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The map label for this gene is pflA

Identifier: 86605193

GI number: 86605193

Start: 492370

End: 493122

Strand: Reverse

Name: pflA

Synonym: CYA_0475

Alternate gene names: 86605193

Gene position: 493122-492370 (Counterclockwise)

Preceding gene: 86605194

Following gene: 86605192

Centisome position: 16.81

GC content: 58.43

Gene sequence:

>753_bases
ATGGATACCCCGCTCAACCCCTTGACGCCGATAACCGGCCGCATTCACTCGGTCGAGACCTGTGGCACGGTAGATGGGCC
AGGGATCCGCTTTGTCATCTTTCTGCAGGGCTGTCCTCTGCGCTGTCTGTACTGCCACAATCCCGACTGTCGGGATCCCA
ATGCCGGCCAGGTGGTGACGGTGGATTCCCTCATGGCGGAGATCCAGCGCTGCCGCAACTACTACCTCAAGGGCGGGGGA
GTCACGGCCAGCGGGGGGGAACCGTTGATGCAGCCCAACTTTGTGGCGGAGATTTTCCGCCGCTGCCATGAGCTGGACTT
GCACACGGCTCTAGATACTTCCGGCTATGCGCCCTTGGGCGTGGCCAAGCCGGTGTTGGCCCATACAGACTTGGTGCTAC
TGGATATCAAGTCCTATCTGCCGGAGCTCTATCGCAAAGTTACCGGCGTTTCGCTGCAGCCCACTCTCGATCTGGCTCGC
TATTTGGATCAGATTCACAAGCCCACCTGGATTCGTTTTGTGCTGGTTCCCGGGCTAACAGATCCCGAGGAAAACATCAA
GGGCTTGGCCGAGTTTGTGGCCACGCTCTCCAACGTGGAGCGGGTGGAAGTTTTGCCCTTTCACAAGATGGGGGAATACA
AATGGCAGCAGTTGGGATTGCCCTATACCCTCAGCGATGTTCAGCCGCCCACCCCGGAACAGATCAACCACGCTTTGCAG
ATCTTTCGCGAGCGGGGATTGGTGGCAGTTTAG

Upstream 100 bases:

>100_bases
TCAAAAGAGACAAAACCATGGTGTGCGCAGCATAGACAGCAATTGGGAAGAAAACCTCAAGATTAATTGATATTTTGCAA
AAGCTGTTGAGGATAGAAGT

Downstream 100 bases:

>100_bases
TTTATCGCTGGAGCGACAGCAACAAAACTAGAGCCAATTCTGACTTGAGCCAGCCTGATCCCAAAGTCATCTCTAAAGTC
CTTAGGCTGTCTCCAAGGTA

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MDTPLNPLTPITGRIHSVETCGTVDGPGIRFVIFLQGCPLRCLYCHNPDCRDPNAGQVVTVDSLMAEIQRCRNYYLKGGG
VTASGGEPLMQPNFVAEIFRRCHELDLHTALDTSGYAPLGVAKPVLAHTDLVLLDIKSYLPELYRKVTGVSLQPTLDLAR
YLDQIHKPTWIRFVLVPGLTDPEENIKGLAEFVATLSNVERVEVLPFHKMGEYKWQQLGLPYTLSDVQPPTPEQINHALQ
IFRERGLVAV

Sequences:

>Translated_250_residues
MDTPLNPLTPITGRIHSVETCGTVDGPGIRFVIFLQGCPLRCLYCHNPDCRDPNAGQVVTVDSLMAEIQRCRNYYLKGGG
VTASGGEPLMQPNFVAEIFRRCHELDLHTALDTSGYAPLGVAKPVLAHTDLVLLDIKSYLPELYRKVTGVSLQPTLDLAR
YLDQIHKPTWIRFVLVPGLTDPEENIKGLAEFVATLSNVERVEVLPFHKMGEYKWQQLGLPYTLSDVQPPTPEQINHALQ
IFRERGLVAV
>Mature_250_residues
MDTPLNPLTPITGRIHSVETCGTVDGPGIRFVIFLQGCPLRCLYCHNPDCRDPNAGQVVTVDSLMAEIQRCRNYYLKGGG
VTASGGEPLMQPNFVAEIFRRCHELDLHTALDTSGYAPLGVAKPVLAHTDLVLLDIKSYLPELYRKVTGVSLQPTLDLAR
YLDQIHKPTWIRFVLVPGLTDPEENIKGLAEFVATLSNVERVEVLPFHKMGEYKWQQLGLPYTLSDVQPPTPEQINHALQ
IFRERGLVAV

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=242, Percent_Identity=45.4545454545455, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1790389, Length=266, Percent_Identity=28.1954887218045, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI226510931, Length=196, Percent_Identity=30.6122448979592, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27842; Mature: 27842

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPLNPLTPITGRIHSVETCGTVDGPGIRFVIFLQGCPLRCLYCHNPDCRDPNAGQVVT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCEEE
VDSLMAEIQRCRNYYLKGGGVTASGGEPLMQPNFVAEIFRRCHELDLHTALDTSGYAPLG
HHHHHHHHHHHHHHEECCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VAKPVLAHTDLVLLDIKSYLPELYRKVTGVSLQPTLDLARYLDQIHKPTWIRFVLVPGLT
HHCHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCC
DPEENIKGLAEFVATLSNVERVEVLPFHKMGEYKWQQLGLPYTLSDVQPPTPEQINHALQ
CHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCHHHHCCCCEEECCCCCCCHHHHHHHHH
IFRERGLVAV
HHHHCCCCCC
>Mature Secondary Structure
MDTPLNPLTPITGRIHSVETCGTVDGPGIRFVIFLQGCPLRCLYCHNPDCRDPNAGQVVT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCEEE
VDSLMAEIQRCRNYYLKGGGVTASGGEPLMQPNFVAEIFRRCHELDLHTALDTSGYAPLG
HHHHHHHHHHHHHHEECCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VAKPVLAHTDLVLLDIKSYLPELYRKVTGVSLQPTLDLARYLDQIHKPTWIRFVLVPGLT
HHCHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCC
DPEENIKGLAEFVATLSNVERVEVLPFHKMGEYKWQQLGLPYTLSDVQPPTPEQINHALQ
CHHHHHHHHHHHHHHHHCCCEEEECCCHHCCCCHHHHCCCCEEECCCCCCCHHHHHHHHH
IFRERGLVAV
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]