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The map label for this gene is walR [H]

Identifier: 86607748

GI number: 86607748

Start: 264506

End: 265258

Strand: Direct

Name: walR [H]

Synonym: CYB_0249

Alternate gene names: 86607748

Gene position: 264506-265258 (Clockwise)

Preceding gene: 86607747

Following gene: 86607749

Centisome position: 8.68

GC content: 60.56

Gene sequence:

>753_bases
ATGGCTCGCATACTCATTATTGAGGACGACCCTTCGATTCTGGATATCCTGCGCATCAATCTGGAGTTGGCGGGCTACGA
GGTTGTGAAGGCCACCGATGGGGTGCGCGGACAAGCACTGGCTCTGCAAGTTCTCCCCGATCTGATCATCCTCGACCTGA
TGCTGCCCCAGGTAGATGGCCTGACCGTTTGTCAGCGCTTGCGGCGGGAGGAGCGCACCAGCGAGATCCCGATTTTGATG
CTCACCGCCCTCGGCCAAACCCAGGACAAGATCGAGGGCTTTAATGCCGGTGCCGACGACTACCTGACCAAGCCTTTTGA
GGTGCAGGAGCTGCTGGTGCGGGTGCGGGCCCTGCTGCGTCGCTCAGACCGGATCCCACAGGCGGCCAAGCATGGGGAGA
TCCTCACCTATGGCCCTCTCACGCTGGTGCCGGAGCGCTTTGAGGCGTTGTGGTTCGGCAAAACGATCAAGCTCACCCAC
CTGGAGTTTGACCTGCTCCACTGTCTGATGCAGCGACATGGGCAGACCGTTTCCCCCAGCGAAATCCTGCAGGAGGTGTG
GGGCTACGAGCCCGACGACGACATCGAGACCATCCGTGTTCACATCCGCCACCTGCGCACCAAGCTGGAGCCGGATCCCC
GCCACCCCCAGTACATCAAAACCGTCTATGGGGCGGGCTACTGTCTGGAGTTGCCCAACATTGCCGATCCCGTCCCACCG
CAGCCAGAGCTGGCGAAAGATTCCCACAAATAA

Upstream 100 bases:

>100_bases
AATGAGGCTAAAATTAAGTAAACTTCCAGTCGTAAAGCTTCGGCTCTTTGTTAGCACATTTAACAAAACGTTTTATCCGC
AGTTTGGGCTGCAACCGGCT

Downstream 100 bases:

>100_bases
TGATTCTTTCATGATTCTTGAGGATGCTGCTCACGCAACTGCTAATCTAGGGGATGCCCTGCTGCAGATTGCGCTCTGAG
ATCTATGCCTGGTGCCATTC

Product: DNA-binding response regulator RpaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MARILIIEDDPSILDILRINLELAGYEVVKATDGVRGQALALQVLPDLIILDLMLPQVDGLTVCQRLRREERTSEIPILM
LTALGQTQDKIEGFNAGADDYLTKPFEVQELLVRVRALLRRSDRIPQAAKHGEILTYGPLTLVPERFEALWFGKTIKLTH
LEFDLLHCLMQRHGQTVSPSEILQEVWGYEPDDDIETIRVHIRHLRTKLEPDPRHPQYIKTVYGAGYCLELPNIADPVPP
QPELAKDSHK

Sequences:

>Translated_250_residues
MARILIIEDDPSILDILRINLELAGYEVVKATDGVRGQALALQVLPDLIILDLMLPQVDGLTVCQRLRREERTSEIPILM
LTALGQTQDKIEGFNAGADDYLTKPFEVQELLVRVRALLRRSDRIPQAAKHGEILTYGPLTLVPERFEALWFGKTIKLTH
LEFDLLHCLMQRHGQTVSPSEILQEVWGYEPDDDIETIRVHIRHLRTKLEPDPRHPQYIKTVYGAGYCLELPNIADPVPP
QPELAKDSHK
>Mature_249_residues
ARILIIEDDPSILDILRINLELAGYEVVKATDGVRGQALALQVLPDLIILDLMLPQVDGLTVCQRLRREERTSEIPILML
TALGQTQDKIEGFNAGADDYLTKPFEVQELLVRVRALLRRSDRIPQAAKHGEILTYGPLTLVPERFEALWFGKTIKLTHL
EFDLLHCLMQRHGQTVSPSEILQEVWGYEPDDDIETIRVHIRHLRTKLEPDPRHPQYIKTVYGAGYCLELPNIADPVPPQ
PELAKDSHK

Specific function: Member of the two-component regulatory system walK/walR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=36.8888888888889, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=37.6623376623377, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788394, Length=229, Percent_Identity=39.3013100436681, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=35.3711790393013, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786911, Length=233, Percent_Identity=36.9098712446352, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI87082012, Length=227, Percent_Identity=37.4449339207048, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=38.034188034188, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1787375, Length=233, Percent_Identity=33.4763948497854, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1789402, Length=227, Percent_Identity=35.6828193832599, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1790552, Length=231, Percent_Identity=36.7965367965368, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=31.858407079646, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1790863, Length=228, Percent_Identity=31.5789473684211, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=33.6244541484716, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI145693140, Length=239, Percent_Identity=28.0334728033473, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788550, Length=150, Percent_Identity=28.6666666666667, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788521, Length=225, Percent_Identity=30.2222222222222, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28443; Mature: 28312

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARILIIEDDPSILDILRINLELAGYEVVKATDGVRGQALALQVLPDLIILDLMLPQVDG
CEEEEEECCCCCEEHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
LTVCQRLRREERTSEIPILMLTALGQTQDKIEGFNAGADDYLTKPFEVQELLVRVRALLR
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHH
RSDRIPQAAKHGEILTYGPLTLVPERFEALWFGKTIKLTHLEFDLLHCLMQRHGQTVSPS
HHCCCCCHHHCCCEEEECCHHHCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCHH
EILQEVWGYEPDDDIETIRVHIRHLRTKLEPDPRHPQYIKTVYGAGYCLELPNIADPVPP
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCC
QPELAKDSHK
CCCCCCCCCC
>Mature Secondary Structure 
ARILIIEDDPSILDILRINLELAGYEVVKATDGVRGQALALQVLPDLIILDLMLPQVDG
EEEEEECCCCCEEHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
LTVCQRLRREERTSEIPILMLTALGQTQDKIEGFNAGADDYLTKPFEVQELLVRVRALLR
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHH
RSDRIPQAAKHGEILTYGPLTLVPERFEALWFGKTIKLTHLEFDLLHCLMQRHGQTVSPS
HHCCCCCHHHCCCEEEECCHHHCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCHH
EILQEVWGYEPDDDIETIRVHIRHLRTKLEPDPRHPQYIKTVYGAGYCLELPNIADPVPP
HHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCEEEECCCCCCCCCC
QPELAKDSHK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA