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The map label for this gene is rihA [H]

Identifier: 86607985

GI number: 86607985

Start: 504359

End: 505294

Strand: Direct

Name: rihA [H]

Synonym: CYB_0491

Alternate gene names: 86607985

Gene position: 504359-505294 (Clockwise)

Preceding gene: 86607977

Following gene: 86607986

Centisome position: 16.55

GC content: 61.97

Gene sequence:

>936_bases
ATGGTGCCCCTGATCATCGACTGTGACCCTGGCCAAGACGATGCGGTGGCTCTGCTGCTGGCTATGGCGTCGCCAGAAGA
GCTGCAATTGCTGGGCATTACCACGGTGGCCGGCAATGTCTCCTTGGACAAAACCAGCCGCAATGCTCGACAGATCTGTG
AATTGGCCGGTCAGCCGCAGATGGGGGTGTATGCCGGCTGCCCTCGACCTCTCTTGCGCCCTCTGGAAACCGCCGAGCAG
GTGCATGGCAAAACCGGGATCGACGGAGCGGATCTGCCGGAGCCCCAGATGCCTTTGGGATCCCAGCATGCGGTGGAGTA
TCTGATTGAAACCCTGATGGCTGCCCCTGAGCCGGTTACCTTGGCTTTGCTGGGCCCGATGACCAACTTGGCAGTGGCCC
TGGTGCAGCAGCCTCGGATTGTGGAGCGCATCCAGCGGCTGGTGTTTATGGGGGGATCCGCCTTTGAGGGGAATGTTACC
CCGGCAGCCGAGTTCAACATCCTCACCGATCCCCACGCCGCCCAGATCGTGCTCAGCGCCGGGATCCCGGAGGTGGTGAT
GCTGGGCTTGCACGTCACCCAACAGGTCCTCAGCACGCCGGAGCGGATTGAGCGCATCCGCGCCCTGGGCACCCGCGTTG
GGCAGACGGTGGCCAATATGCTCACCTTTTACGGTAAGGTCGACGTAGAACGCTACGGGCTACCGGGAGGGCCATTGCAC
GACCCTTGTGTGATTGCCTATCTGTTACAGCCGCAGTTGTTCGAGCTCAAGCCCTGCTACGTCGAGGTGGAAACGGCTAG
CCCCTTGAACCTGGGCCGGACGGTGGTGGATCGCTGGGGATCCAGTGGTCGCCTCGCCAATGTTCAGGTGGCTTTTGGGG
TGGATGCCCAGGGGTTTTACCACCTGCTCACCGAGCGCCTGGGCCGTTACCGATAA

Upstream 100 bases:

>100_bases
TCCTATCTTAAATCTGACCGGGATCCGCGCTCACCGGCCAGAGGAAAAGAGCGGGAGAAAAACCTAAACTAGGGGTGAGT
GAGCTGCTGCCGAGATCCCT

Downstream 100 bases:

>100_bases
TTCTGATAGTTCTGATCCTTGAGGCCAGATCCGAAGGTGGAGAATCCCAGCGCCGTCCAACGTGCCAAGCTGGAGTGGGT
GAACCTGCCCACCGCCGTCA

Product: inosine-uridine preferring nucleoside hydrolase family protein

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MVPLIIDCDPGQDDAVALLLAMASPEELQLLGITTVAGNVSLDKTSRNARQICELAGQPQMGVYAGCPRPLLRPLETAEQ
VHGKTGIDGADLPEPQMPLGSQHAVEYLIETLMAAPEPVTLALLGPMTNLAVALVQQPRIVERIQRLVFMGGSAFEGNVT
PAAEFNILTDPHAAQIVLSAGIPEVVMLGLHVTQQVLSTPERIERIRALGTRVGQTVANMLTFYGKVDVERYGLPGGPLH
DPCVIAYLLQPQLFELKPCYVEVETASPLNLGRTVVDRWGSSGRLANVQVAFGVDAQGFYHLLTERLGRYR

Sequences:

>Translated_311_residues
MVPLIIDCDPGQDDAVALLLAMASPEELQLLGITTVAGNVSLDKTSRNARQICELAGQPQMGVYAGCPRPLLRPLETAEQ
VHGKTGIDGADLPEPQMPLGSQHAVEYLIETLMAAPEPVTLALLGPMTNLAVALVQQPRIVERIQRLVFMGGSAFEGNVT
PAAEFNILTDPHAAQIVLSAGIPEVVMLGLHVTQQVLSTPERIERIRALGTRVGQTVANMLTFYGKVDVERYGLPGGPLH
DPCVIAYLLQPQLFELKPCYVEVETASPLNLGRTVVDRWGSSGRLANVQVAFGVDAQGFYHLLTERLGRYR
>Mature_311_residues
MVPLIIDCDPGQDDAVALLLAMASPEELQLLGITTVAGNVSLDKTSRNARQICELAGQPQMGVYAGCPRPLLRPLETAEQ
VHGKTGIDGADLPEPQMPLGSQHAVEYLIETLMAAPEPVTLALLGPMTNLAVALVQQPRIVERIQRLVFMGGSAFEGNVT
PAAEFNILTDPHAAQIVLSAGIPEVVMLGLHVTQQVLSTPERIERIRALGTRVGQTVANMLTFYGKVDVERYGLPGGPLH
DPCVIAYLLQPQLFELKPCYVEVETASPLNLGRTVVDRWGSSGRLANVQVAFGVDAQGFYHLLTERLGRYR

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=310, Percent_Identity=45.4838709677419, Blast_Score=253, Evalue=8e-69,
Organism=Escherichia coli, GI1788486, Length=307, Percent_Identity=42.3452768729642, Blast_Score=250, Evalue=7e-68,
Organism=Escherichia coli, GI1786213, Length=309, Percent_Identity=44.6601941747573, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI32565474, Length=190, Percent_Identity=33.6842105263158, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17565698, Length=188, Percent_Identity=34.5744680851064, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320608, Length=262, Percent_Identity=30.1526717557252, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24583915, Length=199, Percent_Identity=30.6532663316583, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24641839, Length=198, Percent_Identity=31.8181818181818, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24641837, Length=324, Percent_Identity=26.5432098765432, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 33572; Mature: 33572

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPLIIDCDPGQDDAVALLLAMASPEELQLLGITTVAGNVSLDKTSRNARQICELAGQPQ
CCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCEECCCCHHHHHHHHHCCCCC
MGVYAGCPRPLLRPLETAEQVHGKTGIDGADLPEPQMPLGSQHAVEYLIETLMAAPEPVT
CCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LALLGPMTNLAVALVQQPRIVERIQRLVFMGGSAFEGNVTPAAEFNILTDPHAAQIVLSA
EEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHEEEEEC
GIPEVVMLGLHVTQQVLSTPERIERIRALGTRVGQTVANMLTFYGKVDVERYGLPGGPLH
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
DPCVIAYLLQPQLFELKPCYVEVETASPLNLGRTVVDRWGSSGRLANVQVAFGVDAQGFY
CHHHHHHHHCCCCEECCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCHHHH
HLLTERLGRYR
HHHHHHHHCCC
>Mature Secondary Structure
MVPLIIDCDPGQDDAVALLLAMASPEELQLLGITTVAGNVSLDKTSRNARQICELAGQPQ
CCCEEEECCCCCCHHHHHHHHHCCCCCEEEEEEEEEECCCEECCCCHHHHHHHHHCCCCC
MGVYAGCPRPLLRPLETAEQVHGKTGIDGADLPEPQMPLGSQHAVEYLIETLMAAPEPVT
CCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEE
LALLGPMTNLAVALVQQPRIVERIQRLVFMGGSAFEGNVTPAAEFNILTDPHAAQIVLSA
EEEECCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCHHEEEEEC
GIPEVVMLGLHVTQQVLSTPERIERIRALGTRVGQTVANMLTFYGKVDVERYGLPGGPLH
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCC
DPCVIAYLLQPQLFELKPCYVEVETASPLNLGRTVVDRWGSSGRLANVQVAFGVDAQGFY
CHHHHHHHHCCCCEECCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCHHHH
HLLTERLGRYR
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA