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The map label for this gene is gatA [H]

Identifier: 86606740

GI number: 86606740

Start: 2117544

End: 2118944

Strand: Reverse

Name: gatA [H]

Synonym: CYA_2100

Alternate gene names: 86606740

Gene position: 2118944-2117544 (Counterclockwise)

Preceding gene: 86606741

Following gene: 86606739

Centisome position: 72.25

GC content: 64.45

Gene sequence:

>1401_bases
ATGACCGGGGATCCCTACCTTCTCGGCAGCGCTGACGCGGTCTCTATTGCCAGGGCTGTCCGCCAGGGATCCGTGAGCGC
CCAAGCGGTGGTGGCCGCCTGTCTGGAGCGGATTCGCCGGCGGGATCCCCAGCTCAATGCCTTTACAGCTGTCTTGGAGG
AATCGGCCTTACAAGCGGCGGAGCAGGTGGATCGACAGGTGGCTCAAGGGATCCCGGTGGGCCCGCTGGCAGGGGTGCCC
TTTGCCGTCAAGAACTTGTTTGACGTGGCCGGCCTCACCACCCTAGCTGGCTCGGCGATCAACCGCGAGAATCCCCCGGC
CATCCAAGATGCAACGGTGGTGGCTCGTCTCAAGCAGGCAGGGGCGATCTTGGTGGGCACCCTCAATATGGACGAGTACG
CCTATGGCTTCGTCACCGAAAACAGCCACTACGGCCCCACCCACAACCCCCACGATCTCAACCGCAGTGCGGGGGGATCC
TCTGGCGGCTCGGCAGCAGCGGTGGCAGGAGGTTTGGTGCCCCTTGCCCTGGGCAGCGACACCAACGGCTCGATTCGGGT
ACCGGCCTCTTTGTGTGGCGTCTACGGCCTCAAGCCCACCTATGGCCGTCTGTCGCGGGCAGGGGTGGCTCTGTTTGCCC
CCAGCTTCGACCACGTGGGCCCCTTGGCCCGTTCGGTGGCCGATATTGCCCTCAGCTTCGATGTTATGCAAGGGCCGGAT
CCCCAAGATCCCGTCTGTACCCGGCGTCCTGTGGATCCCACCTTTGGGCAGTTGGGGCAGGGGATCGAAGGCTTGCGCAT
CGCGGTGGCGGATGATTACTTTGCCCGAGGGGCAGAGCTCCCCATCTTGGCCGGAGTAGAAAAAGTGGCCCAAGCCCTGG
GGGCCCGCCAGACTGTGCGGATCCCGGAAGCCCAGCGCGCCCGCGCCGCCGCCTACCTGATCACGGCTGCCGAGGGAGCC
AACCTGCACCTGCCCAACCTGCGAACTCGCCCCCACGACTTCGATCCGGCCACCCGGGATCGCTTTTTGGCCGGAGCTTT
GCTGCCCGCCAGTTGGATCTTGCAGGCGCAGCGCTTTCGCCGCTGGTACCGCCAGCAGGTGCAGACCATTTTTCAGGAGA
TCGATCTGTTCATTGCCCCCACCACCCCCTGCTCTGCCCCCTTACTTGGCCAAGAGAAGATGGTGATCGATGGGGTAGAA
GTTCTCACCCGGCCCAACCTCGGCCTCTACACTCAGCCCCTCTCGTTTATCGGCCTGCCGGTGCTTTCAGTGCCGTTGCG
CGAGAGCGGATCCCTGCCCTTTGGCGTACAACTGGTGGCCGCCCCCTATCAAGAAGCCAAACTGCTGCGGGCAGCCGCCT
ATTTGGAGAAGCTAGGGATCCCGGATGCGCGGCCTGCCTGA

Upstream 100 bases:

>100_bases
TGATCGAAAATTTTGCCCGCTTTCTGCCCATTGCTCAACAGGTGCTGGAGTTCCCGTTGCCGCCGATAGTTGAGCCAGAT
CCCACCTTTGACCCTCAGCC

Downstream 100 bases:

>100_bases
GCCAGGAGATGCTCCCTCTAGGCGCGTTTGAACCATATCTAGTGTTTTAATTTTGAATCCTTTGCTCCAGACGCTAAATA
TAATCTCCTCGATAGGGGTA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MTGDPYLLGSADAVSIARAVRQGSVSAQAVVAACLERIRRRDPQLNAFTAVLEESALQAAEQVDRQVAQGIPVGPLAGVP
FAVKNLFDVAGLTTLAGSAINRENPPAIQDATVVARLKQAGAILVGTLNMDEYAYGFVTENSHYGPTHNPHDLNRSAGGS
SGGSAAAVAGGLVPLALGSDTNGSIRVPASLCGVYGLKPTYGRLSRAGVALFAPSFDHVGPLARSVADIALSFDVMQGPD
PQDPVCTRRPVDPTFGQLGQGIEGLRIAVADDYFARGAELPILAGVEKVAQALGARQTVRIPEAQRARAAAYLITAAEGA
NLHLPNLRTRPHDFDPATRDRFLAGALLPASWILQAQRFRRWYRQQVQTIFQEIDLFIAPTTPCSAPLLGQEKMVIDGVE
VLTRPNLGLYTQPLSFIGLPVLSVPLRESGSLPFGVQLVAAPYQEAKLLRAAAYLEKLGIPDARPA

Sequences:

>Translated_466_residues
MTGDPYLLGSADAVSIARAVRQGSVSAQAVVAACLERIRRRDPQLNAFTAVLEESALQAAEQVDRQVAQGIPVGPLAGVP
FAVKNLFDVAGLTTLAGSAINRENPPAIQDATVVARLKQAGAILVGTLNMDEYAYGFVTENSHYGPTHNPHDLNRSAGGS
SGGSAAAVAGGLVPLALGSDTNGSIRVPASLCGVYGLKPTYGRLSRAGVALFAPSFDHVGPLARSVADIALSFDVMQGPD
PQDPVCTRRPVDPTFGQLGQGIEGLRIAVADDYFARGAELPILAGVEKVAQALGARQTVRIPEAQRARAAAYLITAAEGA
NLHLPNLRTRPHDFDPATRDRFLAGALLPASWILQAQRFRRWYRQQVQTIFQEIDLFIAPTTPCSAPLLGQEKMVIDGVE
VLTRPNLGLYTQPLSFIGLPVLSVPLRESGSLPFGVQLVAAPYQEAKLLRAAAYLEKLGIPDARPA
>Mature_465_residues
TGDPYLLGSADAVSIARAVRQGSVSAQAVVAACLERIRRRDPQLNAFTAVLEESALQAAEQVDRQVAQGIPVGPLAGVPF
AVKNLFDVAGLTTLAGSAINRENPPAIQDATVVARLKQAGAILVGTLNMDEYAYGFVTENSHYGPTHNPHDLNRSAGGSS
GGSAAAVAGGLVPLALGSDTNGSIRVPASLCGVYGLKPTYGRLSRAGVALFAPSFDHVGPLARSVADIALSFDVMQGPDP
QDPVCTRRPVDPTFGQLGQGIEGLRIAVADDYFARGAELPILAGVEKVAQALGARQTVRIPEAQRARAAAYLITAAEGAN
LHLPNLRTRPHDFDPATRDRFLAGALLPASWILQAQRFRRWYRQQVQTIFQEIDLFIAPTTPCSAPLLGQEKMVIDGVEV
LTRPNLGLYTQPLSFIGLPVLSVPLRESGSLPFGVQLVAAPYQEAKLLRAAAYLEKLGIPDARPA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=501, Percent_Identity=27.3453093812375, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI195972892, Length=247, Percent_Identity=36.0323886639676, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI166795287, Length=168, Percent_Identity=38.6904761904762, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=437, Percent_Identity=28.1464530892449, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17537465, Length=492, Percent_Identity=26.219512195122, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=196, Percent_Identity=35.2040816326531, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=248, Percent_Identity=28.6290322580645, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=193, Percent_Identity=35.2331606217617, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=193, Percent_Identity=35.2331606217617, Blast_Score=92, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71990152, Length=232, Percent_Identity=28.8793103448276, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=169, Percent_Identity=28.9940828402367, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=388, Percent_Identity=32.2164948453608, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323950, Length=461, Percent_Identity=27.765726681128, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=257, Percent_Identity=28.0155642023346, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648113, Length=464, Percent_Identity=33.1896551724138, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24648435, Length=489, Percent_Identity=28.0163599182004, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24648437, Length=489, Percent_Identity=28.0163599182004, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45550774, Length=489, Percent_Identity=28.0163599182004, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24644968, Length=498, Percent_Identity=27.3092369477912, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=29.2828685258964, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=29.2828685258964, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=29.2828685258964, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=29.2828685258964, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21356731, Length=254, Percent_Identity=33.4645669291339, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078093, Length=490, Percent_Identity=27.5510204081633, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24648441, Length=420, Percent_Identity=27.6190476190476, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648439, Length=420, Percent_Identity=27.6190476190476, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49268; Mature: 49137

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGDPYLLGSADAVSIARAVRQGSVSAQAVVAACLERIRRRDPQLNAFTAVLEESALQAA
CCCCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EQVDRQVAQGIPVGPLAGVPFAVKNLFDVAGLTTLAGSAINRENPPAIQDATVVARLKQA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
GAILVGTLNMDEYAYGFVTENSHYGPTHNPHDLNRSAGGSSGGSAAAVAGGLVPLALGSD
CEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEECCC
TNGSIRVPASLCGVYGLKPTYGRLSRAGVALFAPSFDHVGPLARSVADIALSFDVMQGPD
CCCCEECCHHHHHHCCCCCCHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
PQDPVCTRRPVDPTFGQLGQGIEGLRIAVADDYFARGAELPILAGVEKVAQALGARQTVR
CCCCCCCCCCCCCCHHHHCCCCCCEEEEEECHHHHCCCCCCHHHHHHHHHHHHCCCCEEE
IPEAQRARAAAYLITAAEGANLHLPNLRTRPHDFDPATRDRFLAGALLPASWILQAQRFR
CCCHHHHHHEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RWYRQQVQTIFQEIDLFIAPTTPCSAPLLGQEKMVIDGVEVLTRPNLGLYTQPLSFIGLP
HHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEECCHHHHCCCCCCCEECCHHHHCCC
VLSVPLRESGSLPFGVQLVAAPYQEAKLLRAAAYLEKLGIPDARPA
HHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TGDPYLLGSADAVSIARAVRQGSVSAQAVVAACLERIRRRDPQLNAFTAVLEESALQAA
CCCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
EQVDRQVAQGIPVGPLAGVPFAVKNLFDVAGLTTLAGSAINRENPPAIQDATVVARLKQA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHC
GAILVGTLNMDEYAYGFVTENSHYGPTHNPHDLNRSAGGSSGGSAAAVAGGLVPLALGSD
CEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEECCC
TNGSIRVPASLCGVYGLKPTYGRLSRAGVALFAPSFDHVGPLARSVADIALSFDVMQGPD
CCCCEECCHHHHHHCCCCCCHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCC
PQDPVCTRRPVDPTFGQLGQGIEGLRIAVADDYFARGAELPILAGVEKVAQALGARQTVR
CCCCCCCCCCCCCCHHHHCCCCCCEEEEEECHHHHCCCCCCHHHHHHHHHHHHCCCCEEE
IPEAQRARAAAYLITAAEGANLHLPNLRTRPHDFDPATRDRFLAGALLPASWILQAQRFR
CCCHHHHHHEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RWYRQQVQTIFQEIDLFIAPTTPCSAPLLGQEKMVIDGVEVLTRPNLGLYTQPLSFIGLP
HHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCEECCHHHHCCCCCCCEECCHHHHCCC
VLSVPLRESGSLPFGVQLVAAPYQEAKLLRAAAYLEKLGIPDARPA
HHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA