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The map label for this gene is gyrB

Identifier: 86606189

GI number: 86606189

Start: 1535547

End: 1537475

Strand: Reverse

Name: gyrB

Synonym: CYA_1523

Alternate gene names: 86606189

Gene position: 1537475-1535547 (Counterclockwise)

Preceding gene: 86606190

Following gene: 86606186

Centisome position: 52.42

GC content: 61.22

Gene sequence:

>1929_bases
ATGACGGAGAACTACGGTGCAGAGCAGATCCAAGTTTTGGAAGGCTTGGAGGCTGTGCGCAGGCGGCCTGGCATGTACAT
TGGCTCCACTGGGCCGAAGGGATTGCACCACCTGGTGTACGAGGTGGTGGACAACTCCGTCGATGAAGCTCTGGCTGGCT
ACTGCAACGAAATTGAGGTTACTCTCCACCCCGATGGCTCGGTATCGGTGCTGGACAATGGCCGCGGCATCCCGGTAGAC
ATCCACCCCAAGACCGGGCAGTCGGCGCTGGTGACGGTCATGACTGTGTTGCACGCTGGCGGCAAATTCGGCGGCGGCGG
CTACAAGGTCTCCGGGGGGCTGCACGGCGTTGGGGTATCGGTGGTGAATGCCCTCTCCGAGTGGTTGGAGGCCAGAGTCT
GGCGGGAGGGCAAGGTTTACTTCCAGCGCTTCGAGCGAGGGGCGGTGGTGGGATCCCTTCAGTCCGAGCCGCAGCCTGCC
GGTGCCCAGCGGCGGGGCACGGAGGTGCGCTTTAAGCCGGATGAGCTCATCTTCCCGGAGCGGGAGTTTGACTACAGCCT
GTTGGCGGCCCGCTTGCGCGAGCTGGCCTACCTCAATGCCGGCTTGAAAATCATCTTCAGCGATCTGCGGCGGGATCCGC
CTCACCGCGAAGTCTATCAATTTGCCGGCGGCATTAAGGAATATGTGGCCTTCATCAACCACGAAAAAACCCCCATCCAC
CCCGATATTATCTATGTGCGCGGGGAGCGGGATGGCGTCGAGGTAGAAGCGGCATTGCAGTGGTGCGCGGATGTCTACAC
CGATACCTTGATTGGCTTTGCCAACAACATCCGCACCACTGAGGGGGGCACCCACTTGGAGGGATTGAAGACCGCCCTTA
CCCTCACCATCAACAGCCAAGCCCGCAAGCTCAACAAACTCAAGGACTCCGACAGCAACCTGGCGGGAGAAAATGTGCGG
GAGGGGCTGACCGCCATCCTTGCAGTCAAGGTGCCCAACCCTGAGTTTGAGGGGCAGACCAAAACCAAGCTGGGCAACAC
CGAGGTGCGGGGCATTGTCCAGACCCTTGTCTCGGAAGCCCTTACAGAATACCTAGAGTTCAACCCCAACGTGGCCAGGG
GGATCGTCGAGAAGGCCATCCAGTCCTTCAACGCGGCCGAGGCGGCCCGTCGGGCCCGGGAGCTGGTGCGCCGTAAGTCT
GCCCTGGAATCTTCCACCCTGCCGGGGAAACTGGCCGACTGCAGTTGCCGGGATCCGGCGGAGTCGGAGGTGTACTTGGT
GGAGGGCGACTCGGCAGGGGGCAGCGCCAAGCAGGGACGGGATCGCCAGTTTCAGGCGGTGCTGCCTTTGCGGGGCAAGA
TCCTCAACATCGAGAAGGCGGATCCCGCCAAGATCTACAAAAACGCCGAGATTCAGGCCATGATCACAGCGCTGGGCCTG
GGGGTCAAGGGCGAGGAGTTCGATCCCAGCCAACTGCGCTACCACCGCATCATCCTCATGACCGACGCCGACGTGGACGG
CAGCCACATCCGCACCCTGCTGCTCACCTTCTTTTACCGCTATCAGAAGGCGCTTTTGGAGCAGGGCTACGTCTACATTG
CCTGTCCGCCGCTTTACAAGATCGAGTGGGGCCGCAACAACGTGCGCTACTGCTACAACGAGGCCGAGAAAGAGCAAGTC
CTGCGCAGCCTGCCGGCCAACACCAAGTACACCATCCAGCGCTTCAAGGGCTTGGGGGAAATGATGCCGGAGCAGCTTTG
GGAAACCACCATGAACCCGGCAACGCGCGTCCTCAAGCAGATCACCATCGAAGACGCAGCCGAAGCCGATCGCATTTTCA
CCATCCTGATGGGGGATGCCGTCGGGCCGCGTCGGGAGTTCATCGAGGCCCACAGTGCCCAGTTGGATCTGGCGGCTCTG
GATATTTGA

Upstream 100 bases:

>100_bases
GCAGGTTTTTGGCATTTGGATTATCTCTGGCTTCTGGCGTCAATTTCTCTGGGTTTAGGTCGGCGGGGGCCGGGATCTCT
CTTCGGGATTGGAGTTACGA

Downstream 100 bases:

>100_bases
CGGGGGAGATGTGGTAGCTGCCATCGGCCTGCACCCAGGCAATCTCCGGGATCCGGCAGCGGCGGGCGTAGGCCTCGATT
TCTTCGGGGGAGCGGTCCAG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MTENYGAEQIQVLEGLEAVRRRPGMYIGSTGPKGLHHLVYEVVDNSVDEALAGYCNEIEVTLHPDGSVSVLDNGRGIPVD
IHPKTGQSALVTVMTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSEWLEARVWREGKVYFQRFERGAVVGSLQSEPQPA
GAQRRGTEVRFKPDELIFPEREFDYSLLAARLRELAYLNAGLKIIFSDLRRDPPHREVYQFAGGIKEYVAFINHEKTPIH
PDIIYVRGERDGVEVEAALQWCADVYTDTLIGFANNIRTTEGGTHLEGLKTALTLTINSQARKLNKLKDSDSNLAGENVR
EGLTAILAVKVPNPEFEGQTKTKLGNTEVRGIVQTLVSEALTEYLEFNPNVARGIVEKAIQSFNAAEAARRARELVRRKS
ALESSTLPGKLADCSCRDPAESEVYLVEGDSAGGSAKQGRDRQFQAVLPLRGKILNIEKADPAKIYKNAEIQAMITALGL
GVKGEEFDPSQLRYHRIILMTDADVDGSHIRTLLLTFFYRYQKALLEQGYVYIACPPLYKIEWGRNNVRYCYNEAEKEQV
LRSLPANTKYTIQRFKGLGEMMPEQLWETTMNPATRVLKQITIEDAAEADRIFTILMGDAVGPRREFIEAHSAQLDLAAL
DI

Sequences:

>Translated_642_residues
MTENYGAEQIQVLEGLEAVRRRPGMYIGSTGPKGLHHLVYEVVDNSVDEALAGYCNEIEVTLHPDGSVSVLDNGRGIPVD
IHPKTGQSALVTVMTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSEWLEARVWREGKVYFQRFERGAVVGSLQSEPQPA
GAQRRGTEVRFKPDELIFPEREFDYSLLAARLRELAYLNAGLKIIFSDLRRDPPHREVYQFAGGIKEYVAFINHEKTPIH
PDIIYVRGERDGVEVEAALQWCADVYTDTLIGFANNIRTTEGGTHLEGLKTALTLTINSQARKLNKLKDSDSNLAGENVR
EGLTAILAVKVPNPEFEGQTKTKLGNTEVRGIVQTLVSEALTEYLEFNPNVARGIVEKAIQSFNAAEAARRARELVRRKS
ALESSTLPGKLADCSCRDPAESEVYLVEGDSAGGSAKQGRDRQFQAVLPLRGKILNIEKADPAKIYKNAEIQAMITALGL
GVKGEEFDPSQLRYHRIILMTDADVDGSHIRTLLLTFFYRYQKALLEQGYVYIACPPLYKIEWGRNNVRYCYNEAEKEQV
LRSLPANTKYTIQRFKGLGEMMPEQLWETTMNPATRVLKQITIEDAAEADRIFTILMGDAVGPRREFIEAHSAQLDLAAL
DI
>Mature_641_residues
TENYGAEQIQVLEGLEAVRRRPGMYIGSTGPKGLHHLVYEVVDNSVDEALAGYCNEIEVTLHPDGSVSVLDNGRGIPVDI
HPKTGQSALVTVMTVLHAGGKFGGGGYKVSGGLHGVGVSVVNALSEWLEARVWREGKVYFQRFERGAVVGSLQSEPQPAG
AQRRGTEVRFKPDELIFPEREFDYSLLAARLRELAYLNAGLKIIFSDLRRDPPHREVYQFAGGIKEYVAFINHEKTPIHP
DIIYVRGERDGVEVEAALQWCADVYTDTLIGFANNIRTTEGGTHLEGLKTALTLTINSQARKLNKLKDSDSNLAGENVRE
GLTAILAVKVPNPEFEGQTKTKLGNTEVRGIVQTLVSEALTEYLEFNPNVARGIVEKAIQSFNAAEAARRARELVRRKSA
LESSTLPGKLADCSCRDPAESEVYLVEGDSAGGSAKQGRDRQFQAVLPLRGKILNIEKADPAKIYKNAEIQAMITALGLG
VKGEEFDPSQLRYHRIILMTDADVDGSHIRTLLLTFFYRYQKALLEQGYVYIACPPLYKIEWGRNNVRYCYNEAEKEQVL
RSLPANTKYTIQRFKGLGEMMPEQLWETTMNPATRVLKQITIEDAAEADRIFTILMGDAVGPRREFIEAHSAQLDLAALD
I

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=625, Percent_Identity=25.12, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI19913408, Length=621, Percent_Identity=25.1207729468599, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI48994957, Length=574, Percent_Identity=52.9616724738676, Blast_Score=562, Evalue=1e-161,
Organism=Escherichia coli, GI1789408, Length=623, Percent_Identity=42.6966292134831, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17535065, Length=567, Percent_Identity=25.3968253968254, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI212645845, Length=547, Percent_Identity=25.5941499085923, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=181, Percent_Identity=30.939226519337, Blast_Score=84, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=643, Percent_Identity=24.7278382581649, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI17136538, Length=564, Percent_Identity=25.886524822695, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71165; Mature: 71034

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENYGAEQIQVLEGLEAVRRRPGMYIGSTGPKGLHHLVYEVVDNSVDEALAGYCNEIEV
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEE
TLHPDGSVSVLDNGRGIPVDIHPKTGQSALVTVMTVLHAGGKFGGGGYKVSGGLHGVGVS
EECCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSEWLEARVWREGKVYFQRFERGAVVGSLQSEPQPAGAQRRGTEVRFKPDELIFPE
HHHHHHHHHHHHHHHCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEECCCCCCCCC
REFDYSLLAARLRELAYLNAGLKIIFSDLRRDPPHREVYQFAGGIKEYVAFINHEKTPIH
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCC
PDIIYVRGERDGVEVEAALQWCADVYTDTLIGFANNIRTTEGGTHLEGLKTALTLTINSQ
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEEEECHH
ARKLNKLKDSDSNLAGENVREGLTAILAVKVPNPEFEGQTKTKLGNTEVRGIVQTLVSEA
HHHHHHHCCCCCCCCCHHHHHHCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHH
LTEYLEFNPNVARGIVEKAIQSFNAAEAARRARELVRRKSALESSTLPGKLADCSCRDPA
HHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ESEVYLVEGDSAGGSAKQGRDRQFQAVLPLRGKILNIEKADPAKIYKNAEIQAMITALGL
CCCEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCHHHHHCCCHHHHHHHHHCC
GVKGEEFDPSQLRYHRIILMTDADVDGSHIRTLLLTFFYRYQKALLEQGYVYIACPPLYK
CCCCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
IEWGRNNVRYCYNEAEKEQVLRSLPANTKYTIQRFKGLGEMMPEQLWETTMNPATRVLKQ
EECCCCCCHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ITIEDAAEADRIFTILMGDAVGPRREFIEAHSAQLDLAALDI
HHHHHHHCHHHEEEEEECCCCCHHHHHHHHHCCCEEEEEECC
>Mature Secondary Structure 
TENYGAEQIQVLEGLEAVRRRPGMYIGSTGPKGLHHLVYEVVDNSVDEALAGYCNEIEV
CCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEE
TLHPDGSVSVLDNGRGIPVDIHPKTGQSALVTVMTVLHAGGKFGGGGYKVSGGLHGVGVS
EECCCCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSEWLEARVWREGKVYFQRFERGAVVGSLQSEPQPAGAQRRGTEVRFKPDELIFPE
HHHHHHHHHHHHHHHCHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCEEECCCCCCCCC
REFDYSLLAARLRELAYLNAGLKIIFSDLRRDPPHREVYQFAGGIKEYVAFINHEKTPIH
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCC
PDIIYVRGERDGVEVEAALQWCADVYTDTLIGFANNIRTTEGGTHLEGLKTALTLTINSQ
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEEEECHH
ARKLNKLKDSDSNLAGENVREGLTAILAVKVPNPEFEGQTKTKLGNTEVRGIVQTLVSEA
HHHHHHHCCCCCCCCCHHHHHHCEEEEEEECCCCCCCCCCHHCCCCHHHHHHHHHHHHHH
LTEYLEFNPNVARGIVEKAIQSFNAAEAARRARELVRRKSALESSTLPGKLADCSCRDPA
HHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
ESEVYLVEGDSAGGSAKQGRDRQFQAVLPLRGKILNIEKADPAKIYKNAEIQAMITALGL
CCCEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEECCCCCHHHHHCCCHHHHHHHHHCC
GVKGEEFDPSQLRYHRIILMTDADVDGSHIRTLLLTFFYRYQKALLEQGYVYIACPPLYK
CCCCCCCCHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEE
IEWGRNNVRYCYNEAEKEQVLRSLPANTKYTIQRFKGLGEMMPEQLWETTMNPATRVLKQ
EECCCCCCHHHHCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
ITIEDAAEADRIFTILMGDAVGPRREFIEAHSAQLDLAALDI
HHHHHHHCHHHEEEEEECCCCCHHHHHHHHHCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]