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The map label for this gene is fabI [H]

Identifier: 86608636

GI number: 86608636

Start: 1198195

End: 1198971

Strand: Reverse

Name: fabI [H]

Synonym: CYB_1160

Alternate gene names: 86608636

Gene position: 1198971-1198195 (Counterclockwise)

Preceding gene: 86608637

Following gene: 86608635

Centisome position: 39.35

GC content: 57.66

Gene sequence:

>777_bases
ATGCTACAGCTGACGGGAAAGAAAGCCTTGGTAACCGGCATTGCCAACGAGCGCTCCATTGCCTGGGGCATTGCTCAGCA
ACTGCACAAAGCGGGGGCCAGCCTAGGGGTTACCTATCTGCCGGACGAGCGGGATCGCTTCAAAACCAAAGTTCAGCAAC
TCACGGATCCCTTGCAGCCAGATTTTCTTCTCCCCTGCGACGTGCAAAGCGATCAGCAGATCAGCGATCTGTTTCAGGGG
GTAACCGAAAAATGGGGTCAGGTCGATATTCTGGTTCACTGCCTGGCCTATGTCAAAAAAGAGGAGCTGAGCGGCAACTT
CAGCGATATCTCGCGGGAGGGGTTTGCCCTGGCCATGGATGTGAGCACCTACTCCCTGATTGCCCTCTGCCGGGCTGCCA
AGCCCCTACTGAGATCCGGCAGCAGTGTCCTCACCTTGACCTACTTGGGAGGAGTGCGAGTGGTGCCCAACTACAACATG
GCTGCCGTCACCAAAGCGGGCCTGGAAACCTGTGTCCGCTACCTGGCTGCCGAGCTGGGCCCTGCCGGGATCCGGGTGAA
CGCCATTTCTGCCGGCCCAATCCGCACCTTGGCCTCATCGGCCATTTCCGATTTTCATGCCATGCTGCATGCTGTGGAGG
AGAAAGCTCCCCTACGGCGCAATGTCACTCAAATAGAGGTGGGCAATGTGGCGGCTTTTCTGGCCAGCGACCTGGCCAGC
GGCATTACCGGCCAGGTGATCTACGTGGATAGTGGGTATAGCATCCTGGGTTTTTAA

Upstream 100 bases:

>100_bases
CCTTTTTAACGGGAGTTCAGGAGTGCCTGGGATCCCTTCTCCCAGCCCCAGCTCCCACCTGTGGGAGGATAGAAAGGAAT
ACACCCCTACTTTTTTTGCC

Downstream 100 bases:

>100_bases
GCGCTTTGGGATCCCTTGGGGATGGCTCCGGCATGAATCGCTTCTCACCCTTGCAGCGGTTGCTGCTCACCTGGATCTTG
ATCGTCATCAGCGGCTGGCT

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLQLTGKKALVTGIANERSIAWGIAQQLHKAGASLGVTYLPDERDRFKTKVQQLTDPLQPDFLLPCDVQSDQQISDLFQG
VTEKWGQVDILVHCLAYVKKEELSGNFSDISREGFALAMDVSTYSLIALCRAAKPLLRSGSSVLTLTYLGGVRVVPNYNM
AAVTKAGLETCVRYLAAELGPAGIRVNAISAGPIRTLASSAISDFHAMLHAVEEKAPLRRNVTQIEVGNVAAFLASDLAS
GITGQVIYVDSGYSILGF

Sequences:

>Translated_258_residues
MLQLTGKKALVTGIANERSIAWGIAQQLHKAGASLGVTYLPDERDRFKTKVQQLTDPLQPDFLLPCDVQSDQQISDLFQG
VTEKWGQVDILVHCLAYVKKEELSGNFSDISREGFALAMDVSTYSLIALCRAAKPLLRSGSSVLTLTYLGGVRVVPNYNM
AAVTKAGLETCVRYLAAELGPAGIRVNAISAGPIRTLASSAISDFHAMLHAVEEKAPLRRNVTQIEVGNVAAFLASDLAS
GITGQVIYVDSGYSILGF
>Mature_258_residues
MLQLTGKKALVTGIANERSIAWGIAQQLHKAGASLGVTYLPDERDRFKTKVQQLTDPLQPDFLLPCDVQSDQQISDLFQG
VTEKWGQVDILVHCLAYVKKEELSGNFSDISREGFALAMDVSTYSLIALCRAAKPLLRSGSSVLTLTYLGGVRVVPNYNM
AAVTKAGLETCVRYLAAELGPAGIRVNAISAGPIRTLASSAISDFHAMLHAVEEKAPLRRNVTQIEVGNVAAFLASDLAS
GITGQVIYVDSGYSILGF

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI10190704, Length=196, Percent_Identity=29.0816326530612, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI32483357, Length=255, Percent_Identity=27.0588235294118, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI19923817, Length=275, Percent_Identity=24, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI126723750, Length=255, Percent_Identity=27.0588235294118, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787545, Length=253, Percent_Identity=47.4308300395257, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI87082100, Length=268, Percent_Identity=29.1044776119403, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789378, Length=245, Percent_Identity=26.1224489795918, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787905, Length=258, Percent_Identity=29.8449612403101, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI2367365, Length=256, Percent_Identity=28.515625, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787335, Length=270, Percent_Identity=28.5185185185185, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=269, Percent_Identity=27.8810408921933, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562906, Length=271, Percent_Identity=27.6752767527675, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536025, Length=264, Percent_Identity=31.4393939393939, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17531453, Length=271, Percent_Identity=26.9372693726937, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=195, Percent_Identity=26.6666666666667, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=267, Percent_Identity=25.8426966292135, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=195, Percent_Identity=28.2051282051282, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17561272, Length=245, Percent_Identity=28.9795918367347, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=271, Percent_Identity=26.1992619926199, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=268, Percent_Identity=26.4925373134328, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324126, Length=196, Percent_Identity=27.5510204081633, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21355319, Length=257, Percent_Identity=28.4046692607004, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI23397609, Length=261, Percent_Identity=26.8199233716475, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357041, Length=264, Percent_Identity=28.030303030303, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24643142, Length=253, Percent_Identity=27.6679841897233, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571526, Length=201, Percent_Identity=27.363184079602, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27701; Mature: 27701

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQLTGKKALVTGIANERSIAWGIAQQLHKAGASLGVTYLPDERDRFKTKVQQLTDPLQP
CCEECCCCEEEEECCCCCHHHHHHHHHHHHCCHHCCEEECCCHHHHHHHHHHHHHCCCCC
DFLLPCDVQSDQQISDLFQGVTEKWGQVDILVHCLAYVKKEELSGNFSDISREGFALAMD
CEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEE
VSTYSLIALCRAAKPLLRSGSSVLTLTYLGGVRVVPNYNMAAVTKAGLETCVRYLAAELG
CHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHCC
PAGIRVNAISAGPIRTLASSAISDFHAMLHAVEEKAPLRRNVTQIEVGNVAAFLASDLAS
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEHHHHHHHHHHHHHC
GITGQVIYVDSGYSILGF
CCCCEEEEEECCCEECCC
>Mature Secondary Structure
MLQLTGKKALVTGIANERSIAWGIAQQLHKAGASLGVTYLPDERDRFKTKVQQLTDPLQP
CCEECCCCEEEEECCCCCHHHHHHHHHHHHCCHHCCEEECCCHHHHHHHHHHHHHCCCCC
DFLLPCDVQSDQQISDLFQGVTEKWGQVDILVHCLAYVKKEELSGNFSDISREGFALAMD
CEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEE
VSTYSLIALCRAAKPLLRSGSSVLTLTYLGGVRVVPNYNMAAVTKAGLETCVRYLAAELG
CHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHCC
PAGIRVNAISAGPIRTLASSAISDFHAMLHAVEEKAPLRRNVTQIEVGNVAAFLASDLAS
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEHHHHHHHHHHHHHC
GITGQVIYVDSGYSILGF
CCCCEEEEEECCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8391534; 11759840 [H]