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The map label for this gene is pflA [H]

Identifier: 86607742

GI number: 86607742

Start: 255472

End: 256221

Strand: Reverse

Name: pflA [H]

Synonym: CYB_0243

Alternate gene names: 86607742

Gene position: 256221-255472 (Counterclockwise)

Preceding gene: 86607743

Following gene: 86607741

Centisome position: 8.41

GC content: 51.87

Gene sequence:

>750_bases
ATGGATACCGTTATGAACCCCCTGACACCAATTGCTGGGCGCATTCACTCGGTGGAAACCTGTGGCACCGTCGATGGGCC
AGGAATCCGCTTCGTAATTTTTACACAGGGTTGTCCACTTCGCTGTTTGTATTGCCACAACCCCGACTGTCGGGATCCGC
AGGCGGGACAGCTTGTTACGGTTGAGTCTTTGATTGCCGAGATCCAACGTTACAAGAATTACTTGCGAGGGGGGGGAGTT
ACGGCTACCGGGGGAGAGCCTTTAATGCAACCCACCTTTGTAGCTGAGATCTTCCGTCGCTGTCATGAGTTGGGCTTGCA
CACGGCTTTGGATACTTCTGGCTATGGGCAACTGGAAGCAGCCAAGCCTGTGTTGGAGCATACAGATTTGGTGTTGCTGG
ATATCAAGTCTTATCTGCCGGAGCTGTATCGAAAGGTTACCGGCGTTTCGCTAGAACCCACTCTTAATCTGGCCCGCTAT
TTAGACCAGATCCACAAGCCTACCTGGATTCGTTTTGTGCTGGTTCCCGGCTTGACGGATCCCGAGGAAAACATTAAGGG
TCTGGCCGAGTTTGTGGCCACCTTATCCAACGTGGAGCGAGTGGAAGTTTTGCCCTTTCACAAGATGGGGGAATACAAAT
GGCAGCAATTGGGATTGCCTTACACCCTTGCCGATGTCGATCCACCCACACCCGAACAGGTGAATCATGCATTACAAATC
TTTCGGGATCAAGGACTGGTGGCTATTTGA

Upstream 100 bases:

>100_bases
AAGAGAAATGGAAACTGTGTTCTATTTAACCTGAGTTAGCAATCTGGAAGAAAACCTCAAGATTGATTGATATTTTGCAA
AAGTCATCCAGGATAAAAGT

Downstream 100 bases:

>100_bases
CAATTCTGCAGTTGCGGCTTGGGTCAGCATAGCCAGGTCACGCTTAGATCCGAAGTGATTCACAGTTAATCTCTGCACCG
TCTCCAGATTGGCCCTGAGC

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MDTVMNPLTPIAGRIHSVETCGTVDGPGIRFVIFTQGCPLRCLYCHNPDCRDPQAGQLVTVESLIAEIQRYKNYLRGGGV
TATGGEPLMQPTFVAEIFRRCHELGLHTALDTSGYGQLEAAKPVLEHTDLVLLDIKSYLPELYRKVTGVSLEPTLNLARY
LDQIHKPTWIRFVLVPGLTDPEENIKGLAEFVATLSNVERVEVLPFHKMGEYKWQQLGLPYTLADVDPPTPEQVNHALQI
FRDQGLVAI

Sequences:

>Translated_249_residues
MDTVMNPLTPIAGRIHSVETCGTVDGPGIRFVIFTQGCPLRCLYCHNPDCRDPQAGQLVTVESLIAEIQRYKNYLRGGGV
TATGGEPLMQPTFVAEIFRRCHELGLHTALDTSGYGQLEAAKPVLEHTDLVLLDIKSYLPELYRKVTGVSLEPTLNLARY
LDQIHKPTWIRFVLVPGLTDPEENIKGLAEFVATLSNVERVEVLPFHKMGEYKWQQLGLPYTLADVDPPTPEQVNHALQI
FRDQGLVAI
>Mature_249_residues
MDTVMNPLTPIAGRIHSVETCGTVDGPGIRFVIFTQGCPLRCLYCHNPDCRDPQAGQLVTVESLIAEIQRYKNYLRGGGV
TATGGEPLMQPTFVAEIFRRCHELGLHTALDTSGYGQLEAAKPVLEHTDLVLLDIKSYLPELYRKVTGVSLEPTLNLARY
LDQIHKPTWIRFVLVPGLTDPEENIKGLAEFVATLSNVERVEVLPFHKMGEYKWQQLGLPYTLADVDPPTPEQVNHALQI
FRDQGLVAI

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=242, Percent_Identity=45.4545454545455, Blast_Score=229, Evalue=9e-62,
Organism=Escherichia coli, GI1790389, Length=266, Percent_Identity=27.8195488721804, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI226510931, Length=206, Percent_Identity=30.5825242718447, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27731; Mature: 27731

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTVMNPLTPIAGRIHSVETCGTVDGPGIRFVIFTQGCPLRCLYCHNPDCRDPQAGQLVT
CCCCCCCCHHHHHHHHCHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCEEE
VESLIAEIQRYKNYLRGGGVTATGGEPLMQPTFVAEIFRRCHELGLHTALDTSGYGQLEA
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCHH
AKPVLEHTDLVLLDIKSYLPELYRKVTGVSLEPTLNLARYLDQIHKPTWIRFVLVPGLTD
HHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCC
PEENIKGLAEFVATLSNVERVEVLPFHKMGEYKWQQLGLPYTLADVDPPTPEQVNHALQI
HHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCHHHHCCCCEEECCCCCCCHHHHHHHHHH
FRDQGLVAI
HHCCCCCCC
>Mature Secondary Structure
MDTVMNPLTPIAGRIHSVETCGTVDGPGIRFVIFTQGCPLRCLYCHNPDCRDPQAGQLVT
CCCCCCCCHHHHHHHHCHHHCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCEEE
VESLIAEIQRYKNYLRGGGVTATGGEPLMQPTFVAEIFRRCHELGLHTALDTSGYGQLEA
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCCHH
AKPVLEHTDLVLLDIKSYLPELYRKVTGVSLEPTLNLARYLDQIHKPTWIRFVLVPGLTD
HHHHHHHCCEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCC
PEENIKGLAEFVATLSNVERVEVLPFHKMGEYKWQQLGLPYTLADVDPPTPEQVNHALQI
HHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCHHHHCCCCEEECCCCCCCHHHHHHHHHH
FRDQGLVAI
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]