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The map label for this gene is rluE [H]

Identifier: 86607580

GI number: 86607580

Start: 90876

End: 91556

Strand: Direct

Name: rluE [H]

Synonym: CYB_0078

Alternate gene names: 86607580

Gene position: 90876-91556 (Clockwise)

Preceding gene: 86607578

Following gene: 86607583

Centisome position: 2.98

GC content: 58.15

Gene sequence:

>681_bases
TTGAAGCCGCTCTGCCAAATCTTTCTGCCAAATCTTAAAGATTGGAAAGGTTGGGTTCACAGTTTTGTTGTTTTGTGTGT
CATGCGCTATCTCAAGTTCTACAAGCCCTATGGCGTGCTCACCCAATTCACCGATCGAGAGGGACGGCCCACCCTCCAGT
CTTTTATCCCGGTGCCCAGGGTTTATCCGGTGGGGCGACTGGATCGCGACAGCGAAGGGCTGCTCCTGCTGACCGATGAT
GGTCGCCTCAATGCCCGCTTGATCGACCCCAAGTTCGGCCATGAGCGCATCTACTGGGTTCAGGTGGAAGGGATCCCCAC
CCCCCAAGCCCTGCAACAGCTGCAACAGGGGCTGATCATCCAAGGGCGAAAAACGCGCCCGGCCCAGGTCAAAGTCTTGG
CTGAGGAACCCACCCTGCCGCCACGGGATCCCCCCATCCGCTTTCGCCGCTCCATCCCCACCACCTGGCTGGAACTGACG
CTGCGGGAGGGTCGCAATCGCCAGGTGCGCCGCATGACTGCCGCTGTTGGGATCCCAACTTTGCGGTTGGTTCGGGTTGG
ACTGGGGCCCCTCTCTCTGGCGGGGCTTGCTCCTGGCCAGTGGCAAGATCTCAGTGAGGTAGAACTGGCCCAACTGCGGC
AATATTGTTTTTCTCAAACCATTAGAGTCATTTCAAGTTAG

Upstream 100 bases:

>100_bases
CCAAAACCCAGAGTAAGTTGTTGTCTATGGTCATGGGTTGAGCTCCGTCAAACGCTGCTGGCGATGGAGGGATATTGCTC
CTTAAGGCTTGATCTTACCG

Downstream 100 bases:

>100_bases
ATTAAGACGCTTCCTATTCGCTCGGCAGTGAGGAGCTGGGAAGCAAGACAACGGAACAGTTTTATTCCCTCTTAAATGAC
TATAAACTAGGACACTGCAT

Product: RNA pseudouridine synthase family protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKPLCQIFLPNLKDWKGWVHSFVVLCVMRYLKFYKPYGVLTQFTDREGRPTLQSFIPVPRVYPVGRLDRDSEGLLLLTDD
GRLNARLIDPKFGHERIYWVQVEGIPTPQALQQLQQGLIIQGRKTRPAQVKVLAEEPTLPPRDPPIRFRRSIPTTWLELT
LREGRNRQVRRMTAAVGIPTLRLVRVGLGPLSLAGLAPGQWQDLSEVELAQLRQYCFSQTIRVISS

Sequences:

>Translated_226_residues
MKPLCQIFLPNLKDWKGWVHSFVVLCVMRYLKFYKPYGVLTQFTDREGRPTLQSFIPVPRVYPVGRLDRDSEGLLLLTDD
GRLNARLIDPKFGHERIYWVQVEGIPTPQALQQLQQGLIIQGRKTRPAQVKVLAEEPTLPPRDPPIRFRRSIPTTWLELT
LREGRNRQVRRMTAAVGIPTLRLVRVGLGPLSLAGLAPGQWQDLSEVELAQLRQYCFSQTIRVISS
>Mature_226_residues
MKPLCQIFLPNLKDWKGWVHSFVVLCVMRYLKFYKPYGVLTQFTDREGRPTLQSFIPVPRVYPVGRLDRDSEGLLLLTDD
GRLNARLIDPKFGHERIYWVQVEGIPTPQALQQLQQGLIIQGRKTRPAQVKVLAEEPTLPPRDPPIRFRRSIPTTWLELT
LREGRNRQVRRMTAAVGIPTLRLVRVGLGPLSLAGLAPGQWQDLSEVELAQLRQYCFSQTIRVISS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=56.7415730337079, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1788510, Length=190, Percent_Identity=33.6842105263158, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787524, Length=193, Percent_Identity=37.3056994818653, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1790453, Length=183, Percent_Identity=32.7868852459016, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 25948; Mature: 25948

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLCQIFLPNLKDWKGWVHSFVVLCVMRYLKFYKPYGVLTQFTDREGRPTLQSFIPVPR
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHCCCCC
VYPVGRLDRDSEGLLLLTDDGRLNARLIDPKFGHERIYWVQVEGIPTPQALQQLQQGLII
CCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCCEE
QGRKTRPAQVKVLAEEPTLPPRDPPIRFRRSIPTTWLELTLREGRNRQVRRMTAAVGIPT
ECCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCH
LRLVRVGLGPLSLAGLAPGQWQDLSEVELAQLRQYCFSQTIRVISS
HHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKPLCQIFLPNLKDWKGWVHSFVVLCVMRYLKFYKPYGVLTQFTDREGRPTLQSFIPVPR
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCHHHHCCCCC
VYPVGRLDRDSEGLLLLTDDGRLNARLIDPKFGHERIYWVQVEGIPTPQALQQLQQGLII
CCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHHCCCEE
QGRKTRPAQVKVLAEEPTLPPRDPPIRFRRSIPTTWLELTLREGRNRQVRRMTAAVGIPT
ECCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCH
LRLVRVGLGPLSLAGLAPGQWQDLSEVELAQLRQYCFSQTIRVISS
HHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]