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The map label for this gene is entA [C]

Identifier: 86361287

GI number: 86361287

Start: 625079

End: 625801

Strand: Reverse

Name: entA [C]

Synonym: RHE_PF00559

Alternate gene names: 86361287

Gene position: 625801-625079 (Counterclockwise)

Preceding gene: 86361289

Following gene: 86361286

Centisome position: 97.4

GC content: 64.04

Gene sequence:

>723_bases
ATGCAGGCGAATATCGATCTTCAAGGACTTCGCGTAGGGATCACCGGCGGCACTTCCGGCCTCGGACTGGCGCTTGTGCG
GCAGCTTGCCGAGAAGGGTGCGAGCGTTGCCTTCGTCGCCCGCACCGCCGCCAATGTCGAACGCATCGCAGCCGAGACGG
GCGCGTTTGGCATTGTCGCCGATATCGGCAAGAAGGAAGACATCTATCCGATCGCGATGCAGATCAGTGCCAATCTTGGC
GGCGTCGACGTCCTGATCAACAATGCATCGAGCCTCGGTCCTGTGCCTCTGGCGCTTCTTGCCGACACGGACTGCGAGGA
GCTGGAAGCGGCCCTCGTAGTCAACGTTCTCGGCGCCTTCCGGCTGACGAAAGCCCTGTTCGGTGCGCTTGCCACGTCGG
CGCGCGAGGGCCGCGGCGCGCTGGTGATCAACATCTCCAGCGACGCGGCGGTCAACGCCTATCCCGGCTGGGGCGCATAC
GGCGCGAGCAAGGCAGCGCTTGCCCATCTGACGGCGATTTGGGACGCGGAGGCGAAAGGAGATGGAATAAGGTTTCTTGC
CCCCGATCCTGGCGACATGGACACCCCGCTGCACGCGCTGGCGCTACCGGACGCCGATCGGTCGGCCTTGAAATATCCGA
AGCTGGCCGCTGCCGAAATCATCGAGAAGATGCTCGACGCGTTGCCGAGTCACGATGCGCTTACCGCCGGAGCCCACGCG
TGA

Upstream 100 bases:

>100_bases
CAGCATCGGATCCATGCATAAATAAAAGCACGAAGCTCTCTTAACTTCAAGTTAACTTGAACTTGTAGCAGTTAGGCGTA
CCCATTGAAGGAGCACCGCA

Downstream 100 bases:

>100_bases
TCGCCGCTGATCGCCTCGATCGGCAATCGGCCAGGCTGCTCGTCGTGGAGGCGGACGGCAGGATGCGCGACCTGCCGCGG
GCTAACCTCGGGATGTTATT

Product: putative short chain dehydrogenase protein

Products: 2,3-Dihydroxybenzoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MQANIDLQGLRVGITGGTSGLGLALVRQLAEKGASVAFVARTAANVERIAAETGAFGIVADIGKKEDIYPIAMQISANLG
GVDVLINNASSLGPVPLALLADTDCEELEAALVVNVLGAFRLTKALFGALATSAREGRGALVINISSDAAVNAYPGWGAY
GASKAALAHLTAIWDAEAKGDGIRFLAPDPGDMDTPLHALALPDADRSALKYPKLAAAEIIEKMLDALPSHDALTAGAHA

Sequences:

>Translated_240_residues
MQANIDLQGLRVGITGGTSGLGLALVRQLAEKGASVAFVARTAANVERIAAETGAFGIVADIGKKEDIYPIAMQISANLG
GVDVLINNASSLGPVPLALLADTDCEELEAALVVNVLGAFRLTKALFGALATSAREGRGALVINISSDAAVNAYPGWGAY
GASKAALAHLTAIWDAEAKGDGIRFLAPDPGDMDTPLHALALPDADRSALKYPKLAAAEIIEKMLDALPSHDALTAGAHA
>Mature_240_residues
MQANIDLQGLRVGITGGTSGLGLALVRQLAEKGASVAFVARTAANVERIAAETGAFGIVADIGKKEDIYPIAMQISANLG
GVDVLINNASSLGPVPLALLADTDCEELEAALVVNVLGAFRLTKALFGALATSAREGRGALVINISSDAAVNAYPGWGAY
GASKAALAHLTAIWDAEAKGDGIRFLAPDPGDMDTPLHALALPDADRSALKYPKLAAAEIIEKMLDALPSHDALTAGAHA

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=230, Percent_Identity=29.1304347826087, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI5031737, Length=200, Percent_Identity=33.5, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI33667109, Length=187, Percent_Identity=33.1550802139037, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4503817, Length=201, Percent_Identity=26.3681592039801, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1786812, Length=195, Percent_Identity=29.7435897435897, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87082100, Length=196, Percent_Identity=27.5510204081633, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787335, Length=226, Percent_Identity=29.2035398230088, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=216, Percent_Identity=33.7962962962963, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322227, Length=192, Percent_Identity=31.25, Blast_Score=77, Evalue=2e-15,
Organism=Drosophila melanogaster, GI23397609, Length=188, Percent_Identity=38.8297872340425, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24644339, Length=222, Percent_Identity=28.8288288288288, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17737361, Length=205, Percent_Identity=29.2682926829268, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24641232, Length=232, Percent_Identity=27.1551724137931, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28571526, Length=213, Percent_Identity=29.1079812206573, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21357041, Length=191, Percent_Identity=29.3193717277487, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007236, Length=206, Percent_Identity=27.6699029126214, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI21355319, Length=232, Percent_Identity=27.5862068965517, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.3.1.28 [C]

Molecular weight: Translated: 24474; Mature: 24474

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQANIDLQGLRVGITGGTSGLGLALVRQLAEKGASVAFVARTAANVERIAAETGAFGIVA
CCCCCEECEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCEEEE
DIGKKEDIYPIAMQISANLGGVDVLINNASSLGPVPLALLADTDCEELEAALVVNVLGAF
CCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
RLTKALFGALATSAREGRGALVINISSDAAVNAYPGWGAYGASKAALAHLTAIWDAEAKG
HHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCC
DGIRFLAPDPGDMDTPLHALALPDADRSALKYPKLAAAEIIEKMLDALPSHDALTAGAHA
CCEEEECCCCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQANIDLQGLRVGITGGTSGLGLALVRQLAEKGASVAFVARTAANVERIAAETGAFGIVA
CCCCCEECEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCEEEE
DIGKKEDIYPIAMQISANLGGVDVLINNASSLGPVPLALLADTDCEELEAALVVNVLGAF
CCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
RLTKALFGALATSAREGRGALVINISSDAAVNAYPGWGAYGASKAALAHLTAIWDAEAKG
HHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCC
DGIRFLAPDPGDMDTPLHALALPDADRSALKYPKLAAAEIIEKMLDALPSHDALTAGAHA
CCEEEECCCCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 5550 [C]

Specific activity: 66

Km value (mM): 0.26 {methyl-2,3-dehydro-3-hydroxybenzoate}} 0.2 {3-oxocyclohexane-1-carboxylic} 0.3 {2,3-dihydro-2,3-dihydroxybenzoate}} [C]

Substrates: 2,3-Dihydro-2,3-dihydroxybenzoate; Nicotinamide adenine dinucleotide [C]

Specific reaction: 2,3-Dihydro-2,3-dihydroxybenzoate + Nicotinamide adenine dinucleotide <==> 2,3-Dihydroxybenzoate + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]