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The map label for this gene is pflB

Identifier: 86605195

GI number: 86605195

Start: 493749

End: 496055

Strand: Reverse

Name: pflB

Synonym: CYA_0477

Alternate gene names: 86605195

Gene position: 496055-493749 (Counterclockwise)

Preceding gene: 86605197

Following gene: 86605194

Centisome position: 16.91

GC content: 61.21

Gene sequence:

>2307_bases
ATGATAGCAAGCGCGCAGCGTTCTGTAGCTTTATCTACAGAATCGAAGGCCCAAAAAACCACAGAACGCAAAGTATCCGA
TCAAACGATTGGGAGAGATTTTCTGCCGGGACGGTGGCAACGCTGCATTGATGTCCGAGATTTTATTCAGCGCAATTACA
CTCCCTACACCGGCGACGAAAGCTTCTTGGCGCCGGCCAGCGAACGCACGCAGCGGCTGTGGGCCAAGGTTAAGGATTTG
ATGGCGCTGGAGCGGGAGCGGGGGGTTTTGGATGCCGACACGGACCTTCCTTCCAGCATCACCAGCCACCCGCCGGGCTA
TATCGACCGGGAGCTGGAGCAAATTGTAGGCCTACAGACCGACAAGCCCCTGAAGCGGGCCATTATGCCGTTCGGCGGCA
TCCGCATGGTGGAGACGGCCCTCAAGGCCTACGGCTACGAGCTGGATCCAAAGGTTAAGGAAATCTTTACCAAGTACCGC
AAGACCCACAACGACGGGGTTTTCGACGCCTACACGGAGGAGATGCGCCGCTGCCGCCGCTCTGGCATCATCACCGGCTT
GCCGGACGCCTATGGACGGGGCCGCATCATCGGCGACTACCGGCGGGTACCTCTCTATGGCGTGGATCGGCTGATTGAGG
ACAAGGAGGCGCAGAAGGCCAGCCTAGAGGTGGACACGATGGATGAAGAAACCATCCGCCTGCGGGAGGAGCTCTCAGAG
CAGATCAAAGCCCTGTACGAGCTCAAGGAGATGGGGGCCAGCTACGGCTTTGACCTGGGCCGTCCGGCCAGCAACGCCCG
CGAGGCCATCCAGTGGCTGTACCTGGCCTACCTGGCAGCCGTCAAAGAGCAAAATGGCGCTGCCATGTCCCTGGGGCGGG
TTTCCACGTTCCTAGACATCTACATCGAGCGGGATCTGCAGGCCGGGATCCTCACCGAGGAAGAGGCCCAAGAGCTCATC
GACCACTTTGTCATGAAGCTGCGCATGGTCAGGTTTTTGCGCACGCCGGAGTACAACGAGCTCTTTAGCGGGGATCCCAC
CTGGGTTACCGAGTGCATCGGCGGCATGGGGCTGGATGGGCGGCCTCTGGTTACCAAGACCAGCTTCCGCATGCTGCATA
CCCTCTACAACCTGGGGCCGGCACCGGAGCCCAACCTGACGGTGCTCTGGTCGGAGCGGCTGCCGGAAGCCTTCAAGCGC
TACTGCGCCAAGGTCTCCATTGAAACCAGCTCCATCCAGTACGAAAACGACGACTTGATGCGCCCCTACTGGGGGGATGA
CTACGGCATCGCCTGCTGTGTGTCGGCCATGCGCATCGGCAAGCAGATGCAGTTTTTTGGAGCGCGGGTCAACCTGGCCA
AGTGCCTACTCTATGCCATCAATGGCGGCCGGGACGAGATCTCCGGCGAGCAGGTGGCCCCGGCCTATGCCCCCATTACT
TCTGAGGTGCTGGAGTATGACGAGGTGTGGCCGCGCCTGGTGCAGATGATGGCCTGGCTGGCCAAGACCTACGTCAACAC
CATGAACATCATCCACTACATGCACGACAAGTACTGCTACGAGCGCCTGGAGATGGCCCTGCACGACCGGGACGTGCTGC
GCACCATGGCCTTTGGCCTGGCCGGCCTGTCGGTAACAGCCGACTCCCTATCCGCCATCAAATACGCGCGGGTACGGGCC
ATCCGGGATGAGCGGGGCCTAGTGGTGGATTACGCGGTGGATGGCGACTTCCCCAAATACGGCAACAACGATGACCGGGT
TGACTCCATTGCGGTGCAACTGGTGCAGACCTTCATGGCCGAGCTGCGCAAGCACAAGACCTACCGCAATGCCATCCCCA
CCCAGTCCATCCTCACCATCACCTCCAACGTGGTCTACGGCAAGAAAACCGGCAACACCCCCGACGGGCGGCGAGCCGGC
GAGCCCTTTGCCCCAGGGGCCAACCCCATGCACGGCCGGGACACCAAGGGGGCGGTGGCCTCGCTGGCTTCGGTGGCCAA
GCTGCCCTACGACGACGCCCTGGATGGCATCTCCAACACCTTCTCGATTGTGCCGGCGGCCCTAGGTCGCACCGCCGAGG
AGCGGGTTGCTAACCTGGTGGGGCTGCTGGACGGCTACATGCGGGATGGCGGCTTTCACCTCAACGTCAACGTCCTCAAC
CGCGAGACCCTGCTGCAGGCCATGGAGCACCCCGAGCTCTATCCCCAGCTCACCATCCGAGTGTCTGGCTACGCGGTGAA
CTTCATCAAGCTCACCCGCGAGCAACAACTGGATGTGATTAACCGCACCTTCCACCAACACTTCTAA

Upstream 100 bases:

>100_bases
GCAAAGATTGTTGTAGCTTTTGGCAGCTGCTGAGGCGTCGCCTCGCCCTGTCGCCAGCCTATTGGCGGAGTTGTTAGGCG
TAATAGCCGGGAGGATTGTT

Downstream 100 bases:

>100_bases
CGGAAAACGGCGGAGTGGGGGATCCCGGAAGGCTGCCCGTAGCAGGGTCGAGGGAAGGGATCCCCCCTCCCCAAGCAAGG
AGGTTCGATGAGCTATAGCG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 768; Mature: 768

Protein sequence:

>768_residues
MIASAQRSVALSTESKAQKTTERKVSDQTIGRDFLPGRWQRCIDVRDFIQRNYTPYTGDESFLAPASERTQRLWAKVKDL
MALERERGVLDADTDLPSSITSHPPGYIDRELEQIVGLQTDKPLKRAIMPFGGIRMVETALKAYGYELDPKVKEIFTKYR
KTHNDGVFDAYTEEMRRCRRSGIITGLPDAYGRGRIIGDYRRVPLYGVDRLIEDKEAQKASLEVDTMDEETIRLREELSE
QIKALYELKEMGASYGFDLGRPASNAREAIQWLYLAYLAAVKEQNGAAMSLGRVSTFLDIYIERDLQAGILTEEEAQELI
DHFVMKLRMVRFLRTPEYNELFSGDPTWVTECIGGMGLDGRPLVTKTSFRMLHTLYNLGPAPEPNLTVLWSERLPEAFKR
YCAKVSIETSSIQYENDDLMRPYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKCLLYAINGGRDEISGEQVAPAYAPIT
SEVLEYDEVWPRLVQMMAWLAKTYVNTMNIIHYMHDKYCYERLEMALHDRDVLRTMAFGLAGLSVTADSLSAIKYARVRA
IRDERGLVVDYAVDGDFPKYGNNDDRVDSIAVQLVQTFMAELRKHKTYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAG
EPFAPGANPMHGRDTKGAVASLASVAKLPYDDALDGISNTFSIVPAALGRTAEERVANLVGLLDGYMRDGGFHLNVNVLN
RETLLQAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHQHF

Sequences:

>Translated_768_residues
MIASAQRSVALSTESKAQKTTERKVSDQTIGRDFLPGRWQRCIDVRDFIQRNYTPYTGDESFLAPASERTQRLWAKVKDL
MALERERGVLDADTDLPSSITSHPPGYIDRELEQIVGLQTDKPLKRAIMPFGGIRMVETALKAYGYELDPKVKEIFTKYR
KTHNDGVFDAYTEEMRRCRRSGIITGLPDAYGRGRIIGDYRRVPLYGVDRLIEDKEAQKASLEVDTMDEETIRLREELSE
QIKALYELKEMGASYGFDLGRPASNAREAIQWLYLAYLAAVKEQNGAAMSLGRVSTFLDIYIERDLQAGILTEEEAQELI
DHFVMKLRMVRFLRTPEYNELFSGDPTWVTECIGGMGLDGRPLVTKTSFRMLHTLYNLGPAPEPNLTVLWSERLPEAFKR
YCAKVSIETSSIQYENDDLMRPYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKCLLYAINGGRDEISGEQVAPAYAPIT
SEVLEYDEVWPRLVQMMAWLAKTYVNTMNIIHYMHDKYCYERLEMALHDRDVLRTMAFGLAGLSVTADSLSAIKYARVRA
IRDERGLVVDYAVDGDFPKYGNNDDRVDSIAVQLVQTFMAELRKHKTYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAG
EPFAPGANPMHGRDTKGAVASLASVAKLPYDDALDGISNTFSIVPAALGRTAEERVANLVGLLDGYMRDGGFHLNVNVLN
RETLLQAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHQHF
>Mature_768_residues
MIASAQRSVALSTESKAQKTTERKVSDQTIGRDFLPGRWQRCIDVRDFIQRNYTPYTGDESFLAPASERTQRLWAKVKDL
MALERERGVLDADTDLPSSITSHPPGYIDRELEQIVGLQTDKPLKRAIMPFGGIRMVETALKAYGYELDPKVKEIFTKYR
KTHNDGVFDAYTEEMRRCRRSGIITGLPDAYGRGRIIGDYRRVPLYGVDRLIEDKEAQKASLEVDTMDEETIRLREELSE
QIKALYELKEMGASYGFDLGRPASNAREAIQWLYLAYLAAVKEQNGAAMSLGRVSTFLDIYIERDLQAGILTEEEAQELI
DHFVMKLRMVRFLRTPEYNELFSGDPTWVTECIGGMGLDGRPLVTKTSFRMLHTLYNLGPAPEPNLTVLWSERLPEAFKR
YCAKVSIETSSIQYENDDLMRPYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKCLLYAINGGRDEISGEQVAPAYAPIT
SEVLEYDEVWPRLVQMMAWLAKTYVNTMNIIHYMHDKYCYERLEMALHDRDVLRTMAFGLAGLSVTADSLSAIKYARVRA
IRDERGLVVDYAVDGDFPKYGNNDDRVDSIAVQLVQTFMAELRKHKTYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAG
EPFAPGANPMHGRDTKGAVASLASVAKLPYDDALDGISNTFSIVPAALGRTAEERVANLVGLLDGYMRDGGFHLNVNVLN
RETLLQAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHQHF

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=744, Percent_Identity=67.741935483871, Blast_Score=1048, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=758, Percent_Identity=65.5672823218997, Blast_Score=1016, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=577, Percent_Identity=27.7296360485269, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI1790388, Length=679, Percent_Identity=25.1840942562592, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788933, Length=60, Percent_Identity=70, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 86896; Mature: 86896

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIASAQRSVALSTESKAQKTTERKVSDQTIGRDFLPGRWQRCIDVRDFIQRNYTPYTGDE
CCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
SFLAPASERTQRLWAKVKDLMALERERGVLDADTDLPSSITSHPPGYIDRELEQIVGLQT
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCC
DKPLKRAIMPFGGIRMVETALKAYGYELDPKVKEIFTKYRKTHNDGVFDAYTEEMRRCRR
CCHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SGIITGLPDAYGRGRIIGDYRRVPLYGVDRLIEDKEAQKASLEVDTMDEETIRLREELSE
CCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHH
QIKALYELKEMGASYGFDLGRPASNAREAIQWLYLAYLAAVKEQNGAAMSLGRVSTFLDI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
YIERDLQAGILTEEEAQELIDHFVMKLRMVRFLRTPEYNELFSGDPTWVTECIGGMGLDG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHCCCCCCC
RPLVTKTSFRMLHTLYNLGPAPEPNLTVLWSERLPEAFKRYCAKVSIETSSIQYENDDLM
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHEEEECCCEEECCCCCC
RPYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKCLLYAINGGRDEISGEQVAPAYAPIT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
SEVLEYDEVWPRLVQMMAWLAKTYVNTMNIIHYMHDKYCYERLEMALHDRDVLRTMAFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLSVTADSLSAIKYARVRAIRDERGLVVDYAVDGDFPKYGNNDDRVDSIAVQLVQTFMA
HCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
ELRKHKTYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAVA
HHHHHHHHHHCCCCHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
SLASVAKLPYDDALDGISNTFSIVPAALGRTAEERVANLVGLLDGYMRDGGFHLNVNVLN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECC
RETLLQAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHQHF
HHHHHHHHHCCCCCCEEEEEEECEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIASAQRSVALSTESKAQKTTERKVSDQTIGRDFLPGRWQRCIDVRDFIQRNYTPYTGDE
CCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCC
SFLAPASERTQRLWAKVKDLMALERERGVLDADTDLPSSITSHPPGYIDRELEQIVGLQT
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCCC
DKPLKRAIMPFGGIRMVETALKAYGYELDPKVKEIFTKYRKTHNDGVFDAYTEEMRRCRR
CCHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
SGIITGLPDAYGRGRIIGDYRRVPLYGVDRLIEDKEAQKASLEVDTMDEETIRLREELSE
CCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHH
QIKALYELKEMGASYGFDLGRPASNAREAIQWLYLAYLAAVKEQNGAAMSLGRVSTFLDI
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHH
YIERDLQAGILTEEEAQELIDHFVMKLRMVRFLRTPEYNELFSGDPTWVTECIGGMGLDG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHCCCCCCC
RPLVTKTSFRMLHTLYNLGPAPEPNLTVLWSERLPEAFKRYCAKVSIETSSIQYENDDLM
CCCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHEEEECCCEEECCCCCC
RPYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKCLLYAINGGRDEISGEQVAPAYAPIT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
SEVLEYDEVWPRLVQMMAWLAKTYVNTMNIIHYMHDKYCYERLEMALHDRDVLRTMAFGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGLSVTADSLSAIKYARVRAIRDERGLVVDYAVDGDFPKYGNNDDRVDSIAVQLVQTFMA
HCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
ELRKHKTYRNAIPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGRDTKGAVA
HHHHHHHHHHCCCCHHHHHHHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
SLASVAKLPYDDALDGISNTFSIVPAALGRTAEERVANLVGLLDGYMRDGGFHLNVNVLN
HHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECC
RETLLQAMEHPELYPQLTIRVSGYAVNFIKLTREQQLDVINRTFHQHF
HHHHHHHHHCCCCCCEEEEEEECEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA