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The map label for this gene is hisH

Identifier: 86605670

GI number: 86605670

Start: 980722

End: 981372

Strand: Reverse

Name: hisH

Synonym: CYA_0969

Alternate gene names: 86605670

Gene position: 981372-980722 (Counterclockwise)

Preceding gene: 86605671

Following gene: 86605669

Centisome position: 33.46

GC content: 62.52

Gene sequence:

>651_bases
ATGAAAGCCGTGCGCCTGGCGGTGATCGACTATGATGCCGGCAACCTGCATTCAGCTTGCAAAGGGCTGGAGCGGGCCGG
GGCCGAGGTGCAGCTAGTCACAGAGCCGGCTGGGCTAGCGGCTTTTGATGGCCTTGTCCTGCCCGGCGATGGGGCCTTTG
ACCCAGCCATGCAGCAGTTGCGGGCGCGCGGCTTTGGGGAGGCAATCCGACAAGCCATCGAGCGGCAGCAGCCTTTCTTG
GGCATTTGTATTGGTTTGCAAGTTTTGTTCGAAGACAGCGAAGAGGGGACAGAGCCGGGGCTGGGCATCGTGCCGGGGCG
GGTGCAGCGCTTTCGACCGGAGCCGGGCCTGCGCATCCCCCACATGGGCTGGAACCAGTTGCACCTTACCCAGCCCGACT
CTCCCCTTTGGGCAGGGATCCCCGAGCAAAGCTGGGTTTACTTTGTCCACTCCTACCACGCGGTGCCCAAGGATCCAAGC
TGGGTGGTCGCCACCACCCAGCACGGCAGCCAAACCTGTGTGGCCGCCATTGCCCGCGGCACCTTGTTTGCCACCCAATT
TCACCCCGAAAAGTCCGGCCCTGTAGGCTTGAAGATATTGCAAAACTTTGTCGAGTTTGTTGCCCGTCGCTTGCCTGCTG
CCGGCGCCTAG

Upstream 100 bases:

>100_bases
GGTACAGAGCGGGGCTTCCAGGACTACGTCCCGCTAGCGCGAACGGCGGATTAGCTAAAACCACTATACTGATAGGCTCT
TGAAGGTTTCAGGAAAAGCC

Downstream 100 bases:

>100_bases
CGTTTCATGAACTACTGGCTGATGAAATCGGAGCCCAGCGTCTACAGCATCTGGGATCTGCAGCGGGAGGGCCAAACTCT
CTGGGACGGCGTGCGCAACT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKAVRLAVIDYDAGNLHSACKGLERAGAEVQLVTEPAGLAAFDGLVLPGDGAFDPAMQQLRARGFGEAIRQAIERQQPFL
GICIGLQVLFEDSEEGTEPGLGIVPGRVQRFRPEPGLRIPHMGWNQLHLTQPDSPLWAGIPEQSWVYFVHSYHAVPKDPS
WVVATTQHGSQTCVAAIARGTLFATQFHPEKSGPVGLKILQNFVEFVARRLPAAGA

Sequences:

>Translated_216_residues
MKAVRLAVIDYDAGNLHSACKGLERAGAEVQLVTEPAGLAAFDGLVLPGDGAFDPAMQQLRARGFGEAIRQAIERQQPFL
GICIGLQVLFEDSEEGTEPGLGIVPGRVQRFRPEPGLRIPHMGWNQLHLTQPDSPLWAGIPEQSWVYFVHSYHAVPKDPS
WVVATTQHGSQTCVAAIARGTLFATQFHPEKSGPVGLKILQNFVEFVARRLPAAGA
>Mature_216_residues
MKAVRLAVIDYDAGNLHSACKGLERAGAEVQLVTEPAGLAAFDGLVLPGDGAFDPAMQQLRARGFGEAIRQAIERQQPFL
GICIGLQVLFEDSEEGTEPGLGIVPGRVQRFRPEPGLRIPHMGWNQLHLTQPDSPLWAGIPEQSWVYFVHSYHAVPKDPS
WVVATTQHGSQTCVAAIARGTLFATQFHPEKSGPVGLKILQNFVEFVARRLPAAGA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=204, Percent_Identity=36.7647058823529, Blast_Score=125, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=36.8421052631579, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_SYNJA (Q2JVR1)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_474433.1
- ProteinModelPortal:   Q2JVR1
- SMR:   Q2JVR1
- STRING:   Q2JVR1
- GeneID:   3898364
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_0969
- TIGR:   CYA_0969
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13141
- BioCyc:   SSP321327:CYA_0969-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23395; Mature: 23395

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 83-83 ACT_SITE 188-188 ACT_SITE 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVRLAVIDYDAGNLHSACKGLERAGAEVQLVTEPAGLAAFDGLVLPGDGAFDPAMQQL
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHCCEEECCCCCCCHHHHHH
RARGFGEAIRQAIERQQPFLGICIGLQVLFEDSEEGTEPGLGIVPGRVQRFRPEPGLRIP
HHCCHHHHHHHHHHHCCCCEEHHHHHHHEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
HMGWNQLHLTQPDSPLWAGIPEQSWVYFVHSYHAVPKDPSWVVATTQHGSQTCVAAIARG
CCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHC
TLFATQFHPEKSGPVGLKILQNFVEFVARRLPAAGA
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKAVRLAVIDYDAGNLHSACKGLERAGAEVQLVTEPAGLAAFDGLVLPGDGAFDPAMQQL
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHCCEEECCCCCCCHHHHHH
RARGFGEAIRQAIERQQPFLGICIGLQVLFEDSEEGTEPGLGIVPGRVQRFRPEPGLRIP
HHCCHHHHHHHHHHHCCCCEEHHHHHHHEECCCCCCCCCCCCCCCCHHHHCCCCCCCCCC
HMGWNQLHLTQPDSPLWAGIPEQSWVYFVHSYHAVPKDPSWVVATTQHGSQTCVAAIARG
CCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHC
TLFATQFHPEKSGPVGLKILQNFVEFVARRLPAAGA
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA