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The map label for this gene is ispH

Identifier: 86605836

GI number: 86605836

Start: 1171869

End: 1173149

Strand: Reverse

Name: ispH

Synonym: CYA_1148

Alternate gene names: 86605836

Gene position: 1173149-1171869 (Counterclockwise)

Preceding gene: 86605837

Following gene: 86605835

Centisome position: 40.0

GC content: 60.89

Gene sequence:

>1281_bases
ATGCCTCATTCCCAACCCTCTTCCCCGGCTAGCTCCCCCCAGGATCCGCGCGCCCTGCGCAAGGCCCTGCGCAGCTCCAG
CAACTACTTTGCCAAAGGGTTTGAAGAGCACAAGGATCAGGCCCAACGGGAGCTGCAAACCACCTACAAAAGCCCCCTGG
TGGAGCAGATCCGCGCCCAGTCCTACTGCTATCGGCAGGGGGATATCACCATCCATCTGGCCAAGGCCTTTGGCTTCTGC
TGGGGGGTGGAACGGGCGGTGGAATATGCCTACGAAGCGCGGCGCCGCTTTCCGGATCGCCAGATCTGGATCACCAACGA
GATCATCCACAACCCCCTGGTCAACCGCCACCTGCAGGAGATGGGGATCCGCTTTGTGGAGAAAGGCCCGGACGGCAAGA
AAGACTTTTCCCAGATCCGGCCTGAGGACGTGGTGATCTGGCCTGCCTTCGGGGCAAGTGTCTCAGAAATGGAGTTTTTC
CGGTCGCAGGGCAACGAGATCGTCGATACCACCTGCCCCTGGGTATCCCGTGTCTGGAACCAGGTGGACAAGCACCGCGC
CGCTCAATTCACCTCTGTGATCCACGGCAAGTACAACCACGAAGAAACCGTGGCCACCAGCTCCTTTGCCCAAAAATATC
TGGTGGTGCTGAACCTGGAAGAGGCGGAATGGGTCGCCCGCTACATTTTGCACGGCGGAGATGCGGCAGAATTTCGGCGC
CGCTTCGGCAAGGCCACATCTCCCGGCTTCGATCCGGATCGGGATCTGGAGCGGATCGGCATCGCCAACCAGACCACGAT
GCTGGAAGGGGAAACGCGCGCCATTGCCAAGCTGTTTGAGACCACCATGCTGCGCAAATATGGCCCCCAGGAGCTGGACA
AGCACTTCATGAGCTTTAACACCATCTGCAACGCCACCCAAGAACGGCAGGACGCCATGTTCGAGCTGCTGGAGCGGCCC
TTGGACGTGGTGGTGGTAATCGGCGGGTTTAACTCCTCCAACACCACCCACCTGCAAGAAATTGCCCTGGCCAAGGGGCT
GCCCTCCTATCACATCGATGGCCCCGACTGCATCCAGCCCCCCAACCGCATCCGCCACAAGCCCCTCCACCAAGACCCAA
TTGTTACCGAGGGGTGGCTGCCCGACGGGCCAGTGCAGATTGGTATTACCTCCGGTGCCTCTACCCCCGACCGCGTCGTG
GAGGAGGTGATCCTACGCATCTTTGCCCTGCGCGAACAGCCGCAGACCCCTCCGCAGGGATCCCTTCCCCTCGGCTTCTA
G

Upstream 100 bases:

>100_bases
TGGGCTGTCCTGCCGCCGGAGTGACCCACGCACTCAAGACAGGGCGTAAGCTGAGAGAGGATCCCTTTGCTGTTAGCGGT
GCACAGCCCGAGTGACAACC

Downstream 100 bases:

>100_bases
AAGGCGTGCTCACCACTCTTTTTCAGGTCTACCTCCCCCTGCTGGGCTGGACGCTGCTGGGGTTTGTCCTGCAGAGAGGG
CTACCTCCCCCCTGGCGCCA

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MPHSQPSSPASSPQDPRALRKALRSSSNYFAKGFEEHKDQAQRELQTTYKSPLVEQIRAQSYCYRQGDITIHLAKAFGFC
WGVERAVEYAYEARRRFPDRQIWITNEIIHNPLVNRHLQEMGIRFVEKGPDGKKDFSQIRPEDVVIWPAFGASVSEMEFF
RSQGNEIVDTTCPWVSRVWNQVDKHRAAQFTSVIHGKYNHEETVATSSFAQKYLVVLNLEEAEWVARYILHGGDAAEFRR
RFGKATSPGFDPDRDLERIGIANQTTMLEGETRAIAKLFETTMLRKYGPQELDKHFMSFNTICNATQERQDAMFELLERP
LDVVVVIGGFNSSNTTHLQEIALAKGLPSYHIDGPDCIQPPNRIRHKPLHQDPIVTEGWLPDGPVQIGITSGASTPDRVV
EEVILRIFALREQPQTPPQGSLPLGF

Sequences:

>Translated_426_residues
MPHSQPSSPASSPQDPRALRKALRSSSNYFAKGFEEHKDQAQRELQTTYKSPLVEQIRAQSYCYRQGDITIHLAKAFGFC
WGVERAVEYAYEARRRFPDRQIWITNEIIHNPLVNRHLQEMGIRFVEKGPDGKKDFSQIRPEDVVIWPAFGASVSEMEFF
RSQGNEIVDTTCPWVSRVWNQVDKHRAAQFTSVIHGKYNHEETVATSSFAQKYLVVLNLEEAEWVARYILHGGDAAEFRR
RFGKATSPGFDPDRDLERIGIANQTTMLEGETRAIAKLFETTMLRKYGPQELDKHFMSFNTICNATQERQDAMFELLERP
LDVVVVIGGFNSSNTTHLQEIALAKGLPSYHIDGPDCIQPPNRIRHKPLHQDPIVTEGWLPDGPVQIGITSGASTPDRVV
EEVILRIFALREQPQTPPQGSLPLGF
>Mature_425_residues
PHSQPSSPASSPQDPRALRKALRSSSNYFAKGFEEHKDQAQRELQTTYKSPLVEQIRAQSYCYRQGDITIHLAKAFGFCW
GVERAVEYAYEARRRFPDRQIWITNEIIHNPLVNRHLQEMGIRFVEKGPDGKKDFSQIRPEDVVIWPAFGASVSEMEFFR
SQGNEIVDTTCPWVSRVWNQVDKHRAAQFTSVIHGKYNHEETVATSSFAQKYLVVLNLEEAEWVARYILHGGDAAEFRRR
FGKATSPGFDPDRDLERIGIANQTTMLEGETRAIAKLFETTMLRKYGPQELDKHFMSFNTICNATQERQDAMFELLERPL
DVVVVIGGFNSSNTTHLQEIALAKGLPSYHIDGPDCIQPPNRIRHKPLHQDPIVTEGWLPDGPVQIGITSGASTPDRVVE
EVILRIFALREQPQTPPQGSLPLGF

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 48443; Mature: 48312

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHSQPSSPASSPQDPRALRKALRSSSNYFAKGFEEHKDQAQRELQTTYKSPLVEQIRAQ
CCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYCYRQGDITIHLAKAFGFCWGVERAVEYAYEARRRFPDRQIWITNEIIHNPLVNRHLQE
HHHEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCHHHHHHHHH
MGIRFVEKGPDGKKDFSQIRPEDVVIWPAFGASVSEMEFFRSQGNEIVDTTCPWVSRVWN
HCHHHHHCCCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHH
QVDKHRAAQFTSVIHGKYNHEETVATSSFAQKYLVVLNLEEAEWVARYILHGGDAAEFRR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECHHHHHHHHHHHCCCCHHHHHH
RFGKATSPGFDPDRDLERIGIANQTTMLEGETRAIAKLFETTMLRKYGPQELDKHFMSFN
HHCCCCCCCCCCCCHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TICNATQERQDAMFELLERPLDVVVVIGGFNSSNTTHLQEIALAKGLPSYHIDGPDCIQP
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
PNRIRHKPLHQDPIVTEGWLPDGPVQIGITSGASTPDRVVEEVILRIFALREQPQTPPQG
CHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SLPLGF
CCCCCC
>Mature Secondary Structure 
PHSQPSSPASSPQDPRALRKALRSSSNYFAKGFEEHKDQAQRELQTTYKSPLVEQIRAQ
CCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYCYRQGDITIHLAKAFGFCWGVERAVEYAYEARRRFPDRQIWITNEIIHNPLVNRHLQE
HHHEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHCHHHHHHHHH
MGIRFVEKGPDGKKDFSQIRPEDVVIWPAFGASVSEMEFFRSQGNEIVDTTCPWVSRVWN
HCHHHHHCCCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHHHCCCCEECCCCHHHHHHHH
QVDKHRAAQFTSVIHGKYNHEETVATSSFAQKYLVVLNLEEAEWVARYILHGGDAAEFRR
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECHHHHHHHHHHHCCCCHHHHHH
RFGKATSPGFDPDRDLERIGIANQTTMLEGETRAIAKLFETTMLRKYGPQELDKHFMSFN
HHCCCCCCCCCCCCHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TICNATQERQDAMFELLERPLDVVVVIGGFNSSNTTHLQEIALAKGLPSYHIDGPDCIQP
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
PNRIRHKPLHQDPIVTEGWLPDGPVQIGITSGASTPDRVVEEVILRIFALREQPQTPPQG
CHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
SLPLGF
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]