Strand: Reverse

Name: gidB

Synonym: CYB_1166

Alternate gene names: 86608642

Gene position: 1205042-1204260 (Counterclockwise)

Preceding gene: 86608643

Following gene: 86608641

Centisome position: 39.55

GC content: 62.07

Gene sequence:

>783_bases
ATGAAGAAGGCTGACGCTCCTTCCTCTGAGCCTCCAGAGCTCAAGGCCCTGTGGGAAAGCTGGCTGGCGGATCTGGGTTG
GCAGCCTTCTGAACAGCAGCAGCAACAACTGCAACAGCTCTATCCGCTGGTGATGGCCGGAAACCGAACCCAGAACCTGA
CCCGCATCACCGATCCCATCGACTTTTGGGAGAAACACCTGTGGGATTCGCTGCGGGGCCTACGCCTGCTGGGATCCTGG
GAGGCAATCCAAGCTCAGCCCTGGCGGGGGATCGACATCGGCACCGGGGCAGGGTTTCCAGGGATCCCGGCCCAAATTGC
CCTGCCCCAAACCCGCTTTACCCTGCTGGACAGCACCCAACGCAAGATTGCCTTTGTGCAGGACATCTTGCAAAGGCTCT
CCCTCACCCAAGCGCGGGCAGTGGCTCAGCGGGCCGAAATTTGGGGGCAAGACCCGCAGGAACGGGGCAGCTATGATATT
GCTCTGGCCAGGGCCCTGGCCTCGGCGGAGATCTGCGCCGAGTATTGTCTGCCACTGCTCAAGGTGGGGGGACGCGCCAT
CCTCTATCGCGGCCATTGGACCGAGGCAGAAGCCCAAACTTTGGAGCAGGCCCTTGAGCTCCTGGGCGGAAAACTGATCC
ATGTAGAAGCCTTCACCACACCCCGCAGCCGTGGGGTGCGCCACTGCCTACTGCTGGAGAAAGTAGCCCCCACGCCGCCT
CCTTATCCCCGCTCTCCCGGCACTCCCAAACGCCAACCGTTGGGCCAAAGCAATCGCCCATGA

Upstream 100 bases:

>100_bases
AGCTGCTGGGCACCGACCGTTCTTTGCAGGGCTTGACCCAAGAGCTGAAAGGCTGGGCCGACAGAGCCATCCCCGCTGGA
TTGAAACTGGGATCCCTGGG

Downstream 100 bases:

>100_bases
AGGTTAGGATCCTGAGTTTCCATCTTGCTACAAAAGCTTCTCCCAATTCTCGGACAAAAAGCTGGAAGGATGGCTTTGCA
AATGCCATTGGGAAGACTAA

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MKKADAPSSEPPELKALWESWLADLGWQPSEQQQQQLQQLYPLVMAGNRTQNLTRITDPIDFWEKHLWDSLRGLRLLGSW
EAIQAQPWRGIDIGTGAGFPGIPAQIALPQTRFTLLDSTQRKIAFVQDILQRLSLTQARAVAQRAEIWGQDPQERGSYDI
ALARALASAEICAEYCLPLLKVGGRAILYRGHWTEAEAQTLEQALELLGGKLIHVEAFTTPRSRGVRHCLLLEKVAPTPP
PYPRSPGTPKRQPLGQSNRP

Sequences:

>Translated_260_residues
MKKADAPSSEPPELKALWESWLADLGWQPSEQQQQQLQQLYPLVMAGNRTQNLTRITDPIDFWEKHLWDSLRGLRLLGSW
EAIQAQPWRGIDIGTGAGFPGIPAQIALPQTRFTLLDSTQRKIAFVQDILQRLSLTQARAVAQRAEIWGQDPQERGSYDI
ALARALASAEICAEYCLPLLKVGGRAILYRGHWTEAEAQTLEQALELLGGKLIHVEAFTTPRSRGVRHCLLLEKVAPTPP
PYPRSPGTPKRQPLGQSNRP
>Mature_260_residues
MKKADAPSSEPPELKALWESWLADLGWQPSEQQQQQLQQLYPLVMAGNRTQNLTRITDPIDFWEKHLWDSLRGLRLLGSW
EAIQAQPWRGIDIGTGAGFPGIPAQIALPQTRFTLLDSTQRKIAFVQDILQRLSLTQARAVAQRAEIWGQDPQERGSYDI
ALARALASAEICAEYCLPLLKVGGRAILYRGHWTEAEAQTLEQALELLGGKLIHVEAFTTPRSRGVRHCLLLEKVAPTPP
PYPRSPGTPKRQPLGQSNRP

Specific function: Specifically methylates the N7 position of a guanosine in 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=212, Percent_Identity=28.7735849056604, Blast_Score=87, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_SYNJB (Q2JMA3)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477404.1
- ProteinModelPortal:   Q2JMA3
- SMR:   Q2JMA3
- STRING:   Q2JMA3
- GeneID:   3900694
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_1166
- TIGR:   CYB_1166
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HLWDSLR
- PhylomeDB:   Q2JMA3
- ProtClustDB:   PRK00107
- BioCyc:   SSP321332:CYB_1166-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 29091; Mature: 29091

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKADAPSSEPPELKALWESWLADLGWQPSEQQQQQLQQLYPLVMAGNRTQNLTRITDPI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCHH
DFWEKHLWDSLRGLRLLGSWEAIQAQPWRGIDIGTGAGFPGIPAQIALPQTRFTLLDSTQ
HHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHH
RKIAFVQDILQRLSLTQARAVAQRAEIWGQDPQERGSYDIALARALASAEICAEYCLPLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KVGGRAILYRGHWTEAEAQTLEQALELLGGKLIHVEAFTTPRSRGVRHCLLLEKVAPTPP
HHCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCC
PYPRSPGTPKRQPLGQSNRP
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKADAPSSEPPELKALWESWLADLGWQPSEQQQQQLQQLYPLVMAGNRTQNLTRITDPI
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHCCHH
DFWEKHLWDSLRGLRLLGSWEAIQAQPWRGIDIGTGAGFPGIPAQIALPQTRFTLLDSTQ
HHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHH
RKIAFVQDILQRLSLTQARAVAQRAEIWGQDPQERGSYDIALARALASAEICAEYCLPLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
KVGGRAILYRGHWTEAEAQTLEQALELLGGKLIHVEAFTTPRSRGVRHCLLLEKVAPTPP
HHCCEEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCC
PYPRSPGTPKRQPLGQSNRP
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA