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The map label for this gene is pdhC [H]

Identifier: 86608594

GI number: 86608594

Start: 1153962

End: 1155236

Strand: Direct

Name: pdhC [H]

Synonym: CYB_1116

Alternate gene names: 86608594

Gene position: 1153962-1155236 (Clockwise)

Preceding gene: 86608593

Following gene: 86608596

Centisome position: 37.88

GC content: 61.88

Gene sequence:

>1275_bases
ATGATCCACGAACTCTCCATGCCGGCCCTCAGCTCCACCATGGAAACGGGCAAGATCGTCACCTGGCTGAAAAACCCCGG
CGACCGCGTGGAGAAAGGAGAAAACATCCTGGTGGTGGAGTCCGACAAAGCGGACATGGACGTGGAGTCCTTCCACAGCG
GCATCCTGGCCAGTATCCTGGTGCCGGCAGGGGAATCAGCCCCGGTGGGCGCCCCCATTGCCCTGATTGCCGAAAGCGAA
GCGGAAGTGGCCCAAGCTCAGGAAAAAGCCAAAGCCCTCGCTGCTGGCGTTACCCCTGCGGCTCCACCTAGCGCAGACCG
CGCCTCTGCAGCTCAGCCGACAAGTCCTGCCCCTGCGGCAACCCCCACCTCTACACTGCCAAATGGATCGGATGGCGCCG
GATCCCAGCGGATCGTGGCTTCCCCGCGGGCGAAAAAATTGGCGGAAAGCCTGGGGATCGATCTGCGCACCGTGCGCGGC
TCCGGCCCCAACGGTCGCATCATAGCCGAAGACGTGGAGCGAGCTGCGGCCCTTTCCGCTCCTGCAGTGGCTGCCCCCTC
TGCTCCAGCCCCCGCGCCTCCAACTCCTGTAGCTGTCCCCTTGGGAGAAACGGTTCCCCTCAGCACTTTACAAGCGGCGG
TGGTGCGCAACATGAACGCCAGCCTTGGCGTGCCGGTGTTCCACGTGGGCTACACCATCACCACCGATAGCCTGGATCAC
CTCTACCAACAGGTAAAGCCTAAGGGGGTGACCCTGACGGCCCTCTTGGTCAAAGCCGTGGCCATGACCCTGGAGAAGCA
TCCCCTTTTGAATGCCAGCTACACAGAAGGCGGGATCCATTACAAATCGGATATCAACATCGCCGTGGCCGTGGCCATGG
AGGATGGCGGGCTGATCACCCCTGTCCTAAAGCAAGCCAACCGGCTCGATCTGTACGAGATCTCCCGCCGCTGGAAGGAT
CTGGTGGAGCGGGCGCGGCGCAAGCAACTGCAGCCGGAAGAGTACAATAGCGGCACCTTCACCCTCTCCAACCTGGGCAT
GTTCGGGGTGGATCGCTTCGATGCCATTTTGCCCCCCAACCAGGGATCCATTTTGGCCATCGGCGCTTCTCGCCCCACGG
TGGTGGCGACGCCGGAAAAAGCCATTGCCATCCGTTCCCAAATGCAGGTGAACCTCACCTGTGATCACCGTGTCATCTAC
GGCGCCCATGCAGCGGCTTTCCTGCAGGATCTGGCGCAGCTCATAGAGCACAAGGTGGGATCCCTGACGTTGTAG

Upstream 100 bases:

>100_bases
AGCGGCTGGCGCCCGGTGAGCCCAAAGCCGGCCCAGGGACGTGCGCGGGGATCCCGTCTCCTGGGTTACAATCTCTTCTG
CTGTGTTCCCATCCCCATCG

Downstream 100 bases:

>100_bases
CCGCCCTGAAACCCGGCCTTGAGCCAATCTCAGAGTAGGGTTCTTCTCTCGCGCAACCCACAAGGTCGGGCAGCCGGCGA
GGCCCCAGCGCTCAGGAGCT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MIHELSMPALSSTMETGKIVTWLKNPGDRVEKGENILVVESDKADMDVESFHSGILASILVPAGESAPVGAPIALIAESE
AEVAQAQEKAKALAAGVTPAAPPSADRASAAQPTSPAPAATPTSTLPNGSDGAGSQRIVASPRAKKLAESLGIDLRTVRG
SGPNGRIIAEDVERAAALSAPAVAAPSAPAPAPPTPVAVPLGETVPLSTLQAAVVRNMNASLGVPVFHVGYTITTDSLDH
LYQQVKPKGVTLTALLVKAVAMTLEKHPLLNASYTEGGIHYKSDINIAVAVAMEDGGLITPVLKQANRLDLYEISRRWKD
LVERARRKQLQPEEYNSGTFTLSNLGMFGVDRFDAILPPNQGSILAIGASRPTVVATPEKAIAIRSQMQVNLTCDHRVIY
GAHAAAFLQDLAQLIEHKVGSLTL

Sequences:

>Translated_424_residues
MIHELSMPALSSTMETGKIVTWLKNPGDRVEKGENILVVESDKADMDVESFHSGILASILVPAGESAPVGAPIALIAESE
AEVAQAQEKAKALAAGVTPAAPPSADRASAAQPTSPAPAATPTSTLPNGSDGAGSQRIVASPRAKKLAESLGIDLRTVRG
SGPNGRIIAEDVERAAALSAPAVAAPSAPAPAPPTPVAVPLGETVPLSTLQAAVVRNMNASLGVPVFHVGYTITTDSLDH
LYQQVKPKGVTLTALLVKAVAMTLEKHPLLNASYTEGGIHYKSDINIAVAVAMEDGGLITPVLKQANRLDLYEISRRWKD
LVERARRKQLQPEEYNSGTFTLSNLGMFGVDRFDAILPPNQGSILAIGASRPTVVATPEKAIAIRSQMQVNLTCDHRVIY
GAHAAAFLQDLAQLIEHKVGSLTL
>Mature_424_residues
MIHELSMPALSSTMETGKIVTWLKNPGDRVEKGENILVVESDKADMDVESFHSGILASILVPAGESAPVGAPIALIAESE
AEVAQAQEKAKALAAGVTPAAPPSADRASAAQPTSPAPAATPTSTLPNGSDGAGSQRIVASPRAKKLAESLGIDLRTVRG
SGPNGRIIAEDVERAAALSAPAVAAPSAPAPAPPTPVAVPLGETVPLSTLQAAVVRNMNASLGVPVFHVGYTITTDSLDH
LYQQVKPKGVTLTALLVKAVAMTLEKHPLLNASYTEGGIHYKSDINIAVAVAMEDGGLITPVLKQANRLDLYEISRRWKD
LVERARRKQLQPEEYNSGTFTLSNLGMFGVDRFDAILPPNQGSILAIGASRPTVVATPEKAIAIRSQMQVNLTCDHRVIY
GAHAAAFLQDLAQLIEHKVGSLTL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=438, Percent_Identity=33.3333333333333, Blast_Score=205, Evalue=8e-53,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=30.2631578947368, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI203098753, Length=459, Percent_Identity=30.0653594771242, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI110671329, Length=413, Percent_Identity=28.0871670702179, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI260898739, Length=178, Percent_Identity=37.0786516853933, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI19923748, Length=165, Percent_Identity=35.1515151515151, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786946, Length=423, Percent_Identity=26.4775413711584, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1786305, Length=405, Percent_Identity=29.1358024691358, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17560088, Length=437, Percent_Identity=33.8672768878719, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17537937, Length=417, Percent_Identity=28.2973621103118, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=295, Percent_Identity=31.5254237288136, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25146366, Length=179, Percent_Identity=35.7541899441341, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6324258, Length=448, Percent_Identity=35.2678571428571, Blast_Score=192, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6320352, Length=418, Percent_Identity=25.3588516746411, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6321632, Length=177, Percent_Identity=36.1581920903955, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI20129315, Length=428, Percent_Identity=34.3457943925234, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24582497, Length=421, Percent_Identity=33.729216152019, Blast_Score=170, Evalue=1e-42,
Organism=Drosophila melanogaster, GI18859875, Length=413, Percent_Identity=26.634382566586, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24645909, Length=168, Percent_Identity=32.7380952380952, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44362; Mature: 44362

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHELSMPALSSTMETGKIVTWLKNPGDRVEKGENILVVESDKADMDVESFHSGILASIL
CCCCCCCCHHHHHHCCCCEEEEECCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
VPAGESAPVGAPIALIAESEAEVAQAQEKAKALAAGVTPAAPPSADRASAAQPTSPAPAA
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
TPTSTLPNGSDGAGSQRIVASPRAKKLAESLGIDLRTVRGSGPNGRIIAEDVERAAALSA
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCEEEEEECCCCCCCEEHHHHHHHHHHCC
PAVAAPSAPAPAPPTPVAVPLGETVPLSTLQAAVVRNMNASLGVPVFHVGYTITTDSLDH
CCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEECEEEECHHHHH
LYQQVKPKGVTLTALLVKAVAMTLEKHPLLNASYTEGGIHYKSDINIAVAVAMEDGGLIT
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEEECCCCEEH
PVLKQANRLDLYEISRRWKDLVERARRKQLQPEEYNSGTFTLSNLGMFGVDRFDAILPPN
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHCCCCCC
QGSILAIGASRPTVVATPEKAIAIRSQMQVNLTCDHRVIYGAHAAAFLQDLAQLIEHKVG
CCCEEEEECCCCEEEECCHHHHEEECCEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHC
SLTL
CCCC
>Mature Secondary Structure
MIHELSMPALSSTMETGKIVTWLKNPGDRVEKGENILVVESDKADMDVESFHSGILASIL
CCCCCCCCHHHHHHCCCCEEEEECCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
VPAGESAPVGAPIALIAESEAEVAQAQEKAKALAAGVTPAAPPSADRASAAQPTSPAPAA
CCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
TPTSTLPNGSDGAGSQRIVASPRAKKLAESLGIDLRTVRGSGPNGRIIAEDVERAAALSA
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCEEEEEECCCCCCCEEHHHHHHHHHHCC
PAVAAPSAPAPAPPTPVAVPLGETVPLSTLQAAVVRNMNASLGVPVFHVGYTITTDSLDH
CCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHCCCCCCCCEEEEECEEEECHHHHH
LYQQVKPKGVTLTALLVKAVAMTLEKHPLLNASYTEGGIHYKSDINIAVAVAMEDGGLIT
HHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEEECCCCEEH
PVLKQANRLDLYEISRRWKDLVERARRKQLQPEEYNSGTFTLSNLGMFGVDRFDAILPPN
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHCCCCCC
QGSILAIGASRPTVVATPEKAIAIRSQMQVNLTCDHRVIYGAHAAAFLQDLAQLIEHKVG
CCCEEEEECCCCEEEECCHHHHEEECCEEEEEEECCEEEEHHHHHHHHHHHHHHHHHHHC
SLTL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]