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The map label for this gene is gcvH [H]

Identifier: 86607845

GI number: 86607845

Start: 366610

End: 366999

Strand: Reverse

Name: gcvH [H]

Synonym: CYB_0348

Alternate gene names: 86607845

Gene position: 366999-366610 (Counterclockwise)

Preceding gene: 86607846

Following gene: 86607843

Centisome position: 12.05

GC content: 55.13

Gene sequence:

>390_bases
ATGGCCCTGGAGTATCCATCCCATCTGCGCTACGTGGACACCCACGAGTACATCCATGTTGAGGATGATATTGCGGTGAT
TGGGATCACGGCTTATGCCGTCGATCAACTGGGGGATATCGTTTTCGTGGGCTTGCCCGAAGAAGGGACAGAGATTGAAA
AAGGGGAAAGCTTTGGCTCGGTGGAATCGGTCAAAGCCGTCGAAGATCTCTATGCCCCTCTTTCTGGAGAGGTGGTGGCC
GTGAATACAGCGGTGGTGGAGAGCCCCGAAAGCCTTGCCGACGATCCCTATGGCGATGGCTGGCTGATCAAGGTGCGCAT
TGCCAACCCCGAAGACTTGGAAGACACCATGAGTGCCGAAGCCTATGCCAGCTTGGTAGAGGGATCCTAA

Upstream 100 bases:

>100_bases
GCTGATGGAGTTTCTCTAGCGGGCCGGGCTTGGGATCCGCTCTTTACCATCTTCAAGGGTTGACCCTAGAATAATCCACT
GAGTTTATGGGAGAACCGGC

Downstream 100 bases:

>100_bases
ACTCCAGCAGTTGGCCCTGAACTACAGCAAAGTCTCTCGGTTACCCCCCTCAGCTTTGGCTTGGTAGAGGGCCTTGTCGG
CAGCAGCCAGCAGACTTTGT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MALEYPSHLRYVDTHEYIHVEDDIAVIGITAYAVDQLGDIVFVGLPEEGTEIEKGESFGSVESVKAVEDLYAPLSGEVVA
VNTAVVESPESLADDPYGDGWLIKVRIANPEDLEDTMSAEAYASLVEGS

Sequences:

>Translated_129_residues
MALEYPSHLRYVDTHEYIHVEDDIAVIGITAYAVDQLGDIVFVGLPEEGTEIEKGESFGSVESVKAVEDLYAPLSGEVVA
VNTAVVESPESLADDPYGDGWLIKVRIANPEDLEDTMSAEAYASLVEGS
>Mature_128_residues
ALEYPSHLRYVDTHEYIHVEDDIAVIGITAYAVDQLGDIVFVGLPEEGTEIEKGESFGSVESVKAVEDLYAPLSGEVVAV
NTAVVESPESLADDPYGDGWLIKVRIANPEDLEDTMSAEAYASLVEGS

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=45.1327433628319, Blast_Score=118, Evalue=2e-27,
Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=44.2477876106195, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1789271, Length=123, Percent_Identity=43.9024390243902, Blast_Score=108, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=44.3478260869565, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=125, Percent_Identity=42.4, Blast_Score=110, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=47.9338842975207, Blast_Score=115, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17865652, Length=121, Percent_Identity=39.6694214876033, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13905; Mature: 13774

Theoretical pI: Translated: 3.65; Mature: 3.65

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALEYPSHLRYVDTHEYIHVEDDIAVIGITAYAVDQLGDIVFVGLPEEGTEIEKGESFGS
CCCCCCCCCEEECCCCEEEECCCEEEEEEHHHHHHCCCCEEEEECCCCCCCCCCCCCCCC
VESVKAVEDLYAPLSGEVVAVNTAVVESPESLADDPYGDGWLIKVRIANPEDLEDTMSAE
HHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHCCCCCCCCEEEEEEECCCCCHHHHHHHH
AYASLVEGS
HHHHHHCCC
>Mature Secondary Structure 
ALEYPSHLRYVDTHEYIHVEDDIAVIGITAYAVDQLGDIVFVGLPEEGTEIEKGESFGS
CCCCCCCCEEECCCCEEEECCCEEEEEEHHHHHHCCCCEEEEECCCCCCCCCCCCCCCC
VESVKAVEDLYAPLSGEVVAVNTAVVESPESLADDPYGDGWLIKVRIANPEDLEDTMSAE
HHHHHHHHHHHCCCCCCEEEEEEEEECCCHHHCCCCCCCCEEEEEEECCCCCHHHHHHHH
AYASLVEGS
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA