Click here to switch to the map view.

The map label for this gene is ilvE [H]

Identifier: 86607973

GI number: 86607973

Start: 490117

End: 491037

Strand: Reverse

Name: ilvE [H]

Synonym: CYB_0479

Alternate gene names: 86607973

Gene position: 491037-490117 (Counterclockwise)

Preceding gene: 86607976

Following gene: 86607972

Centisome position: 16.12

GC content: 57.65

Gene sequence:

>921_bases
ATGCCAGATTTTCTGCCCTACGCCTACTTTCAGGGCCAGATTGTCCCTTTTGCCGAGGCGAAGATCTCCATTGCCACCCA
TGCTCTGCACTACGGGACAGCGGCGTTTGGCGGCTTGCGCGGGATCCCAGACCCGCAAAACCCGGATCAAGTTCTGGTGT
TCCGTCTGGATCGCCATTGCCGACGACTGAGTCAAAGCGCCCGCCTGCTCTGTTTTGATCTCCCTGCTGAACGGATTGAG
CAGGTGATCGTCGAGCTGATCCGAAAAAACCGTCCCTCCACGTCTTTCTACATCCGCCCCTTGGTGTACACCTCCGACCT
AGGCATCTCGCCGCGGCTGCACAACATTGAGAAAGACTTTTTCGTGTACGGCTTGGAACTGGGGGACTACCTCTCGCCAG
AGGGAGTCAGTTGCCGCATCAGCTCTTGGGCCCGGCAAGAGGATCGCAGCCTACCATTGCGGGGCAAGATCACCGGCGCC
TACATCACTTCCTCTTTGGCCAAAACCGAGGCGGCGCAGGCTGGCTATGACGAGGCCATTTTGATGAACTCCCAGGGCAA
AGTGAGCGAAGCCTCGGGCATGAACATCTTCATCGTGCGCAATGGCGTGCTGATTACCCCCAGCTTCGACCAAGATATCT
TGGAAGGGATCACCCGCGACAGTGTCCTCACCCTGGCCCGTGATCTGGGGATCCCTACCCAAGAACGGCCTGTCGATAAG
TCGGAGCTGTTGATTGCCGATGAAGCTTTCCTGACTGGCACTGCTGCCAAGATTACGCCCATCCGCCAGATCGAGCAGTA
CGTCCTGCCCCCCTCCCGCCCCATCACCGAGCAGTTGCGGCAAAAGCTAACGGCAATCACCGAAAATCGGGATCCCGTCT
ATGCCCATTGGGTGAAGGTGATCCCTCTGAAAGAAAGCTAG

Upstream 100 bases:

>100_bases
ACTACAGGCAGGGAAGTCATGCTGACGTTGGAGCTCCCGGCACTGCACGAATCGGTGATAATATTCCTACCTCTCATTCC
TACCCCGGTGGGTGCCGACC

Downstream 100 bases:

>100_bases
TCCCTAGTCGAGGGGAGAAGCGTCATTATTAAACTGAGCACTCTAGATGGGTAGAGGGGGATCCCTTGCACGAAATTTCC
ATTACCTGGGCCGACTACCA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPDFLPYAYFQGQIVPFAEAKISIATHALHYGTAAFGGLRGIPDPQNPDQVLVFRLDRHCRRLSQSARLLCFDLPAERIE
QVIVELIRKNRPSTSFYIRPLVYTSDLGISPRLHNIEKDFFVYGLELGDYLSPEGVSCRISSWARQEDRSLPLRGKITGA
YITSSLAKTEAAQAGYDEAILMNSQGKVSEASGMNIFIVRNGVLITPSFDQDILEGITRDSVLTLARDLGIPTQERPVDK
SELLIADEAFLTGTAAKITPIRQIEQYVLPPSRPITEQLRQKLTAITENRDPVYAHWVKVIPLKES

Sequences:

>Translated_306_residues
MPDFLPYAYFQGQIVPFAEAKISIATHALHYGTAAFGGLRGIPDPQNPDQVLVFRLDRHCRRLSQSARLLCFDLPAERIE
QVIVELIRKNRPSTSFYIRPLVYTSDLGISPRLHNIEKDFFVYGLELGDYLSPEGVSCRISSWARQEDRSLPLRGKITGA
YITSSLAKTEAAQAGYDEAILMNSQGKVSEASGMNIFIVRNGVLITPSFDQDILEGITRDSVLTLARDLGIPTQERPVDK
SELLIADEAFLTGTAAKITPIRQIEQYVLPPSRPITEQLRQKLTAITENRDPVYAHWVKVIPLKES
>Mature_305_residues
PDFLPYAYFQGQIVPFAEAKISIATHALHYGTAAFGGLRGIPDPQNPDQVLVFRLDRHCRRLSQSARLLCFDLPAERIEQ
VIVELIRKNRPSTSFYIRPLVYTSDLGISPRLHNIEKDFFVYGLELGDYLSPEGVSCRISSWARQEDRSLPLRGKITGAY
ITSSLAKTEAAQAGYDEAILMNSQGKVSEASGMNIFIVRNGVLITPSFDQDILEGITRDSVLTLARDLGIPTQERPVDKS
ELLIADEAFLTGTAAKITPIRQIEQYVLPPSRPITEQLRQKLTAITENRDPVYAHWVKVIPLKES

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=298, Percent_Identity=35.9060402684564, Blast_Score=192, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6322002, Length=338, Percent_Identity=27.810650887574, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322608, Length=330, Percent_Identity=26.0606060606061, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34242; Mature: 34110

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFLPYAYFQGQIVPFAEAKISIATHALHYGTAAFGGLRGIPDPQNPDQVLVFRLDRHC
CCCCCCCHHHCCCEEEHHHCEEEHHHHHHHHCHHHHCCCCCCCCCCCCCEEEEEHHHHHH
RRLSQSARLLCFDLPAERIEQVIVELIRKNRPSTSFYIRPLVYTSDLGISPRLHNIEKDF
HHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCHHCCCCCE
FVYGLELGDYLSPEGVSCRISSWARQEDRSLPLRGKITGAYITSSLAKTEAAQAGYDEAI
EEEEEECCCCCCCCCCEEEEHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEE
LMNSQGKVSEASGMNIFIVRNGVLITPSFDQDILEGITRDSVLTLARDLGIPTQERPVDK
EECCCCCCCCCCCCEEEEEECCEEEECCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCH
SELLIADEAFLTGTAAKITPIRQIEQYVLPPSRPITEQLRQKLTAITENRDPVYAHWVKV
HHEEEECCHHCCCCCHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEE
IPLKES
EEECCC
>Mature Secondary Structure 
PDFLPYAYFQGQIVPFAEAKISIATHALHYGTAAFGGLRGIPDPQNPDQVLVFRLDRHC
CCCCCCHHHCCCEEEHHHCEEEHHHHHHHHCHHHHCCCCCCCCCCCCCEEEEEHHHHHH
RRLSQSARLLCFDLPAERIEQVIVELIRKNRPSTSFYIRPLVYTSDLGISPRLHNIEKDF
HHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCHHCCCCCE
FVYGLELGDYLSPEGVSCRISSWARQEDRSLPLRGKITGAYITSSLAKTEAAQAGYDEAI
EEEEEECCCCCCCCCCEEEEHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCEE
LMNSQGKVSEASGMNIFIVRNGVLITPSFDQDILEGITRDSVLTLARDLGIPTQERPVDK
EECCCCCCCCCCCCEEEEEECCEEEECCCCHHHHHHCCHHHHHHHHHHCCCCCCCCCCCH
SELLIADEAFLTGTAAKITPIRQIEQYVLPPSRPITEQLRQKLTAITENRDPVYAHWVKV
HHEEEECCHHCCCCCHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEE
IPLKES
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]