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The map label for this gene is btuF [H]

Identifier: 86606506

GI number: 86606506

Start: 1860270

End: 1861091

Strand: Reverse

Name: btuF [H]

Synonym: CYA_1855

Alternate gene names: 86606506

Gene position: 1861091-1860270 (Counterclockwise)

Preceding gene: 86606507

Following gene: 86606504

Centisome position: 63.46

GC content: 61.07

Gene sequence:

>822_bases
ATGATTGGCAGACATGTTCCGCGACCGCGATTGCCAGAGCGCATCGTGTGCTTGACGGAGGAGACGGCAGAGACGCTCTA
CGCCTTGGGGGCTGGGGATCTCGTCGTCGGCATTTCTGGATTTACGGTGCGTCCGCCGGAGGCGCGGAAGAAGCCGAAGG
TGTCCACCCTTCTGGATGCCAACATCGAACGGATTTTGGCGCTGCAGCCCGATGTTGTGCTTGCATGGTCGGATCTGCAG
GCAAACATTTGCGCTGAGCTCATTCGCCAGGGGGTGGAGGTTGTCTGCTTCAACCAGCGCACGGTTGAGGGGATCCTCTC
CATGATCCTGCGCCTTGGGGCACTGGTAGGGCGAGCTGCTCAGGCACGGGCATATGTGCGCTCCTTGCGGGAGGGATTGC
ACCGAGCTATGCAGCGTGGACAGCAACGGCGCCGCCGTCCACGGGTCTACTTCGAGGAGTGGGACGACCCCTTGATTACA
GGCATCGGCTGGATCAGCGAACTCATTCGTTTGTGTGGAGCTGAAGACATCTTTTCCGAATTCAGTGCCTATCCCGATGC
ACGGCGCCGGATTATAGCTGAACCGGCAGAGGTCGTGCGGCGCGCTCCAGACATCATCCTGGTGTCCTGGTGTGGCAAGA
GGTTCAAGCCTGAGCGGCTTCACAGCCGCCCTGGGTGGGAGACTATCCCGGCGGTCTGGAGCGGACACGTCTACGAGCTG
CCGTCAGAGGTGATTCTGCAGCCCGGTCCGGCTGCCTTGACCGATGGGGTGCAGTTGCTGCTGCAGATCTTTGACCGCTG
GGAGGCCCAATCCTCCGCCTAA

Upstream 100 bases:

>100_bases
GCGAGAGCTCTTGCATGAGCGTCGGGGTGAGGCATCGAAGCGGGCAGCCTAGAGCGCCCATTTGGCAAACCAGTTCGTAG
ACTCGCTCGAAGAAATCCCG

Downstream 100 bases:

>100_bases
GCTTTGATGCTGCTTTCGATAGGGTTGTGGGCCTGGACGGCGTCATACTCGAGCCCGAAGTCTTCTAGCGCGTACAGCAC
TGCCCGATTGTGGCTGCTGC

Product: metal chelate ABC transporter, metal chelate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MIGRHVPRPRLPERIVCLTEETAETLYALGAGDLVVGISGFTVRPPEARKKPKVSTLLDANIERILALQPDVVLAWSDLQ
ANICAELIRQGVEVVCFNQRTVEGILSMILRLGALVGRAAQARAYVRSLREGLHRAMQRGQQRRRRPRVYFEEWDDPLIT
GIGWISELIRLCGAEDIFSEFSAYPDARRRIIAEPAEVVRRAPDIILVSWCGKRFKPERLHSRPGWETIPAVWSGHVYEL
PSEVILQPGPAALTDGVQLLLQIFDRWEAQSSA

Sequences:

>Translated_273_residues
MIGRHVPRPRLPERIVCLTEETAETLYALGAGDLVVGISGFTVRPPEARKKPKVSTLLDANIERILALQPDVVLAWSDLQ
ANICAELIRQGVEVVCFNQRTVEGILSMILRLGALVGRAAQARAYVRSLREGLHRAMQRGQQRRRRPRVYFEEWDDPLIT
GIGWISELIRLCGAEDIFSEFSAYPDARRRIIAEPAEVVRRAPDIILVSWCGKRFKPERLHSRPGWETIPAVWSGHVYEL
PSEVILQPGPAALTDGVQLLLQIFDRWEAQSSA
>Mature_273_residues
MIGRHVPRPRLPERIVCLTEETAETLYALGAGDLVVGISGFTVRPPEARKKPKVSTLLDANIERILALQPDVVLAWSDLQ
ANICAELIRQGVEVVCFNQRTVEGILSMILRLGALVGRAAQARAYVRSLREGLHRAMQRGQQRRRRPRVYFEEWDDPLIT
GIGWISELIRLCGAEDIFSEFSAYPDARRRIIAEPAEVVRRAPDIILVSWCGKRFKPERLHSRPGWETIPAVWSGHVYEL
PSEVILQPGPAALTDGVQLLLQIFDRWEAQSSA

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=202, Percent_Identity=28.7128712871287, Blast_Score=71, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 30743; Mature: 30743

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRHVPRPRLPERIVCLTEETAETLYALGAGDLVVGISGFTVRPPEARKKPKVSTLLDA
CCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCCHHHHHHH
NIERILALQPDVVLAWSDLQANICAELIRQGVEVVCFNQRTVEGILSMILRLGALVGRAA
HHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
QARAYVRSLREGLHRAMQRGQQRRRRPRVYFEEWDDPLITGIGWISELIRLCGAEDIFSE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHCCHHHHHH
FSAYPDARRRIIAEPAEVVRRAPDIILVSWCGKRFKPERLHSRPGWETIPAVWSGHVYEL
HHCCCHHHHHHHCCHHHHHHCCCCEEEEECCCCCCCHHHHCCCCCCCHHHHHHCCCHHCC
PSEVILQPGPAALTDGVQLLLQIFDRWEAQSSA
CHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIGRHVPRPRLPERIVCLTEETAETLYALGAGDLVVGISGFTVRPPEARKKPKVSTLLDA
CCCCCCCCCCCCCCEEEECHHHHHHHHHCCCCCEEEECCCCEECCCCCCCCCCHHHHHHH
NIERILALQPDVVLAWSDLQANICAELIRQGVEVVCFNQRTVEGILSMILRLGALVGRAA
HHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHHHHHHHH
QARAYVRSLREGLHRAMQRGQQRRRRPRVYFEEWDDPLITGIGWISELIRLCGAEDIFSE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHCCHHHHHH
FSAYPDARRRIIAEPAEVVRRAPDIILVSWCGKRFKPERLHSRPGWETIPAVWSGHVYEL
HHCCCHHHHHHHCCHHHHHHCCCCEEEEECCCCCCCHHHHCCCCCCCHHHHHHCCCHHCC
PSEVILQPGPAALTDGVQLLLQIFDRWEAQSSA
CHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]