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The map label for this gene is trpA

Identifier: 86604947

GI number: 86604947

Start: 226682

End: 227497

Strand: Reverse

Name: trpA

Synonym: CYA_0223

Alternate gene names: 86604947

Gene position: 227497-226682 (Counterclockwise)

Preceding gene: 86604948

Following gene: 86604945

Centisome position: 7.76

GC content: 66.79

Gene sequence:

>816_bases
ATGCCCAGCCGGATCACCGCGTGTTTTCAGGCGCTGCGCAGCCGCGGCGAAGCCGCCCTCATCCCCTACCTCACCGCCGG
GGATCCCAACTTGGAAACCACCTTTGCGGCCCTGCAAGTTTTGGAGGAGAACGGCGCCGACCTCATCGAGTTGGGGGTGC
CCTATTCGGATCCCCTGGCCGATGGGCCGGTGATCCAGGCAGCAGCCACCCGCGCCCTCAGCCGGGGCGTCACCTTGGGC
GACATCTTGCAGGGCTTGCGGGGCCTCTCGCTGCGGGCTCCCCTCGTCCTGTTCACCTACTACAACCCCATCCACCAGCT
CGGCATCCCCACCTTTGTGCGGCAACTGCGCCAGGCCGGCTGTGCCGGCTTGGTCGTCCCCGACCTGCCCCTGGAAGAGG
CCGGATCCCTGCGGGAAGCAACGCAGGCGGAGGGGATCGACCTCATCCTGCTGGTGGCCCCCACCAGCCCCGCCGAGCGA
ATGCGCCGTATTGCCCAACAGTCCAGCGGCTTTATCTACCTGGTCAGCACCACCGGCGTCACCGGCGAACGGGCCACCCT
GGAGCAGCGGATCCCGGCCTTGCTGCAGCAACTGCGCCAATACACCTCCCTGCCGATTGGGGTAGGCTTCGGCATCTCCA
CCCCCGACCAGGCCCGCCAGATTGCTCTCTGGGGAGCGGATGCGGCGATCGTGGGCAGTGCCTTTGTGCAACGGCTGGCC
AAGGCCCCCACCCCCGCCCAAGGGCTGCAACAAATTGCCCACTTCTGCCGGGATCTCAAAGCTGCCCTGCAAGGCATAGG
TACCCCGCAACATTGA

Upstream 100 bases:

>100_bases
GGGGCAACCGCTCCGGCACCGGCTACGATTTTGTGCCCCTCCCCTAGGGCAGGCGCTACAGTAGGGGGTGCTCTCCATCT
CCTCAGCTTGGACAGATCCC

Downstream 100 bases:

>100_bases
CTGCTGCAGTGTGTGGGCAAGAGGCTTGCCTTTATCTCAAAACAAGTGAAACGAATGTTTACATTTTTTTACTGCCCTCG
CCGACCAGTTTCTGGCCTGC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MPSRITACFQALRSRGEAALIPYLTAGDPNLETTFAALQVLEENGADLIELGVPYSDPLADGPVIQAAATRALSRGVTLG
DILQGLRGLSLRAPLVLFTYYNPIHQLGIPTFVRQLRQAGCAGLVVPDLPLEEAGSLREATQAEGIDLILLVAPTSPAER
MRRIAQQSSGFIYLVSTTGVTGERATLEQRIPALLQQLRQYTSLPIGVGFGISTPDQARQIALWGADAAIVGSAFVQRLA
KAPTPAQGLQQIAHFCRDLKAALQGIGTPQH

Sequences:

>Translated_271_residues
MPSRITACFQALRSRGEAALIPYLTAGDPNLETTFAALQVLEENGADLIELGVPYSDPLADGPVIQAAATRALSRGVTLG
DILQGLRGLSLRAPLVLFTYYNPIHQLGIPTFVRQLRQAGCAGLVVPDLPLEEAGSLREATQAEGIDLILLVAPTSPAER
MRRIAQQSSGFIYLVSTTGVTGERATLEQRIPALLQQLRQYTSLPIGVGFGISTPDQARQIALWGADAAIVGSAFVQRLA
KAPTPAQGLQQIAHFCRDLKAALQGIGTPQH
>Mature_270_residues
PSRITACFQALRSRGEAALIPYLTAGDPNLETTFAALQVLEENGADLIELGVPYSDPLADGPVIQAAATRALSRGVTLGD
ILQGLRGLSLRAPLVLFTYYNPIHQLGIPTFVRQLRQAGCAGLVVPDLPLEEAGSLREATQAEGIDLILLVAPTSPAERM
RRIAQQSSGFIYLVSTTGVTGERATLEQRIPALLQQLRQYTSLPIGVGFGISTPDQARQIALWGADAAIVGSAFVQRLAK
APTPAQGLQQIAHFCRDLKAALQGIGTPQH

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=264, Percent_Identity=35.2272727272727, Blast_Score=154, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6321412, Length=247, Percent_Identity=35.6275303643725, Blast_Score=165, Evalue=6e-42,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 28824; Mature: 28692

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRITACFQALRSRGEAALIPYLTAGDPNLETTFAALQVLEENGADLIELGVPYSDPLA
CCHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPVIQAAATRALSRGVTLGDILQGLRGLSLRAPLVLFTYYNPIHQLGIPTFVRQLRQAG
CCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCHHHHHHHHHHCC
CAGLVVPDLPLEEAGSLREATQAEGIDLILLVAPTSPAERMRRIAQQSSGFIYLVSTTGV
CCEEECCCCCHHHHCCHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEEECCC
TGERATLEQRIPALLQQLRQYTSLPIGVGFGISTPDQARQIALWGADAAIVGSAFVQRLA
CCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHEEEEECCCHHHHHHHHHHHHH
KAPTPAQGLQQIAHFCRDLKAALQGIGTPQH
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PSRITACFQALRSRGEAALIPYLTAGDPNLETTFAALQVLEENGADLIELGVPYSDPLA
CHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCC
DGPVIQAAATRALSRGVTLGDILQGLRGLSLRAPLVLFTYYNPIHQLGIPTFVRQLRQAG
CCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHCCHHHHHHHHHHCC
CAGLVVPDLPLEEAGSLREATQAEGIDLILLVAPTSPAERMRRIAQQSSGFIYLVSTTGV
CCEEECCCCCHHHHCCHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEEEECCC
TGERATLEQRIPALLQQLRQYTSLPIGVGFGISTPDQARQIALWGADAAIVGSAFVQRLA
CCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHEEEEECCCHHHHHHHHHHHHH
KAPTPAQGLQQIAHFCRDLKAALQGIGTPQH
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA