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The map label for this gene is yfhM [H]

Identifier: 86607846

GI number: 86607846

Start: 367081

End: 367986

Strand: Reverse

Name: yfhM [H]

Synonym: CYB_0349

Alternate gene names: 86607846

Gene position: 367986-367081 (Counterclockwise)

Preceding gene: 86607848

Following gene: 86607845

Centisome position: 12.08

GC content: 58.39

Gene sequence:

>906_bases
ATGGCAGAAGCAGGGATCCCCCGCCTTCCTCCTGAGGTAGCCGCCAAGATGGGAACCTGGCAGCACGGGTATGCCCTTAC
CAATGGCATTCAACTGCACTACGTCACCCAAGGGGAAGGGGAGCTGGCGATCTTGTTGCACGGTTTCCCGGAGTTTTGGT
ACTCCTGGCGGCATCAGATTCCGGTGTTGGCGCAGCGGTTTCGCGTGGTGGCACCCGATATGCGCGGCTACAACGATTCC
GACAAACCGGATCACGGCTATGACCTGGATACCCTTACCGAGGATATTCGCGGCTTGCTGAGCCACTTTGGGGCCCGAAG
AGCGGTGGTCGTAGCCCACGATTGGGGGGGAGCCATTGCCTGGCACTGGGCGCAATTTTTTCCTGAGGAGATTCGCAAGC
TGGCCGTCCTCAATTCTCCCCATCCGGCCTGCTTTCGCCGTGAATTCTTGAGCAACTTGGATCAACTGCGCCGCAGTTGG
TACCTGTTCTTTTTTCAATTGCCTTGGCTGCCAGAGTGGGTGCTGCAGTGGGATTTGGGAGATTGGGTGCGCCGCATTTT
CCAGGAAACCTCGGTGCGCAAAAGCGCCTTCACCCGCCACGACCTCAAGCTCTACCAAGAGGCCCTCTCCAAACCCAAGG
TTCTCACCTCTGCCCTCAACTACTACCGCCAGCTGTTTAACCTGCCCACCTTGCAGAACCTGTTTTTGCAGCCCATGCGG
CAGATCCTCGCCCCCACCCTGCTGATTTGGGGAGAAGAAGACTTTGCCCTCAGCCGCGAGCTAACCGAGGGGATGGATCC
CTTTTTCCCCAACGGCCTGCGCAAGGAGTACATCCCCGAATGCGGCCATTGGGCGCAGCAGGAAGCTCCGCAAACGGTCA
ATCGGCTGCTGATGGAGTTTCTCTAG

Upstream 100 bases:

>100_bases
AGGATGCCTCTCAAAAAGCCTGCGCCGGCAAAGGGAAGACCGGCGACAAAGGTTAGTATGGGTGGATACCAAATCATGGG
TTAGCTCGAGAGCAGACGAC

Downstream 100 bases:

>100_bases
CGGGCCGGGCTTGGGATCCGCTCTTTACCATCTTCAAGGGTTGACCCTAGAATAATCCACTGAGTTTATGGGAGAACCGG
CATGGCCCTGGAGTATCCAT

Product: alpha/beta fold family hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MAEAGIPRLPPEVAAKMGTWQHGYALTNGIQLHYVTQGEGELAILLHGFPEFWYSWRHQIPVLAQRFRVVAPDMRGYNDS
DKPDHGYDLDTLTEDIRGLLSHFGARRAVVVAHDWGGAIAWHWAQFFPEEIRKLAVLNSPHPACFRREFLSNLDQLRRSW
YLFFFQLPWLPEWVLQWDLGDWVRRIFQETSVRKSAFTRHDLKLYQEALSKPKVLTSALNYYRQLFNLPTLQNLFLQPMR
QILAPTLLIWGEEDFALSRELTEGMDPFFPNGLRKEYIPECGHWAQQEAPQTVNRLLMEFL

Sequences:

>Translated_301_residues
MAEAGIPRLPPEVAAKMGTWQHGYALTNGIQLHYVTQGEGELAILLHGFPEFWYSWRHQIPVLAQRFRVVAPDMRGYNDS
DKPDHGYDLDTLTEDIRGLLSHFGARRAVVVAHDWGGAIAWHWAQFFPEEIRKLAVLNSPHPACFRREFLSNLDQLRRSW
YLFFFQLPWLPEWVLQWDLGDWVRRIFQETSVRKSAFTRHDLKLYQEALSKPKVLTSALNYYRQLFNLPTLQNLFLQPMR
QILAPTLLIWGEEDFALSRELTEGMDPFFPNGLRKEYIPECGHWAQQEAPQTVNRLLMEFL
>Mature_300_residues
AEAGIPRLPPEVAAKMGTWQHGYALTNGIQLHYVTQGEGELAILLHGFPEFWYSWRHQIPVLAQRFRVVAPDMRGYNDSD
KPDHGYDLDTLTEDIRGLLSHFGARRAVVVAHDWGGAIAWHWAQFFPEEIRKLAVLNSPHPACFRREFLSNLDQLRRSWY
LFFFQLPWLPEWVLQWDLGDWVRRIFQETSVRKSAFTRHDLKLYQEALSKPKVLTSALNYYRQLFNLPTLQNLFLQPMRQ
ILAPTLLIWGEEDFALSRELTEGMDPFFPNGLRKEYIPECGHWAQQEAPQTVNRLLMEFL

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=302, Percent_Identity=37.4172185430464, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI218777840, Length=298, Percent_Identity=35.2348993288591, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI13376164, Length=298, Percent_Identity=35.2348993288591, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI27597073, Length=312, Percent_Identity=31.7307692307692, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17554012, Length=290, Percent_Identity=37.5862068965517, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17562298, Length=293, Percent_Identity=34.4709897610922, Blast_Score=187, Evalue=8e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 35292; Mature: 35161

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAGIPRLPPEVAAKMGTWQHGYALTNGIQLHYVTQGEGELAILLHGFPEFWYSWRHQI
CCCCCCCCCCHHHHHHHCCCCCCCEEECCEEEEEEECCCCCEEEEEECCHHHHHHHHCCH
PVLAQRFRVVAPDMRGYNDSDKPDHGYDLDTLTEDIRGLLSHFGARRAVVVAHDWGGAIA
HHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHH
WHWAQFFPEEIRKLAVLNSPHPACFRREFLSNLDQLRRSWYLFFFQLPWLPEWVLQWDLG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHH
DWVRRIFQETSVRKSAFTRHDLKLYQEALSKPKVLTSALNYYRQLFNLPTLQNLFLQPMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QILAPTLLIWGEEDFALSRELTEGMDPFFPNGLRKEYIPECGHWAQQEAPQTVNRLLMEF
HHHCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHH
L
C
>Mature Secondary Structure 
AEAGIPRLPPEVAAKMGTWQHGYALTNGIQLHYVTQGEGELAILLHGFPEFWYSWRHQI
CCCCCCCCCHHHHHHHCCCCCCCEEECCEEEEEEECCCCCEEEEEECCHHHHHHHHCCH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C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]