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The map label for this gene is rpoD [H]

Identifier: 86608149

GI number: 86608149

Start: 675947

End: 677032

Strand: Reverse

Name: rpoD [H]

Synonym: CYB_0662

Alternate gene names: 86608149

Gene position: 677032-675947 (Counterclockwise)

Preceding gene: 86608152

Following gene: 86608148

Centisome position: 22.22

GC content: 58.75

Gene sequence:

>1086_bases
GTGGTTTTTTCTCGTCCTCATCCTGAACACTCTGTCTCTTCGCCTCAGGAGTTCGACGATCTCTGGTTCGACCCCGAGTC
TGCCCCTCAAGACGACCTCGCAGAAGTTGACTCAGAGCCAGGGGCCGGCGGATCCCGGTTGAATGAAGACCCTGTCGATC
ACTACCTGAATACCATCGGTCGCGTGCCCCGCATCGATCACGCTGAGGAAATCGAGTTGTCGCGCAAAATTCAGCAAAAG
CTCCTCATCGACCAAGCCCGGCAGGAATGGCGGCAACAGCGGGGGGAGGATCCCAGTGACGAGGCTTTGGCCGAGGCCCT
GCAGATTTCCGTATCCACCCTACGCTCCCGCATCCGACAAGGGGAGCAGGCTCAGCGCAAGCTGATCAACGCCAACCTGC
GCCTGGTGGTCAGCGTTGCCAAGAAATACCTCAATCGGGGGGTGCCGTTTTTGGATCTGATCCAAGAGGGCAATATCGGG
TTGATCCGGGCCACCGAAAAATTCAACGCCGAACGGGGGTTTCGCTTCAGCACCTACGCCCATTGGTGGATTCGCCAAGG
CATTACCCGCTGCATTGCCAACCAAGCGCGGACGATTCGCCTGCCGGTACACATGGTCGACAAAGTACGCCTGCTCAAGC
GCACCAACCGCGAGCTGATGAAGCTGCAGGGGAGGCGGCCCACCGAAGCGGAGCTGGCAGAAGCCTTGGGCATTAAAGTC
AAGAAACTGCGCATGATCCAACAGGCTGCTGCTTTGCCCCTGTCGCTGGATGTGCCGGTCGGCCAGGAAGGAGAAAGCCG
CCTTGGAGATTTGCTGCAAGATGAGCGGCAAGAGCAGCCCTTTCAAGGGATCCTCGTGCAATCGATGCAGCAGGATGTGC
GTTCTGCCATGCAAACCCTGAAGCCGATTGAGAGGCAAGTGCTGGAGTTGCGCTATGGGTTAGACGGGCAACAGGCGAGA
ACCCTGCGGGAGGTGGGGGATCACTTCAACCTAACGCGGGAACGGATTCGTCAGATCGAGCGGGATGCCCTGCGCAAATT
GCGGGTGGGCTATTCTTCGCGGGCTTTGGCCCAGTACATTCGCTAG

Upstream 100 bases:

>100_bases
AAAATCTCCGGACAAAAAAGAGGGGCATGATCGATTTTCGCTCGCAGCAGCAGCAGAAACCTTTTTAGACTGGGAGATGA
AGTTCTCTCCTCACCTGGCT

Downstream 100 bases:

>100_bases
TGGCTACAGGCCTGGCAGACCGATTTCCGGGCCAGCTTCTGCCCAGGGATAGCCTTTTTTCAATCTTCTTGGGTTGAACA
CCATAGCCCCTTGCAGCGGC

Product: RNA polymerase sigma factor, group 1

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MVFSRPHPEHSVSSPQEFDDLWFDPESAPQDDLAEVDSEPGAGGSRLNEDPVDHYLNTIGRVPRIDHAEEIELSRKIQQK
LLIDQARQEWRQQRGEDPSDEALAEALQISVSTLRSRIRQGEQAQRKLINANLRLVVSVAKKYLNRGVPFLDLIQEGNIG
LIRATEKFNAERGFRFSTYAHWWIRQGITRCIANQARTIRLPVHMVDKVRLLKRTNRELMKLQGRRPTEAELAEALGIKV
KKLRMIQQAAALPLSLDVPVGQEGESRLGDLLQDERQEQPFQGILVQSMQQDVRSAMQTLKPIERQVLELRYGLDGQQAR
TLREVGDHFNLTRERIRQIERDALRKLRVGYSSRALAQYIR

Sequences:

>Translated_361_residues
MVFSRPHPEHSVSSPQEFDDLWFDPESAPQDDLAEVDSEPGAGGSRLNEDPVDHYLNTIGRVPRIDHAEEIELSRKIQQK
LLIDQARQEWRQQRGEDPSDEALAEALQISVSTLRSRIRQGEQAQRKLINANLRLVVSVAKKYLNRGVPFLDLIQEGNIG
LIRATEKFNAERGFRFSTYAHWWIRQGITRCIANQARTIRLPVHMVDKVRLLKRTNRELMKLQGRRPTEAELAEALGIKV
KKLRMIQQAAALPLSLDVPVGQEGESRLGDLLQDERQEQPFQGILVQSMQQDVRSAMQTLKPIERQVLELRYGLDGQQAR
TLREVGDHFNLTRERIRQIERDALRKLRVGYSSRALAQYIR
>Mature_361_residues
MVFSRPHPEHSVSSPQEFDDLWFDPESAPQDDLAEVDSEPGAGGSRLNEDPVDHYLNTIGRVPRIDHAEEIELSRKIQQK
LLIDQARQEWRQQRGEDPSDEALAEALQISVSTLRSRIRQGEQAQRKLINANLRLVVSVAKKYLNRGVPFLDLIQEGNIG
LIRATEKFNAERGFRFSTYAHWWIRQGITRCIANQARTIRLPVHMVDKVRLLKRTNRELMKLQGRRPTEAELAEALGIKV
KKLRMIQQAAALPLSLDVPVGQEGESRLGDLLQDERQEQPFQGILVQSMQQDVRSAMQTLKPIERQVLELRYGLDGQQAR
TLREVGDHFNLTRERIRQIERDALRKLRVGYSSRALAQYIR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=254, Percent_Identity=46.0629921259843, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1789098, Length=283, Percent_Identity=43.1095406360424, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1789871, Length=244, Percent_Identity=29.5081967213115, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788231, Length=209, Percent_Identity=30.622009569378, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR017848
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41539; Mature: 41539

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFSRPHPEHSVSSPQEFDDLWFDPESAPQDDLAEVDSEPGAGGSRLNEDPVDHYLNTIG
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
RVPRIDHAEEIELSRKIQQKLLIDQARQEWRQQRGEDPSDEALAEALQISVSTLRSRIRQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GEQAQRKLINANLRLVVSVAKKYLNRGVPFLDLIQEGNIGLIRATEKFNAERGFRFSTYA
CHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECHHHHCCCCCCCHHHHH
HWWIRQGITRCIANQARTIRLPVHMVDKVRLLKRTNRELMKLQGRRPTEAELAEALGIKV
HHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHH
KKLRMIQQAAALPLSLDVPVGQEGESRLGDLLQDERQEQPFQGILVQSMQQDVRSAMQTL
HHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KPIERQVLELRYGLDGQQARTLREVGDHFNLTRERIRQIERDALRKLRVGYSSRALAQYI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
R
C
>Mature Secondary Structure
MVFSRPHPEHSVSSPQEFDDLWFDPESAPQDDLAEVDSEPGAGGSRLNEDPVDHYLNTIG
CCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHH
RVPRIDHAEEIELSRKIQQKLLIDQARQEWRQQRGEDPSDEALAEALQISVSTLRSRIRQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
GEQAQRKLINANLRLVVSVAKKYLNRGVPFLDLIQEGNIGLIRATEKFNAERGFRFSTYA
CHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECHHHHCCCCCCCHHHHH
HWWIRQGITRCIANQARTIRLPVHMVDKVRLLKRTNRELMKLQGRRPTEAELAEALGIKV
HHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHH
KKLRMIQQAAALPLSLDVPVGQEGESRLGDLLQDERQEQPFQGILVQSMQQDVRSAMQTL
HHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KPIERQVLELRYGLDGQQARTLREVGDHFNLTRERIRQIERDALRKLRVGYSSRALAQYI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1901566; 11759840 [H]