Click here to switch to the map view.

The map label for this gene is cbiG/cobJ [H]

Identifier: 86607894

GI number: 86607894

Start: 410818

End: 412713

Strand: Direct

Name: cbiG/cobJ [H]

Synonym: CYB_0397

Alternate gene names: 86607894

Gene position: 410818-412713 (Clockwise)

Preceding gene: 86607889

Following gene: 86607898

Centisome position: 13.48

GC content: 65.45

Gene sequence:

>1896_bases
ATGAAGCTTGCTCTGTTTGCCCCCAGCCCGCGCGCCCGCCGGTTGGCCAACTCCCTGATCCCCCTCCTAGGCAAGGAGGT
CATCTGCTGGAGCAAAGCTGAACCGCAGGGGCTGCCCTCCCCCTGGCAAGCCTACGGGGATCCCAAGGATCCCACAGCGC
CAGGCTCCCTAGCAGAAGCCGTTGCCCAAACCTGGCCCCAGGTGCAGGGCTGTCTCTTCATCTTGGCCGCAGGGTCGGTG
GTGCGGCTCATTGCCCCCCTCTTGCAGGACAAGCAGCGGGATCCAGCGGTGGTGGTGGTGGACGAAGCCGGTCGCTGGGC
TATTAGCCTGTGCGGGGGACATGTGGGGGGGGCCGATGCCCTGGCGCGGCAGGTGGCCGCTACCTTGGGAGCCCAGCCGG
TTCTCACCTCTGCTTCCGAGGGGCTGGGGATCCCGCCTTTGGATTTGCTGGGGGATCCCTACGGCTGGCGGCGGGGCGCT
GGAGATTGGAACGGGGTGGCAGCCGCCTTGACGCGGGGAGAGCCGGTGGCCGTTCACCAGACCTGCGGCACGCGGCTTTG
GCGACAAGCTTGGCCGGCAGATCATCCCCTAGTGGCCTCCTCTCCTCCCCATCCCGTTGCCCAGATCTGGATTGGGGAAT
ACCTCTCCCCTCCCACGGAGATCCCGACAGTGCACTGGCATCCGCGGGTGCTGTGGGTGGGGGTGGGCTGCGAGCGGGGC
ACCACCCGCGCTTGGCTAGAGGGATCCCTGCGCCGCCTGCTGCAGGCCCAGGGGCTGGCGTTCGAGGCCATTGCCGGCCT
GGCCACCCTAGAGCTCAAGCAAGACGAGCCAGGTCTTCTGGAGCTGGCCCAAGCCTATGGCTGGCCCATTGAGTTTTTCT
CGGCAGAGGCTTTGGCTGGGATCCCAGTGCCCCACCCTTCGGAGGCGGTGCGGCAGGCGGTGGGCACTCCCTCGGTAGCG
GAGGCGGCTGCTCTTTTGGCGGCTCAGATCTGGGGAAGAGGTGACCGAGCTTTTGAGAGGCCCTCTTGCGTTGCGGAGGG
GAAGGCAACTCTTCTCTGCCCCAAGCAGGTCTTCACGTCAGAAACCGAAGGGGCCTGCACCCTGGCCATTGCCCGCGCGG
CGCAGGAGTATTCCCCTCGCAGCGGCCACCTCTGGCTCATCGGCACTGGTCCTGGGGATCTGGGTCAATTGACTTTGGCT
GCCCGTATGGCTTTAACTCAGGCCGATGTGGTTGTTGGCTACCAGCTTTATCTGGATCTGGTGGATCCCCTTTTGCACCC
CGGCCAAATCCGGGAGGCCTCTCCCATTACCCAAGAGCTGCAGCGAGCCGAACGGGCGATTGCTTGGGCGCAGCAGGGGC
TGAAGGTGGCGGTGATTTCTTCGGGGGATGCCGGCATTTACGGCATGGCGGGCTTGGTGCTGGAGTGCTTGGTCCGCTCT
GGCTGGGATGGGATCCGCCCTTCTCTGGAGGTGCTGCCGGGGATCTCTGCCTTCCAGGCGGCTGCTGCCCGGCTGGGGGC
TCCGCTGATGCACGACTTCTGCGCCATTTCCCTCTCGGATCTGCTTACCCCTTGGCCGGTGATTGCAAAACGCCTGCAGG
CGGCTGCAGCTGCCGATTTTGTGGTGGCCCTTTACAACCCCCGCTCCCGGCAGCGGGTGGATCCCTTCCTCAAGGCTCTG
CAGATCTTTTTGCAGGCCCGGCCCGCCCATACCCCTGTGGCTTTGGCCCGCAACCTCTACCGCCCTGGCGAAACTCTCCG
CCTTACCACCCTGGGGGAGCTGGATCCAGAGGAGGTGGACATGCTCACCCTGGTTCTTATCGGCAACTCTCAGACCTTTC
GCCATGGGGAGCGGCTGATTACCCCGCGGGGCTACCGCTTGGGCGAGCCCACCTGA

Upstream 100 bases:

>100_bases
CATCAGTCCAGGTGGGATGACCATCGGCACTTGTCCCTTCGCGGGTGGCTCTATGTCCAGTATAGGATCCGGGGAGAATC
CTGGCCTTGGCCCACGCTCG

Downstream 100 bases:

>100_bases
CTCCTGGGCTCCAGACTCAAAACACCCTCAGATGCCGGGGATCCAACTGTAGCCACCAGGGGGATCCCCAAGTCTCCACC
TCTCGCCAGCGCTCCCGCCA

Product: cbiG protein/precorrin-3B C17-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 4

Alternate protein names: Cobalt-precorrin-3 methylase; Cobalt-precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 631; Mature: 631

Protein sequence:

>631_residues
MKLALFAPSPRARRLANSLIPLLGKEVICWSKAEPQGLPSPWQAYGDPKDPTAPGSLAEAVAQTWPQVQGCLFILAAGSV
VRLIAPLLQDKQRDPAVVVVDEAGRWAISLCGGHVGGADALARQVAATLGAQPVLTSASEGLGIPPLDLLGDPYGWRRGA
GDWNGVAAALTRGEPVAVHQTCGTRLWRQAWPADHPLVASSPPHPVAQIWIGEYLSPPTEIPTVHWHPRVLWVGVGCERG
TTRAWLEGSLRRLLQAQGLAFEAIAGLATLELKQDEPGLLELAQAYGWPIEFFSAEALAGIPVPHPSEAVRQAVGTPSVA
EAAALLAAQIWGRGDRAFERPSCVAEGKATLLCPKQVFTSETEGACTLAIARAAQEYSPRSGHLWLIGTGPGDLGQLTLA
ARMALTQADVVVGYQLYLDLVDPLLHPGQIREASPITQELQRAERAIAWAQQGLKVAVISSGDAGIYGMAGLVLECLVRS
GWDGIRPSLEVLPGISAFQAAAARLGAPLMHDFCAISLSDLLTPWPVIAKRLQAAAAADFVVALYNPRSRQRVDPFLKAL
QIFLQARPAHTPVALARNLYRPGETLRLTTLGELDPEEVDMLTLVLIGNSQTFRHGERLITPRGYRLGEPT

Sequences:

>Translated_631_residues
MKLALFAPSPRARRLANSLIPLLGKEVICWSKAEPQGLPSPWQAYGDPKDPTAPGSLAEAVAQTWPQVQGCLFILAAGSV
VRLIAPLLQDKQRDPAVVVVDEAGRWAISLCGGHVGGADALARQVAATLGAQPVLTSASEGLGIPPLDLLGDPYGWRRGA
GDWNGVAAALTRGEPVAVHQTCGTRLWRQAWPADHPLVASSPPHPVAQIWIGEYLSPPTEIPTVHWHPRVLWVGVGCERG
TTRAWLEGSLRRLLQAQGLAFEAIAGLATLELKQDEPGLLELAQAYGWPIEFFSAEALAGIPVPHPSEAVRQAVGTPSVA
EAAALLAAQIWGRGDRAFERPSCVAEGKATLLCPKQVFTSETEGACTLAIARAAQEYSPRSGHLWLIGTGPGDLGQLTLA
ARMALTQADVVVGYQLYLDLVDPLLHPGQIREASPITQELQRAERAIAWAQQGLKVAVISSGDAGIYGMAGLVLECLVRS
GWDGIRPSLEVLPGISAFQAAAARLGAPLMHDFCAISLSDLLTPWPVIAKRLQAAAAADFVVALYNPRSRQRVDPFLKAL
QIFLQARPAHTPVALARNLYRPGETLRLTTLGELDPEEVDMLTLVLIGNSQTFRHGERLITPRGYRLGEPT
>Mature_631_residues
MKLALFAPSPRARRLANSLIPLLGKEVICWSKAEPQGLPSPWQAYGDPKDPTAPGSLAEAVAQTWPQVQGCLFILAAGSV
VRLIAPLLQDKQRDPAVVVVDEAGRWAISLCGGHVGGADALARQVAATLGAQPVLTSASEGLGIPPLDLLGDPYGWRRGA
GDWNGVAAALTRGEPVAVHQTCGTRLWRQAWPADHPLVASSPPHPVAQIWIGEYLSPPTEIPTVHWHPRVLWVGVGCERG
TTRAWLEGSLRRLLQAQGLAFEAIAGLATLELKQDEPGLLELAQAYGWPIEFFSAEALAGIPVPHPSEAVRQAVGTPSVA
EAAALLAAQIWGRGDRAFERPSCVAEGKATLLCPKQVFTSETEGACTLAIARAAQEYSPRSGHLWLIGTGPGDLGQLTLA
ARMALTQADVVVGYQLYLDLVDPLLHPGQIREASPITQELQRAERAIAWAQQGLKVAVISSGDAGIYGMAGLVLECLVRS
GWDGIRPSLEVLPGISAFQAAAARLGAPLMHDFCAISLSDLLTPWPVIAKRLQAAAAADFVVALYNPRSRQRVDPFLKAL
QIFLQARPAHTPVALARNLYRPGETLRLTTLGELDPEEVDMLTLVLIGNSQTFRHGERLITPRGYRLGEPT

Specific function: Methyltransferase that catalyzes the methylation of C-17 in cobalt-precorrin-3B to form cobalt-precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.131

Molecular weight: Translated: 67459; Mature: 67459

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLALFAPSPRARRLANSLIPLLGKEVICWSKAEPQGLPSPWQAYGDPKDPTAPGSLAEA
CEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHCCCCCCCCCCHHHHHH
VAQTWPQVQGCLFILAAGSVVRLIAPLLQDKQRDPAVVVVDEAGRWAISLCGGHVGGADA
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCHHH
LARQVAATLGAQPVLTSASEGLGIPPLDLLGDPYGWRRGAGDWNGVAAALTRGEPVAVHQ
HHHHHHHHHCCCCHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCEEEHH
TCGTRLWRQAWPADHPLVASSPPHPVAQIWIGEYLSPPTEIPTVHWHPRVLWVGVGCERG
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCEEEEEEECCCCC
TTRAWLEGSLRRLLQAQGLAFEAIAGLATLELKQDEPGLLELAQAYGWPIEFFSAEALAG
CCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEECCCHHHCC
IPVPHPSEAVRQAVGTPSVAEAAALLAAQIWGRGDRAFERPSCVAEGKATLLCPKQVFTS
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEECHHHHCC
ETEGACTLAIARAAQEYSPRSGHLWLIGTGPGDLGQLTLAARMALTQADVVVGYQLYLDL
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDPLLHPGQIREASPITQELQRAERAIAWAQQGLKVAVISSGDAGIYGMAGLVLECLVRS
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHC
GWDGIRPSLEVLPGISAFQAAAARLGAPLMHDFCAISLSDLLTPWPVIAKRLQAAAAADF
CCCCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCE
VVALYNPRSRQRVDPFLKALQIFLQARPAHTPVALARNLYRPGETLRLTTLGELDPEEVD
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHC
MLTLVLIGNSQTFRHGERLITPRGYRLGEPT
EEEEEEECCCCHHHCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MKLALFAPSPRARRLANSLIPLLGKEVICWSKAEPQGLPSPWQAYGDPKDPTAPGSLAEA
CEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHCCCCCCCCCCHHHHHH
VAQTWPQVQGCLFILAAGSVVRLIAPLLQDKQRDPAVVVVDEAGRWAISLCGGHVGGADA
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCHHH
LARQVAATLGAQPVLTSASEGLGIPPLDLLGDPYGWRRGAGDWNGVAAALTRGEPVAVHQ
HHHHHHHHHCCCCHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHCCCCCEEEHH
TCGTRLWRQAWPADHPLVASSPPHPVAQIWIGEYLSPPTEIPTVHWHPRVLWVGVGCERG
HHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCEEEEEEECCCCC
TTRAWLEGSLRRLLQAQGLAFEAIAGLATLELKQDEPGLLELAQAYGWPIEFFSAEALAG
CCHHHHHHHHHHHHHHCCCHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCEECCCHHHCC
IPVPHPSEAVRQAVGTPSVAEAAALLAAQIWGRGDRAFERPSCVAEGKATLLCPKQVFTS
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEECHHHHCC
ETEGACTLAIARAAQEYSPRSGHLWLIGTGPGDLGQLTLAARMALTQADVVVGYQLYLDL
CCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDPLLHPGQIREASPITQELQRAERAIAWAQQGLKVAVISSGDAGIYGMAGLVLECLVRS
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHC
GWDGIRPSLEVLPGISAFQAAAARLGAPLMHDFCAISLSDLLTPWPVIAKRLQAAAAADF
CCCCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCE
VVALYNPRSRQRVDPFLKALQIFLQARPAHTPVALARNLYRPGETLRLTTLGELDPEEVD
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHC
MLTLVLIGNSQTFRHGERLITPRGYRLGEPT
EEEEEEECCCCHHHCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 3B

Specific reaction: S-adenosyl-L-methionine + precorrin-3B = S-adenosyl-L-homocysteine + precorrin-4

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]