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The map label for this gene is grpE

Identifier: 86605671

GI number: 86605671

Start: 982540

End: 983253

Strand: Reverse

Name: grpE

Synonym: CYA_0971

Alternate gene names: 86605671

Gene position: 983253-982540 (Counterclockwise)

Preceding gene: 86605672

Following gene: 86605670

Centisome position: 33.53

GC content: 56.86

Gene sequence:

>714_bases
ATGCCCACAGAAGATGCCTATCCTGAACTCCAGGAAGAGGAAATCGACGTAGAAGCAGAGCTGGAGAAGCTGATTTTGGA
AGACCGTGAAGCGGAAGCCAGCACCTCCTCTGGCGAAGCCAGCGCTGAGGCTTCTCAGGATCTCAGCGAAACCTTAAAGC
AGCTCCAGCAGGAGTTGGAAATAACGCGCCAGCAACTGAAAGAGAAGGAAGAGTCCTACATCCGCCTCTATGCCGATTTT
GAGAACTACCGACGGCGTACCCAGCGGGAAAAAGAGGAGTTCAGCCAAAAAGAACGGCAGAAATTTGTGCTGGAGATCCT
GCCTGTGGTGGATAGCTTTGAGCGGGCGCAGCAGCAGTTAAAGCTGGAGACCGACCGCGAGCGGGAGGTGCACAACAGCT
ACCAAAGCGTCTATCGCTTGCTGGTGGAATGCTTGAAAAAGATGGGCGTATCCCGCATGAAATCGGTAGGCCAGCCCTTC
GATCCTAACCTGCACGAGGCCATAGCCCGCCAGCCCAGCAGCGAGTACCCCGAAGATGTGGTGGCCGTCGAATATCAACC
GGGCTACAAGCTGGGGGACTTGGTGATTCGCCACGCCATGGTGGCTGTCTCGTCGGGCAGCCCAACCTCTGAACCGTCTC
CCTCAGACCCAGCCACCCCCAAACCCGAGCCGGAAAGCACGCCTGCCTCTCCCCAAAACCCCCAACATTCGTGA

Upstream 100 bases:

>100_bases
CGACGCTGGTTTTGAGACTGCCAAACTGTCGGCACCCGACGTTACTGCTCCTTAATCACGCTTGTCGACATAACCCATGA
CTGCTGAAGAAAAGCAACCG

Downstream 100 bases:

>100_bases
CGTACTCCCGCACTCATGCTGACGCATAGAGTGCGGGCTTCCCAGTGACTCCTGCCCAATGGCAGGACATTTCCTGAGCT
TGAAGAACGGGACGCCCCAC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MPTEDAYPELQEEEIDVEAELEKLILEDREAEASTSSGEASAEASQDLSETLKQLQQELEITRQQLKEKEESYIRLYADF
ENYRRRTQREKEEFSQKERQKFVLEILPVVDSFERAQQQLKLETDREREVHNSYQSVYRLLVECLKKMGVSRMKSVGQPF
DPNLHEAIARQPSSEYPEDVVAVEYQPGYKLGDLVIRHAMVAVSSGSPTSEPSPSDPATPKPEPESTPASPQNPQHS

Sequences:

>Translated_237_residues
MPTEDAYPELQEEEIDVEAELEKLILEDREAEASTSSGEASAEASQDLSETLKQLQQELEITRQQLKEKEESYIRLYADF
ENYRRRTQREKEEFSQKERQKFVLEILPVVDSFERAQQQLKLETDREREVHNSYQSVYRLLVECLKKMGVSRMKSVGQPF
DPNLHEAIARQPSSEYPEDVVAVEYQPGYKLGDLVIRHAMVAVSSGSPTSEPSPSDPATPKPEPESTPASPQNPQHS
>Mature_236_residues
PTEDAYPELQEEEIDVEAELEKLILEDREAEASTSSGEASAEASQDLSETLKQLQQELEITRQQLKEKEESYIRLYADFE
NYRRRTQREKEEFSQKERQKFVLEILPVVDSFERAQQQLKLETDREREVHNSYQSVYRLLVECLKKMGVSRMKSVGQPFD
PNLHEAIARQPSSEYPEDVVAVEYQPGYKLGDLVIRHAMVAVSSGSPTSEPSPSDPATPKPEPESTPASPQNPQHS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=145, Percent_Identity=35.1724137931034, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1788967, Length=181, Percent_Identity=30.939226519337, Blast_Score=81, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=180, Percent_Identity=31.6666666666667, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_SYNJA (Q2JVR0)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_474434.1
- ProteinModelPortal:   Q2JVR0
- SMR:   Q2JVR0
- STRING:   Q2JVR0
- GeneID:   3898328
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_0971
- TIGR:   CYA_0971
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- ProtClustDB:   PRK14143
- BioCyc:   SSP321327:CYA_0971-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 27112; Mature: 26981

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTEDAYPELQEEEIDVEAELEKLILEDREAEASTSSGEASAEASQDLSETLKQLQQELE
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ITRQQLKEKEESYIRLYADFENYRRRTQREKEEFSQKERQKFVLEILPVVDSFERAQQQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLETDREREVHNSYQSVYRLLVECLKKMGVSRMKSVGQPFDPNLHEAIARQPSSEYPEDV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCE
VAVEYQPGYKLGDLVIRHAMVAVSSGSPTSEPSPSDPATPKPEPESTPASPQNPQHS
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTEDAYPELQEEEIDVEAELEKLILEDREAEASTSSGEASAEASQDLSETLKQLQQELE
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ITRQQLKEKEESYIRLYADFENYRRRTQREKEEFSQKERQKFVLEILPVVDSFERAQQQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLETDREREVHNSYQSVYRLLVECLKKMGVSRMKSVGQPFDPNLHEAIARQPSSEYPEDV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCCE
VAVEYQPGYKLGDLVIRHAMVAVSSGSPTSEPSPSDPATPKPEPESTPASPQNPQHS
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA