Click here to switch to the map view.

The map label for this gene is livJ [H]

Identifier: 86604948

GI number: 86604948

Start: 227551

End: 228951

Strand: Reverse

Name: livJ [H]

Synonym: CYA_0224

Alternate gene names: 86604948

Gene position: 228951-227551 (Counterclockwise)

Preceding gene: 86604949

Following gene: 86604947

Centisome position: 7.81

GC content: 65.88

Gene sequence:

>1401_bases
ATGGCTGCGAAGTCTCCACCCCCTATCGTCTACATTGCCCTAGGGCTATTGCTCCTCTGGGGTGGTCATCGCGCCTGGCA
GGCGGGCTACATCCGCTGGCCAGAAGGGATCCCTCTGCCAGGGTTGGGCGGTAGGGCCCAAGATCTGCTCTTAACCAGCG
GCGAGCGCACCCTGTTTGCCGATAGCAGTCCCGCCGCCGTGGCTGCAGCAGAGCTGTTGCGCCAGGGCAATTTCGGCGCG
GCGATCCCGCTCCTAGAGATGGGGCTACAGAACAACCGCAACGACCCGGAAGCTCGCATTTTCCTCAACAACGCCCGCGC
CCAGCAGCAGGGCAACCCCTTGAGCATAGCCGTGGTGGTGCCCATCTCCAGCAACCCCAACCGCGCCAAGGAGACCCTGC
GCGGCGTGGCCCAGGCCCAAGAGGAGCTATTTCAGCAAGGGGGTATTGCAGGGCGGCTGCTGCGGGTGGTGATTGCCGAC
GACGGGGATAACCCAGAGCGAGCGGCGGCAGTGGCCAAAGCCCTGGTGGGCCAGCCCGAGATCCTGGGGGTGATCGGCCA
CAACGCCAGCGATGCCACCCTGGCGGCGGCTGAGATCTATCAGTCTGCCGGCCTGGCCATGATCTCCTCCACCTCCACCA
GCACCCGCATCAGCCTGCTGGGGAACGCCATCTTTCGCACCGTCCCCTCCGACCAGATTGCTGGCACGCAACTGGCCCGC
TATGTCCTTAACAAACTGCGCCTGCAGAGGGTGGCCATCTTCTACAACCCCAACAGCGCCTACAGCACCAGCCTGCGCGA
CGCCTTTGCCGCTACCCTGACCAGCGAAGGGGGCGTTGTGCTGGCGACCTTCGACCTGTCGGCAGCCGATTTCAACCCCC
AGGCCAGCCTGCAGACAGCTCTGGGGCAGGGATCTCAGGCGGCTTTCTTGGCGCCCACGGCTTCCGTGGCCGAAAAAGCG
CTGGCCGTTGCCCGCGAGAACGCTGCCCTTGGCCAGCCCCTCGCCCTGGTGGGGGGGGATGTGCTGTTTAGTCTCACCAC
CCTCAAGGAAGGGGGGGCAGCAGTAGAGGGGCTGGTGGTGGCGGTGCCCTGGCATCCCGACGTGCCCCGCAACCGCGGCT
TTGCCCAGAAGGCAGCCCAGATGTGGGGAGGGCAGGTGAGTTGGCGCACGGCACTGGCCTACGACGCCACCCGTGTTTTT
CTCCAGGCTATTCGGCAGGGATCCCCCAGCCGCAGCGGCATTCTCACTGCCCTGGGCGACCCCAACTTCCGCTTGCCAGA
CGGCGCTTCCGGGGAGGTGCGCTTTTTGCCCTCCGGCGACCGCGATGCTCCCCATGTGCTGGTGCGCATTGAACGGGGCA
ACCGCTCCGGCACCGGCTACGATTTTGTGCCCCTCCCCTAG

Upstream 100 bases:

>100_bases
AGTTCTTGAAGATCCTTGAAGAGGGGGGGTCCAAGAGCTTCCTGAAGGCGTGAGCGGTACCCCTGCTGTCCGGATTCTGC
TGTTCTCTTCCTTGGGGCCC

Downstream 100 bases:

>100_bases
GGCAGGCGCTACAGTAGGGGGTGCTCTCCATCTCCTCAGCTTGGACAGATCCCATGCCCAGCCGGATCACCGCGTGTTTT
CAGGCGCTGCGCAGCCGCGG

Product: hydrophobic amino acid uptake ABC transporter amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-BP [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAAKSPPPIVYIALGLLLLWGGHRAWQAGYIRWPEGIPLPGLGGRAQDLLLTSGERTLFADSSPAAVAAAELLRQGNFGA
AIPLLEMGLQNNRNDPEARIFLNNARAQQQGNPLSIAVVVPISSNPNRAKETLRGVAQAQEELFQQGGIAGRLLRVVIAD
DGDNPERAAAVAKALVGQPEILGVIGHNASDATLAAAEIYQSAGLAMISSTSTSTRISLLGNAIFRTVPSDQIAGTQLAR
YVLNKLRLQRVAIFYNPNSAYSTSLRDAFAATLTSEGGVVLATFDLSAADFNPQASLQTALGQGSQAAFLAPTASVAEKA
LAVARENAALGQPLALVGGDVLFSLTTLKEGGAAVEGLVVAVPWHPDVPRNRGFAQKAAQMWGGQVSWRTALAYDATRVF
LQAIRQGSPSRSGILTALGDPNFRLPDGASGEVRFLPSGDRDAPHVLVRIERGNRSGTGYDFVPLP

Sequences:

>Translated_466_residues
MAAKSPPPIVYIALGLLLLWGGHRAWQAGYIRWPEGIPLPGLGGRAQDLLLTSGERTLFADSSPAAVAAAELLRQGNFGA
AIPLLEMGLQNNRNDPEARIFLNNARAQQQGNPLSIAVVVPISSNPNRAKETLRGVAQAQEELFQQGGIAGRLLRVVIAD
DGDNPERAAAVAKALVGQPEILGVIGHNASDATLAAAEIYQSAGLAMISSTSTSTRISLLGNAIFRTVPSDQIAGTQLAR
YVLNKLRLQRVAIFYNPNSAYSTSLRDAFAATLTSEGGVVLATFDLSAADFNPQASLQTALGQGSQAAFLAPTASVAEKA
LAVARENAALGQPLALVGGDVLFSLTTLKEGGAAVEGLVVAVPWHPDVPRNRGFAQKAAQMWGGQVSWRTALAYDATRVF
LQAIRQGSPSRSGILTALGDPNFRLPDGASGEVRFLPSGDRDAPHVLVRIERGNRSGTGYDFVPLP
>Mature_465_residues
AAKSPPPIVYIALGLLLLWGGHRAWQAGYIRWPEGIPLPGLGGRAQDLLLTSGERTLFADSSPAAVAAAELLRQGNFGAA
IPLLEMGLQNNRNDPEARIFLNNARAQQQGNPLSIAVVVPISSNPNRAKETLRGVAQAQEELFQQGGIAGRLLRVVIADD
GDNPERAAAVAKALVGQPEILGVIGHNASDATLAAAEIYQSAGLAMISSTSTSTRISLLGNAIFRTVPSDQIAGTQLARY
VLNKLRLQRVAIFYNPNSAYSTSLRDAFAATLTSEGGVVLATFDLSAADFNPQASLQTALGQGSQAAFLAPTASVAEKAL
AVARENAALGQPLALVGGDVLFSLTTLKEGGAAVEGLVVAVPWHPDVPRNRGFAQKAAQMWGGQVSWRTALAYDATRVFL
QAIRQGSPSRSGILTALGDPNFRLPDGASGEVRFLPSGDRDAPHVLVRIERGNRSGTGYDFVPLP

Specific function: This protein is a component of the leucine, isoleucine, valine, (threonine) transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=244, Percent_Identity=29.0983606557377, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789867, Length=125, Percent_Identity=36, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 48943; Mature: 48812

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKSPPPIVYIALGLLLLWGGHRAWQAGYIRWPEGIPLPGLGGRAQDLLLTSGERTLFA
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCEEEEE
DSSPAAVAAAELLRQGNFGAAIPLLEMGLQNNRNDPEARIFLNNARAQQQGNPLSIAVVV
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHCCCCCEEEEEEE
PISSNPNRAKETLRGVAQAQEELFQQGGIAGRLLRVVIADDGDNPERAAAVAKALVGQPE
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCC
ILGVIGHNASDATLAAAEIYQSAGLAMISSTSTSTRISLLGNAIFRTVPSDQIAGTQLAR
EEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
YVLNKLRLQRVAIFYNPNSAYSTSLRDAFAATLTSEGGVVLATFDLSAADFNPQASLQTA
HHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHH
LGQGSQAAFLAPTASVAEKALAVARENAALGQPLALVGGDVLFSLTTLKEGGAAVEGLVV
HCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEEEECCCCCCEEEEEEE
AVPWHPDVPRNRGFAQKAAQMWGGQVSWRTALAYDATRVFLQAIRQGSPSRSGILTALGD
EECCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
PNFRLPDGASGEVRFLPSGDRDAPHVLVRIERGNRSGTGYDFVPLP
CCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCEEECCCC
>Mature Secondary Structure 
AAKSPPPIVYIALGLLLLWGGHRAWQAGYIRWPEGIPLPGLGGRAQDLLLTSGERTLFA
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCEEEEECCCEEEEE
DSSPAAVAAAELLRQGNFGAAIPLLEMGLQNNRNDPEARIFLNNARAQQQGNPLSIAVVV
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCHHHCCCCCEEEEEEE
PISSNPNRAKETLRGVAQAQEELFQQGGIAGRLLRVVIADDGDNPERAAAVAKALVGQPE
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHCCCCC
ILGVIGHNASDATLAAAEIYQSAGLAMISSTSTSTRISLLGNAIFRTVPSDQIAGTQLAR
EEEEECCCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
YVLNKLRLQRVAIFYNPNSAYSTSLRDAFAATLTSEGGVVLATFDLSAADFNPQASLQTA
HHHHHHCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHH
LGQGSQAAFLAPTASVAEKALAVARENAALGQPLALVGGDVLFSLTTLKEGGAAVEGLVV
HCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCHHHCCCCCEEEEEECCCCCCEEEEEEE
AVPWHPDVPRNRGFAQKAAQMWGGQVSWRTALAYDATRVFLQAIRQGSPSRSGILTALGD
EECCCCCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEECC
PNFRLPDGASGEVRFLPSGDRDAPHVLVRIERGNRSGTGYDFVPLP
CCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA