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The map label for this gene is pcrA

Identifier: 86605998

GI number: 86605998

Start: 1336315

End: 1338645

Strand: Reverse

Name: pcrA

Synonym: CYA_1321

Alternate gene names: 86605998

Gene position: 1338645-1336315 (Counterclockwise)

Preceding gene: 86606001

Following gene: 86605997

Centisome position: 45.64

GC content: 56.54

Gene sequence:

>2331_bases
TTGTTGGATGCCCTCAACCCTGCTCAACGGCAAGCGGTGCACCATTTTTGTGGCCCTTTGCTGGTGGTGGCGGGGGCAGG
TTCCGGCAAGACGCGCACCCTCGCCTATCGCATCGCCCACCTCATCCGCCACTACCGGGTGGATCCGGAGGAGATTTTGG
CCGTGACCTTTACCAACAAGGCCGCCCGTGAGATGAAAGAGCGCATTGAGCAGCTCTTTGCCGAACAAGAAGCTCAAGAA
CAATTTGGGATCCCCTTAGAGGAATTGGAGCCGGCCCAGGCAACGCGACTAAAATCTGCTGTTTACCACCGCTGGATTAA
GCCTCTTTGGATTGGCACGTTCCACAGCTTATTTGCCCAGATCCTGCGCCTGGAGATCGAAAAATATCAGGATCCCAAGG
GACGAAAGTGGACTCGCAACTTCTCGATTTTCGATGAATCGGACGCCCAATCCCTGGTTAAAGAGATTGTAACTAAGCAA
CTCAACTTAGATGAGCGCAAGTTCGATCCAAGATCGGTGCGCTATGCCATTAGCAATGCCAAAAATCAAGGTTGGACCCC
TGCCGATCTGGAGCGCCACCAGCCCAATTTCCGAGGTCGCATCCTGGCTCAGGTTTACGAGCTCTACGAAGATCAACTGG
CGGCCAATAATGCCCTCGATTTCGACAACTTAATTTGGATCCCGGTGCAACTTTTCCGCCAAAATGAGCAGGTTTTGGCC
TACTGGCACCAGCGCTTTCGGCACATCTTGGTGGACGAATACCAAGATACCAACCGCACCCAGTACGAGCTGATCCGGCT
CCTGGCCACCAACGGCGAGACCCGCCGCTCCGAGTGGGACTGGACCAATCGCTCCCTCTTTGTGGTGGGCGATGCAGATC
AAAGCATCTATCGCTTTCGGGGGGCCGATTTCACCATTTTGCTGGAGTTTCAAGAGACCTTTGGCGATGGCCTGCCGGAC
GACGATACCCGCACCCTGATCAAATTGGAAGAAAACTATCGCTCCACCGCCACGATTTTGCAGGCGGCTAACGTCCTGAT
TGAGAAAAACACCGAGCGCATCGATAAAATTCTCCGGCCAACTCGCCCCCCCGGCCATCCCATCGTTTGCTACCAGGCGG
AGGACGAAATGGCCGAGGCCGAGTTTGTGGTCAATACCATCCGGCGATTACACAGCCAAAATCCCAACTCTTCCTGGGGA
GATTTTGCCATTCTCTACCGCACCAATGCCCAATCTCGCCCTTTTGAAGAGGTTTTGCTGCGGTGGGGGATCCCTTACAC
GGTGGTGGGGGGGCTGCGATTTTACGATCGCAAAGAGATTAAGGATGTGCTGGCCTATTTGCGGGTTGTGCTCAACCCTG
CCGATTCTTTTAGCCTGTTGCGCATTATCAATGTGCCGCGGCGGGGCATCGGCAAGTCCACTCTGGACAAGCTAAGCGAA
GCGGCGGCTGCCTTAAATGTGCCCCTGTGGACTATCCTCAGCGACGAGGCCTCTGTTACCAACCTGCTGGGGCGTTCGGC
CAAGCCGGTTCTCCAGTTTGCCCAGCTTATCCAGCATTGGCACAGACAGATGGCCCAGGTTTCGGCTGCCGCCCTTCTGG
AGGGGCTGCTGCGGGATTGCGGCTATCTGGAAGATCTCAAGGCCCAGGGCACAGAAGAGGCCGAGGAAAGGATCGCCAAC
GTGATGGAACTCTACAATGCCGCCCGCCAATTTGAAGAAGAACAGGAGGATCCCAGCCTCGAGGCTTTCTTGAGCAATGT
GTCTTTAGCTTCCGATTTGGACACCCTTCAGGAGGGAGCTGAGAAAGTTTCGCTAATGACGTTGCATGCTTCCAAAGGGC
TGGAGTTCCCGGTGGTGTTTTTGGTGGGGTTGGAACAAGGCCTGTTTCCCAATTTTCGCGCTTTGGAGGATCCTGCCGCG
CTGGAAGAAGAACGGCGCCTCTGCTACGTGGGCATCACCCGCGCCCGCGAGCAATTGTTTGTCTGCCACGCCAGCGAGCG
GCGGCTCTACGGCAACCGCGAGCCCGCCATCCCTTCCCTATTTTTGAGTGAGCTGCCCCAAGAGCTGATCCAATGGGAAA
AAGGCAGGGGGCTTTCGTTCTCCGCCGACAACACGGCTGCGGCCACGCCCAAAGCAAAGGCTGCTCGCCGCCAAGGCCGT
TCCTCCCAGTGGGCCGTTGGGGATCGCCTGCAACATCCCCAATTCGGACAGGGGCAAATTACCCACGTGTTCGGCAGCGG
CGAACGGATCACCCTGGCGGTGAGGTTTCCCGCCATCGGCCAGCAGAAAATCTTGGATCCCCGCATCGCGCCCATTCAAA
AGCTCAGCTAG

Upstream 100 bases:

>100_bases
CTTTGGTGCTCCAAAATGCCCTCTGGGCTTCTCATCCCCATTGGGTTGGCGCCCATAATGGAAAGCAGTTTCTGCTTTGC
CGACTATGACTCACGCCTGT

Downstream 100 bases:

>100_bases
CTGGCTGGGCTCGAAACCGCTTTGGAGATGGATGGCGTAGCGGTATGGGTCTCATCCTTTTTTGTAGGTGCCTTGATCTC
GCCCAGAACTTGCTTGGAAT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 776; Mature: 776

Protein sequence:

>776_residues
MLDALNPAQRQAVHHFCGPLLVVAGAGSGKTRTLAYRIAHLIRHYRVDPEEILAVTFTNKAAREMKERIEQLFAEQEAQE
QFGIPLEELEPAQATRLKSAVYHRWIKPLWIGTFHSLFAQILRLEIEKYQDPKGRKWTRNFSIFDESDAQSLVKEIVTKQ
LNLDERKFDPRSVRYAISNAKNQGWTPADLERHQPNFRGRILAQVYELYEDQLAANNALDFDNLIWIPVQLFRQNEQVLA
YWHQRFRHILVDEYQDTNRTQYELIRLLATNGETRRSEWDWTNRSLFVVGDADQSIYRFRGADFTILLEFQETFGDGLPD
DDTRTLIKLEENYRSTATILQAANVLIEKNTERIDKILRPTRPPGHPIVCYQAEDEMAEAEFVVNTIRRLHSQNPNSSWG
DFAILYRTNAQSRPFEEVLLRWGIPYTVVGGLRFYDRKEIKDVLAYLRVVLNPADSFSLLRIINVPRRGIGKSTLDKLSE
AAAALNVPLWTILSDEASVTNLLGRSAKPVLQFAQLIQHWHRQMAQVSAAALLEGLLRDCGYLEDLKAQGTEEAEERIAN
VMELYNAARQFEEEQEDPSLEAFLSNVSLASDLDTLQEGAEKVSLMTLHASKGLEFPVVFLVGLEQGLFPNFRALEDPAA
LEEERRLCYVGITRAREQLFVCHASERRLYGNREPAIPSLFLSELPQELIQWEKGRGLSFSADNTAAATPKAKAARRQGR
SSQWAVGDRLQHPQFGQGQITHVFGSGERITLAVRFPAIGQQKILDPRIAPIQKLS

Sequences:

>Translated_776_residues
MLDALNPAQRQAVHHFCGPLLVVAGAGSGKTRTLAYRIAHLIRHYRVDPEEILAVTFTNKAAREMKERIEQLFAEQEAQE
QFGIPLEELEPAQATRLKSAVYHRWIKPLWIGTFHSLFAQILRLEIEKYQDPKGRKWTRNFSIFDESDAQSLVKEIVTKQ
LNLDERKFDPRSVRYAISNAKNQGWTPADLERHQPNFRGRILAQVYELYEDQLAANNALDFDNLIWIPVQLFRQNEQVLA
YWHQRFRHILVDEYQDTNRTQYELIRLLATNGETRRSEWDWTNRSLFVVGDADQSIYRFRGADFTILLEFQETFGDGLPD
DDTRTLIKLEENYRSTATILQAANVLIEKNTERIDKILRPTRPPGHPIVCYQAEDEMAEAEFVVNTIRRLHSQNPNSSWG
DFAILYRTNAQSRPFEEVLLRWGIPYTVVGGLRFYDRKEIKDVLAYLRVVLNPADSFSLLRIINVPRRGIGKSTLDKLSE
AAAALNVPLWTILSDEASVTNLLGRSAKPVLQFAQLIQHWHRQMAQVSAAALLEGLLRDCGYLEDLKAQGTEEAEERIAN
VMELYNAARQFEEEQEDPSLEAFLSNVSLASDLDTLQEGAEKVSLMTLHASKGLEFPVVFLVGLEQGLFPNFRALEDPAA
LEEERRLCYVGITRAREQLFVCHASERRLYGNREPAIPSLFLSELPQELIQWEKGRGLSFSADNTAAATPKAKAARRQGR
SSQWAVGDRLQHPQFGQGQITHVFGSGERITLAVRFPAIGQQKILDPRIAPIQKLS
>Mature_776_residues
MLDALNPAQRQAVHHFCGPLLVVAGAGSGKTRTLAYRIAHLIRHYRVDPEEILAVTFTNKAAREMKERIEQLFAEQEAQE
QFGIPLEELEPAQATRLKSAVYHRWIKPLWIGTFHSLFAQILRLEIEKYQDPKGRKWTRNFSIFDESDAQSLVKEIVTKQ
LNLDERKFDPRSVRYAISNAKNQGWTPADLERHQPNFRGRILAQVYELYEDQLAANNALDFDNLIWIPVQLFRQNEQVLA
YWHQRFRHILVDEYQDTNRTQYELIRLLATNGETRRSEWDWTNRSLFVVGDADQSIYRFRGADFTILLEFQETFGDGLPD
DDTRTLIKLEENYRSTATILQAANVLIEKNTERIDKILRPTRPPGHPIVCYQAEDEMAEAEFVVNTIRRLHSQNPNSSWG
DFAILYRTNAQSRPFEEVLLRWGIPYTVVGGLRFYDRKEIKDVLAYLRVVLNPADSFSLLRIINVPRRGIGKSTLDKLSE
AAAALNVPLWTILSDEASVTNLLGRSAKPVLQFAQLIQHWHRQMAQVSAAALLEGLLRDCGYLEDLKAQGTEEAEERIAN
VMELYNAARQFEEEQEDPSLEAFLSNVSLASDLDTLQEGAEKVSLMTLHASKGLEFPVVFLVGLEQGLFPNFRALEDPAA
LEEERRLCYVGITRAREQLFVCHASERRLYGNREPAIPSLFLSELPQELIQWEKGRGLSFSADNTAAATPKAKAARRQGR
SSQWAVGDRLQHPQFGQGQITHVFGSGERITLAVRFPAIGQQKILDPRIAPIQKLS

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=764, Percent_Identity=37.303664921466, Blast_Score=457, Evalue=1e-129,
Organism=Escherichia coli, GI48994965, Length=729, Percent_Identity=38.5459533607682, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI1787196, Length=371, Percent_Identity=29.6495956873315, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=778, Percent_Identity=26.9922879177378, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6324477, Length=482, Percent_Identity=25.7261410788382, Blast_Score=120, Evalue=8e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 88713; Mature: 88713

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDALNPAQRQAVHHFCGPLLVVAGAGSGKTRTLAYRIAHLIRHYRVDPEEILAVTFTNK
CCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEECCH
AAREMKERIEQLFAEQEAQEQFGIPLEELEPAQATRLKSAVYHRWIKPLWIGTFHSLFAQ
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILRLEIEKYQDPKGRKWTRNFSIFDESDAQSLVKEIVTKQLNLDERKFDPRSVRYAISNA
HHHHHHHHCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHCC
KNQGWTPADLERHQPNFRGRILAQVYELYEDQLAANNALDFDNLIWIPVQLFRQNEQVLA
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHCCHHHHH
YWHQRFRHILVDEYQDTNRTQYELIRLLATNGETRRSEWDWTNRSLFVVGDADQSIYRFR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHCC
GADFTILLEFQETFGDGLPDDDTRTLIKLEENYRSTATILQAANVLIEKNTERIDKILRP
CCCEEEEEEEHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
TRPPGHPIVCYQAEDEMAEAEFVVNTIRRLHSQNPNSSWGDFAILYRTNAQSRPFEEVLL
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHH
RWGIPYTVVGGLRFYDRKEIKDVLAYLRVVLNPADSFSLLRIINVPRRGIGKSTLDKLSE
HCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHH
AAAALNVPLWTILSDEASVTNLLGRSAKPVLQFAQLIQHWHRQMAQVSAAALLEGLLRDC
HHHHHCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYLEDLKAQGTEEAEERIANVMELYNAARQFEEEQEDPSLEAFLSNVSLASDLDTLQEGA
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHH
EKVSLMTLHASKGLEFPVVFLVGLEQGLFPNFRALEDPAALEEERRLCYVGITRAREQLF
HHEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEEEHHHHHHHEE
VCHASERRLYGNREPAIPSLFLSELPQELIQWEKGRGLSFSADNTAAATPKAKAARRQGR
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHCCC
SSQWAVGDRLQHPQFGQGQITHVFGSGERITLAVRFPAIGQQKILDPRIAPIQKLS
CCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHCCCCCCCCHHCCC
>Mature Secondary Structure
MLDALNPAQRQAVHHFCGPLLVVAGAGSGKTRTLAYRIAHLIRHYRVDPEEILAVTFTNK
CCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEECCH
AAREMKERIEQLFAEQEAQEQFGIPLEELEPAQATRLKSAVYHRWIKPLWIGTFHSLFAQ
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILRLEIEKYQDPKGRKWTRNFSIFDESDAQSLVKEIVTKQLNLDERKFDPRSVRYAISNA
HHHHHHHHCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHCC
KNQGWTPADLERHQPNFRGRILAQVYELYEDQLAANNALDFDNLIWIPVQLFRQNEQVLA
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHCCHHHHH
YWHQRFRHILVDEYQDTNRTQYELIRLLATNGETRRSEWDWTNRSLFVVGDADQSIYRFR
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHCC
GADFTILLEFQETFGDGLPDDDTRTLIKLEENYRSTATILQAANVLIEKNTERIDKILRP
CCCEEEEEEEHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
TRPPGHPIVCYQAEDEMAEAEFVVNTIRRLHSQNPNSSWGDFAILYRTNAQSRPFEEVLL
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHH
RWGIPYTVVGGLRFYDRKEIKDVLAYLRVVLNPADSFSLLRIINVPRRGIGKSTLDKLSE
HCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHH
AAAALNVPLWTILSDEASVTNLLGRSAKPVLQFAQLIQHWHRQMAQVSAAALLEGLLRDC
HHHHHCCCEEEEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GYLEDLKAQGTEEAEERIANVMELYNAARQFEEEQEDPSLEAFLSNVSLASDLDTLQEGA
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHH
EKVSLMTLHASKGLEFPVVFLVGLEQGLFPNFRALEDPAALEEERRLCYVGITRAREQLF
HHEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEEEHHHHHHHEE
VCHASERRLYGNREPAIPSLFLSELPQELIQWEKGRGLSFSADNTAAATPKAKAARRQGR
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCHHHHHHHCCC
SSQWAVGDRLQHPQFGQGQITHVFGSGERITLAVRFPAIGQQKILDPRIAPIQKLS
CCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCCHHCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]