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The map label for this gene is dosC [H]

Identifier: 86605201

GI number: 86605201

Start: 500693

End: 501478

Strand: Reverse

Name: dosC [H]

Synonym: CYA_0483

Alternate gene names: 86605201

Gene position: 501478-500693 (Counterclockwise)

Preceding gene: 86605202

Following gene: 86605198

Centisome position: 17.1

GC content: 60.31

Gene sequence:

>786_bases
ATGGGCATGACCTTTCCCTTGGGGGAAACAGCGGTAGTGGGGCGCAGCTCCCAGGCTGACATTCAGCTCTGGGATCACGC
CGTTTCTCGGCAGCACTGTCGGCTGGAGAGAGAAGGGGGAACCTACATCCTCACCGACCTAGGCAGCCTCAACGGTACCT
ATGTCAACTGGCAGAGGGTAACTCGCCTTCCCTTGGCCGATAGGGATCGCATCCAGCTGGGGAATAGCCTCCTGCAGTTT
GCCTATTATGACCGGCTGGAGGAGGCTTTCTTTTTCCAGGTCGCTGCCGCCGCTCTCTACGACCCGCTCACGGGGCTGCA
CAACCGTCGGTTTTTCCTAGAGCACCTGCAGAGGGAGATGGCCTTTGCCCGCCGCCATAGCTTTCCCCTCCACTTGCTTT
ACCTGGACTTGGACAACTTCAAGCTCATCAACGACCGCTGGGGGCACTTGGCCGGGGATCAAGCCCTGATCCAGGTGGCC
CGCCAACTGCAGGACCAGGTTCGCCAAGAGGATCTGCTGGCCCGCATGGGGGGCGACGAATTTGTCCTCATGGTGCGCGG
GGTTCCGGAAGCTCAAATCCTGCAGTTGGCCAAGCGCCTGCGGCAGGCCGTCCAATCCCTCCCTCTGCAGGTGGGAGCGG
AAGTTATCCACCTCACCTGTAGCATTGGCGTCGCCTCTTGGCTCAGCCTTTCAGCAGAAACTTCCGTCCAAAGCTTTCTA
GAGGCAGCCGACCAAGCCCTCTACCAGGCCAAAAGGCAGGGGGGCAACCGGGAAGTGTTGCTGTAG

Upstream 100 bases:

>100_bases
TCTCGAAGAGCCGACCACCTGCATCTCCCTGGAGTGCTATGGGCTGGGAGTGGAGAGAGAGCCTGTGGGCGCCTTTTTGA
CCGTGCTCTGGGGGGCCAAT

Downstream 100 bases:

>100_bases
GGCTTAGGATCCCTCCACCAAGCTGGCGTAAGCCTCCGCGCTCATCGTGTCGTCGAGATCTTCCGGGTTGGTCATGCGCA
CCTTGATCAGCCAGCCGTCT

Product: GGDEF/FHA domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MGMTFPLGETAVVGRSSQADIQLWDHAVSRQHCRLEREGGTYILTDLGSLNGTYVNWQRVTRLPLADRDRIQLGNSLLQF
AYYDRLEEAFFFQVAAAALYDPLTGLHNRRFFLEHLQREMAFARRHSFPLHLLYLDLDNFKLINDRWGHLAGDQALIQVA
RQLQDQVRQEDLLARMGGDEFVLMVRGVPEAQILQLAKRLRQAVQSLPLQVGAEVIHLTCSIGVASWLSLSAETSVQSFL
EAADQALYQAKRQGGNREVLL

Sequences:

>Translated_261_residues
MGMTFPLGETAVVGRSSQADIQLWDHAVSRQHCRLEREGGTYILTDLGSLNGTYVNWQRVTRLPLADRDRIQLGNSLLQF
AYYDRLEEAFFFQVAAAALYDPLTGLHNRRFFLEHLQREMAFARRHSFPLHLLYLDLDNFKLINDRWGHLAGDQALIQVA
RQLQDQVRQEDLLARMGGDEFVLMVRGVPEAQILQLAKRLRQAVQSLPLQVGAEVIHLTCSIGVASWLSLSAETSVQSFL
EAADQALYQAKRQGGNREVLL
>Mature_260_residues
GMTFPLGETAVVGRSSQADIQLWDHAVSRQHCRLEREGGTYILTDLGSLNGTYVNWQRVTRLPLADRDRIQLGNSLLQFA
YYDRLEEAFFFQVAAAALYDPLTGLHNRRFFLEHLQREMAFARRHSFPLHLLYLDLDNFKLINDRWGHLAGDQALIQVAR
QLQDQVRQEDLLARMGGDEFVLMVRGVPEAQILQLAKRLRQAVQSLPLQVGAEVIHLTCSIGVASWLSLSAETSVQSFLE
AADQALYQAKRQGGNREVLL

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=263, Percent_Identity=34.2205323193916, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786584, Length=158, Percent_Identity=39.2405063291139, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=32.5, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1787802, Length=169, Percent_Identity=36.6863905325444, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87081881, Length=177, Percent_Identity=37.8531073446328, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87081977, Length=159, Percent_Identity=35.8490566037736, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1788956, Length=213, Percent_Identity=36.150234741784, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=33.5365853658537, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788085, Length=185, Percent_Identity=28.1081081081081, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787541, Length=154, Percent_Identity=33.1168831168831, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787816, Length=194, Percent_Identity=29.3814432989691, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787056, Length=165, Percent_Identity=32.7272727272727, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081974, Length=175, Percent_Identity=25.7142857142857, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788381, Length=236, Percent_Identity=29.2372881355932, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 29617; Mature: 29486

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50006 FHA_DOMAIN ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMTFPLGETAVVGRSSQADIQLWDHAVSRQHCRLEREGGTYILTDLGSLNGTYVNWQRV
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCEEEHHHH
TRLPLADRDRIQLGNSLLQFAYYDRLEEAFFFQVAAAALYDPLTGLHNRRFFLEHLQREM
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AFARRHSFPLHLLYLDLDNFKLINDRWGHLAGDQALIQVARQLQDQVRQEDLLARMGGDE
HHHHHCCCCEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
FVLMVRGVPEAQILQLAKRLRQAVQSLPLQVGAEVIHLTCSIGVASWLSLSAETSVQSFL
EEEEEECCCHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAADQALYQAKRQGGNREVLL
HHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GMTFPLGETAVVGRSSQADIQLWDHAVSRQHCRLEREGGTYILTDLGSLNGTYVNWQRV
CCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCEEEHHHH
TRLPLADRDRIQLGNSLLQFAYYDRLEEAFFFQVAAAALYDPLTGLHNRRFFLEHLQREM
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AFARRHSFPLHLLYLDLDNFKLINDRWGHLAGDQALIQVARQLQDQVRQEDLLARMGGDE
HHHHHCCCCEEEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
FVLMVRGVPEAQILQLAKRLRQAVQSLPLQVGAEVIHLTCSIGVASWLSLSAETSVQSFL
EEEEEECCCHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAADQALYQAKRQGGNREVLL
HHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]