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The map label for this gene is ppsA [H]

Identifier: 86608593

GI number: 86608593

Start: 1151415

End: 1153880

Strand: Direct

Name: ppsA [H]

Synonym: CYB_1115

Alternate gene names: 86608593

Gene position: 1151415-1153880 (Clockwise)

Preceding gene: 86608592

Following gene: 86608594

Centisome position: 37.79

GC content: 62.29

Gene sequence:

>2466_bases
ATGTCGGATGGAGCAACCAGGGGATCCCAGCTTCAGCAACCCTCTCAACCCCAGCTTGGCTCAGAGCCGCCTTTGGTCTT
GCCCCTGACGGAGGTGGGGCTGCGGGACGTTGCCCAGGTGGGCGGGAAAAACGCCTCCTTGGGAGAAATGCTGCAGCAGC
TCACCTCAAAGGGGATCCAGGTGCCGACAGGATTTGCCACCACCGCCCATGCGTATCGCCAGTTCATCGCCTCTGCGGGC
CTAGAGGAGAAGTTGCAGGAGATTTTCAAAGACTTAGACATTGAGAATGTCCAAAATTTGCGCGAGCGGGGCAAGGCTGC
CCGCACCTTGATCTTGCAAACTCCCTTCCCCCCTCAGCTAGAGCAGGCCATCGCCGAAGCCTACCAAAAGCTGTGCGAGC
GCTACGGCCCCGACACCGATGTGGCGGTGCGCTCCAGTGCCACGGCAGAAGACTTGCCGGATGCCAGCTTTGCCGGCCAG
CAGGAGACCTACCTGAACGTGCGTGGAGTGCGGGCGGTGCTCAACGCCTGCCACCACTGTTTTGCCAGCCTGTTTACCGA
CCGGGCCATTTCCTACCGCCAAATCAAGGGCTTTGACCATTTTCAAGTAGCCCTCTCGGTGGGGGTGCAGAAGATGGTGC
GCTCGGATCTGGCTTGCTCGGGGGTGATGTTCTCCATCGACCCAGAAACCGGCTTTAAAGATGCGGTGCTGATCACCGCC
GCCTACGGCCTGGGGGAAAACGTGGTGCAAGGGATCGTCAACCCCGATGAGTACGTGGTCTTCAAACCCACCCTCAAGCA
GGGACTGCGCCCCATCCTCTCCCGCAAGCTGGGCAGCAAGGCCCTGAAGCTGGTCTACGACGAGGGGGGATCCCGTTCCA
CCAAAAACGTGGCTGTCCCCGAAAGCCTGCGCAAGCAATATGCCCTCAGGGATGAGGAAATCCTCAAATTGGCCCAGTGG
GCCTGCTTGATCGAGGAGCACTACTCGGCACAGCGGGGCCGCTTCACCCCCATGGACATCGAGTGGGCCAAAGATGGGAT
CACCGGCGAGCTGTTTATCGTCCAGGCCCGCCCCGAAACAGTGCAGTCGCAAAAAGGGGCCAACCAGCTGACCACCTATC
GCTTCACCCAGCCTGGGGCGCAGCCCGAGCCTCTGGTGCGGGGACGGGCCGTAGGCGAGGCCATTGGCCAGGGCACAGCC
CGCGTCATTTTGGATGTGCGCCGCTCGGATCAGTTTCAGGCCGGCGAGGTGCTGGTGACCGAGAAGACGGATCCCGACTG
GGAGCCCATCATGAAGAAAGCCAGCGCCATCGTCACCAACCAGGGGGGCAGAACTTGCCATGCGGCCATCATCGCCCGCG
AGCTGGGGATCCCGGCCATTGTTGGCTGCGGCAACGCCACGCAGGTGCTCCGTTCCGGCGATCCCATCACCGTCTCCTGC
GCTGAAGGGGAAGAAGGGCGGGTCTACCCGGGCCTGTTGCCCTATGAGGTGACGGAGATCCCGCTGGAGGAGCTGCCCCG
TCCCCGCACCCAGCTGATGATGAATGTGGGCAACCCAGAAAAAGCCTTCGGCCTCAGCGGGATCCCTTGCGATGGCGTGG
GGCTGGCGCGGCTAGAGTTCATCATTGCCAACCACATTCAGGTCCACCCGCTGGCTTTGCTCAACTATGAGCAGGCGGTG
AAAGAGGAGCCGCGCCTGGCAGAGCTCACCGCCCAGTATGCCGACAAGCCGGAGTACTTTGTGGACAAGCTGGCCCAGGG
GGTGGCCACCATTGCTGCCGCCTTTTACCCCAAGCCGGTGATCGTGCGGCTGTCGGATTTCAAGAGCAACGAGTATGCCC
ACCTGTTGGGGGGATCCCGCTACGAACCCCACGAGGAAAACCCCATGATTGGCTGGCGGGGGGCCTCCCGCTACACGGAT
CCCCGCTACCGTGAGGGCTTTGCCTTGGAGTGTCGGGCCATGAAACGGGTGCGGGATGAGATGGGCCTGACCAACGTCAT
CTTGATGATCCCCTTCTGCCGCACGCCGGAAGAGGGGCACAAGGTGCTGGAGGAGATGGCCCGCCATGGCCTGGTGCGGG
GAGAAAACGGCCTGCAGGTGTACGTGATGTGCGAATTGCCCAGCAATGTTATCCTGGCCGACGAGTTCTGCCAGATCTTC
GACGGCTTCTCCATCGGCTCCAACGACCTCACCCAGCTCACTCTGGGCCTGGATCGCGACTCAGCCCTGGTGGCCCACCT
TTTTGACGAGCGCCACGAGGCCGTCAGGCGGATGATCGCCCAGGTAATCCAGACGGTGAAAAGCCATGGCCGCAAGATCG
GCATCTGCGGGCAGGCCCCCAGCGACTACCCCGACTTCGCCCGCTTCCTGGTGCAAGCAGGGATCGATTCCATCAGCCTC
AACCCCGATTCCCTGTTGAAAACCCTGCTGGTGGTGGCCGAAGCAGAAGCGGCTGGCGCCCGGTGA

Upstream 100 bases:

>100_bases
TAGGAGAGCCGGGAGCGATCCCAAGCCGACAACACAGCACACCCCAGCAATTGGCGTTGGCTCAATGGATCGCTCAATGG
ATCTAAGAAGGAGGTAAGGC

Downstream 100 bases:

>100_bases
GCCCAAAGCCGGCCCAGGGACGTGCGCGGGGATCCCGTCTCCTGGGTTACAATCTCTTCTGCTGTGTTCCCATCCCCATC
GATGATCCACGAACTCTCCA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 821; Mature: 820

Protein sequence:

>821_residues
MSDGATRGSQLQQPSQPQLGSEPPLVLPLTEVGLRDVAQVGGKNASLGEMLQQLTSKGIQVPTGFATTAHAYRQFIASAG
LEEKLQEIFKDLDIENVQNLRERGKAARTLILQTPFPPQLEQAIAEAYQKLCERYGPDTDVAVRSSATAEDLPDASFAGQ
QETYLNVRGVRAVLNACHHCFASLFTDRAISYRQIKGFDHFQVALSVGVQKMVRSDLACSGVMFSIDPETGFKDAVLITA
AYGLGENVVQGIVNPDEYVVFKPTLKQGLRPILSRKLGSKALKLVYDEGGSRSTKNVAVPESLRKQYALRDEEILKLAQW
ACLIEEHYSAQRGRFTPMDIEWAKDGITGELFIVQARPETVQSQKGANQLTTYRFTQPGAQPEPLVRGRAVGEAIGQGTA
RVILDVRRSDQFQAGEVLVTEKTDPDWEPIMKKASAIVTNQGGRTCHAAIIARELGIPAIVGCGNATQVLRSGDPITVSC
AEGEEGRVYPGLLPYEVTEIPLEELPRPRTQLMMNVGNPEKAFGLSGIPCDGVGLARLEFIIANHIQVHPLALLNYEQAV
KEEPRLAELTAQYADKPEYFVDKLAQGVATIAAAFYPKPVIVRLSDFKSNEYAHLLGGSRYEPHEENPMIGWRGASRYTD
PRYREGFALECRAMKRVRDEMGLTNVILMIPFCRTPEEGHKVLEEMARHGLVRGENGLQVYVMCELPSNVILADEFCQIF
DGFSIGSNDLTQLTLGLDRDSALVAHLFDERHEAVRRMIAQVIQTVKSHGRKIGICGQAPSDYPDFARFLVQAGIDSISL
NPDSLLKTLLVVAEAEAAGAR

Sequences:

