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The map label for this gene is dapF

Identifier: 86606001

GI number: 86606001

Start: 1340188

End: 1341045

Strand: Reverse

Name: dapF

Synonym: CYA_1324

Alternate gene names: 86606001

Gene position: 1341045-1340188 (Counterclockwise)

Preceding gene: 86606002

Following gene: 86605998

Centisome position: 45.73

GC content: 65.62

Gene sequence:

>858_bases
TTGCCCTCCCGTCTCTCCTTCCACAAATACCAGGGGCTGGGGAACGACTTTATCTTGGTGGACAACCGCCACCAGCCGCA
GCCCTGTCTGACGCCTGAGGAAGCCGTTGCCCTCTGCAACCGCCGCTTTGGCGTGGGGGCCGATGGGGTTATCTTTCTGC
TGCCGGGGCAGGAGGGGGCCGACTTCAGCATGCGCCTGTTCAACAGCGACGGCTCGGAGGCGGAGATGTGCGGCAACGGC
ATCCGCTGTTTGGCCCGCTTTTTGCAGGATCTGGGGATCCCTGGCCAGGACGGCGCCTACCAGATCCACACCTTGGCGGG
GCGGATCGTGCCGCAGGTGCGGCCCGATGGCTTGGTAACAGTGGACATGGGGATCCCTCGCCTCTTGGCCGGGCAGATCC
CCACCACGCTGGCCAAACCAGAGGAAAAGGTGGTGCGGCAGCCCCTGCAGGTAGGCGGGCGGGAGTGGGCGGTGACGGCG
GTGAGCATGGGCAACCCCCACTGTGTGGTCTTTTTGGAAGAAGGGGGATCCCTGGAAGAGCTGGACTTGGAAAGGGTGGG
CCCCCTGTTTGAGCATCACCCGGCTTTTCCCGAGCGCACCAACACCGAATTTGCCCAGGTGCTCGGCCCAAATTACCTGC
GGCTGCGGGTTTGGGAGCGGGGGGCAGGGGTGACGCTGGCCTGTGGGACAGGCGCCTGTGCCGCCTTGGTGGCCGCCGTT
TTGGAAGAGCGGGCCGAGCCGCAAGCCACGGTGGAGCTGCCGGGAGGCAATCTGGAGATTCGCTGGGATCCCGATACCCA
ACACGTGTGGATGACCGGGCCGGCCCTGCCCGTTTTCAGCGGCACCACGGCCGAGTGA

Upstream 100 bases:

>100_bases
ACACCGCCTTTGGCCCCCCTTCCAAAAACTTGCAGGATCCCTACGAGATGCACGAGGATTGAAGCCGGTTGTTGTGCGGT
TCCGCTGCATGCCTGTTCGG

Downstream 100 bases:

>100_bases
GAGATCTCAGGATCCCAAACCCAGCAAGAGAAAGGCGAGCAGGGCGAGGATCCCGCGGGCCACGCTCCAACCTTGACGCC
AGATCAGCGCCACCGCCAGG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MPSRLSFHKYQGLGNDFILVDNRHQPQPCLTPEEAVALCNRRFGVGADGVIFLLPGQEGADFSMRLFNSDGSEAEMCGNG
IRCLARFLQDLGIPGQDGAYQIHTLAGRIVPQVRPDGLVTVDMGIPRLLAGQIPTTLAKPEEKVVRQPLQVGGREWAVTA
VSMGNPHCVVFLEEGGSLEELDLERVGPLFEHHPAFPERTNTEFAQVLGPNYLRLRVWERGAGVTLACGTGACAALVAAV
LEERAEPQATVELPGGNLEIRWDPDTQHVWMTGPALPVFSGTTAE

Sequences:

>Translated_285_residues
MPSRLSFHKYQGLGNDFILVDNRHQPQPCLTPEEAVALCNRRFGVGADGVIFLLPGQEGADFSMRLFNSDGSEAEMCGNG
IRCLARFLQDLGIPGQDGAYQIHTLAGRIVPQVRPDGLVTVDMGIPRLLAGQIPTTLAKPEEKVVRQPLQVGGREWAVTA
VSMGNPHCVVFLEEGGSLEELDLERVGPLFEHHPAFPERTNTEFAQVLGPNYLRLRVWERGAGVTLACGTGACAALVAAV
LEERAEPQATVELPGGNLEIRWDPDTQHVWMTGPALPVFSGTTAE
>Mature_284_residues
PSRLSFHKYQGLGNDFILVDNRHQPQPCLTPEEAVALCNRRFGVGADGVIFLLPGQEGADFSMRLFNSDGSEAEMCGNGI
RCLARFLQDLGIPGQDGAYQIHTLAGRIVPQVRPDGLVTVDMGIPRLLAGQIPTTLAKPEEKVVRQPLQVGGREWAVTAV
SMGNPHCVVFLEEGGSLEELDLERVGPLFEHHPAFPERTNTEFAQVLGPNYLRLRVWERGAGVTLACGTGACAALVAAVL
EERAEPQATVELPGGNLEIRWDPDTQHVWMTGPALPVFSGTTAE

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=278, Percent_Identity=39.568345323741, Blast_Score=194, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_SYNJA (Q2JUV6)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_474764.1
- HSSP:   P44859
- ProteinModelPortal:   Q2JUV6
- SMR:   Q2JUV6
- STRING:   Q2JUV6
- GeneID:   3899492
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_1324
- TIGR:   CYA_1324
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   SSP321327:CYA_1324-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30870; Mature: 30738

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 77-77 ACT_SITE 228-228

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRLSFHKYQGLGNDFILVDNRHQPQPCLTPEEAVALCNRRFGVGADGVIFLLPGQEGA
CCCCCCCHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCC
DFSMRLFNSDGSEAEMCGNGIRCLARFLQDLGIPGQDGAYQIHTLAGRIVPQVRPDGLVT
CEEEEEECCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCCCCCCEEE
VDMGIPRLLAGQIPTTLAKPEEKVVRQPLQVGGREWAVTAVSMGNPHCVVFLEEGGSLEE
ECCCCCHHHHCCCCCHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCCC
LDLERVGPLFEHHPAFPERTNTEFAQVLGPNYLRLRVWERGAGVTLACGTGACAALVAAV
CCHHHHCCHHHCCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCEEEEECCCHHHHHHHHH
LEERAEPQATVELPGGNLEIRWDPDTQHVWMTGPALPVFSGTTAE
HHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCCCEECCCCCCC
>Mature Secondary Structure 
PSRLSFHKYQGLGNDFILVDNRHQPQPCLTPEEAVALCNRRFGVGADGVIFLLPGQEGA
CCCCCCHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCC
DFSMRLFNSDGSEAEMCGNGIRCLARFLQDLGIPGQDGAYQIHTLAGRIVPQVRPDGLVT
CEEEEEECCCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHCCCCCCCCEEE
VDMGIPRLLAGQIPTTLAKPEEKVVRQPLQVGGREWAVTAVSMGNPHCVVFLEEGGSLEE
ECCCCCHHHHCCCCCHHCCCHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCCCC
LDLERVGPLFEHHPAFPERTNTEFAQVLGPNYLRLRVWERGAGVTLACGTGACAALVAAV
CCHHHHCCHHHCCCCCCCCCCCHHHHHHCCCEEEEEEEECCCCEEEEECCCHHHHHHHHH
LEERAEPQATVELPGGNLEIRWDPDTQHVWMTGPALPVFSGTTAE
HHHHCCCCEEEEECCCEEEEEECCCCCEEEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA