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The map label for this gene is ilvB [H]

Identifier: 86608136

GI number: 86608136

Start: 659644

End: 661440

Strand: Reverse

Name: ilvB [H]

Synonym: CYB_0648

Alternate gene names: 86608136

Gene position: 661440-659644 (Counterclockwise)

Preceding gene: 86608139

Following gene: 86608131

Centisome position: 21.71

GC content: 63.38

Gene sequence:

>1797_bases
GTGGCGACTGGTGGATATGCGTTGGTGGACAGCCTGCACCGGCATGGGGTGCAACACATCTTTGGCTATCCGGGGGGAGC
CATCCTCCCCATCTACGACGAGCTGTACAAAGCCGAGTCTCAGGGGCTGCTCAAACATTACCTGGTACGCCACGAACAAG
CGGCTGCCCACGCCGCCGACGGATATGCTCGCGCCACCGGCAAGGTGGGGGTGTGTTTTGCTACCTCTGGCCCAGGGGCG
ACCAACCTGGTGACGGGCATCGCCAACGCCATGATGGACTCTGTACCCATGGTGGTCATCACCGGGCAGGTGCCCCGCCA
TTTGATCGGCACCGACGGCTTTCAGGAGACCGACATCTTTGGCATCACCCTGCCCATCGTCAAGCACTCCTACGTGGTGC
GGGATCCGCGCCAAATGGCTCGGATCGTGGCCGAGGCTTTTCACATTGCTGGCACCGGGCGACCGGGGCCGGTGCTGATC
GACATTCCCAAAGATGTGGGGCTGGAAGAGTTCGACTACCAGCCGGTTAGCGAAGTCAACATCCCCGGCTACCGGCCCAC
GGTGCGGGGGAACCCGCGCCAGATCCACGCGGCGGCCAAGCTGATCCTGCAGGCCAAGCGGCCCCTGCTCTACGTGGGGG
GCGGGGCCATCATCTCGGGTGCCCATGCCGAGATTGCCGAGCTGGCCCACACCTATCAGATCCCGGTGACCACCACCCTC
ATGGGCAAAGGGGCCTTCGATGAGAACGATCCCCTCTCGGTGGGGATGCTGGGCATGCACGGCACCGCCTACGCCAACTT
CGCCGTTACCGAGTGCGACCTGCTGATTGCCGTGGGGGCCCGCTTTGACGACCGCGTAACCGGCAAGCTGGACGAATTTG
CCAAGCACGCCAAGGTTATCCACATTGATATCGACCCGGCAGAAGTGGGCAAAAACCGCACTCCGGACGTGCCGATTGTG
GGAGATGTGCGCACCGTCCTGGTGGAGCTGTTGGCGCAACTGAGCAAAATGCCCCACACCCCTGGCCAGACGCAGGCTTG
GCTGGAGCGGATCGAGCGCTGGAAACGGGACTACCCCCTGCAGGTGCCCCGCTATGAGGGCGTGATCGCCCCGCAGCAGG
TGATCGATGCCTTTCGTCGCCATGCTCCCCACGCCTACTACACCACCGATGTCGGTCAGCACCAAATGTGGGCGGCGCAG
TTTCTGCGTAACGGGCCGCGCCAGTGGATCTCCAGTTCTGGCCTGGGCACCATGGGCTACGGTTTCCCGGCGGCCATGGG
GGTCAAAGTGGCCCTGCCCCACGCCGAGGTGATCTGCATCAGCGGCGACGCCAGCTTCCAGATGAACATGCAGGAGCTGG
GCACGGTGGCCCAGTACGGCATTGCCACCAAGGTGGCCATCATCAACAACGGCTGGCAGGGCATGGTGCGGCAGTGGCAG
CAGGCCTTCCACGGCGAGCGCTACTCCCACTCGGATATGACTCCGGGCATGCCGGATTTCGTCAAGCTGGCTGAAGCCTT
TGGCGTCAAGGGCATGCGCGTCGATCACCCCGATCAAGTGGACGACGCCATCCAAGAGATGCTGCGCCACGAGGGGCCGG
TGGTGGTCGATTTTGTGGTCAAGCGGGACGAGAACTGCTACCCGATGGTGCCCGCCGGCAAAGCCAACTACCAGATGATC
GGCCTGCCGGAAAAGCGGCAACTGGAGCAAGCCGCCGAGCTGATTTACTGCCCCAATTGCGGCACCAAAAACCCCAGCCT
GCACAAGTTCTGCGCCGAGTGCGGCACCAAGCTCTAG

Upstream 100 bases:

>100_bases
GAATACAAAGTTGGCCAACTTCTGGCCAGTACCCTGTCTGCGCTGGCGCTCCTGAGAGCGGGCGTTTGACGTTCGGTCAT
CTCAGTGGGGAGGAGACACC

Downstream 100 bases:

>100_bases
AATCGGTGGCCTGGCCATTCGGCTGGGGAAGGCGGGCCCCAGCAGCAGGGAGTCTTCGGGGATATCGGGGCTACTGAACA
AGCCAGATTAAAACGCTCCC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MATGGYALVDSLHRHGVQHIFGYPGGAILPIYDELYKAESQGLLKHYLVRHEQAAAHAADGYARATGKVGVCFATSGPGA
TNLVTGIANAMMDSVPMVVITGQVPRHLIGTDGFQETDIFGITLPIVKHSYVVRDPRQMARIVAEAFHIAGTGRPGPVLI
DIPKDVGLEEFDYQPVSEVNIPGYRPTVRGNPRQIHAAAKLILQAKRPLLYVGGGAIISGAHAEIAELAHTYQIPVTTTL
MGKGAFDENDPLSVGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDEFAKHAKVIHIDIDPAEVGKNRTPDVPIV
GDVRTVLVELLAQLSKMPHTPGQTQAWLERIERWKRDYPLQVPRYEGVIAPQQVIDAFRRHAPHAYYTTDVGQHQMWAAQ
FLRNGPRQWISSSGLGTMGYGFPAAMGVKVALPHAEVICISGDASFQMNMQELGTVAQYGIATKVAIINNGWQGMVRQWQ
QAFHGERYSHSDMTPGMPDFVKLAEAFGVKGMRVDHPDQVDDAIQEMLRHEGPVVVDFVVKRDENCYPMVPAGKANYQMI
GLPEKRQLEQAAELIYCPNCGTKNPSLHKFCAECGTKL

Sequences:

>Translated_598_residues
MATGGYALVDSLHRHGVQHIFGYPGGAILPIYDELYKAESQGLLKHYLVRHEQAAAHAADGYARATGKVGVCFATSGPGA
TNLVTGIANAMMDSVPMVVITGQVPRHLIGTDGFQETDIFGITLPIVKHSYVVRDPRQMARIVAEAFHIAGTGRPGPVLI
DIPKDVGLEEFDYQPVSEVNIPGYRPTVRGNPRQIHAAAKLILQAKRPLLYVGGGAIISGAHAEIAELAHTYQIPVTTTL
MGKGAFDENDPLSVGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDEFAKHAKVIHIDIDPAEVGKNRTPDVPIV
GDVRTVLVELLAQLSKMPHTPGQTQAWLERIERWKRDYPLQVPRYEGVIAPQQVIDAFRRHAPHAYYTTDVGQHQMWAAQ
FLRNGPRQWISSSGLGTMGYGFPAAMGVKVALPHAEVICISGDASFQMNMQELGTVAQYGIATKVAIINNGWQGMVRQWQ
QAFHGERYSHSDMTPGMPDFVKLAEAFGVKGMRVDHPDQVDDAIQEMLRHEGPVVVDFVVKRDENCYPMVPAGKANYQMI
GLPEKRQLEQAAELIYCPNCGTKNPSLHKFCAECGTKL
>Mature_597_residues
ATGGYALVDSLHRHGVQHIFGYPGGAILPIYDELYKAESQGLLKHYLVRHEQAAAHAADGYARATGKVGVCFATSGPGAT
NLVTGIANAMMDSVPMVVITGQVPRHLIGTDGFQETDIFGITLPIVKHSYVVRDPRQMARIVAEAFHIAGTGRPGPVLID
IPKDVGLEEFDYQPVSEVNIPGYRPTVRGNPRQIHAAAKLILQAKRPLLYVGGGAIISGAHAEIAELAHTYQIPVTTTLM
GKGAFDENDPLSVGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDEFAKHAKVIHIDIDPAEVGKNRTPDVPIVG
DVRTVLVELLAQLSKMPHTPGQTQAWLERIERWKRDYPLQVPRYEGVIAPQQVIDAFRRHAPHAYYTTDVGQHQMWAAQF
LRNGPRQWISSSGLGTMGYGFPAAMGVKVALPHAEVICISGDASFQMNMQELGTVAQYGIATKVAIINNGWQGMVRQWQQ
AFHGERYSHSDMTPGMPDFVKLAEAFGVKGMRVDHPDQVDDAIQEMLRHEGPVVVDFVVKRDENCYPMVPAGKANYQMIG
LPEKRQLEQAAELIYCPNCGTKNPSLHKFCAECGTKL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=545, Percent_Identity=26.2385321100917, Blast_Score=177, Evalue=3e-44,
Organism=Homo sapiens, GI21361361, Length=599, Percent_Identity=26.5442404006678, Blast_Score=165, Evalue=1e-40,
Organism=Escherichia coli, GI87081685, Length=558, Percent_Identity=46.7741935483871, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1790104, Length=561, Percent_Identity=42.602495543672, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1786717, Length=554, Percent_Identity=34.8375451263538, Blast_Score=335, Evalue=6e-93,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=29.4755877034358, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1788716, Length=554, Percent_Identity=25.9927797833935, Blast_Score=173, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17542570, Length=601, Percent_Identity=25.6239600665557, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=578, Percent_Identity=26.643598615917, Blast_Score=145, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=578, Percent_Identity=26.643598615917, Blast_Score=145, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=599, Percent_Identity=42.5709515859766, Blast_Score=448, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=510, Percent_Identity=26.4705882352941, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6323163, Length=522, Percent_Identity=23.5632183908046, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922626, Length=550, Percent_Identity=27.2727272727273, Blast_Score=171, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65377; Mature: 65246

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATGGYALVDSLHRHGVQHIFGYPGGAILPIYDELYKAESQGLLKHYLVRHEQAAAHAAD
CCCCHHHHHHHHHHCCHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GYARATGKVGVCFATSGPGATNLVTGIANAMMDSVPMVVITGQVPRHLIGTDGFQETDIF
CHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCCCEE
GITLPIVKHSYVVRDPRQMARIVAEAFHIAGTGRPGPVLIDIPKDVGLEEFDYQPVSEVN
EEEEHHHHCCCEECCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
IPGYRPTVRGNPRQIHAAAKLILQAKRPLLYVGGGAIISGAHAEIAELAHTYQIPVTTTL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEECCHHHHHHHHHHEECCEEEEE
MGKGAFDENDPLSVGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDEFAKHAKVI
EECCCCCCCCCCEEEEEECCCCEEECEEEECCEEEEEECCCCCCCCCCCHHHHHHCCEEE
HIDIDPAEVGKNRTPDVPIVGDVRTVLVELLAQLSKMPHTPGQTQAWLERIERWKRDYPL
EEECCHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
QVPRYEGVIAPQQVIDAFRRHAPHAYYTTDVGQHQMWAAQFLRNGPRQWISSSGLGTMGY
CCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCC
GFPAAMGVKVALPHAEVICISGDASFQMNMQELGTVAQYGIATKVAIINNGWQGMVRQWQ
CCHHHCCEEEECCCCEEEEEECCCCEEECHHHHHHHHHHCCEEEEEEEECCHHHHHHHHH
QAFHGERYSHSDMTPGMPDFVKLAEAFGVKGMRVDHPDQVDDAIQEMLRHEGPVVVDFVV
HHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEE
KRDENCYPMVPAGKANYQMIGLPEKRQLEQAAELIYCPNCGTKNPSLHKFCAECGTKL
ECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
ATGGYALVDSLHRHGVQHIFGYPGGAILPIYDELYKAESQGLLKHYLVRHEQAAAHAAD
CCCHHHHHHHHHHCCHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GYARATGKVGVCFATSGPGATNLVTGIANAMMDSVPMVVITGQVPRHLIGTDGFQETDIF
CHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCCCCCCCCEE
GITLPIVKHSYVVRDPRQMARIVAEAFHIAGTGRPGPVLIDIPKDVGLEEFDYQPVSEVN
EEEEHHHHCCCEECCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCC
IPGYRPTVRGNPRQIHAAAKLILQAKRPLLYVGGGAIISGAHAEIAELAHTYQIPVTTTL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEECCHHHHHHHHHHEECCEEEEE
MGKGAFDENDPLSVGMLGMHGTAYANFAVTECDLLIAVGARFDDRVTGKLDEFAKHAKVI
EECCCCCCCCCCEEEEEECCCCEEECEEEECCEEEEEECCCCCCCCCCCHHHHHHCCEEE
HIDIDPAEVGKNRTPDVPIVGDVRTVLVELLAQLSKMPHTPGQTQAWLERIERWKRDYPL
EEECCHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
QVPRYEGVIAPQQVIDAFRRHAPHAYYTTDVGQHQMWAAQFLRNGPRQWISSSGLGTMGY
CCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCC
GFPAAMGVKVALPHAEVICISGDASFQMNMQELGTVAQYGIATKVAIINNGWQGMVRQWQ
CCHHHCCEEEECCCCEEEEEECCCCEEECHHHHHHHHHHCCEEEEEEEECCHHHHHHHHH
QAFHGERYSHSDMTPGMPDFVKLAEAFGVKGMRVDHPDQVDDAIQEMLRHEGPVVVDFVV
HHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEE
KRDENCYPMVPAGKANYQMIGLPEKRQLEQAAELIYCPNCGTKNPSLHKFCAECGTKL
ECCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHEEECCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]