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The map label for this gene is metE-1

Identifier: 86604877

GI number: 86604877

Start: 142592

End: 144856

Strand: Reverse

Name: metE-1

Synonym: CYA_0148

Alternate gene names: 86604877

Gene position: 144856-142592 (Counterclockwise)

Preceding gene: 86604885

Following gene: 86604874

Centisome position: 4.94

GC content: 58.63

Gene sequence:

>2265_bases
ATGACTGCACAAACCATGACATTGGGGTATGCCCGCATGGGCAAGCGCCGCGAGCTCAAAAAAGCTTTGGAAGGCTTTTG
GAGTGGTGCCCTAGGGTCAGAGGCTCTGCTGGCTACCTTTTGGGATTTGGAGACTCAAGCCTGGCAGACTCAGCTACAAG
CTGGGATTGATCACATTGCGGTAGGGGATCAGACTCTCTACGACCATGTACTGGACTGGGCCACCTGGCTGGGGCTGATC
CCCTCTCGGTTCCGAGGTCTGTCGGGTTTAGACCGCTACTTTGCCATGGCGCGGGGGCGGGAGGGCCTGCCGGCTTTGGA
AATGACCAAGTGGTTTGACACCAACTACCACTACTTGGTACCCGAAATTGAGCCGGAAGCCGATCCCAGTCCTAACTTTG
GGGATTTTTTGGAAAGAGTTCGGCGGGCCCAAGGGATCCTAGGGGAGCGCACCAGCCCCGTTGTCCTCAGTCCAGTGACT
TTGCTCTGCCTTAGCCAGCGATCTGGGGATCTGCGGGCGGACTTGGAAAAGCTCCTTCCTCTGTACCGGGATCTCCTCCA
GGAGTTAAAACAGCTGGGGATCCCAGAAGTCCAGATTCACGATCCCATTTTGGTTACCAGCCAGGGAAGTGGCCTGCGGG
AAGCTGTGGAAATGAGCTACCGCCAGTTGGCAACCGCCGGGATCCGTGTCCACTTGGTCACCTACTTCGATGACCTGGGG
GAGACTTACCCCTGGGTGGTGCAACTGCCGGTGGCCGGCATTAGCCTTGACTTTACCCGTGGCCACACCCTGGATCTGGT
AAGAACCTATGGCTTTCCGGCTGACCAGATCCTAGGTGCTGGCGTAGTGGACGCTCGGAATGTCTGGAAGGTTCAGCCTG
AGACCGTCTTGGCCAGCCTGCGGGAGCTGCAGGGGGTAGCTCCCAACCTACGGGTGCAGCCTTCCGCTTCGTTGCAATTT
GTGCCCCACGACGCTGCTCTGGAAGCCCAGTTGCCGGAACCCCTCAGAAATGTCCTCAGCTTTGCCGAACAGAAGCTGGC
CGAAGTAGCTCTCCTGGCGCGAGCCCTCAACGGCGAAGACACTGCTGCCCAGCAGGCGGAAATCCAGCAGCAGTGGCAGA
GCTTTGCGCAGTTTAGCCCCCCCAACCCCCAAGTGCGCCAGGCTTTGGCCAACCTCAAGCTCCAGGACTTCGAGCGATTC
CTGCCCTACGAGCAGCGCCTAAGTCGGCAGGTTCAGCTACCACCTTTGCCCACCACCACCATTGGCTCCTTCCCCCAAAC
TCCAGAGGTGCGGCAGTGGCGAGCTAAGTACAAAAGAGGGGAGGTTTCGCAGGCGGAGTACGAAGCAGCCATTGACGCTG
AGATTGCCAAGTGCATCCGCATTCAAGAAGAGATTGGCCTGGATGTGTTGGTGCATGGAGAATTTGAGCGCACCGACATG
GTGGAGTACTTTGCCCAGAAGCTGGAAGGCTTTGCCTTCACTGAACACGGCTGGGTGCAGAGTTACGGCAGCCGCTGCGT
GCGCCCTCCCATTCTCTACGGCGACGTGGTTCGCCCCCAACCCATGACAGTGCGGGAATTCCAAGTAGCCCAGTCCCACA
CCCAAAAGCCGGTGAAAGGCATGCTCACGGGGCCAGTGACTATGCTCAACTGGTCCTTTCCCCGTGTTGATATTCCCCGG
CGGGAGCAGGCTTTGCAGATTGCCCTGGCCTTGAGAGCCGAAGTGGCAGATCTGGAAGCGGCTGGAGCGGTGATGGTGCA
AGTGGATGAGCCGGCACTGCGGGAGGGGCTACCTCTGAAAAAAGAACGCTGGCCAGAATACCTCAGCTGGGCAGTGGATG
CCTTTCGCCTAGCTACCGGCGGGGCCAAGCCGGAAACCCAGATTCACACCCACATGTGCTACTCAGAGTTTGGGGACATC
ATCGAGCACATTGAGCGGCTGGATGCCGACGTGCTTTCCATTGAAAACAGCCGCAGCAACAACAAAACCCTGCTGCAAAT
TGCCCAGGCTGGGTATCGTCACCAGGTGGGCAACGGCGTTTACGATGTCCACAGCCCTGCGGTGCCGAGCGTTGAGCAAA
TCCTGCAGCAGCTGCGCACAGGACTGGCCCACTTGCCTGTGGAGCAAACTTGGGTTAATCCCGATTGTGGCCTCAAGACC
CGCCGCTGGGAGGAGGTGATCCCCGCACTGAAAAATATGGTGGCTGCCGCTCATCAGCTAAGGGAGGAGCTTCTGGAAAC
CCAACCTGAGGGTCTGCGCGAGTAA

Upstream 100 bases:

>100_bases
AGAGGAACAGCCGGTTTCTTCCTTGGCGGGCGGGATCCCAGTTGAGGGCGAATTGAACGCAGGAAAATTAGAAAACTCAG
TATTTGGGATGGCACACAAC

Downstream 100 bases:

>100_bases
GGCCCACGACCCGCTGATGTAACTGGTCGGGCTGGCTTGACAAGTCGCCGCGGGCGGTGCCGCTGTTGTGCTGATGCGGC
TGGCTTAGGGCCTACGGTCT

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 754; Mature: 753

Protein sequence:

>754_residues
MTAQTMTLGYARMGKRRELKKALEGFWSGALGSEALLATFWDLETQAWQTQLQAGIDHIAVGDQTLYDHVLDWATWLGLI
PSRFRGLSGLDRYFAMARGREGLPALEMTKWFDTNYHYLVPEIEPEADPSPNFGDFLERVRRAQGILGERTSPVVLSPVT
LLCLSQRSGDLRADLEKLLPLYRDLLQELKQLGIPEVQIHDPILVTSQGSGLREAVEMSYRQLATAGIRVHLVTYFDDLG
ETYPWVVQLPVAGISLDFTRGHTLDLVRTYGFPADQILGAGVVDARNVWKVQPETVLASLRELQGVAPNLRVQPSASLQF
VPHDAALEAQLPEPLRNVLSFAEQKLAEVALLARALNGEDTAAQQAEIQQQWQSFAQFSPPNPQVRQALANLKLQDFERF
LPYEQRLSRQVQLPPLPTTTIGSFPQTPEVRQWRAKYKRGEVSQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
VEYFAQKLEGFAFTEHGWVQSYGSRCVRPPILYGDVVRPQPMTVREFQVAQSHTQKPVKGMLTGPVTMLNWSFPRVDIPR
REQALQIALALRAEVADLEAAGAVMVQVDEPALREGLPLKKERWPEYLSWAVDAFRLATGGAKPETQIHTHMCYSEFGDI
IEHIERLDADVLSIENSRSNNKTLLQIAQAGYRHQVGNGVYDVHSPAVPSVEQILQQLRTGLAHLPVEQTWVNPDCGLKT
RRWEEVIPALKNMVAAAHQLREELLETQPEGLRE

Sequences:

>Translated_754_residues
MTAQTMTLGYARMGKRRELKKALEGFWSGALGSEALLATFWDLETQAWQTQLQAGIDHIAVGDQTLYDHVLDWATWLGLI
PSRFRGLSGLDRYFAMARGREGLPALEMTKWFDTNYHYLVPEIEPEADPSPNFGDFLERVRRAQGILGERTSPVVLSPVT
LLCLSQRSGDLRADLEKLLPLYRDLLQELKQLGIPEVQIHDPILVTSQGSGLREAVEMSYRQLATAGIRVHLVTYFDDLG
ETYPWVVQLPVAGISLDFTRGHTLDLVRTYGFPADQILGAGVVDARNVWKVQPETVLASLRELQGVAPNLRVQPSASLQF
VPHDAALEAQLPEPLRNVLSFAEQKLAEVALLARALNGEDTAAQQAEIQQQWQSFAQFSPPNPQVRQALANLKLQDFERF
LPYEQRLSRQVQLPPLPTTTIGSFPQTPEVRQWRAKYKRGEVSQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
VEYFAQKLEGFAFTEHGWVQSYGSRCVRPPILYGDVVRPQPMTVREFQVAQSHTQKPVKGMLTGPVTMLNWSFPRVDIPR
REQALQIALALRAEVADLEAAGAVMVQVDEPALREGLPLKKERWPEYLSWAVDAFRLATGGAKPETQIHTHMCYSEFGDI
IEHIERLDADVLSIENSRSNNKTLLQIAQAGYRHQVGNGVYDVHSPAVPSVEQILQQLRTGLAHLPVEQTWVNPDCGLKT
RRWEEVIPALKNMVAAAHQLREELLETQPEGLRE
>Mature_753_residues
TAQTMTLGYARMGKRRELKKALEGFWSGALGSEALLATFWDLETQAWQTQLQAGIDHIAVGDQTLYDHVLDWATWLGLIP
SRFRGLSGLDRYFAMARGREGLPALEMTKWFDTNYHYLVPEIEPEADPSPNFGDFLERVRRAQGILGERTSPVVLSPVTL
LCLSQRSGDLRADLEKLLPLYRDLLQELKQLGIPEVQIHDPILVTSQGSGLREAVEMSYRQLATAGIRVHLVTYFDDLGE
TYPWVVQLPVAGISLDFTRGHTLDLVRTYGFPADQILGAGVVDARNVWKVQPETVLASLRELQGVAPNLRVQPSASLQFV
PHDAALEAQLPEPLRNVLSFAEQKLAEVALLARALNGEDTAAQQAEIQQQWQSFAQFSPPNPQVRQALANLKLQDFERFL
PYEQRLSRQVQLPPLPTTTIGSFPQTPEVRQWRAKYKRGEVSQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDMV
EYFAQKLEGFAFTEHGWVQSYGSRCVRPPILYGDVVRPQPMTVREFQVAQSHTQKPVKGMLTGPVTMLNWSFPRVDIPRR
EQALQIALALRAEVADLEAAGAVMVQVDEPALREGLPLKKERWPEYLSWAVDAFRLATGGAKPETQIHTHMCYSEFGDII
EHIERLDADVLSIENSRSNNKTLLQIAQAGYRHQVGNGVYDVHSPAVPSVEQILQQLRTGLAHLPVEQTWVNPDCGLKTR
RWEEVIPALKNMVAAAHQLREELLETQPEGLRE

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=758, Percent_Identity=46.5699208443272, Blast_Score=616, Evalue=1e-177,
Organism=Saccharomyces cerevisiae, GI6320936, Length=773, Percent_Identity=42.9495472186287, Blast_Score=567, Evalue=1e-162,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 84836; Mature: 84705

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQTMTLGYARMGKRRELKKALEGFWSGALGSEALLATFWDLETQAWQTQLQAGIDHIA
CCCCEEEHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VGDQTLYDHVLDWATWLGLIPSRFRGLSGLDRYFAMARGREGLPALEMTKWFDTNYHYLV
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEC
PEIEPEADPSPNFGDFLERVRRAQGILGERTSPVVLSPVTLLCLSQRSGDLRADLEKLLP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCCCHHHHHHHHHH
LYRDLLQELKQLGIPEVQIHDPILVTSQGSGLREAVEMSYRQLATAGIRVHLVTYFDDLG
HHHHHHHHHHHCCCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHC
ETYPWVVQLPVAGISLDFTRGHTLDLVRTYGFPADQILGAGVVDARNVWKVQPETVLASL
CCCCEEEEECCCCEEEECCCCCHHHHHHHCCCCHHHHHCCCCCCCCCCEECCHHHHHHHH
RELQGVAPNLRVQPSASLQFVPHDAALEAQLPEPLRNVLSFAEQKLAEVALLARALNGED
HHHHCCCCCCEECCCCCEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAAQQAEIQQQWQSFAQFSPPNPQVRQALANLKLQDFERFLPYEQRLSRQVQLPPLPTTT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHCCCCCCCCCCCCC
IGSFPQTPEVRQWRAKYKRGEVSQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
VEYFAQKLEGFAFTEHGWVQSYGSRCVRPPILYGDVVRPQPMTVREFQVAQSHTQKPVKG
HHHHHHHHCCCEECCCHHHHHHCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCHHH
MLTGPVTMLNWSFPRVDIPRREQALQIALALRAEVADLEAAGAVMVQVDEPALREGLPLK
HHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCC
KERWPEYLSWAVDAFRLATGGAKPETQIHTHMCYSEFGDIIEHIERLDADVLSIENSRSN
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCC
NKTLLQIAQAGYRHQVGNGVYDVHSPAVPSVEQILQQLRTGLAHLPVEQTWVNPDCGLKT
CHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCH
RRWEEVIPALKNMVAAAHQLREELLETQPEGLRE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TAQTMTLGYARMGKRRELKKALEGFWSGALGSEALLATFWDLETQAWQTQLQAGIDHIA
CCCEEEHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
VGDQTLYDHVLDWATWLGLIPSRFRGLSGLDRYFAMARGREGLPALEMTKWFDTNYHYLV
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEC
PEIEPEADPSPNFGDFLERVRRAQGILGERTSPVVLSPVTLLCLSQRSGDLRADLEKLLP
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCCCHHHHHHHHHH
LYRDLLQELKQLGIPEVQIHDPILVTSQGSGLREAVEMSYRQLATAGIRVHLVTYFDDLG
HHHHHHHHHHHCCCCCEEECCCEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHC
ETYPWVVQLPVAGISLDFTRGHTLDLVRTYGFPADQILGAGVVDARNVWKVQPETVLASL
CCCCEEEEECCCCEEEECCCCCHHHHHHHCCCCHHHHHCCCCCCCCCCEECCHHHHHHHH
RELQGVAPNLRVQPSASLQFVPHDAALEAQLPEPLRNVLSFAEQKLAEVALLARALNGED
HHHHCCCCCCEECCCCCEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TAAQQAEIQQQWQSFAQFSPPNPQVRQALANLKLQDFERFLPYEQRLSRQVQLPPLPTTT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHHCCCCCCCCCCCCC
IGSFPQTPEVRQWRAKYKRGEVSQAEYEAAIDAEIAKCIRIQEEIGLDVLVHGEFERTDM
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHH
VEYFAQKLEGFAFTEHGWVQSYGSRCVRPPILYGDVVRPQPMTVREFQVAQSHTQKPVKG
HHHHHHHHCCCEECCCHHHHHHCCCCCCCCCEECCCCCCCCCCHHHHHHHHHHCCCCHHH
MLTGPVTMLNWSFPRVDIPRREQALQIALALRAEVADLEAAGAVMVQVDEPALREGLPLK
HHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCC
KERWPEYLSWAVDAFRLATGGAKPETQIHTHMCYSEFGDIIEHIERLDADVLSIENSRSN
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCC
NKTLLQIAQAGYRHQVGNGVYDVHSPAVPSVEQILQQLRTGLAHLPVEQTWVNPDCGLKT
CHHHHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCH
RRWEEVIPALKNMVAAAHQLREELLETQPEGLRE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA