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The map label for this gene is ligA

Identifier: 86605509

GI number: 86605509

Start: 814452

End: 816473

Strand: Reverse

Name: ligA

Synonym: CYA_0803

Alternate gene names: 86605509

Gene position: 816473-814452 (Counterclockwise)

Preceding gene: 86605510

Following gene: 86605508

Centisome position: 27.84

GC content: 65.33

Gene sequence:

>2022_bases
ATGGATCCAAAATTGGAACAGCGCGCCCGCGAGCTGCGCGCTCTGCTGCAGAAGGCCAGCATTGCCTACTACGTCCACGA
TGCCCCCATTCTGGAAGACAGCGTTTACGACCGCCTCTACCGCGAGCTGCAGGAGCTGGAGCGGGCCTACCCCGAGCTCA
TCACCCCCGACAGCCCCACCCAGCGGGTAGGGGAAAAGCCCGCCGAGCAGTTCCCCACCGTTTCCCACCGCATCCCCCTC
TACAGTCTGGAAAACGCCTTCAACCTGGAAGAGCTAAAGGAATGGCAAGAGCGCCTGTGGCGGGTTCTGGGGCGCACCCC
TGAGGAAGGGGAGCTGGAATATGTGTGCGAGCTGAAAATTGACGGCGCTGCCCTGGCTCTTACCTATGTGGATGGGCTGC
TGCAGCGGGGGGCCACCCGCGGCGATGGACAGTCAGGTGAGGACATTACCCCCAATATCCGCGCCATCCCTTCTATTCCC
CTGCGCTTGGCAACCGCCGCTCCCCCGCCGGTCCTGGAGGTGCGAGGAGAGGCCTATCTCCCCATTGCTGAGTTCGAGCG
CATCAACCGGGAGCGATGCTCCGCCGGGGATCCCCCCTTTGCCAACCCGCGCAACTGCGCCGCCGGCACCTTGCGCCAGT
TGGATAGCCGCGTGGTTGCCGCCCGCAAGCTCAGCTTCTTCGCCTATGCCCTCCACTGGCCGGAGGGGTGGCCAAGCGGG
GATCCCCCCCAAACCCAGTGGGAGTGTCTGCAGCGGCTAAAAGACCTGGGATTGCCCGTCAATCCTCTGTCCCAGGTGTG
CCGGGGCCTCGAGGAGGTGGTCCAGTTCTACGAGCGGTGGCGGCAGGCCCAGCTTCCCTATGCCTGTGACGGTGTGGTAG
TGAAGCTCAACTCCCTGCGGCTGCAAGAGGAGGCCGGCTTTACGCAAAAATTCCCCCGCTGGGCCATTGCCCTCAAATAC
CCTGCCCAGGAAGTCCCCACCCGCATTCGCGCCATCGTGGCCAGCGTAGGCCGCACCGGAGCCGTCACGCCGGTGGCGGA
GCTGGAGCCGGTTCTTCTGGCGGGCACCACCGTCAGCCGCGCCAGCCTGCACAATGCCGATCGCCTGGAAGAGCTGGATG
TCCACATCGGCGACACCGCCATCGTGCGCAAGGCCGGGGAGATCATCCCTGAGGTGGTGGGGATCCTCAAGGAGCTGCGC
CCCCCCGATGCCGTTCCCTACCGCCTGCCCAGCCACTGTCCCGAGTGCGGCACCCTTTTGGTTCGCCCTGCCGGCGAGGC
GATAACCCGCTGCCCCAACCCAGCTTGTCCGGCCAAGATCAGGGGGCAACTGCAGCACTGGGCCAGCCGCGACGCCATGG
ATATCGAGGGCCTGGGGGAAAAGCGGGTGGCCCAGTTAGTAGAAAAGCTGGGGGTGCGCACCGTGGCCGATCTCTACCGC
CTCACCCCGGAACACTTGGAGAGCCTGGAGGGGATGGGATCCCGTTCCAGCCAGAAGCTGGTGCAGGCCATCCAGCGCTC
CCGTCAACAGCCCTGGGAGCGCGTGCTCTACGGGCTGGGGATCCCCCATGTGGGGGTGGTGACCGCCCAGATTTTGGCCC
AGCATTTCCCCGCCCCAGAACTCTTGGCCCAGGCCAGGGCTGAAGACATCGCCCAAATCTACGGGATAGGGGCGGAAATT
GCCGAGGCGGTGGTGGCCTGGTTTGCCGATCCCGCCCACCAGAAGTTGCTGCAGGAGCTGCGGGAGCTGGGGATCCCTGC
CCTGCCCCGGCAAGCGGCCCCTGCCGTCTCCCAGGTTCTGGCTGGGAAGAAGTTTGTCATCACCGGCACCCTGCCTACTC
TCTCCCGCCAGCAGGCCAAGGCTTGGATTGAAAGCCGCGGCGGCAAAGTGACCGCCAGCGTCAGCCGCCAAACCGACTAT
GTGGTGGTAGGGTCGGATCCCGGCTCCAAACTCAAGCAGGCCCAGGCTTTGGGGATCCCTCTCCTGAGCGAGGCGGAGCT
GCTGGCGTTGGATCCGAAGTGA

Upstream 100 bases:

>100_bases
TTTTTGGGGGTTAGGCTGACAAGGGGGCAGAGCAGCGGCAGCCTGAGAGAGATGAGAGCGTTGAACCTAAGCTAGACCTA
GCCAACGGTGCAACAAGAGT

Downstream 100 bases:

>100_bases
TCTCCATCCTTGACGAGAAAAAATTTGGGAAGGCCGGCTTGTCCTCGCCCTGGCGATGGTTTATCATGCGATTCTGTGAG
GAGTGGACGCCAGAGGGGGA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 673; Mature: 673

Protein sequence:

>673_residues
MDPKLEQRARELRALLQKASIAYYVHDAPILEDSVYDRLYRELQELERAYPELITPDSPTQRVGEKPAEQFPTVSHRIPL
YSLENAFNLEELKEWQERLWRVLGRTPEEGELEYVCELKIDGAALALTYVDGLLQRGATRGDGQSGEDITPNIRAIPSIP
LRLATAAPPPVLEVRGEAYLPIAEFERINRERCSAGDPPFANPRNCAAGTLRQLDSRVVAARKLSFFAYALHWPEGWPSG
DPPQTQWECLQRLKDLGLPVNPLSQVCRGLEEVVQFYERWRQAQLPYACDGVVVKLNSLRLQEEAGFTQKFPRWAIALKY
PAQEVPTRIRAIVASVGRTGAVTPVAELEPVLLAGTTVSRASLHNADRLEELDVHIGDTAIVRKAGEIIPEVVGILKELR
PPDAVPYRLPSHCPECGTLLVRPAGEAITRCPNPACPAKIRGQLQHWASRDAMDIEGLGEKRVAQLVEKLGVRTVADLYR
LTPEHLESLEGMGSRSSQKLVQAIQRSRQQPWERVLYGLGIPHVGVVTAQILAQHFPAPELLAQARAEDIAQIYGIGAEI
AEAVVAWFADPAHQKLLQELRELGIPALPRQAAPAVSQVLAGKKFVITGTLPTLSRQQAKAWIESRGGKVTASVSRQTDY
VVVGSDPGSKLKQAQALGIPLLSEAELLALDPK

Sequences:

>Translated_673_residues
MDPKLEQRARELRALLQKASIAYYVHDAPILEDSVYDRLYRELQELERAYPELITPDSPTQRVGEKPAEQFPTVSHRIPL
YSLENAFNLEELKEWQERLWRVLGRTPEEGELEYVCELKIDGAALALTYVDGLLQRGATRGDGQSGEDITPNIRAIPSIP
LRLATAAPPPVLEVRGEAYLPIAEFERINRERCSAGDPPFANPRNCAAGTLRQLDSRVVAARKLSFFAYALHWPEGWPSG
DPPQTQWECLQRLKDLGLPVNPLSQVCRGLEEVVQFYERWRQAQLPYACDGVVVKLNSLRLQEEAGFTQKFPRWAIALKY
PAQEVPTRIRAIVASVGRTGAVTPVAELEPVLLAGTTVSRASLHNADRLEELDVHIGDTAIVRKAGEIIPEVVGILKELR
PPDAVPYRLPSHCPECGTLLVRPAGEAITRCPNPACPAKIRGQLQHWASRDAMDIEGLGEKRVAQLVEKLGVRTVADLYR
LTPEHLESLEGMGSRSSQKLVQAIQRSRQQPWERVLYGLGIPHVGVVTAQILAQHFPAPELLAQARAEDIAQIYGIGAEI
AEAVVAWFADPAHQKLLQELRELGIPALPRQAAPAVSQVLAGKKFVITGTLPTLSRQQAKAWIESRGGKVTASVSRQTDY
VVVGSDPGSKLKQAQALGIPLLSEAELLALDPK
>Mature_673_residues
MDPKLEQRARELRALLQKASIAYYVHDAPILEDSVYDRLYRELQELERAYPELITPDSPTQRVGEKPAEQFPTVSHRIPL
YSLENAFNLEELKEWQERLWRVLGRTPEEGELEYVCELKIDGAALALTYVDGLLQRGATRGDGQSGEDITPNIRAIPSIP
LRLATAAPPPVLEVRGEAYLPIAEFERINRERCSAGDPPFANPRNCAAGTLRQLDSRVVAARKLSFFAYALHWPEGWPSG
DPPQTQWECLQRLKDLGLPVNPLSQVCRGLEEVVQFYERWRQAQLPYACDGVVVKLNSLRLQEEAGFTQKFPRWAIALKY
PAQEVPTRIRAIVASVGRTGAVTPVAELEPVLLAGTTVSRASLHNADRLEELDVHIGDTAIVRKAGEIIPEVVGILKELR
PPDAVPYRLPSHCPECGTLLVRPAGEAITRCPNPACPAKIRGQLQHWASRDAMDIEGLGEKRVAQLVEKLGVRTVADLYR
LTPEHLESLEGMGSRSSQKLVQAIQRSRQQPWERVLYGLGIPHVGVVTAQILAQHFPAPELLAQARAEDIAQIYGIGAEI
AEAVVAWFADPAHQKLLQELRELGIPALPRQAAPAVSQVLAGKKFVITGTLPTLSRQQAKAWIESRGGKVTASVSRQTDY
VVVGSDPGSKLKQAQALGIPLLSEAELLALDPK

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=671, Percent_Identity=45.0074515648286, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI87082305, Length=567, Percent_Identity=23.9858906525573, Blast_Score=129, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74348; Mature: 74348

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPKLEQRARELRALLQKASIAYYVHDAPILEDSVYDRLYRELQELERAYPELITPDSPT
CCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
QRVGEKPAEQFPTVSHRIPLYSLENAFNLEELKEWQERLWRVLGRTPEEGELEYVCELKI
HHHCCCCHHHCCCCHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEE
DGAALALTYVDGLLQRGATRGDGQSGEDITPNIRAIPSIPLRLATAAPPPVLEVRGEAYL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCC
PIAEFERINRERCSAGDPPFANPRNCAAGTLRQLDSRVVAARKLSFFAYALHWPEGWPSG
CHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
DPPQTQWECLQRLKDLGLPVNPLSQVCRGLEEVVQFYERWRQAQLPYACDGVVVKLNSLR
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHH
LQEEAGFTQKFPRWAIALKYPAQEVPTRIRAIVASVGRTGAVTPVAELEPVLLAGTTVSR
HHHHCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEECCCHHH
ASLHNADRLEELDVHIGDTAIVRKAGEIIPEVVGILKELRPPDAVPYRLPSHCPECGTLL
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEE
VRPAGEAITRCPNPACPAKIRGQLQHWASRDAMDIEGLGEKRVAQLVEKLGVRTVADLYR
ECCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
LTPEHLESLEGMGSRSSQKLVQAIQRSRQQPWERVLYGLGIPHVGVVTAQILAQHFPAPE
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
LLAQARAEDIAQIYGIGAEIAEAVVAWFADPAHQKLLQELRELGIPALPRQAAPAVSQVL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AGKKFVITGTLPTLSRQQAKAWIESRGGKVTASVSRQTDYVVVGSDPGSKLKQAQALGIP
CCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHCCC
LLSEAELLALDPK
CCCCCCEEEECCC
>Mature Secondary Structure
MDPKLEQRARELRALLQKASIAYYVHDAPILEDSVYDRLYRELQELERAYPELITPDSPT
CCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
QRVGEKPAEQFPTVSHRIPLYSLENAFNLEELKEWQERLWRVLGRTPEEGELEYVCELKI
HHHCCCCHHHCCCCHHCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEE
DGAALALTYVDGLLQRGATRGDGQSGEDITPNIRAIPSIPLRLATAAPPPVLEVRGEAYL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEECCCCCC
PIAEFERINRERCSAGDPPFANPRNCAAGTLRQLDSRVVAARKLSFFAYALHWPEGWPSG
CHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
DPPQTQWECLQRLKDLGLPVNPLSQVCRGLEEVVQFYERWRQAQLPYACDGVVVKLNSLR
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHH
LQEEAGFTQKFPRWAIALKYPAQEVPTRIRAIVASVGRTGAVTPVAELEPVLLAGTTVSR
HHHHCCCHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEEECCCHHH
ASLHNADRLEELDVHIGDTAIVRKAGEIIPEVVGILKELRPPDAVPYRLPSHCPECGTLL
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEE
VRPAGEAITRCPNPACPAKIRGQLQHWASRDAMDIEGLGEKRVAQLVEKLGVRTVADLYR
ECCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHH
LTPEHLESLEGMGSRSSQKLVQAIQRSRQQPWERVLYGLGIPHVGVVTAQILAQHFPAPE
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHH
LLAQARAEDIAQIYGIGAEIAEAVVAWFADPAHQKLLQELRELGIPALPRQAAPAVSQVL
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AGKKFVITGTLPTLSRQQAKAWIESRGGKVTASVSRQTDYVVVGSDPGSKLKQAQALGIP
CCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHCCC
LLSEAELLALDPK
CCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA