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The map label for this gene is gyrB [H]

Identifier: 86607821

GI number: 86607821

Start: 337816

End: 339744

Strand: Reverse

Name: gyrB [H]

Synonym: CYB_0322

Alternate gene names: 86607821

Gene position: 339744-337816 (Counterclockwise)

Preceding gene: 86607822

Following gene: 86607820

Centisome position: 11.15

GC content: 57.44

Gene sequence:

>1929_bases
ATGACGGAAAACTACGGTGCAGAGCAGATCCAAGTTTTGGAAGGCTTGGAGGCTGTGCGCAAGCGGCCTGGCATGTACAT
TGGCTCCACCGGGCCGAAGGGGTTGCACCATTTGGTGTACGAGGTGGTGGACAACTCTGTGGATGAGGCCCTGGCCGGCT
ATTGCAATGATATTCAGATCAGCCTGCATCCCGACGGCTCGGTATCCGTTTTGGACAATGGCCGTGGCATTCCGGTGGAC
ATCCACCCCAAAACCGGTCAGTCGGCCCTGGTTACGGTGCTGACGGTGCTGCACGCGGGGGGCAAGTTTGGCGGTGGCGG
CTACAAAGTCTCCGGTGGGCTGCACGGGGTGGGCGTGTCGGTGGTGAATGCCCTCTCCGAATGGGTGGAGGTGACGGTTT
GGCGAGAGGGCAAGGTTTACTTTCAACGTTTCGAGCGCGGGGCGGTGGTGGGATCCCTGAAATCTGAACCCCAACCCAAG
GGATCCAACCGGCGGGGTACAGAGGTGCGCTTCAAGCCGGATGGGCTGATCTTTCCCGAGCGAGAGTTTGACTATGCCGT
ATTGGCGGCCCGCTTTCGCGAGTTGGCCTACCTCAATGCCGGCTTGAAAATTACCTTTAGCGATCTGCGGCGGGATCCGC
CTCACTGCGAAACCTATCAATATGCAGGCGGCATCAAGGAATATGTAGCCTTTATCAACCACGAAAAAACGCCCATCCAC
TCCGACATCATCTACATCCAAGGGGAGCGGGATGGGGTGGAGGTGGAAGTGGCCCTGCAGTGGTGCTCGGATGTGTACAC
CGACACTTTGATTGGCTTTGCCAACAACATTCGCACCAACGAAGGAGGCACCCACCTAGAGGGCCTGAAAACCTCTCTCA
CCCTCACCATCAACAGCCAAGCCCGCAAACTCAACAAGCTGAAAGATTCCGACAGCAACCTGGCTGGGGAAAATGTGCGC
GAGGGGTTGACGGCCATCGTCTCGGTCAAGGTACCCAACCCCGAGTTTGAGGGGCAAACCAAAACCAAGTTGGGCAACAC
CGAGGTGCGGGGCATTGTGCAAACCCTCGTCTCCGAGGCGCTCACGGAGTACTTGGAGTTCAACCCCAACGTGGCCAGGG
GGATCGTCGATAAGGCCATCCAGTCCTTCAATGCTGCCGAGGCAGCCCGGCGGGCCAGAGAATTGGTGCGGCGCAAATCT
GCCCTGGAATCTTCCACGCTGCCGGGGAAGTTGGCCGATTGCAGTTGCCGCGACCCGGCGGAGTCTGAGGTGTATTTGGT
GGAAGGCGACTCTGCAGGGGGCAGCGCCAAACAAGGTCGGGATCGGCAGTTTCAGGCGGTGTTGCCCTTGCGCGGCAAGA
TCCTCAACATTGAAAAGGCGGATCCCGCCAAGATCTACAAAAACGCCGAAATTCAGGCGATGATCACAGCGCTGGGTTTG
GGAGTTAAAGGCGATGAATTTGATCCCAGCCAACTGCGCTACCACCGCATCATCCTCATGACCGATGCCGATGTGGACGG
TAGCCACATTCGCACCCTGTTGCTGACCTTTTTCTACCGCTATCAAAAGGCGCTTCTGGAACAGGGCTATGTCTATATCG
CCTGTCCGCCGCTCTACAAAATCGAGTGGGGTCGCAACAATGTTCGCTACTGCTACAGCGACGCCGAGAAAGACCAAATC
CTGCGGGGGCTGCCTGCCAACGCCAAGTACACCATCCAACGGTTCAAGGGTCTGGGGGAAATGATGCCTGAACAACTTTG
GGAGACCACCATGAACCCGGCAACGCGCACCCTCAAACAAATCACCATCGAAGATGCGGTGGAGGCGGATCGCATTTTCA
CCATCTTGATGGGAGATGCTGTTGGTCCCCGACGGGAGTTTATTGAGGCCCACAGTGCCCATTTGGATCTGGCGGCTCTC
GATATTTAG

Upstream 100 bases:

>100_bases
ACAGTTTTTTGATGATAAGCTGATTCCTGGCCTTTAAGGTCAATTTTTCTGGGTTTCTGTTGATCTTGGCCGGGAGCCTT
CTTCGGAATTGGAGTTGGGA

Downstream 100 bases:

>100_bases
CGACTCTCGATATTGGGTGGAGAGAACTCTGTCCCGCCTCTTCGGGGCCCACGGCAGGGGGAAAGTGCTCCGCACCGCTG
GCGCGAACGTAACGGTGTTG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MTENYGAEQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVYEVVDNSVDEALAGYCNDIQISLHPDGSVSVLDNGRGIPVD
IHPKTGQSALVTVLTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSEWVEVTVWREGKVYFQRFERGAVVGSLKSEPQPK
GSNRRGTEVRFKPDGLIFPEREFDYAVLAARFRELAYLNAGLKITFSDLRRDPPHCETYQYAGGIKEYVAFINHEKTPIH
SDIIYIQGERDGVEVEVALQWCSDVYTDTLIGFANNIRTNEGGTHLEGLKTSLTLTINSQARKLNKLKDSDSNLAGENVR
EGLTAIVSVKVPNPEFEGQTKTKLGNTEVRGIVQTLVSEALTEYLEFNPNVARGIVDKAIQSFNAAEAARRARELVRRKS
ALESSTLPGKLADCSCRDPAESEVYLVEGDSAGGSAKQGRDRQFQAVLPLRGKILNIEKADPAKIYKNAEIQAMITALGL
GVKGDEFDPSQLRYHRIILMTDADVDGSHIRTLLLTFFYRYQKALLEQGYVYIACPPLYKIEWGRNNVRYCYSDAEKDQI
LRGLPANAKYTIQRFKGLGEMMPEQLWETTMNPATRTLKQITIEDAVEADRIFTILMGDAVGPRREFIEAHSAHLDLAAL
DI

Sequences:

>Translated_642_residues
MTENYGAEQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVYEVVDNSVDEALAGYCNDIQISLHPDGSVSVLDNGRGIPVD
IHPKTGQSALVTVLTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSEWVEVTVWREGKVYFQRFERGAVVGSLKSEPQPK
GSNRRGTEVRFKPDGLIFPEREFDYAVLAARFRELAYLNAGLKITFSDLRRDPPHCETYQYAGGIKEYVAFINHEKTPIH
SDIIYIQGERDGVEVEVALQWCSDVYTDTLIGFANNIRTNEGGTHLEGLKTSLTLTINSQARKLNKLKDSDSNLAGENVR
EGLTAIVSVKVPNPEFEGQTKTKLGNTEVRGIVQTLVSEALTEYLEFNPNVARGIVDKAIQSFNAAEAARRARELVRRKS
ALESSTLPGKLADCSCRDPAESEVYLVEGDSAGGSAKQGRDRQFQAVLPLRGKILNIEKADPAKIYKNAEIQAMITALGL
GVKGDEFDPSQLRYHRIILMTDADVDGSHIRTLLLTFFYRYQKALLEQGYVYIACPPLYKIEWGRNNVRYCYSDAEKDQI
LRGLPANAKYTIQRFKGLGEMMPEQLWETTMNPATRTLKQITIEDAVEADRIFTILMGDAVGPRREFIEAHSAHLDLAAL
DI
>Mature_641_residues
TENYGAEQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVYEVVDNSVDEALAGYCNDIQISLHPDGSVSVLDNGRGIPVDI
HPKTGQSALVTVLTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSEWVEVTVWREGKVYFQRFERGAVVGSLKSEPQPKG
SNRRGTEVRFKPDGLIFPEREFDYAVLAARFRELAYLNAGLKITFSDLRRDPPHCETYQYAGGIKEYVAFINHEKTPIHS
DIIYIQGERDGVEVEVALQWCSDVYTDTLIGFANNIRTNEGGTHLEGLKTSLTLTINSQARKLNKLKDSDSNLAGENVRE
GLTAIVSVKVPNPEFEGQTKTKLGNTEVRGIVQTLVSEALTEYLEFNPNVARGIVDKAIQSFNAAEAARRARELVRRKSA
LESSTLPGKLADCSCRDPAESEVYLVEGDSAGGSAKQGRDRQFQAVLPLRGKILNIEKADPAKIYKNAEIQAMITALGLG
VKGDEFDPSQLRYHRIILMTDADVDGSHIRTLLLTFFYRYQKALLEQGYVYIACPPLYKIEWGRNNVRYCYSDAEKDQIL
RGLPANAKYTIQRFKGLGEMMPEQLWETTMNPATRTLKQITIEDAVEADRIFTILMGDAVGPRREFIEAHSAHLDLAALD
I

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=621, Percent_Identity=24.9597423510467, Blast_Score=144, Evalue=3e-34,
Organism=Homo sapiens, GI19913408, Length=621, Percent_Identity=26.0869565217391, Blast_Score=141, Evalue=2e-33,
Organism=Escherichia coli, GI48994957, Length=561, Percent_Identity=53.475935828877, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1789408, Length=608, Percent_Identity=41.9407894736842, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17535065, Length=568, Percent_Identity=26.5845070422535, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI212645845, Length=536, Percent_Identity=25.1865671641791, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=181, Percent_Identity=31.4917127071823, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=580, Percent_Identity=26.3793103448276, Blast_Score=140, Evalue=9e-34,
Organism=Drosophila melanogaster, GI17136538, Length=565, Percent_Identity=27.0796460176991, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 70967; Mature: 70836

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENYGAEQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVYEVVDNSVDEALAGYCNDIQI
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEE
SLHPDGSVSVLDNGRGIPVDIHPKTGQSALVTVLTVLHAGGKFGGGGYKVSGGLHGVGVS
EECCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSEWVEVTVWREGKVYFQRFERGAVVGSLKSEPQPKGSNRRGTEVRFKPDGLIFPE
HHHHHHHHHEEEEEECCHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEECCCCCCCCC
REFDYAVLAARFRELAYLNAGLKITFSDLRRDPPHCETYQYAGGIKEYVAFINHEKTPIH
CCCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCC
SDIIYIQGERDGVEVEVALQWCSDVYTDTLIGFANNIRTNEGGTHLEGLKTSLTLTINSQ
CCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECHH
ARKLNKLKDSDSNLAGENVREGLTAIVSVKVPNPEFEGQTKTKLGNTEVRGIVQTLVSEA
HHHHHHHCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHH
LTEYLEFNPNVARGIVDKAIQSFNAAEAARRARELVRRKSALESSTLPGKLADCSCRDPA
HHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ESEVYLVEGDSAGGSAKQGRDRQFQAVLPLRGKILNIEKADPAKIYKNAEIQAMITALGL
CCCEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCHHHHHCCCHHHHHHHHHCC
GVKGDEFDPSQLRYHRIILMTDADVDGSHIRTLLLTFFYRYQKALLEQGYVYIACPPLYK
CCCCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
IEWGRNNVRYCYSDAEKDQILRGLPANAKYTIQRFKGLGEMMPEQLWETTMNPATRTLKQ
EEECCCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ITIEDAVEADRIFTILMGDAVGPRREFIEAHSAHLDLAALDI
HHHHHHHCHHHEEEEEECCCCCHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
TENYGAEQIQVLEGLEAVRKRPGMYIGSTGPKGLHHLVYEVVDNSVDEALAGYCNDIQI
CCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEE
SLHPDGSVSVLDNGRGIPVDIHPKTGQSALVTVLTVLHAGGKFGGGGYKVSGGLHGVGVS
EECCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSEWVEVTVWREGKVYFQRFERGAVVGSLKSEPQPKGSNRRGTEVRFKPDGLIFPE
HHHHHHHHHEEEEEECCHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEECCCCCCCCC
REFDYAVLAARFRELAYLNAGLKITFSDLRRDPPHCETYQYAGGIKEYVAFINHEKTPIH
CCCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCHHHCCHHHHHHHHCCCCCCCC
SDIIYIQGERDGVEVEVALQWCSDVYTDTLIGFANNIRTNEGGTHLEGLKTSLTLTINSQ
CCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECHH
ARKLNKLKDSDSNLAGENVREGLTAIVSVKVPNPEFEGQTKTKLGNTEVRGIVQTLVSEA
HHHHHHHCCCCCCCCCHHHHCCCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHH
LTEYLEFNPNVARGIVDKAIQSFNAAEAARRARELVRRKSALESSTLPGKLADCSCRDPA
HHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ESEVYLVEGDSAGGSAKQGRDRQFQAVLPLRGKILNIEKADPAKIYKNAEIQAMITALGL
CCCEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEECCCCHHHHHCCCHHHHHHHHHCC
GVKGDEFDPSQLRYHRIILMTDADVDGSHIRTLLLTFFYRYQKALLEQGYVYIACPPLYK
CCCCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
IEWGRNNVRYCYSDAEKDQILRGLPANAKYTIQRFKGLGEMMPEQLWETTMNPATRTLKQ
EEECCCCCEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ITIEDAVEADRIFTILMGDAVGPRREFIEAHSAHLDLAALDI
HHHHHHHCHHHEEEEEECCCCCHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]