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The map label for this gene is amt

Identifier: 86606173

GI number: 86606173

Start: 1515231

End: 1516637

Strand: Reverse

Name: amt

Synonym: CYA_1507

Alternate gene names: 86606173

Gene position: 1516637-1515231 (Counterclockwise)

Preceding gene: 86606174

Following gene: 86606172

Centisome position: 51.71

GC content: 57.92

Gene sequence:

>1407_bases
ATGAGTACAACAATGATCCAAAAGCTCAAGAGCCAGGTGCCCCAGTGGCTCTGGCGACCGGCCAGGTGGAGTCTGACTGC
TGCTCTAATGGCTGCGCTGGTCTTTCTATTCGGCCAGGGAACTGCCTTTGCTCAAGAGGCCGAGGCAGTTCAGAAACACG
CTGACATTGTTTGGACGATCGTGGCAGCAGCCTTGGTGTTCTTCATGCAGGCGGGGTTCGCCATGGTGGAGACGGGCTTT
ACCCGCGCCAAGAACGCCGCCAACATCATGATGAAGAACTTGATGGACTTCTGCATGGGCTGTTGCGCCTTCTGGTTCAT
CGGGTTTGGCCTCATGTTCGGCAAAAGTACTGGCTTCTTCGGCACTGACTGGTTTGCTTTTGGCTGGCAGCAGGCTTACG
AGGCAGATGGCTGGCCCTTTACTTTCTTTGTCTTCCAGATGGTGTTTGCCGCTACCGCCGCCACCATTGTTTCCGGAGCG
ATGGCAGAGCGCACCAAGTTCTTGGCCTACTTGATCTACTCGCTCTTTATCTCCGCCATCATCTACCCCATATCCGGGGG
GTGGGCTTGGAACGGCCTGTTTGGGGACTACAACGGCGGCACAGTTGGCTGGCTGGAAAATCTGGGCTACATTGATTTCG
CTGGATCGGGGGTGGTGCATTTGGTAGGCGGCGCTGCGGCCTTGGCAGGCGCCATTGTGCTCGGCCCTCGCATTGGCAAG
TACGGCCCTAATGGAGAACCGCGGGCGATCCCTGGCCACAACCTGCCGCTGGGGATGTTGGGGGTGTTTATCCTCGCCTT
GGGCTGGATTGGGTTCAACGCTGGCTCGACCACTGCTGCGAATGCAGACATTGGCTGGGTTGCCATGAATACGTTGCTAG
CCGCGTCAGCGGGGGCCATTGGGGCGACAATCACCTGCTGGGTGCATTTTGGCAAGCCAGACATGACCTTTGGCGCCAAC
GGGGTGCTGGCGGGCTTGGTGGGCATCACTGCCCCTTGCAATACAGTTCATCCGCTGGGGGCCTTGATCATTGGCTCGGT
GGCCGGAATCCTCGTGGTGCTCTCCGCGGTGATTTTGGATACGACGCTGAAAATTGATGACCCAGTGGGCGCGGTTTCCG
TGCATGGCACCTGTGGTATCTGGGGGGTGTTGGCAGCAGGGATCCCTTGGTTTGCCCACGCCAATTCAGAGGTTACCTGG
GCACAGTTCGGCGTGCAGGTGGTAGGCGCTCTCGTTCTCTTCTTCTGGCCCTTCTTCACCTGTCTAATCCTGTTTTTAAT
CCTCAAGGTAACTGTTGGCTTGCGCGTCAGCGCCGAGGAAGAGATTGCCGGTTTGGATGTGGGCGAGCACGGCAACATAG
CCTACCCTGACTTCGTTACTGCTGCCACCGAGGTGGAGGGAGCTTGA

Upstream 100 bases:

>100_bases
CTGCATGTCTCTTTTGCTGTATCTCTGATGAGGTGTTCTTGCCCCACTTTCCGGGTAACGTCTTTTGAGAAGTACTGTCC
CTTTAGGTGAGTCCAAAAGC

Downstream 100 bases:

>100_bases
GCTGTTGCGCTCGGTTCGACGGGAGGCTGTCGGATCTGAGCGACACAGGACTGAAAATCAAGAGAGGGGGTCAGGTCTGG
CCCTCTCTTTTGTGGCTTGT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSTTMIQKLKSQVPQWLWRPARWSLTAALMAALVFLFGQGTAFAQEAEAVQKHADIVWTIVAAALVFFMQAGFAMVETGF
TRAKNAANIMMKNLMDFCMGCCAFWFIGFGLMFGKSTGFFGTDWFAFGWQQAYEADGWPFTFFVFQMVFAATAATIVSGA
MAERTKFLAYLIYSLFISAIIYPISGGWAWNGLFGDYNGGTVGWLENLGYIDFAGSGVVHLVGGAAALAGAIVLGPRIGK
YGPNGEPRAIPGHNLPLGMLGVFILALGWIGFNAGSTTAANADIGWVAMNTLLAASAGAIGATITCWVHFGKPDMTFGAN
GVLAGLVGITAPCNTVHPLGALIIGSVAGILVVLSAVILDTTLKIDDPVGAVSVHGTCGIWGVLAAGIPWFAHANSEVTW
AQFGVQVVGALVLFFWPFFTCLILFLILKVTVGLRVSAEEEIAGLDVGEHGNIAYPDFVTAATEVEGA

Sequences:

>Translated_468_residues
MSTTMIQKLKSQVPQWLWRPARWSLTAALMAALVFLFGQGTAFAQEAEAVQKHADIVWTIVAAALVFFMQAGFAMVETGF
TRAKNAANIMMKNLMDFCMGCCAFWFIGFGLMFGKSTGFFGTDWFAFGWQQAYEADGWPFTFFVFQMVFAATAATIVSGA
MAERTKFLAYLIYSLFISAIIYPISGGWAWNGLFGDYNGGTVGWLENLGYIDFAGSGVVHLVGGAAALAGAIVLGPRIGK
YGPNGEPRAIPGHNLPLGMLGVFILALGWIGFNAGSTTAANADIGWVAMNTLLAASAGAIGATITCWVHFGKPDMTFGAN
GVLAGLVGITAPCNTVHPLGALIIGSVAGILVVLSAVILDTTLKIDDPVGAVSVHGTCGIWGVLAAGIPWFAHANSEVTW
AQFGVQVVGALVLFFWPFFTCLILFLILKVTVGLRVSAEEEIAGLDVGEHGNIAYPDFVTAATEVEGA
>Mature_467_residues
STTMIQKLKSQVPQWLWRPARWSLTAALMAALVFLFGQGTAFAQEAEAVQKHADIVWTIVAAALVFFMQAGFAMVETGFT
RAKNAANIMMKNLMDFCMGCCAFWFIGFGLMFGKSTGFFGTDWFAFGWQQAYEADGWPFTFFVFQMVFAATAATIVSGAM
AERTKFLAYLIYSLFISAIIYPISGGWAWNGLFGDYNGGTVGWLENLGYIDFAGSGVVHLVGGAAALAGAIVLGPRIGKY
GPNGEPRAIPGHNLPLGMLGVFILALGWIGFNAGSTTAANADIGWVAMNTLLAASAGAIGATITCWVHFGKPDMTFGANG
VLAGLVGITAPCNTVHPLGALIIGSVAGILVVLSAVILDTTLKIDDPVGAVSVHGTCGIWGVLAAGIPWFAHANSEVTWA
QFGVQVVGALVLFFWPFFTCLILFLILKVTVGLRVSAEEEIAGLDVGEHGNIAYPDFVTAATEVEGA

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=420, Percent_Identity=34.0476190476191, Blast_Score=187, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17550180, Length=419, Percent_Identity=38.9021479713604, Blast_Score=233, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17550182, Length=417, Percent_Identity=37.410071942446, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI32564042, Length=455, Percent_Identity=32.0879120879121, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI133931100, Length=446, Percent_Identity=30.7174887892377, Blast_Score=178, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6324187, Length=429, Percent_Identity=30.0699300699301, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6325396, Length=438, Percent_Identity=26.9406392694064, Blast_Score=132, Evalue=9e-32,
Organism=Saccharomyces cerevisiae, GI6321559, Length=430, Percent_Identity=28.3720930232558, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI161078314, Length=417, Percent_Identity=36.9304556354916, Blast_Score=228, Evalue=9e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 49483; Mature: 49352

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTMIQKLKSQVPQWLWRPARWSLTAALMAALVFLFGQGTAFAQEAEAVQKHADIVWTI
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VAAALVFFMQAGFAMVETGFTRAKNAANIMMKNLMDFCMGCCAFWFIGFGLMFGKSTGFF
HHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GTDWFAFGWQQAYEADGWPFTFFVFQMVFAATAATIVSGAMAERTKFLAYLIYSLFISAI
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYPISGGWAWNGLFGDYNGGTVGWLENLGYIDFAGSGVVHLVGGAAALAGAIVLGPRIGK
HHCCCCCEEECCCCCCCCCCEEHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHCCCCCCC
YGPNGEPRAIPGHNLPLGMLGVFILALGWIGFNAGSTTAANADIGWVAMNTLLAASAGAI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
GATITCWVHFGKPDMTFGANGVLAGLVGITAPCNTVHPLGALIIGSVAGILVVLSAVILD
CCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TTLKIDDPVGAVSVHGTCGIWGVLAAGIPWFAHANSEVTWAQFGVQVVGALVLFFWPFFT
HHEEECCCCCEEEECCCHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
CLILFLILKVTVGLRVSAEEEIAGLDVGEHGNIAYPDFVTAATEVEGA
HHHHHHHHHHHHCCEECCHHHHCCCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
STTMIQKLKSQVPQWLWRPARWSLTAALMAALVFLFGQGTAFAQEAEAVQKHADIVWTI
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VAAALVFFMQAGFAMVETGFTRAKNAANIMMKNLMDFCMGCCAFWFIGFGLMFGKSTGFF
HHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GTDWFAFGWQQAYEADGWPFTFFVFQMVFAATAATIVSGAMAERTKFLAYLIYSLFISAI
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYPISGGWAWNGLFGDYNGGTVGWLENLGYIDFAGSGVVHLVGGAAALAGAIVLGPRIGK
HHCCCCCEEECCCCCCCCCCEEHHHHCCCCEEECCCCCEEEHHHHHHHHHHHHCCCCCCC
YGPNGEPRAIPGHNLPLGMLGVFILALGWIGFNAGSTTAANADIGWVAMNTLLAASAGAI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
GATITCWVHFGKPDMTFGANGVLAGLVGITAPCNTVHPLGALIIGSVAGILVVLSAVILD
CCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TTLKIDDPVGAVSVHGTCGIWGVLAAGIPWFAHANSEVTWAQFGVQVVGALVLFFWPFFT
HHEEECCCCCEEEECCCHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
CLILFLILKVTVGLRVSAEEEIAGLDVGEHGNIAYPDFVTAATEVEGA
HHHHHHHHHHHHCCEECCHHHHCCCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]