Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 86607598

GI number: 86607598

Start: 112532

End: 114667

Strand: Direct

Name: slt [H]

Synonym: CYB_0096

Alternate gene names: 86607598

Gene position: 112532-114667 (Clockwise)

Preceding gene: 86607597

Following gene: 86607599

Centisome position: 3.69

GC content: 62.08

Gene sequence:

>2136_bases
ATGGGGATTCTGACAAGCAGCCAACGACGCTGGACAAGATGGGCTGCCGGTGTGCTGGCAGCAGGAGCGGCTCTGGGTGG
AGCTGTCTGGGGCTTCTACACCTCATCCTGGGCAGAAACCGTAGAAGTGGCCTCTGCACGAGAAGGCAGGGATCCGACGC
AACTGCCCCAAACTTCCACAACGGCCTTTCTGCGGGCCTACGCAGCGCTGCAAGCCGGCCAAGCGCAATCTGCCTTGAAG
GATTTGCAAGGGCTGGAAGAGAGCCTGCCGGTTTTGAGGGAAGAGATTTGGAAGCTCAGGGCCCAAGCCTATGAACAGTT
GCAGGATAAGGAAACAGCCCAAGGGATCTGGTGGCCCCAGATTTTGCAGGAATATCCCCACAGTCCTGTGGCCGCCTACG
CCCTCTGGGGGATGGGCCAGGTGGATCGGCTGCGGCAGCAATTTCCCACCCACCCGCTGACCGGACGTGCCCTCAAGCAT
TTGCTAGAGCTGAACCCCGACCGCTATGACCTGCTGCGGGATTTGGCCCAACATCACCCGCAAACCCCTGGGCTAACGCC
GTTGCTGGATCGCTGGCGGCAGGCTCAGGAGGGATCCCTCACGGCTTCCGACTGGCAGATCCTCGCCGATGCCTACTGGG
AGCAACGGGAGTACGGCAAAGCTGCCCGGGCCTATGGACGTGCCCCCGCCACCTCTCAGAACCTCTACCGCTGGGGCCGC
AGCCACCAGATCTCGCGGGAGTTTCCCCAGGCCAAAGCGGCTTACCAAGCGCTGCTGGCACAGTTTCCCGATGGGCCAGA
GGCTTCTCTCACCCGCCGCCGTTTGGCGGAACTGAGCGACCTGCCGACGGCGATCGACTTGCTTCGACAGGTGGGATCCG
GCTCAGATAAAGAAGCTCCAGAGGCGTTGCTTAGCCTCAGCCAGCTCTATGATCGCAACGCCAGCCCCCAATCGGCCCAG
GCTACTCGGCAAGCCCTTTGGGATCGCTTTCCGGCCAGCGAAGCTGCGGCCAACGCCGCCTGGACGGTGGCCTGGAGCCA
AGCACAAGCAGGCAATCTCAGCCAAGCCATCGCCCTGGCCCAACAGATCGGATCTGCTCAGCGGGACACGGAAATGGGGG
CACAACTGCTCTACTGGGCGGGCAAATGGCGGGAACAGCAGGGGGATGTCGCTGCAGCTCGGCAGACCTATCGGCAGGTA
CTGCGTCAGTTTCCCCACACCTACTACGGCTGGCGGTCGGCAGTGCAACTGGGCTGGCCGGTGGGGGATTTCTACAGCGG
GCGCAAAAGGGTTGAGGTGGATTTTCAGCCGGTGCGCCAACCGTTGCCGGGGGTCTCTGCAGTTACGCAAACCCTGCATT
TGATGGGAGCCAGCCAACTGGCCTGGGAACGCTGGCAGGGCGAAACTCCCGTGCAGCGGGATCCCGGCCAGATGAGCGTT
GCCGAGCGCTTTGTCACGGGCATTTTGCGCAACAGCGCCGGAGATCACCTGCGGGGCATTAACCAGGTGGCTGCTTTGCG
CTTCAGCGAAGAGCCGGATCCCCTGCTGGAGACGTTGAGGCAGCGCCAAGATTTCTGGCAGGCCATCTATCCGCTGCACT
ACTATGCGGGCCCCGACAATTCCTGGAGCGGGGATCCCTACGCCCAACCCGGCTTGGCCCACTGGTCACGGCAATTTAAC
CTCAATCCCTTGATGGTGGCGGCTTTGATCCGGCAGGAATCCCGCTTTGAGCCAGAGATCGTCTCGGCCTCGGGGGCTTT
GGGCTTGATGCAGGTGATGCCCGCTACTGGCCGCTGGATTGCCCAGCAGATCGGCCTGGCCCAGTACAGCCTCACCAACC
CCGCCGACAATCTCTACCTGGGATCCTGGTACTTCGACTACACCCACCGCACTTACCAAGACAACACCTTGCTGGCCCTG
GCCAGCTACAACGGTGGCCCCGGCAACGTGGCCAGATGGCTGAACCGCTTTGGCTTCGAGGATCCGGACGTGTTTGTGGA
GCAGATCCCCTTCCCCGAAACCCGGGGCTATGTGAAGTCCGTGTTTGGCAACTACTGGAACTACTGGCAGCTCTACACCC
AGGAGGGGCGCACTCTGGTCAGCCAGCGACTGCATAACCCCTCGTCTTCTTCATGA

Upstream 100 bases:

>100_bases
CCAGGTGAGCTTTCCAGCCGTTTCGCAAGTATCGAGTTGTCTGGTGACTCAACGCAGTCAGGTTGAGTATCGTAGGTCAG
AGGTGGTGGGGAGGTAAAGC

Downstream 100 bases:

>100_bases
CTTCCGGTTTTGTCGCGGGAGTGCTGCCGTAGAGGGAGCGGGCTTTTCCCCCGACAGAGCACAGTATTCTGTGAGAGTTA
CAGTGATTCCAAGGTTTACT

Product: Slt family transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MGILTSSQRRWTRWAAGVLAAGAALGGAVWGFYTSSWAETVEVASAREGRDPTQLPQTSTTAFLRAYAALQAGQAQSALK
DLQGLEESLPVLREEIWKLRAQAYEQLQDKETAQGIWWPQILQEYPHSPVAAYALWGMGQVDRLRQQFPTHPLTGRALKH
LLELNPDRYDLLRDLAQHHPQTPGLTPLLDRWRQAQEGSLTASDWQILADAYWEQREYGKAARAYGRAPATSQNLYRWGR
SHQISREFPQAKAAYQALLAQFPDGPEASLTRRRLAELSDLPTAIDLLRQVGSGSDKEAPEALLSLSQLYDRNASPQSAQ
ATRQALWDRFPASEAAANAAWTVAWSQAQAGNLSQAIALAQQIGSAQRDTEMGAQLLYWAGKWREQQGDVAAARQTYRQV
LRQFPHTYYGWRSAVQLGWPVGDFYSGRKRVEVDFQPVRQPLPGVSAVTQTLHLMGASQLAWERWQGETPVQRDPGQMSV
AERFVTGILRNSAGDHLRGINQVAALRFSEEPDPLLETLRQRQDFWQAIYPLHYYAGPDNSWSGDPYAQPGLAHWSRQFN
LNPLMVAALIRQESRFEPEIVSASGALGLMQVMPATGRWIAQQIGLAQYSLTNPADNLYLGSWYFDYTHRTYQDNTLLAL
ASYNGGPGNVARWLNRFGFEDPDVFVEQIPFPETRGYVKSVFGNYWNYWQLYTQEGRTLVSQRLHNPSSSS

Sequences:

>Translated_711_residues
MGILTSSQRRWTRWAAGVLAAGAALGGAVWGFYTSSWAETVEVASAREGRDPTQLPQTSTTAFLRAYAALQAGQAQSALK
DLQGLEESLPVLREEIWKLRAQAYEQLQDKETAQGIWWPQILQEYPHSPVAAYALWGMGQVDRLRQQFPTHPLTGRALKH
LLELNPDRYDLLRDLAQHHPQTPGLTPLLDRWRQAQEGSLTASDWQILADAYWEQREYGKAARAYGRAPATSQNLYRWGR
SHQISREFPQAKAAYQALLAQFPDGPEASLTRRRLAELSDLPTAIDLLRQVGSGSDKEAPEALLSLSQLYDRNASPQSAQ
ATRQALWDRFPASEAAANAAWTVAWSQAQAGNLSQAIALAQQIGSAQRDTEMGAQLLYWAGKWREQQGDVAAARQTYRQV
LRQFPHTYYGWRSAVQLGWPVGDFYSGRKRVEVDFQPVRQPLPGVSAVTQTLHLMGASQLAWERWQGETPVQRDPGQMSV
AERFVTGILRNSAGDHLRGINQVAALRFSEEPDPLLETLRQRQDFWQAIYPLHYYAGPDNSWSGDPYAQPGLAHWSRQFN
LNPLMVAALIRQESRFEPEIVSASGALGLMQVMPATGRWIAQQIGLAQYSLTNPADNLYLGSWYFDYTHRTYQDNTLLAL
ASYNGGPGNVARWLNRFGFEDPDVFVEQIPFPETRGYVKSVFGNYWNYWQLYTQEGRTLVSQRLHNPSSSS
>Mature_710_residues
GILTSSQRRWTRWAAGVLAAGAALGGAVWGFYTSSWAETVEVASAREGRDPTQLPQTSTTAFLRAYAALQAGQAQSALKD
LQGLEESLPVLREEIWKLRAQAYEQLQDKETAQGIWWPQILQEYPHSPVAAYALWGMGQVDRLRQQFPTHPLTGRALKHL
LELNPDRYDLLRDLAQHHPQTPGLTPLLDRWRQAQEGSLTASDWQILADAYWEQREYGKAARAYGRAPATSQNLYRWGRS
HQISREFPQAKAAYQALLAQFPDGPEASLTRRRLAELSDLPTAIDLLRQVGSGSDKEAPEALLSLSQLYDRNASPQSAQA
TRQALWDRFPASEAAANAAWTVAWSQAQAGNLSQAIALAQQIGSAQRDTEMGAQLLYWAGKWREQQGDVAAARQTYRQVL
RQFPHTYYGWRSAVQLGWPVGDFYSGRKRVEVDFQPVRQPLPGVSAVTQTLHLMGASQLAWERWQGETPVQRDPGQMSVA
ERFVTGILRNSAGDHLRGINQVAALRFSEEPDPLLETLRQRQDFWQAIYPLHYYAGPDNSWSGDPYAQPGLAHWSRQFNL
NPLMVAALIRQESRFEPEIVSASGALGLMQVMPATGRWIAQQIGLAQYSLTNPADNLYLGSWYFDYTHRTYQDNTLLALA
SYNGGPGNVARWLNRFGFEDPDVFVEQIPFPETRGYVKSVFGNYWNYWQLYTQEGRTLVSQRLHNPSSSS

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=139, Percent_Identity=40.2877697841727, Blast_Score=101, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80022; Mature: 79891

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGILTSSQRRWTRWAAGVLAAGAALGGAVWGFYTSSWAETVEVASAREGRDPTQLPQTST
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
TAFLRAYAALQAGQAQSALKDLQGLEESLPVLREEIWKLRAQAYEQLQDKETAQGIWWPQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ILQEYPHSPVAAYALWGMGQVDRLRQQFPTHPLTGRALKHLLELNPDRYDLLRDLAQHHP
HHHHCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCC
QTPGLTPLLDRWRQAQEGSLTASDWQILADAYWEQREYGKAARAYGRAPATSQNLYRWGR
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
SHQISREFPQAKAAYQALLAQFPDGPEASLTRRRLAELSDLPTAIDLLRQVGSGSDKEAP
CCCHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
EALLSLSQLYDRNASPQSAQATRQALWDRFPASEAAANAAWTVAWSQAQAGNLSQAIALA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCHHHHHHHH
QQIGSAQRDTEMGAQLLYWAGKWREQQGDVAAARQTYRQVLRQFPHTYYGWRSAVQLGWP
HHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
VGDFYSGRKRVEVDFQPVRQPLPGVSAVTQTLHLMGASQLAWERWQGETPVQRDPGQMSV
CHHHHCCCEEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
AERFVTGILRNSAGDHLRGINQVAALRFSEEPDPLLETLRQRQDFWQAIYPLHYYAGPDN
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCC
SWSGDPYAQPGLAHWSRQFNLNPLMVAALIRQESRFEPEIVSASGALGLMQVMPATGRWI
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHCCCCHHHH
AQQIGLAQYSLTNPADNLYLGSWYFDYTHRTYQDNTLLALASYNGGPGNVARWLNRFGFE
HHHHCHHHHCCCCCCCCCEEEEEEEECCCCEECCCEEEEEECCCCCCHHHHHHHHHHCCC
DPDVFVEQIPFPETRGYVKSVFGNYWNYWQLYTQEGRTLVSQRLHNPSSSS
CHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GILTSSQRRWTRWAAGVLAAGAALGGAVWGFYTSSWAETVEVASAREGRDPTQLPQTST
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
TAFLRAYAALQAGQAQSALKDLQGLEESLPVLREEIWKLRAQAYEQLQDKETAQGIWWPQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ILQEYPHSPVAAYALWGMGQVDRLRQQFPTHPLTGRALKHLLELNPDRYDLLRDLAQHHP
HHHHCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCC
QTPGLTPLLDRWRQAQEGSLTASDWQILADAYWEQREYGKAARAYGRAPATSQNLYRWGR
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCC
SHQISREFPQAKAAYQALLAQFPDGPEASLTRRRLAELSDLPTAIDLLRQVGSGSDKEAP
CCCHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHH
EALLSLSQLYDRNASPQSAQATRQALWDRFPASEAAANAAWTVAWSQAQAGNLSQAIALA
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCHHHHHHHH
QQIGSAQRDTEMGAQLLYWAGKWREQQGDVAAARQTYRQVLRQFPHTYYGWRSAVQLGWP
HHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
VGDFYSGRKRVEVDFQPVRQPLPGVSAVTQTLHLMGASQLAWERWQGETPVQRDPGQMSV
CHHHHCCCEEEEECHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
AERFVTGILRNSAGDHLRGINQVAALRFSEEPDPLLETLRQRQDFWQAIYPLHYYAGPDN
HHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCC
SWSGDPYAQPGLAHWSRQFNLNPLMVAALIRQESRFEPEIVSASGALGLMQVMPATGRWI
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHCCCCHHHH
AQQIGLAQYSLTNPADNLYLGSWYFDYTHRTYQDNTLLALASYNGGPGNVARWLNRFGFE
HHHHCHHHHCCCCCCCCCEEEEEEEECCCCEECCCEEEEEECCCCCCHHHHHHHHHHCCC
DPDVFVEQIPFPETRGYVKSVFGNYWNYWQLYTQEGRTLVSQRLHNPSSSS
CHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]