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The map label for this gene is metK

Identifier: 86605873

GI number: 86605873

Start: 1200073

End: 1201317

Strand: Reverse

Name: metK

Synonym: CYA_1186

Alternate gene names: 86605873

Gene position: 1201317-1200073 (Counterclockwise)

Preceding gene: 86605874

Following gene: 86605866

Centisome position: 40.96

GC content: 62.49

Gene sequence:

>1245_bases
TTGTTTTCCTCAGAGTCGGTGACAGAAGGGCACCCCGACAAAATTTGCGACCAGATCTCTGACGCCATCGTGGATGCCCT
CTTGACGGCGGATCCCTTAAGTCGAGTGGCGGCAGAGGTGGTGGTGAATACAGGCATGGTGATCCTGACCGGGGAGATCA
CCTCGCAGGCGCATGTCAACTTCACCCGACTGGTGCGGGACAAGGTGGCCGAAATTGGCTATACCGACGCCAAGAACGGC
TTCTCTGCAGAAAGCTGCGCGGTGTTGGTGGCTTTCGATGAACAATCCCCCGACATCGCCCAGGGGGTGAACTTGGCTCT
GGAAAGTCGCACATCTCAGGAAGATGAATTCGACTTGATCGGGGCCGGGGATCAGGGCTTGATGTTTGGCTTCGCCTGCG
ACGAAACCCCCGAGTTGATGCCACTGCCCATCAGCTTGGCCCACCGCCTCAGCCGCCAGTTGGCCGCGGTTCGCAAAAAC
GGCACCCTGCCCTACCTGCGTCCGGATGGCAAAACGCAGGTGACTGTGGCCTATGAGGAGGGGCGGCCGGTGGGCATTCA
CACCCTGCTGATCTCTACCCAGCACACGCCGACCATCGGTGCCATTACAGAAGAGGCAGCGGTGCAGGAGCGGATCCGCG
CCGATCTCTGGGAGGCGGTGGTGCAGCCGGTGTTTGCCGAGCTGCCCATCAAACCCGATGGCAACACCCGCTTCCTCACC
AATCCCACCGGCAAGTTCGTCATCGGCGGACCCCAGGGGGATGCCGGCCTGACCGGACGCAAGATCATCGTCGATACCTA
CGGCGGCTATTCCCGCCACGGGGGCGGGGCCTTCTCCGGCAAGGATCCCACCAAGGTGGATCGCAGCGCCGCCTATGCCG
CCCGCTATGTGGCCAAGAACATCGTGGCCGCCGGCCTGGCGCAAAAATGCGAGGTGCAGGTCAGCTACGCCATTGGGGTG
GCTCGTCCCATCAACCTTTTGGTGGAAACTTTTGGCACCGGCCGCATCCCCGATGAAGCTCTGCTGCGGCTGGTGCAGCG
CCACTTCGACCTGAGGCCGGCGGCCATTCTGGCGCAGTTCCAACTGCGAGAGTTGCCCCGCCAAAGGGGAGGGCGCTTCT
ATCAAAACGTGGCGGTCTACGGGCACTTCGGGCAAACCCATCTGGATCTGCCCTGGGAGCGCACGGATAAGGCTGCCCTG
CTGCGGGAGGAAGCTTTCGCCGGGGCGACCGCCATCCTAGGCTAG

Upstream 100 bases:

>100_bases
GGCCGGCGGATGATGAACGGCAGCAGCAGGCTCTGCTTTCTGGGCAGTTCTTGGTTGTATCTGTTTCGTCTTAAGGAGAG
GTTCGTTGTCCAAGTCGTTT

Downstream 100 bases:

>100_bases
GGGGATCAAGGGGGACAGGCTGGGCTCAATGCCTGCCCTGGTGCAGTTGCTCGACAAGTGGGATAGCTCCGGCAGGATAG
GCTGTGCACAGCCAGTTCAC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MFSSESVTEGHPDKICDQISDAIVDALLTADPLSRVAAEVVVNTGMVILTGEITSQAHVNFTRLVRDKVAEIGYTDAKNG
FSAESCAVLVAFDEQSPDIAQGVNLALESRTSQEDEFDLIGAGDQGLMFGFACDETPELMPLPISLAHRLSRQLAAVRKN
GTLPYLRPDGKTQVTVAYEEGRPVGIHTLLISTQHTPTIGAITEEAAVQERIRADLWEAVVQPVFAELPIKPDGNTRFLT
NPTGKFVIGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLAQKCEVQVSYAIGV
ARPINLLVETFGTGRIPDEALLRLVQRHFDLRPAAILAQFQLRELPRQRGGRFYQNVAVYGHFGQTHLDLPWERTDKAAL
LREEAFAGATAILG

Sequences:

>Translated_414_residues
MFSSESVTEGHPDKICDQISDAIVDALLTADPLSRVAAEVVVNTGMVILTGEITSQAHVNFTRLVRDKVAEIGYTDAKNG
FSAESCAVLVAFDEQSPDIAQGVNLALESRTSQEDEFDLIGAGDQGLMFGFACDETPELMPLPISLAHRLSRQLAAVRKN
GTLPYLRPDGKTQVTVAYEEGRPVGIHTLLISTQHTPTIGAITEEAAVQERIRADLWEAVVQPVFAELPIKPDGNTRFLT
NPTGKFVIGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLAQKCEVQVSYAIGV
ARPINLLVETFGTGRIPDEALLRLVQRHFDLRPAAILAQFQLRELPRQRGGRFYQNVAVYGHFGQTHLDLPWERTDKAAL
LREEAFAGATAILG
>Mature_414_residues
MFSSESVTEGHPDKICDQISDAIVDALLTADPLSRVAAEVVVNTGMVILTGEITSQAHVNFTRLVRDKVAEIGYTDAKNG
FSAESCAVLVAFDEQSPDIAQGVNLALESRTSQEDEFDLIGAGDQGLMFGFACDETPELMPLPISLAHRLSRQLAAVRKN
GTLPYLRPDGKTQVTVAYEEGRPVGIHTLLISTQHTPTIGAITEEAAVQERIRADLWEAVVQPVFAELPIKPDGNTRFLT
NPTGKFVIGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKNIVAAGLAQKCEVQVSYAIGV
ARPINLLVETFGTGRIPDEALLRLVQRHFDLRPAAILAQFQLRELPRQRGGRFYQNVAVYGHFGQTHLDLPWERTDKAAL
LREEAFAGATAILG

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=398, Percent_Identity=53.5175879396985, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI4557737, Length=398, Percent_Identity=54.0201005025126, Blast_Score=386, Evalue=1e-107,
Organism=Escherichia coli, GI1789311, Length=408, Percent_Identity=56.3725490196078, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17538498, Length=398, Percent_Identity=52.0100502512563, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25145633, Length=407, Percent_Identity=50.6142506142506, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17538494, Length=398, Percent_Identity=51.5075376884422, Blast_Score=383, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17551082, Length=398, Percent_Identity=50.5025125628141, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565911, Length=353, Percent_Identity=50.4249291784703, Blast_Score=322, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25145635, Length=362, Percent_Identity=48.8950276243094, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI32565905, Length=398, Percent_Identity=46.2311557788945, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI32565903, Length=326, Percent_Identity=50, Blast_Score=290, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI32565909, Length=81, Percent_Identity=56.7901234567901, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323209, Length=398, Percent_Identity=50.5025125628141, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6320710, Length=401, Percent_Identity=50.6234413965087, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45552159, Length=398, Percent_Identity=50.5025125628141, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580529, Length=398, Percent_Identity=50.5025125628141, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580537, Length=398, Percent_Identity=50.5025125628141, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580533, Length=398, Percent_Identity=50.5025125628141, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580535, Length=398, Percent_Identity=50.5025125628141, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580531, Length=398, Percent_Identity=50.5025125628141, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580527, Length=398, Percent_Identity=50, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580525, Length=398, Percent_Identity=50, Blast_Score=352, Evalue=3e-97,
Organism=Drosophila melanogaster, GI24580541, Length=400, Percent_Identity=48.5, Blast_Score=334, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=52.8, Blast_Score=131, Evalue=7e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_SYNJA (Q2JV74)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_474636.1
- ProteinModelPortal:   Q2JV74
- SMR:   Q2JV74
- STRING:   Q2JV74
- GeneID:   3898895
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_1186
- TIGR:   CYA_1186
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- ProtClustDB:   PRK05250
- BioCyc:   SSP321327:CYA_1186-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44707; Mature: 44707

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSSESVTEGHPDKICDQISDAIVDALLTADPLSRVAAEVVVNTGMVILTGEITSQAHVN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHH
FTRLVRDKVAEIGYTDAKNGFSAESCAVLVAFDEQSPDIAQGVNLALESRTSQEDEFDLI
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHCCCCEECCCCCCCCCEEEE
GAGDQGLMFGFACDETPELMPLPISLAHRLSRQLAAVRKNGTLPYLRPDGKTQVTVAYEE
ECCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEEC
GRPVGIHTLLISTQHTPTIGAITEEAAVQERIRADLWEAVVQPVFAELPIKPDGNTRFLT
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
NPTGKFVIGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKN
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IVAAGLAQKCEVQVSYAIGVARPINLLVETFGTGRIPDEALLRLVQRHFDLRPAAILAQF
HHHHCCHHHCEEEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
QLRELPRQRGGRFYQNVAVYGHFGQTHLDLPWERTDKAALLREEAFAGATAILG
HHHHHHHHHCCCEEEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MFSSESVTEGHPDKICDQISDAIVDALLTADPLSRVAAEVVVNTGMVILTGEITSQAHVN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHH
FTRLVRDKVAEIGYTDAKNGFSAESCAVLVAFDEQSPDIAQGVNLALESRTSQEDEFDLI
HHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCHHHCCCCEECCCCCCCCCEEEE
GAGDQGLMFGFACDETPELMPLPISLAHRLSRQLAAVRKNGTLPYLRPDGKTQVTVAYEE
ECCCCCEEEEEECCCCCCCEECCHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEEEEEEC
GRPVGIHTLLISTQHTPTIGAITEEAAVQERIRADLWEAVVQPVFAELPIKPDGNTRFLT
CCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
NPTGKFVIGGPQGDAGLTGRKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYVAKN
CCCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IVAAGLAQKCEVQVSYAIGVARPINLLVETFGTGRIPDEALLRLVQRHFDLRPAAILAQF
HHHHCCHHHCEEEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
QLRELPRQRGGRFYQNVAVYGHFGQTHLDLPWERTDKAALLREEAFAGATAILG
HHHHHHHHHCCCEEEEEEEEEECCCEEECCCCCCCCHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA