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The map label for this gene is topA

Identifier: 86605277

GI number: 86605277

Start: 572482

End: 575142

Strand: Reverse

Name: topA

Synonym: CYA_0561

Alternate gene names: 86605277

Gene position: 575142-572482 (Counterclockwise)

Preceding gene: 86605279

Following gene: 86605273

Centisome position: 19.61

GC content: 62.72

Gene sequence:

>2661_bases
ATGCCCAAGCTGGTCATTGTCGAATCTCCCACCAAAGCCCGTACCATCCGCGGCTTCTTGCCCGCCGGCTATGAGGTGCA
AGCCTCGATGGGACATGTACGCGATCTGCCGGAGTCGGCCTCTGACATTCCCGAAGAGGTGAAGGGAGAAGAGTGGAGCC
GTCTCGGGGTCAATGTCCACGCCAACTTCGAGCCTCTCTACGTGATCCCCAAGGACAAAAGAAAGGTAGTCAAACAGCTG
AAGGAGGCGCTTAAGAAAGCCGACGAGCTAATTCTGGCCACTGACGAAGACCGGGAGGGGGAAAGTATCAGTTGGCACCT
GCAACAGGTGCTGCAGCCCAAGGTGCCCACCCGCCGCATGGTGTTCCACGAGATCACCCGCGAGGCCATTCAGGAGGCGC
TGCAAAATTGCCGTGAGGTGGACGAGCAACTGGTGCGGGCCCAAGAGACGCGCCGCATCTTGGATCGCCTGGTGGGTTAC
ACTCTGTCGCCGCTGCTGTGGAAAAAAATTGCCCCCAAACTCTCGGCAGGTCGGGTGCAGTCGGTGGCGGTGAAGCTCTT
GGTGGATCGGGAGCGGGAGCGTCGCGCCTTTAAAAAAGCCACCTACTGGGATCTGAAAGCGGATTTGCAACCGAGGACAG
CAAGCCAAGCGGCCTCTTTCCCGGCAGAGCTGGTGAGCCTGGGCGGCAAACGTCTGGCCAGCGGGCAAGACTTCGATCCC
AGCACCGGGCAGCTCATAGCCGGACGGGATGTGGTGCTGTTGGATCAAGCCCAGGCCGAGGCCCTACGGGATCGCCTGCT
GGGGAAAGCTTGGGTCGTCAGCTCGGTCGAAGAACGCCCCTCCACCCGCAAGCCCGCCCCTCCCTTCACCACTTCCACGC
TGCAACAGGAGGCCAACCGCAAGCTGCGCCTGTCGGCTCAGCAGACGATGCGCATCGCCCAAAAGCTCTACGAAGAGGGC
TACATCACCTACATGCGCACCGACTCGGTACACCTGTCGGAACAGGCCATCCGCGCCGCCCGCGCCTGCGTGGAGTCGAT
GTACGGCAAGGAGTACCTCAGCCCCGAACCTCGGCAGTACACCACCAAAACCAAAGGGGCGCAAGAAGCCCACGAGGCCA
TTCGTCCCGCCGGCTCGGAGTTTCGCCTGCCCAGCCAAACCCCCCTCACAGGGCAGGAGCTGGCCCTCTACGACCTGATC
TGGAAGCGCACTGTCGCCAGCCAAATGGCCGAGGCGCGGCAGACCCACACCATCGTGCTCATCGCGGTGGACGATGCGGT
GTTTCGGGCCAGCGGCAAGCGCATTGACTTTCCCGGCTTCTTCCGCGCCTATGTGGAGGGATCCGACGACCCAGAGGCGG
CTTTGGAAGATCGGGAAGTGCATCTGCCCCTGTTGCGGCAGGGGGATCCCTTGAGCTGCCTGCAGTTGGAGCCGGTGGGC
CACGAAACCCAGCCGCCGCCTCGCTATACGGAAGCATCCTTGGTCAAGGTGTTGGAAAGCGAGGGCATTGGCCGCCCCAG
CACCTACGCCACCATCCTGAACACCATCATGAGCCGCGGCTACGTCAGGTCGATGGGGAACGCCCTCGTGCCCACCTACA
CCGCCTTTGCGGTGACCGAGCTGCTGGAAAAATACTTTCCCAACCTGGTGAACACCCGCTTCACTGCCGAGATGGAGCAA
ACCCTCGATGACATCGCTTCTGGCCAGGCAGACTGGTTGCCCTACTTGCGCCATTTCTTCCTGGGCGAGCAGGGCCTAGA
AGGCCAGGTGAAGGCGCGCGAGTTGGAAATCGACTCCGGCCTGGCCCGCTCCATTTACCTGGAGAACCTGCCCGTCAAGA
TCGGCATCGGCCCCTACGGCCCCTATGTGTCGGTAGAGACTGAATCAGGAGAGGTGCGCGCCAACCTGCCGGAGGATATT
CCCCCCGCCGATCTCAGCGCCGAACACATCGAGGAGCTCATCCGGCGCAAGCAGGAAGGTCCCAAGCCCATCGCCTTCCA
CCCGGAAACCCAAGAGCCGGTCTACGTAATTCCCCAGGGTCCCTACGGCCCCTATGTGCAGTTGGGAGAGGTGTCGGAGG
AGAACAAAAAGCCCAAGCGCACCTCCCTGCCCAGAGGGCTGAAGCCGGAGCAGGTCACCCCTGAGATTGCCCTGGGGCTG
CTCAGCCTGCCCCGCACGTTGGGGGAGCACCCGGAATCGGGGAAAAAGGTGCAGGCCGGCATTGGCCGCTTCGGCCCCTA
CGTGGTCTGCGACGGGGATTTCCGCTCGCTGCCTGCCGATGAGGATGTGCTGACCATCAGCCTAGAGCGGGCGCTAGAGC
TGCTGGCCCAGCCCAAAAAAGGCCGTGGGCGCGGCTCGGCCAAGCCCCTGCGCGAGCTGGGATCCCATCCTGAGGATGGC
GAGCCGGTCACCTTGCACGACGGCCCCTATGGTCTGTATGTCAAGCACGGCAAGGTAAACGCCTCACTGCCCAAAGATCT
CTCCCCGGAAGCCGTGACGCTGGAGATGGCGCTGGAGTTGCTGGCGGAAAAAGAAGGCAAGGGCCAGGCAAAAGCCAGCC
GACCAGCGGCGGCCAAGCGGGCCAAGAAGGCTGAAGCTGCTCCCCAGAAGCAACAATCCCATCCCACCCTGCTGGAGGCT
GTCTCCAAAGCTCGGGCTTGA

Upstream 100 bases:

>100_bases
GATAGATCCATAGGTAGACCCGATTGTGGGCGCCGATCCGCCGCACCCCTGGGTCGGGAGGCTCACCACCTTCAGTCGAT
TCCTTCTAGTCAACATCATC

Downstream 100 bases:

>100_bases
GCGGTGGGGGTAGGGGCAACTTGCTGGCTAACTCGGCTTGTTCTGCAAGGAAACTAAGCAGCTTGTTCAAAAATGCTCCG
AACCAAGCTGCTAATGATGG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 886; Mature: 885

Protein sequence:

>886_residues
MPKLVIVESPTKARTIRGFLPAGYEVQASMGHVRDLPESASDIPEEVKGEEWSRLGVNVHANFEPLYVIPKDKRKVVKQL
KEALKKADELILATDEDREGESISWHLQQVLQPKVPTRRMVFHEITREAIQEALQNCREVDEQLVRAQETRRILDRLVGY
TLSPLLWKKIAPKLSAGRVQSVAVKLLVDRERERRAFKKATYWDLKADLQPRTASQAASFPAELVSLGGKRLASGQDFDP
STGQLIAGRDVVLLDQAQAEALRDRLLGKAWVVSSVEERPSTRKPAPPFTTSTLQQEANRKLRLSAQQTMRIAQKLYEEG
YITYMRTDSVHLSEQAIRAARACVESMYGKEYLSPEPRQYTTKTKGAQEAHEAIRPAGSEFRLPSQTPLTGQELALYDLI
WKRTVASQMAEARQTHTIVLIAVDDAVFRASGKRIDFPGFFRAYVEGSDDPEAALEDREVHLPLLRQGDPLSCLQLEPVG
HETQPPPRYTEASLVKVLESEGIGRPSTYATILNTIMSRGYVRSMGNALVPTYTAFAVTELLEKYFPNLVNTRFTAEMEQ
TLDDIASGQADWLPYLRHFFLGEQGLEGQVKARELEIDSGLARSIYLENLPVKIGIGPYGPYVSVETESGEVRANLPEDI
PPADLSAEHIEELIRRKQEGPKPIAFHPETQEPVYVIPQGPYGPYVQLGEVSEENKKPKRTSLPRGLKPEQVTPEIALGL
LSLPRTLGEHPESGKKVQAGIGRFGPYVVCDGDFRSLPADEDVLTISLERALELLAQPKKGRGRGSAKPLRELGSHPEDG
EPVTLHDGPYGLYVKHGKVNASLPKDLSPEAVTLEMALELLAEKEGKGQAKASRPAAAKRAKKAEAAPQKQQSHPTLLEA
VSKARA

Sequences:

>Translated_886_residues
MPKLVIVESPTKARTIRGFLPAGYEVQASMGHVRDLPESASDIPEEVKGEEWSRLGVNVHANFEPLYVIPKDKRKVVKQL
KEALKKADELILATDEDREGESISWHLQQVLQPKVPTRRMVFHEITREAIQEALQNCREVDEQLVRAQETRRILDRLVGY
TLSPLLWKKIAPKLSAGRVQSVAVKLLVDRERERRAFKKATYWDLKADLQPRTASQAASFPAELVSLGGKRLASGQDFDP
STGQLIAGRDVVLLDQAQAEALRDRLLGKAWVVSSVEERPSTRKPAPPFTTSTLQQEANRKLRLSAQQTMRIAQKLYEEG
YITYMRTDSVHLSEQAIRAARACVESMYGKEYLSPEPRQYTTKTKGAQEAHEAIRPAGSEFRLPSQTPLTGQELALYDLI
WKRTVASQMAEARQTHTIVLIAVDDAVFRASGKRIDFPGFFRAYVEGSDDPEAALEDREVHLPLLRQGDPLSCLQLEPVG
HETQPPPRYTEASLVKVLESEGIGRPSTYATILNTIMSRGYVRSMGNALVPTYTAFAVTELLEKYFPNLVNTRFTAEMEQ
TLDDIASGQADWLPYLRHFFLGEQGLEGQVKARELEIDSGLARSIYLENLPVKIGIGPYGPYVSVETESGEVRANLPEDI
PPADLSAEHIEELIRRKQEGPKPIAFHPETQEPVYVIPQGPYGPYVQLGEVSEENKKPKRTSLPRGLKPEQVTPEIALGL
LSLPRTLGEHPESGKKVQAGIGRFGPYVVCDGDFRSLPADEDVLTISLERALELLAQPKKGRGRGSAKPLRELGSHPEDG
EPVTLHDGPYGLYVKHGKVNASLPKDLSPEAVTLEMALELLAEKEGKGQAKASRPAAAKRAKKAEAAPQKQQSHPTLLEA
VSKARA
>Mature_885_residues
PKLVIVESPTKARTIRGFLPAGYEVQASMGHVRDLPESASDIPEEVKGEEWSRLGVNVHANFEPLYVIPKDKRKVVKQLK
EALKKADELILATDEDREGESISWHLQQVLQPKVPTRRMVFHEITREAIQEALQNCREVDEQLVRAQETRRILDRLVGYT
LSPLLWKKIAPKLSAGRVQSVAVKLLVDRERERRAFKKATYWDLKADLQPRTASQAASFPAELVSLGGKRLASGQDFDPS
TGQLIAGRDVVLLDQAQAEALRDRLLGKAWVVSSVEERPSTRKPAPPFTTSTLQQEANRKLRLSAQQTMRIAQKLYEEGY
ITYMRTDSVHLSEQAIRAARACVESMYGKEYLSPEPRQYTTKTKGAQEAHEAIRPAGSEFRLPSQTPLTGQELALYDLIW
KRTVASQMAEARQTHTIVLIAVDDAVFRASGKRIDFPGFFRAYVEGSDDPEAALEDREVHLPLLRQGDPLSCLQLEPVGH
ETQPPPRYTEASLVKVLESEGIGRPSTYATILNTIMSRGYVRSMGNALVPTYTAFAVTELLEKYFPNLVNTRFTAEMEQT
LDDIASGQADWLPYLRHFFLGEQGLEGQVKARELEIDSGLARSIYLENLPVKIGIGPYGPYVSVETESGEVRANLPEDIP
PADLSAEHIEELIRRKQEGPKPIAFHPETQEPVYVIPQGPYGPYVQLGEVSEENKKPKRTSLPRGLKPEQVTPEIALGLL
SLPRTLGEHPESGKKVQAGIGRFGPYVVCDGDFRSLPADEDVLTISLERALELLAQPKKGRGRGSAKPLRELGSHPEDGE
PVTLHDGPYGLYVKHGKVNASLPKDLSPEAVTLEMALELLAEKEGKGQAKASRPAAAKRAKKAEAAPQKQQSHPTLLEAV
SKARA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=563, Percent_Identity=25.7548845470693, Blast_Score=132, Evalue=2e-30,
Organism=Homo sapiens, GI4507635, Length=525, Percent_Identity=23.8095238095238, Blast_Score=110, Evalue=5e-24,
Organism=Escherichia coli, GI1787529, Length=594, Percent_Identity=41.4141414141414, Blast_Score=417, Evalue=1e-117,
Organism=Escherichia coli, GI1788061, Length=638, Percent_Identity=24.7648902821317, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=588, Percent_Identity=25.6802721088435, Blast_Score=126, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=529, Percent_Identity=24.3856332703214, Blast_Score=122, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6323263, Length=569, Percent_Identity=25.3075571177504, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24585251, Length=526, Percent_Identity=27.3764258555133, Blast_Score=130, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640096, Length=537, Percent_Identity=21.6014897579143, Blast_Score=98, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 98385; Mature: 98254

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00018 EF_HAND_1 ; PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLVIVESPTKARTIRGFLPAGYEVQASMGHVRDLPESASDIPEEVKGEEWSRLGVNVH
CCCEEEEECCCHHHHHHHCCCCCCEEHHHHHHHHCCCCHHHCCCHHHCCCCHHHCCEEEE
ANFEPLYVIPKDKRKVVKQLKEALKKADELILATDEDREGESISWHLQQVLQPKVPTRRM
CCCCEEEEECCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHH
VFHEITREAIQEALQNCREVDEQLVRAQETRRILDRLVGYTLSPLLWKKIAPKLSAGRVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
SVAVKLLVDRERERRAFKKATYWDLKADLQPRTASQAASFPAELVSLGGKRLASGQDFDP
HHHHHHHHHCHHHHHHHHHHHHEEEECCCCCCCHHHHHCCHHHHHHCCCHHCCCCCCCCC
STGQLIAGRDVVLLDQAQAEALRDRLLGKAWVVSSVEERPSTRKPAPPFTTSTLQQEANR
CCCCEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCC
KLRLSAQQTMRIAQKLYEEGYITYMRTDSVHLSEQAIRAARACVESMYGKEYLSPEPRQY
EEEHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCC
TTKTKGAQEAHEAIRPAGSEFRLPSQTPLTGQELALYDLIWKRTVASQMAEARQTHTIVL
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IAVDDAVFRASGKRIDFPGFFRAYVEGSDDPEAALEDREVHLPLLRQGDPLSCLQLEPVG
EEECCHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHCCCCCCCCHHCCCCCCCEEEECCCC
HETQPPPRYTEASLVKVLESEGIGRPSTYATILNTIMSRGYVRSMGNALVPTYTAFAVTE
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHH
LLEKYFPNLVNTRFTAEMEQTLDDIASGQADWLPYLRHFFLGEQGLEGQVKARELEIDSG
HHHHHCHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
LARSIYLENLPVKIGIGPYGPYVSVETESGEVRANLPEDIPPADLSAEHIEELIRRKQEG
CHHHHEEECCCEEEECCCCCCEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCC
PKPIAFHPETQEPVYVIPQGPYGPYVQLGEVSEENKKPKRTSLPRGLKPEQVTPEIALGL
CCCEEECCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHH
LSLPRTLGEHPESGKKVQAGIGRFGPYVVCDGDFRSLPADEDVLTISLERALELLAQPKK
HHHHHHHCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHCCCCC
GRGRGSAKPLRELGSHPEDGEPVTLHDGPYGLYVKHGKVNASLPKDLSPEAVTLEMALEL
CCCCCCCHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
LAEKEGKGQAKASRPAAAKRAKKAEAAPQKQQSHPTLLEAVSKARA
HHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCHHHHHHHHHCC
>Mature Secondary Structure 
PKLVIVESPTKARTIRGFLPAGYEVQASMGHVRDLPESASDIPEEVKGEEWSRLGVNVH
CCEEEEECCCHHHHHHHCCCCCCEEHHHHHHHHCCCCHHHCCCHHHCCCCHHHCCEEEE
ANFEPLYVIPKDKRKVVKQLKEALKKADELILATDEDREGESISWHLQQVLQPKVPTRRM
CCCCEEEEECCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCCCCHHHH
VFHEITREAIQEALQNCREVDEQLVRAQETRRILDRLVGYTLSPLLWKKIAPKLSAGRVQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
SVAVKLLVDRERERRAFKKATYWDLKADLQPRTASQAASFPAELVSLGGKRLASGQDFDP
HHHHHHHHHCHHHHHHHHHHHHEEEECCCCCCCHHHHHCCHHHHHHCCCHHCCCCCCCCC
STGQLIAGRDVVLLDQAQAEALRDRLLGKAWVVSSVEERPSTRKPAPPFTTSTLQQEANR
CCCCEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCC
KLRLSAQQTMRIAQKLYEEGYITYMRTDSVHLSEQAIRAARACVESMYGKEYLSPEPRQY
EEEHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCCC
TTKTKGAQEAHEAIRPAGSEFRLPSQTPLTGQELALYDLIWKRTVASQMAEARQTHTIVL
CCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IAVDDAVFRASGKRIDFPGFFRAYVEGSDDPEAALEDREVHLPLLRQGDPLSCLQLEPVG
EEECCHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHCCCCCCCCHHCCCCCCCEEEECCCC
HETQPPPRYTEASLVKVLESEGIGRPSTYATILNTIMSRGYVRSMGNALVPTYTAFAVTE
CCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHCCCCCHHHHHHHHHH
LLEKYFPNLVNTRFTAEMEQTLDDIASGQADWLPYLRHFFLGEQGLEGQVKARELEIDSG
HHHHHCHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
LARSIYLENLPVKIGIGPYGPYVSVETESGEVRANLPEDIPPADLSAEHIEELIRRKQEG
CHHHHEEECCCEEEECCCCCCEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHCC
PKPIAFHPETQEPVYVIPQGPYGPYVQLGEVSEENKKPKRTSLPRGLKPEQVTPEIALGL
CCCEEECCCCCCCEEEEECCCCCCCEEECCCCCCCCCCCHHCCCCCCCCCCCCHHHHHHH
LSLPRTLGEHPESGKKVQAGIGRFGPYVVCDGDFRSLPADEDVLTISLERALELLAQPKK
HHHHHHHCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHCCCCC
GRGRGSAKPLRELGSHPEDGEPVTLHDGPYGLYVKHGKVNASLPKDLSPEAVTLEMALEL
CCCCCCCHHHHHHCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
LAEKEGKGQAKASRPAAAKRAKKAEAAPQKQQSHPTLLEAVSKARA
HHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7659748 [H]