>Translated_821_residues
MSDGATRGSQLQQPSQPQLGSEPPLVLPLTEVGLRDVAQVGGKNASLGEMLQQLTSKGIQVPTGFATTAHAYRQFIASAG
LEEKLQEIFKDLDIENVQNLRERGKAARTLILQTPFPPQLEQAIAEAYQKLCERYGPDTDVAVRSSATAEDLPDASFAGQ
QETYLNVRGVRAVLNACHHCFASLFTDRAISYRQIKGFDHFQVALSVGVQKMVRSDLACSGVMFSIDPETGFKDAVLITA
AYGLGENVVQGIVNPDEYVVFKPTLKQGLRPILSRKLGSKALKLVYDEGGSRSTKNVAVPESLRKQYALRDEEILKLAQW
ACLIEEHYSAQRGRFTPMDIEWAKDGITGELFIVQARPETVQSQKGANQLTTYRFTQPGAQPEPLVRGRAVGEAIGQGTA
RVILDVRRSDQFQAGEVLVTEKTDPDWEPIMKKASAIVTNQGGRTCHAAIIARELGIPAIVGCGNATQVLRSGDPITVSC
AEGEEGRVYPGLLPYEVTEIPLEELPRPRTQLMMNVGNPEKAFGLSGIPCDGVGLARLEFIIANHIQVHPLALLNYEQAV
KEEPRLAELTAQYADKPEYFVDKLAQGVATIAAAFYPKPVIVRLSDFKSNEYAHLLGGSRYEPHEENPMIGWRGASRYTD
PRYREGFALECRAMKRVRDEMGLTNVILMIPFCRTPEEGHKVLEEMARHGLVRGENGLQVYVMCELPSNVILADEFCQIF
DGFSIGSNDLTQLTLGLDRDSALVAHLFDERHEAVRRMIAQVIQTVKSHGRKIGICGQAPSDYPDFARFLVQAGIDSISL
NPDSLLKTLLVVAEAEAAGAR
>Mature_820_residues
SDGATRGSQLQQPSQPQLGSEPPLVLPLTEVGLRDVAQVGGKNASLGEMLQQLTSKGIQVPTGFATTAHAYRQFIASAGL
EEKLQEIFKDLDIENVQNLRERGKAARTLILQTPFPPQLEQAIAEAYQKLCERYGPDTDVAVRSSATAEDLPDASFAGQQ
ETYLNVRGVRAVLNACHHCFASLFTDRAISYRQIKGFDHFQVALSVGVQKMVRSDLACSGVMFSIDPETGFKDAVLITAA
YGLGENVVQGIVNPDEYVVFKPTLKQGLRPILSRKLGSKALKLVYDEGGSRSTKNVAVPESLRKQYALRDEEILKLAQWA
CLIEEHYSAQRGRFTPMDIEWAKDGITGELFIVQARPETVQSQKGANQLTTYRFTQPGAQPEPLVRGRAVGEAIGQGTAR
VILDVRRSDQFQAGEVLVTEKTDPDWEPIMKKASAIVTNQGGRTCHAAIIARELGIPAIVGCGNATQVLRSGDPITVSCA
EGEEGRVYPGLLPYEVTEIPLEELPRPRTQLMMNVGNPEKAFGLSGIPCDGVGLARLEFIIANHIQVHPLALLNYEQAVK
EEPRLAELTAQYADKPEYFVDKLAQGVATIAAAFYPKPVIVRLSDFKSNEYAHLLGGSRYEPHEENPMIGWRGASRYTDP
RYREGFALECRAMKRVRDEMGLTNVILMIPFCRTPEEGHKVLEEMARHGLVRGENGLQVYVMCELPSNVILADEFCQIFD
GFSIGSNDLTQLTLGLDRDSALVAHLFDERHEAVRRMIAQVIQTVKSHGRKIGICGQAPSDYPDFARFLVQAGIDSISLN
PDSLLKTLLVVAEAEAAGAR

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=797, Percent_Identity=55.5834378920954, Blast_Score=888, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=403, Percent_Identity=26.0545905707196, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789193, Length=423, Percent_Identity=26.7139479905437, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788726, Length=377, Percent_Identity=25.1989389920424, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI48994992, Length=369, Percent_Identity=26.8292682926829, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17564524, Length=236, Percent_Identity=29.6610169491525, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 90169; Mature: 90038

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGATRGSQLQQPSQPQLGSEPPLVLPLTEVGLRDVAQVGGKNASLGEMLQQLTSKGIQ
CCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
VPTGFATTAHAYRQFIASAGLEEKLQEIFKDLDIENVQNLRERGKAARTLILQTPFPPQL
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCHHHEEEEEECCCCHHH
EQAIAEAYQKLCERYGPDTDVAVRSSATAEDLPDASFAGQQETYLNVRGVRAVLNACHHC
HHHHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCCCHHEEHHHHHHHHHHHHHHH
FASLFTDRAISYRQIKGFDHFQVALSVGVQKMVRSDLACSGVMFSIDPETGFKDAVLITA
HHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEE
AYGLGENVVQGIVNPDEYVVFKPTLKQGLRPILSRKLGSKALKLVYDEGGSRSTKNVAVP
ECCCCHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCCCC
ESLRKQYALRDEEILKLAQWACLIEEHYSAQRGRFTPMDIEWAKDGITGELFIVQARPET
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEEEECCCHH
VQSQKGANQLTTYRFTQPGAQPEPLVRGRAVGEAIGQGTARVILDVRRSDQFQAGEVLVT
HHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEE
EKTDPDWEPIMKKASAIVTNQGGRTCHAAIIARELGIPAIVGCGNATQVLRSGDPITVSC
CCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCEEEEE
AEGEEGRVYPGLLPYEVTEIPLEELPRPRTQLMMNVGNPEKAFGLSGIPCDGVGLARLEF
CCCCCCCCCCCCCCCHHHCCCHHHCCCCHHHHEEECCCCHHHCCCCCCCCCCCHHHHHHH
IIANHIQVHPLALLNYEQAVKEEPRLAELTAQYADKPEYFVDKLAQGVATIAAAFYPKPV
HHHCCCEECEEHHHCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
IVRLSDFKSNEYAHLLGGSRYEPHEENPMIGWRGASRYTDPRYREGFALECRAMKRVRDE
EEEEECCCCCCCHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
MGLTNVILMIPFCRTPEEGHKVLEEMARHGLVRGENGLQVYVMCELPSNVILADEFCQIF
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEHHHHHHHH
DGFSIGSNDLTQLTLGLDRDSALVAHLFDERHEAVRRMIAQVIQTVKSHGRKIGICGQAP
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
SDYPDFARFLVQAGIDSISLNPDSLLKTLLVVAEAEAAGAR
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure 
SDGATRGSQLQQPSQPQLGSEPPLVLPLTEVGLRDVAQVGGKNASLGEMLQQLTSKGIQ
CCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
VPTGFATTAHAYRQFIASAGLEEKLQEIFKDLDIENVQNLRERGKAARTLILQTPFPPQL
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCHHHEEEEEECCCCHHH
EQAIAEAYQKLCERYGPDTDVAVRSSATAEDLPDASFAGQQETYLNVRGVRAVLNACHHC
HHHHHHHHHHHHHHHCCCCCEEEECCCCHHCCCCCCCCCCCHHEEHHHHHHHHHHHHHHH
FASLFTDRAISYRQIKGFDHFQVALSVGVQKMVRSDLACSGVMFSIDPETGFKDAVLITA
HHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEE
AYGLGENVVQGIVNPDEYVVFKPTLKQGLRPILSRKLGSKALKLVYDEGGSRSTKNVAVP
ECCCCHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHCHHHEEEEEECCCCCCCCCCCCC
ESLRKQYALRDEEILKLAQWACLIEEHYSAQRGRFTPMDIEWAKDGITGELFIVQARPET
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCEEEEEEECCCHH
VQSQKGANQLTTYRFTQPGAQPEPLVRGRAVGEAIGQGTARVILDVRRSDQFQAGEVLVT
HHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEE
EKTDPDWEPIMKKASAIVTNQGGRTCHAAIIARELGIPAIVGCGNATQVLRSGDPITVSC
CCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCEEEEE
AEGEEGRVYPGLLPYEVTEIPLEELPRPRTQLMMNVGNPEKAFGLSGIPCDGVGLARLEF
CCCCCCCCCCCCCCCHHHCCCHHHCCCCHHHHEEECCCCHHHCCCCCCCCCCCHHHHHHH
IIANHIQVHPLALLNYEQAVKEEPRLAELTAQYADKPEYFVDKLAQGVATIAAAFYPKPV
HHHCCCEECEEHHHCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCE
IVRLSDFKSNEYAHLLGGSRYEPHEENPMIGWRGASRYTDPRYREGFALECRAMKRVRDE
EEEEECCCCCCCHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
MGLTNVILMIPFCRTPEEGHKVLEEMARHGLVRGENGLQVYVMCELPSNVILADEFCQIF
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEHHHHHHHH
DGFSIGSNDLTQLTLGLDRDSALVAHLFDERHEAVRRMIAQVIQTVKSHGRKIGICGQAP
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCC
SDYPDFARFLVQAGIDSISLNPDSLLKTLLVVAEAEAAGAR
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]