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The map label for this gene is trmL [H]

Identifier: 86607760

GI number: 86607760

Start: 276171

End: 276629

Strand: Reverse

Name: trmL [H]

Synonym: CYB_0261

Alternate gene names: 86607760

Gene position: 276629-276171 (Counterclockwise)

Preceding gene: 86607761

Following gene: 86607759

Centisome position: 9.08

GC content: 59.69

Gene sequence:

>459_bases
ATGCCCTTCCACGTTGTTTTGGTTGCCCCAGAGATCCCGGCCAATACGGGGAATATCGCCCGCACCTGTGCAGCCACCGA
TACGCCTCTCCATCTGGTGGATCCTCTGGGGTTTCGCCTTTCGGATCGCTACCTCAAGCGGGCGGGGCTGGACTACTGGC
AGCATGTCCAACTGTATCGCTACCCCTCTTGGTCTGCTCTGGTGCAGGCCCACCCTGAGGCCCGGTTCTGGTGTTTTAGT
GTGCGCGGCAAAGCCCTCTATACGGAAGTAACCTATCAGCCGGGAGACTGGTTGGTGTTCGGCAGCGAAAGCCGGGGGTT
GGATCCTCTCTTCTTGGAAACCTATCCCAGCGTGCGCATCCCACTGCGGGGGCCGGTGCGCAGCCTGAACCTCAGCAATG
CCGTAGCGATTGCCCTTTTTGAGGCGCTACGTCAGCAGGGATCCCTGACCAAAGAATAG

Upstream 100 bases:

>100_bases
GGCCTCTCCCTCTCGGCAACAGCCCAGCCGCAAGCCGGGCAAAGGTGGGGGTAAAAAAGGGGGCGGCAACCGCGGATTTG
GGGGCAAGTAGGATAGGACT

Downstream 100 bases:

>100_bases
GGGGTCGGGTTGACCCTGTTATGATAGGTGTTTCGCCCCGTCAGGAAGATGTGTGCCTTGCAGTATCTGCGCCCCGACTT
GCTCTACCTCAAGCCCTACG

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 152; Mature: 151

Protein sequence:

>152_residues
MPFHVVLVAPEIPANTGNIARTCAATDTPLHLVDPLGFRLSDRYLKRAGLDYWQHVQLYRYPSWSALVQAHPEARFWCFS
VRGKALYTEVTYQPGDWLVFGSESRGLDPLFLETYPSVRIPLRGPVRSLNLSNAVAIALFEALRQQGSLTKE

Sequences:

>Translated_152_residues
MPFHVVLVAPEIPANTGNIARTCAATDTPLHLVDPLGFRLSDRYLKRAGLDYWQHVQLYRYPSWSALVQAHPEARFWCFS
VRGKALYTEVTYQPGDWLVFGSESRGLDPLFLETYPSVRIPLRGPVRSLNLSNAVAIALFEALRQQGSLTKE
>Mature_151_residues
PFHVVLVAPEIPANTGNIARTCAATDTPLHLVDPLGFRLSDRYLKRAGLDYWQHVQLYRYPSWSALVQAHPEARFWCFSV
RGKALYTEVTYQPGDWLVFGSESRGLDPLFLETYPSVRIPLRGPVRSLNLSNAVAIALFEALRQQGSLTKE

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=148, Percent_Identity=42.5675675675676, Blast_Score=130, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17159; Mature: 17028

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFHVVLVAPEIPANTGNIARTCAATDTPLHLVDPLGFRLSDRYLKRAGLDYWQHVQLYR
CCEEEEEECCCCCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHCCCCHHHEEEEEE
YPSWSALVQAHPEARFWCFSVRGKALYTEVTYQPGDWLVFGSESRGLDPLFLETYPSVRI
CCCHHHEEEECCCCEEEEEEECCCEEEEEEEECCCCEEEECCCCCCCCEEEEECCCCEEE
PLRGPVRSLNLSNAVAIALFEALRQQGSLTKE
ECCCCCEECCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PFHVVLVAPEIPANTGNIARTCAATDTPLHLVDPLGFRLSDRYLKRAGLDYWQHVQLYR
CEEEEEECCCCCCCCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHCCCCHHHEEEEEE
YPSWSALVQAHPEARFWCFSVRGKALYTEVTYQPGDWLVFGSESRGLDPLFLETYPSVRI
CCCHHHEEEECCCCEEEEEEECCCEEEEEEEECCCCEEEECCCCCCCCEEEEECCCCEEE
PLRGPVRSLNLSNAVAIALFEALRQQGSLTKE
ECCCCCEECCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA