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The map label for this gene is sbp [H]

Identifier: 86607738

GI number: 86607738

Start: 248096

End: 249100

Strand: Reverse

Name: sbp [H]

Synonym: CYB_0239

Alternate gene names: 86607738

Gene position: 249100-248096 (Counterclockwise)

Preceding gene: 86607739

Following gene: 86607737

Centisome position: 8.18

GC content: 61.29

Gene sequence:

>1005_bases
ATGGGGCGGTGGCTGGGGATGGGATCCCTAATTTTTGGCCTTCTTCTTGGCCTCAGTGGTTGTGTCAGCGGTATGGGCTC
AGGGGGATCCAGAGAGGAAGTAGCGATTCTCCTGGCCTCCTTTGCGGTGACGCGGGATGCCTACCGGCAGATCATCCCTG
CTTTTGAGGCCGATTGGCTGGCGAAAACGGGGCAACGGGTGCGGGTGGAAAGCAGCTACGGCGGCTCGGCAACGCAATCG
CGGGCAGTTATTGACGGGCTGGATGCCGATGTGGTGGCCTTGGCTCTGGCCCTGGATGTGGAGCGCCTCGTGGCTGCCGG
TCTCATTCAGCCGGGATGGGAAGCCCGTACCCCCAACCAAGGGATCGTTACCCGTTCGGTGGCAGTAATTGTAACGCGGG
AGGGCAACCCCCAGGGGATCCGCTCTTGGCAAGATCTGGCTCGGCCCGGCTTGCAGGTGGTTACCGCCAACCCCAAGACC
TCAGGGGGCGCCCGCTGGAACTTTTTAGCCCTCTGGAGCGCTGTCCCTGAACCCCAGGCTCTGGCCTTCACCACCCAGGT
CTACAAGAATGTCCGCATTTTGCCGCGGGACTCACGAGAGGCCACCGATGTTTTTTTCCAAAAAGGGCAAGGGGATGCCC
TGCTCACCTACGAACATGAGGTGATCTTGGCCAGCCTGCGCGGCCAATCCCTGTTCTATGTGGTGCCGGATCCCAACATT
TCCATCGACAACCCCGTAGCCGTGGTGGATGCCAATGTGGATCGCAAAGGCACCCGTGCCGTGGCCGAAGCCTTTGTGGA
CTACCTATTCACCCCAGAAGCGCAGCGGGCCTTTGCCGAGGTGGGCTTTCGCCCGGTGGAGCCGACGGTGGCCCAAGAGT
TTGCCTGGCGGTACCCTCCTGTTGAGAAGCGGGTTACCGTCGCCGACCTGGGAGGCTGGGAGGTTGTCCAAAGCCGCTTT
TTTGCCGATGGGGCCTTGTTTGACCGCATCCAAGCTCAGTTGTAG

Upstream 100 bases:

>100_bases
CACTGAAGGGACACCTGCGAACAAATGGGGGATCCCCTCAGCAATCGAGGTGGTCTGGTCTGGGAGAGTTGGGGCCGGGA
TCCGCAGGCTGCCGGTGAGC

Downstream 100 bases:

>100_bases
GGAGTCTCCCTTGTTTTGGATCCCCGTTCAAGTTATTCTGGGAGCTGTATCAACGGTTTGTTGACAGGTTTACCGTAGAT
TAGCCGCGGGTTTAACTTCC

Product: sulfate ABC transporter, periplasmic sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MGRWLGMGSLIFGLLLGLSGCVSGMGSGGSREEVAILLASFAVTRDAYRQIIPAFEADWLAKTGQRVRVESSYGGSATQS
RAVIDGLDADVVALALALDVERLVAAGLIQPGWEARTPNQGIVTRSVAVIVTREGNPQGIRSWQDLARPGLQVVTANPKT
SGGARWNFLALWSAVPEPQALAFTTQVYKNVRILPRDSREATDVFFQKGQGDALLTYEHEVILASLRGQSLFYVVPDPNI
SIDNPVAVVDANVDRKGTRAVAEAFVDYLFTPEAQRAFAEVGFRPVEPTVAQEFAWRYPPVEKRVTVADLGGWEVVQSRF
FADGALFDRIQAQL

Sequences:

>Translated_334_residues
MGRWLGMGSLIFGLLLGLSGCVSGMGSGGSREEVAILLASFAVTRDAYRQIIPAFEADWLAKTGQRVRVESSYGGSATQS
RAVIDGLDADVVALALALDVERLVAAGLIQPGWEARTPNQGIVTRSVAVIVTREGNPQGIRSWQDLARPGLQVVTANPKT
SGGARWNFLALWSAVPEPQALAFTTQVYKNVRILPRDSREATDVFFQKGQGDALLTYEHEVILASLRGQSLFYVVPDPNI
SIDNPVAVVDANVDRKGTRAVAEAFVDYLFTPEAQRAFAEVGFRPVEPTVAQEFAWRYPPVEKRVTVADLGGWEVVQSRF
FADGALFDRIQAQL
>Mature_333_residues
GRWLGMGSLIFGLLLGLSGCVSGMGSGGSREEVAILLASFAVTRDAYRQIIPAFEADWLAKTGQRVRVESSYGGSATQSR
AVIDGLDADVVALALALDVERLVAAGLIQPGWEARTPNQGIVTRSVAVIVTREGNPQGIRSWQDLARPGLQVVTANPKTS
GGARWNFLALWSAVPEPQALAFTTQVYKNVRILPRDSREATDVFFQKGQGDALLTYEHEVILASLRGQSLFYVVPDPNIS
IDNPVAVVDANVDRKGTRAVAEAFVDYLFTPEAQRAFAEVGFRPVEPTVAQEFAWRYPPVEKRVTVADLGGWEVVQSRFF
ADGALFDRIQAQL

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=307, Percent_Identity=38.7622149837134, Blast_Score=228, Evalue=3e-61,
Organism=Escherichia coli, GI1788765, Length=314, Percent_Identity=32.8025477707006, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR006311
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36280; Mature: 36148

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRWLGMGSLIFGLLLGLSGCVSGMGSGGSREEVAILLASFAVTRDAYRQIIPAFEADWL
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AKTGQRVRVESSYGGSATQSRAVIDGLDADVVALALALDVERLVAAGLIQPGWEARTPNQ
HHCCCEEEEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GIVTRSVAVIVTREGNPQGIRSWQDLARPGLQVVTANPKTSGGARWNFLALWSAVPEPQA
CEEEEEEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCHH
LAFTTQVYKNVRILPRDSREATDVFFQKGQGDALLTYEHEVILASLRGQSLFYVVPDPNI
HHHHHHHHHCCEEEECCCCHHHHHHHCCCCCCEEEEEEEEEEEEEECCCEEEEEECCCCC
SIDNPVAVVDANVDRKGTRAVAEAFVDYLFTPEAQRAFAEVGFRPVEPTVAQEFAWRYPP
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCC
VEKRVTVADLGGWEVVQSRFFADGALFDRIQAQL
CCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure 
GRWLGMGSLIFGLLLGLSGCVSGMGSGGSREEVAILLASFAVTRDAYRQIIPAFEADWL
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
AKTGQRVRVESSYGGSATQSRAVIDGLDADVVALALALDVERLVAAGLIQPGWEARTPNQ
HHCCCEEEEECCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
GIVTRSVAVIVTREGNPQGIRSWQDLARPGLQVVTANPKTSGGARWNFLALWSAVPEPQA
CEEEEEEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCHH
LAFTTQVYKNVRILPRDSREATDVFFQKGQGDALLTYEHEVILASLRGQSLFYVVPDPNI
HHHHHHHHHCCEEEECCCCHHHHHHHCCCCCCEEEEEEEEEEEEEECCCEEEEEECCCCC
SIDNPVAVVDANVDRKGTRAVAEAFVDYLFTPEAQRAFAEVGFRPVEPTVAQEFAWRYPP
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHCCCCC
VEKRVTVADLGGWEVVQSRFFADGALFDRIQAQL
CCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]