Click here to switch to the map view.

The map label for this gene is miaA

Identifier: 86604812

GI number: 86604812

Start: 80829

End: 81851

Strand: Reverse

Name: miaA

Synonym: CYA_0081

Alternate gene names: 86604812

Gene position: 81851-80829 (Counterclockwise)

Preceding gene: 86604814

Following gene: 86604810

Centisome position: 2.79

GC content: 64.71

Gene sequence:

>1023_bases
ATGGATCAAAACCGGTCTCCAAATGGGCGCGACTGTCGAGAACCTCCCTCCCCCTCCTCGACTGCAAGGCCGGGGCTTGT
CGTCATCGCTGGCCCCACGGCCACCGGCAAGTCGCGCCAGGCGCTGCTGTTGGCCCAGCGGCTGGGATCCCCCCTGTTGA
ACGCCGACTCTCGTCAGGTGTACCGGGAGTTCGACATCGGCACTGCCAAGCCCACTCCCGCCGAGCGGGCCCTCTGGCCC
CACGAGTTGATTGACTTTGTGGATCCCCGCCACACCTATACAGTGGCCGAGTTTCAGCAGGCGGCCCAGGCGCGCATTGC
CGCAGCCCATCGGCAGGGACAGACTCCTATTCTGGTGGGGGGCACAGGGCTCTACATTCAGTCGATCACGGCAGGCTTAG
GGATCCCGGCAGTTCCCCCCCAGCCTCAGTTGCGCGCTCAACTGGAGACGTGGCCGCCCGAGATCCGCTACGCCTGGCTG
CAGCAGTTGGATCCTGTGGCAGCGGGGCAGATTCACCCTCATGATGAAGTGCGCACCCTGCGGGCTTTGGAGATCATCTA
CACCACTGGGAAGCCGGCTTCCTACCTGCGGCAGGCCCATCCTCCTGACTACCCCATTTTGCTCTTGGGGTTGCACTGCC
CAATGCCCCGTCTGGAACAACGCATTGCCCGGCGCACAGCAGAAATGCTGGCGGCAGGCTGGATTGAGGAGGTAAAAACG
CTGCGGCAGCGCTACGGCCCAGACCTGCCCCTCTTGCAGACCTTGGGCTATGCGGAGATCGGCGCTTACCTGGAAGGGCG
GATCCCGGCAGCCGAGCTGCAGCCTCTGATTGTGCGGCGCACCCGCCAGTTTGCCAAGCGGCAGATGACCTGGTTTCGGG
CCATGCCAGGGATCCAGTGGCTGGACTGCGAGGCCGAGGATCTGCCCGAACAGATCTGGAAGCGGGTTACAGCGTGGATG
GCAGCTCAGACCAGCGCCGGAACCACTCCTGCCGTCGCCGCGCGTCCGCCCGCCGATCCGTAA

Upstream 100 bases:

>100_bases
GGGAAGACGATAGAGACTACGGATGTTCTATTCTAGCCACAGCGCTAAGGTTCTTTGAAAATTTTAATCTGCTGCCCTGG
GTGAACTTTCTTGCCGGCGG

Downstream 100 bases:

>100_bases
GGATCTCCAACACCCGCACCCCTTGGGCGGGCAAGGGATCCAAAGCCTGGGCCAGCTCTTCCCAGGTCTCTATCCGCCGG
TGGGGGATGCCATGGGCCTG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MDQNRSPNGRDCREPPSPSSTARPGLVVIAGPTATGKSRQALLLAQRLGSPLLNADSRQVYREFDIGTAKPTPAERALWP
HELIDFVDPRHTYTVAEFQQAAQARIAAAHRQGQTPILVGGTGLYIQSITAGLGIPAVPPQPQLRAQLETWPPEIRYAWL
QQLDPVAAGQIHPHDEVRTLRALEIIYTTGKPASYLRQAHPPDYPILLLGLHCPMPRLEQRIARRTAEMLAAGWIEEVKT
LRQRYGPDLPLLQTLGYAEIGAYLEGRIPAAELQPLIVRRTRQFAKRQMTWFRAMPGIQWLDCEAEDLPEQIWKRVTAWM
AAQTSAGTTPAVAARPPADP

Sequences:

>Translated_340_residues
MDQNRSPNGRDCREPPSPSSTARPGLVVIAGPTATGKSRQALLLAQRLGSPLLNADSRQVYREFDIGTAKPTPAERALWP
HELIDFVDPRHTYTVAEFQQAAQARIAAAHRQGQTPILVGGTGLYIQSITAGLGIPAVPPQPQLRAQLETWPPEIRYAWL
QQLDPVAAGQIHPHDEVRTLRALEIIYTTGKPASYLRQAHPPDYPILLLGLHCPMPRLEQRIARRTAEMLAAGWIEEVKT
LRQRYGPDLPLLQTLGYAEIGAYLEGRIPAAELQPLIVRRTRQFAKRQMTWFRAMPGIQWLDCEAEDLPEQIWKRVTAWM
AAQTSAGTTPAVAARPPADP
>Mature_340_residues
MDQNRSPNGRDCREPPSPSSTARPGLVVIAGPTATGKSRQALLLAQRLGSPLLNADSRQVYREFDIGTAKPTPAERALWP
HELIDFVDPRHTYTVAEFQQAAQARIAAAHRQGQTPILVGGTGLYIQSITAGLGIPAVPPQPQLRAQLETWPPEIRYAWL
QQLDPVAAGQIHPHDEVRTLRALEIIYTTGKPASYLRQAHPPDYPILLLGLHCPMPRLEQRIARRTAEMLAAGWIEEVKT
LRQRYGPDLPLLQTLGYAEIGAYLEGRIPAAELQPLIVRRTRQFAKRQMTWFRAMPGIQWLDCEAEDLPEQIWKRVTAWM
AAQTSAGTTPAVAARPPADP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=31.8471337579618, Blast_Score=146, Evalue=2e-35,
Organism=Escherichia coli, GI1790613, Length=304, Percent_Identity=39.1447368421053, Blast_Score=184, Evalue=9e-48,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=33.3333333333333, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24649830, Length=265, Percent_Identity=32.0754716981132, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SYNJA (Q2JY05)

Other databases:

- EMBL:   CP000239
- RefSeq:   YP_473575.1
- ProteinModelPortal:   Q2JY05
- SMR:   Q2JY05
- STRING:   Q2JY05
- GeneID:   3897110
- GenomeReviews:   CP000239_GR
- KEGG:   cya:CYA_0081
- TIGR:   CYA_0081
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   CLSK750055
- BioCyc:   SSP321327:CYA_0081-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 37754; Mature: 37754

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQNRSPNGRDCREPPSPSSTARPGLVVIAGPTATGKSRQALLLAQRLGSPLLNADSRQV
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHH
YREFDIGTAKPTPAERALWPHELIDFVDPRHTYTVAEFQQAAQARIAAAHRQGQTPILVG
HHHHCCCCCCCCCHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
GTGLYIQSITAGLGIPAVPPQPQLRAQLETWPPEIRYAWLQQLDPVAAGQIHPHDEVRTL
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHH
RALEIIYTTGKPASYLRQAHPPDYPILLLGLHCPMPRLEQRIARRTAEMLAAGWIEEVKT
HHHEEEEECCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRQRYGPDLPLLQTLGYAEIGAYLEGRIPAAELQPLIVRRTRQFAKRQMTWFRAMPGIQW
HHHHHCCCCHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LDCEAEDLPEQIWKRVTAWMAAQTSAGTTPAVAARPPADP
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDQNRSPNGRDCREPPSPSSTARPGLVVIAGPTATGKSRQALLLAQRLGSPLLNADSRQV
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHH
YREFDIGTAKPTPAERALWPHELIDFVDPRHTYTVAEFQQAAQARIAAAHRQGQTPILVG
HHHHCCCCCCCCCHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
GTGLYIQSITAGLGIPAVPPQPQLRAQLETWPPEIRYAWLQQLDPVAAGQIHPHDEVRTL
CCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHH
RALEIIYTTGKPASYLRQAHPPDYPILLLGLHCPMPRLEQRIARRTAEMLAAGWIEEVKT
HHHEEEEECCCCHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LRQRYGPDLPLLQTLGYAEIGAYLEGRIPAAELQPLIVRRTRQFAKRQMTWFRAMPGIQW
HHHHHCCCCHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LDCEAEDLPEQIWKRVTAWMAAQTSAGTTPAVAARPPADP
ECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA