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The map label for this gene is murD

Identifier: 86607791

GI number: 86607791

Start: 304468

End: 305910

Strand: Direct

Name: murD

Synonym: CYB_0292

Alternate gene names: 86607791

Gene position: 304468-305910 (Clockwise)

Preceding gene: 86607789

Following gene: 86607792

Centisome position: 9.99

GC content: 61.88

Gene sequence:

>1443_bases
GTGCAACATCAAGTTTTGGGGTTAGGCATTGCGGGGCTGGCGGCAGCGCGGCTGTTGCGGGCCCAGGGCTATGAAGTGCT
GGTTTGGGATGAGCAAGATTCCCCTCTTCTCAGACAGCGGCAGGCTGAGCTCAATCAAGAAGGGATCCCGGTTCGGCTGG
GGCAGCCTTTTCAACTGGCCGAAGGGGTGAAGCAGGTGGTGGTCAGTCCAGGCATTGCTTGGGATCACCCGCTGCTGCAG
GCGGTGCGTCAACAGGGAATCCCGGTGGTGGGGGAGGCGGAGCTGGCCTGGATCTATCTTGACCACCTACCCTGGGTGGG
GATCACCGGCACCAACGGCAAAAGCACCACCACAGCTCTGGTGGCGGAGATGTTCAAGGCGGCGGGGCTGCAAGGGATCC
CCTGCGGCAACATCGGCCTGCCCCTGTCGCAGGTGGCTTTGGCAACGCTCCAGGGAAAGCTCAAGCCGGACTGGATCGTG
GCGGAGCTGAGCAGCTACCAGTTGGAGGCCAGTAGCCGCCTCATGAGCAGCACTCCTGGAGGGCCGCCGCGCATTGGGGT
GTGGACCACCTTCACTCCCGATCACCTAGAACGGCATGGGACGCTGGAGCGCTATGCCAGCTTTAAGGCTCGCCTTTTGG
ATCGGGCCCAGTGGCGGGTGCTCAACGGCGAGGATCCCTACCTGTGCCGCCGCCGGCAGGATTGGGAAAAGACCTATTGG
ATCTCCCTTGCTGCCCCGCAACTCTGTTGCGATCAGGATCCCACCGCAACCCTTGACCTGCGGGAAAACCGCCTCTACAT
CCAGGGGGAGATGGTGGCGGAGCTGGAGGACTTTGCAGAACGCTGCCCTGGCCAACACAACCTGCAAAACCTGCTCTTGG
CGGCAGCAGCAGCACGTCTAGCTGGGCTCCCCAACGTTGCCATTCAAAAAGCCATCCGATCTTTTGCCGGCATGCCCCAT
CGGCTGGAGCGGGTGGCGCAAATCCAGGTGGGAGCCACCCCCATCCGCTTTGTCAACGACAGCAAGGCCACCAACTACGA
AGCCGGCTGGGTGGCACTCAACGCCCTCTCCCCACCCATTATTCTGATTGCCGGAGGAAGGGCTAAGCAGGGGGATCCCG
GCGCCTGGTTGCGGTTGATTCGGGCCAAGGTGGCCCGCGTGTTGCTGATGGGGGAGTCTGCCCCAGTGCTGGCAGAGGCC
CTCCAGGGGATCCACTATACTGACGTTGAGCTCGTGCCAACCCTAGATGTAGCGGTGGAAAGAGCCTTTGCAGCGGCTTG
TTCCCTTTCCCGTCAAGCTCAGAGGTTGGGGAAGCCCGCTCAGCCCATCACCGTTTTGCTGTCTCCCGCCTGTGCCAGTT
TTGACCAGTACTCCAGCTTTGAACACCGTGGCAACCATTTCCGAGCCTGCTGCCAAGCCCTTCAGGGATCCCTGGAATGC
TGA

Upstream 100 bases:

>100_bases
CGTAAGCAAGGGGAGTGTCGAACAAAAACATGCGGGTCAAGGACGTGACCGGTAAGATCCAAGCAGGGATCCCCAAATAA
AGAAAGAAGTAGAAACCAAC

Downstream 100 bases:

>100_bases
GGCTCGCTGGCTGCGCTGGCTCACCTTGATCTGGCTGGTGCTGGGGCTGATGATGCTGTTTTCCGCTTCCTACCCGGTGG
CTCAGCGGATCACTGGCGAT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MQHQVLGLGIAGLAAARLLRAQGYEVLVWDEQDSPLLRQRQAELNQEGIPVRLGQPFQLAEGVKQVVVSPGIAWDHPLLQ
AVRQQGIPVVGEAELAWIYLDHLPWVGITGTNGKSTTTALVAEMFKAAGLQGIPCGNIGLPLSQVALATLQGKLKPDWIV
AELSSYQLEASSRLMSSTPGGPPRIGVWTTFTPDHLERHGTLERYASFKARLLDRAQWRVLNGEDPYLCRRRQDWEKTYW
ISLAAPQLCCDQDPTATLDLRENRLYIQGEMVAELEDFAERCPGQHNLQNLLLAAAAARLAGLPNVAIQKAIRSFAGMPH
RLERVAQIQVGATPIRFVNDSKATNYEAGWVALNALSPPIILIAGGRAKQGDPGAWLRLIRAKVARVLLMGESAPVLAEA
LQGIHYTDVELVPTLDVAVERAFAAACSLSRQAQRLGKPAQPITVLLSPACASFDQYSSFEHRGNHFRACCQALQGSLEC

Sequences:

>Translated_480_residues
MQHQVLGLGIAGLAAARLLRAQGYEVLVWDEQDSPLLRQRQAELNQEGIPVRLGQPFQLAEGVKQVVVSPGIAWDHPLLQ
AVRQQGIPVVGEAELAWIYLDHLPWVGITGTNGKSTTTALVAEMFKAAGLQGIPCGNIGLPLSQVALATLQGKLKPDWIV
AELSSYQLEASSRLMSSTPGGPPRIGVWTTFTPDHLERHGTLERYASFKARLLDRAQWRVLNGEDPYLCRRRQDWEKTYW
ISLAAPQLCCDQDPTATLDLRENRLYIQGEMVAELEDFAERCPGQHNLQNLLLAAAAARLAGLPNVAIQKAIRSFAGMPH
RLERVAQIQVGATPIRFVNDSKATNYEAGWVALNALSPPIILIAGGRAKQGDPGAWLRLIRAKVARVLLMGESAPVLAEA
LQGIHYTDVELVPTLDVAVERAFAAACSLSRQAQRLGKPAQPITVLLSPACASFDQYSSFEHRGNHFRACCQALQGSLEC
>Mature_480_residues
MQHQVLGLGIAGLAAARLLRAQGYEVLVWDEQDSPLLRQRQAELNQEGIPVRLGQPFQLAEGVKQVVVSPGIAWDHPLLQ
AVRQQGIPVVGEAELAWIYLDHLPWVGITGTNGKSTTTALVAEMFKAAGLQGIPCGNIGLPLSQVALATLQGKLKPDWIV
AELSSYQLEASSRLMSSTPGGPPRIGVWTTFTPDHLERHGTLERYASFKARLLDRAQWRVLNGEDPYLCRRRQDWEKTYW
ISLAAPQLCCDQDPTATLDLRENRLYIQGEMVAELEDFAERCPGQHNLQNLLLAAAAARLAGLPNVAIQKAIRSFAGMPH
RLERVAQIQVGATPIRFVNDSKATNYEAGWVALNALSPPIILIAGGRAKQGDPGAWLRLIRAKVARVLLMGESAPVLAEA
LQGIHYTDVELVPTLDVAVERAFAAACSLSRQAQRLGKPAQPITVLLSPACASFDQYSSFEHRGNHFRACCQALQGSLEC

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=472, Percent_Identity=33.8983050847458, Blast_Score=174, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_SYNJB (Q2JHP5)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_476553.1
- ProteinModelPortal:   Q2JHP5
- SMR:   Q2JHP5
- STRING:   Q2JHP5
- GeneID:   3902139
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_0292
- TIGR:   CYB_0292
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   LPWVGIT
- PhylomeDB:   Q2JHP5
- ProtClustDB:   PRK02705
- BioCyc:   SSP321332:CYB_0292-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 52434; Mature: 52434

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHQVLGLGIAGLAAARLLRAQGYEVLVWDEQDSPLLRQRQAELNQEGIPVRLGQPFQLA
CCCEEEECCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCEECCCCHHHH
EGVKQVVVSPGIAWDHPLLQAVRQQGIPVVGEAELAWIYLDHLPWVGITGTNGKSTTTAL
HHHHHHHHCCCCCCCCHHHHHHHHCCCCEEECCCEEEEEECCCCEEEEECCCCCHHHHHH
VAEMFKAAGLQGIPCGNIGLPLSQVALATLQGKLKPDWIVAELSSYQLEASSRLMSSTPG
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCC
GPPRIGVWTTFTPDHLERHGTLERYASFKARLLDRAQWRVLNGEDPYLCRRRQDWEKTYW
CCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHCCCCCCCCEEE
ISLAAPQLCCDQDPTATLDLRENRLYIQGEMVAELEDFAERCPGQHNLQNLLLAAAAARL
EEECCCHHHCCCCCCEEEEEECCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AGLPNVAIQKAIRSFAGMPHRLERVAQIQVGATPIRFVNDSKATNYEAGWVALNALSPPI
CCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCEEEECCCCCCCCCCCEEEEECCCCCE
ILIAGGRAKQGDPGAWLRLIRAKVARVLLMGESAPVLAEALQGIHYTDVELVPTLDVAVE
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEECHHHHHHH
RAFAAACSLSRQAQRLGKPAQPITVLLSPACASFDQYSSFEHRGNHFRACCQALQGSLEC
HHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHCHHHHCCHHHCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQHQVLGLGIAGLAAARLLRAQGYEVLVWDEQDSPLLRQRQAELNQEGIPVRLGQPFQLA
CCCEEEECCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCEECCCCHHHH
EGVKQVVVSPGIAWDHPLLQAVRQQGIPVVGEAELAWIYLDHLPWVGITGTNGKSTTTAL
HHHHHHHHCCCCCCCCHHHHHHHHCCCCEEECCCEEEEEECCCCEEEEECCCCCHHHHHH
VAEMFKAAGLQGIPCGNIGLPLSQVALATLQGKLKPDWIVAELSSYQLEASSRLMSSTPG
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCC
GPPRIGVWTTFTPDHLERHGTLERYASFKARLLDRAQWRVLNGEDPYLCRRRQDWEKTYW
CCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHCCCCCCCCEEE
ISLAAPQLCCDQDPTATLDLRENRLYIQGEMVAELEDFAERCPGQHNLQNLLLAAAAARL
EEECCCHHHCCCCCCEEEEEECCEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
AGLPNVAIQKAIRSFAGMPHRLERVAQIQVGATPIRFVNDSKATNYEAGWVALNALSPPI
CCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCEEEECCCCCCCCCCCEEEEECCCCCE
ILIAGGRAKQGDPGAWLRLIRAKVARVLLMGESAPVLAEALQGIHYTDVELVPTLDVAVE
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEECHHHHHHH
RAFAAACSLSRQAQRLGKPAQPITVLLSPACASFDQYSSFEHRGNHFRACCQALQGSLEC
HHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHCHHHHCCHHHCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA