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The map label for this gene is pyrF

Identifier: 86606367

GI number: 86606367

Start: 1720790

End: 1721488

Strand: Reverse

Name: pyrF

Synonym: CYA_1712

Alternate gene names: 86606367

Gene position: 1721488-1720790 (Counterclockwise)

Preceding gene: 86606368

Following gene: 86606366

Centisome position: 58.7

GC content: 64.09

Gene sequence:

>699_bases
ATGCAGGGTAGCCGCCTCATTGTTGCTCTAGATACCGCTTCTTGGGAGCAGGCCCTGAAGTGGGTGGATCGCCTGCCCCA
GGTGCTGTGGTGGAAGGTGGGCCTGGAGCTGTTTACGGCTGTTGGCCCTCAGATTTTGCAGGCCCTTAAGGAACGGGGCA
AGCGCATTTTTCTCGATTTAAAGCTGCACGACATTCCCCACACAGTGGGGCGGGCCGCTCAGGTGGCCGTTGGTTATGGG
GTGGATCTGCTCACCGTTCATGCCGCTGGCGGCAGTGCCATGTTGCGGGCAGCAGTGGCCGCCACCCAGGGCAGTTCTTG
CCGGCTGTTGGCAGTTACTCTTCTCACCAGCCTTGGCCCCGACCAGGTGCGGCAGGAGTTGGGCGTTGAACGAGATCCCG
GCGACTATGTGTTGCAGTTGGCGCGGCTAGCTCTGGATCAAGGCGTGAGCGGGCTGGTCTGCTCCCCCCAGGAAGTGGGA
CGGCTACGGCAGGCTTTGGGGCCGGAGGTGCTGCTGGTAACCCCAGGGATCCGCCTTGGGGATCCAGAAGCGGAGGACGA
CCAGCGGCGGGTCTGCACCCCCGCGGCAGCGCTGCGGGCGGGGGCCGATTATTTGGTGGTGGGCCGCCCCATCACGGCGG
CTGCGGATCCCGTTTCGGCCTGGCAACGCTTTCAAGCTGTAGAGGAAGCGATCGGCTGA

Upstream 100 bases:

>100_bases
TGCGCTGTGCCGTCAACCCCTATGGCCCCTGTGAAGGCTGCTCCCACTATGAGCCGCGAGAGCGGAGCCCTTCGCGTTAG
CCGGCCTGAGGGCTTTCGCC

Downstream 100 bases:

>100_bases
GGATTGGGGCAATGCCGTTCCTAGGCTGGTGTAGCCGGCGAAGAAGACAAGCGGGCGGGTATCTTTTCCCCCACCCCGCC
CGAGGGGGGTGGCGGATAGG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MQGSRLIVALDTASWEQALKWVDRLPQVLWWKVGLELFTAVGPQILQALKERGKRIFLDLKLHDIPHTVGRAAQVAVGYG
VDLLTVHAAGGSAMLRAAVAATQGSSCRLLAVTLLTSLGPDQVRQELGVERDPGDYVLQLARLALDQGVSGLVCSPQEVG
RLRQALGPEVLLVTPGIRLGDPEAEDDQRRVCTPAAALRAGADYLVVGRPITAAADPVSAWQRFQAVEEAIG

Sequences:

>Translated_232_residues
MQGSRLIVALDTASWEQALKWVDRLPQVLWWKVGLELFTAVGPQILQALKERGKRIFLDLKLHDIPHTVGRAAQVAVGYG
VDLLTVHAAGGSAMLRAAVAATQGSSCRLLAVTLLTSLGPDQVRQELGVERDPGDYVLQLARLALDQGVSGLVCSPQEVG
RLRQALGPEVLLVTPGIRLGDPEAEDDQRRVCTPAAALRAGADYLVVGRPITAAADPVSAWQRFQAVEEAIG
>Mature_232_residues
MQGSRLIVALDTASWEQALKWVDRLPQVLWWKVGLELFTAVGPQILQALKERGKRIFLDLKLHDIPHTVGRAAQVAVGYG
VDLLTVHAAGGSAMLRAAVAATQGSSCRLLAVTLLTSLGPDQVRQELGVERDPGDYVLQLARLALDQGVSGLVCSPQEVG
RLRQALGPEVLLVTPGIRLGDPEAEDDQRRVCTPAAALRAGADYLVVGRPITAAADPVSAWQRFQAVEEAIG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=228, Percent_Identity=43.859649122807, Blast_Score=168, Evalue=2e-43,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_SYNJA (Q2JTW6)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_475130.1
- ProteinModelPortal:   Q2JTW6
- SMR:   Q2JTW6
- STRING:   Q2JTW6
- GeneID:   3897479
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_1712
- TIGR:   CYA_1712
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK750486
- BioCyc:   SSP321327:CYA_1712-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24821; Mature: 24821

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00156 OMPDECASE; PS00184 GARS

Important sites: ACT_SITE 61-61 BINDING 11-11 BINDING 32-32 BINDING 116-116 BINDING 178-178 BINDING 188-188 BINDING 208-208 BINDING 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGSRLIVALDTASWEQALKWVDRLPQVLWWKVGLELFTAVGPQILQALKERGKRIFLDL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEE
KLHDIPHTVGRAAQVAVGYGVDLLTVHAAGGSAMLRAAVAATQGSSCRLLAVTLLTSLGP
EECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCH
DQVRQELGVERDPGDYVLQLARLALDQGVSGLVCSPQEVGRLRQALGPEVLLVTPGIRLG
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHCCCCEEEECCCCCCC
DPEAEDDQRRVCTPAAALRAGADYLVVGRPITAAADPVSAWQRFQAVEEAIG
CCCCCCCHHHHCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQGSRLIVALDTASWEQALKWVDRLPQVLWWKVGLELFTAVGPQILQALKERGKRIFLDL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEE
KLHDIPHTVGRAAQVAVGYGVDLLTVHAAGGSAMLRAAVAATQGSSCRLLAVTLLTSLGP
EECCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCH
DQVRQELGVERDPGDYVLQLARLALDQGVSGLVCSPQEVGRLRQALGPEVLLVTPGIRLG
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHHCCCCEEEECCCCCCC
DPEAEDDQRRVCTPAAALRAGADYLVVGRPITAAADPVSAWQRFQAVEEAIG
CCCCCCCHHHHCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA