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The map label for this gene is queA [H]

Identifier: 86361286

GI number: 86361286

Start: 624036

End: 625082

Strand: Reverse

Name: queA [H]

Synonym: RHE_PF00558

Alternate gene names: 86361286

Gene position: 625082-624036 (Counterclockwise)

Preceding gene: 86361287

Following gene: 86361285

Centisome position: 97.29

GC content: 64.37

Gene sequence:

>1047_bases
GTGATCGCCGCTGATCGCCTCGATCGGCAATCGGCCAGGCTGCTCGTCGTGGAGGCGGACGGCAGGATGCGCGACCTGCC
GCGGGCTAACCTCGGGATGTTATTCGATCCTGGCGATCTGGTTGTCGCCAACGATGCCGCAACCTTGCCTGCCAGCCTGC
ACGGCACGCATCTCCGATGTGGAAAGACAATCGAGATCCGCTTGGCCGGCTGGCTGTCAGCTTCCGCTCCAGTCCGCTTC
CTTGCGATCGTCTTCGGCTCTGGTGATCACCGCACCCGCACGGAAGATCGGCTGCCGCCGCCTGCGCTGTCATCAGGCGA
TCGCCTCCAGCTCGGCCCACTTGAAGCCAGGGTCGAGCGCGTTCTCGACCATCCTCGCCTTGTCGAGCTCAGCTTCGAAG
GCACCCGAGCAGCCATGTTTGCAGGACTGGCGCAGCATGGCAGGCCGATTCAATACGCGCATGTTCCCGAGCCGCTGGCA
CTGTGGGATGTTTGGACGAGAATCGCCGCCCGGCCGGTCGCGTTCGAGGCGCCATCGGCTTCCTTCGCGCTCGACTGGCG
CACACTGCAAGCTTGGCGTCGGCGCGAGATCGGTTTTGCAACACTCACGCATGCCGCGGGGATTTCTTCCACCGGCGATC
CCGCGCTCGACTCGCGCCTTCCCTTCGACGAGCCGTACCACATCCCTGAATGTACCGTGGCGCAGGTAGCGCGGGCGCAG
CTCAGGGGCAGCCGCATCATCGCGATCGGCACCAGCGTCGTGCGCGCACTTGAAGCAGCCGCCAATCCCGATGGCAGCGT
GCGTGCCGGAGATGGTATTGCGACAGGGCGCATCGCATGTGGCACTCCGCTCCGCGTCGTCGATGCGGTTCTCACTGGCG
TCCACCAGCCCGGCGAAAGCCATTTCGAGCTGTTGCGCGCCTTCGCCGACGACGGCTTCCTCGCCAGTGCCTCCACCGCT
CTAACTGCATGCGGTTACCGTGCACATGAATTCGGCGATTCCATGCTGCTCAACTGTCAACCGCCACTTTATTGGCGCGA
ACCTTAG

Upstream 100 bases:

>100_bases
GGTCGGCCTTGAAATATCCGAAGCTGGCCGCTGCCGAAATCATCGAGAAGATGCTCGACGCGTTGCCGAGTCACGATGCG
CTTACCGCCGGAGCCCACGC

Downstream 100 bases:

>100_bases
ATGGATAGAGCAGTTTGCATCGCCGTTCCTTGGCGGCCCGCCGGCACTGAAGCGTCGCGTAAGGCCGTTTGGAACATCGG
CGCGCTCGCGCAAGGCGAAC

Product: putative S-adenosylmethionine tRNA ribosyltransferase protein

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MIAADRLDRQSARLLVVEADGRMRDLPRANLGMLFDPGDLVVANDAATLPASLHGTHLRCGKTIEIRLAGWLSASAPVRF
LAIVFGSGDHRTRTEDRLPPPALSSGDRLQLGPLEARVERVLDHPRLVELSFEGTRAAMFAGLAQHGRPIQYAHVPEPLA
LWDVWTRIAARPVAFEAPSASFALDWRTLQAWRRREIGFATLTHAAGISSTGDPALDSRLPFDEPYHIPECTVAQVARAQ
LRGSRIIAIGTSVVRALEAAANPDGSVRAGDGIATGRIACGTPLRVVDAVLTGVHQPGESHFELLRAFADDGFLASASTA
LTACGYRAHEFGDSMLLNCQPPLYWREP

Sequences:

>Translated_348_residues
MIAADRLDRQSARLLVVEADGRMRDLPRANLGMLFDPGDLVVANDAATLPASLHGTHLRCGKTIEIRLAGWLSASAPVRF
LAIVFGSGDHRTRTEDRLPPPALSSGDRLQLGPLEARVERVLDHPRLVELSFEGTRAAMFAGLAQHGRPIQYAHVPEPLA
LWDVWTRIAARPVAFEAPSASFALDWRTLQAWRRREIGFATLTHAAGISSTGDPALDSRLPFDEPYHIPECTVAQVARAQ
LRGSRIIAIGTSVVRALEAAANPDGSVRAGDGIATGRIACGTPLRVVDAVLTGVHQPGESHFELLRAFADDGFLASASTA
LTACGYRAHEFGDSMLLNCQPPLYWREP
>Mature_348_residues
MIAADRLDRQSARLLVVEADGRMRDLPRANLGMLFDPGDLVVANDAATLPASLHGTHLRCGKTIEIRLAGWLSASAPVRF
LAIVFGSGDHRTRTEDRLPPPALSSGDRLQLGPLEARVERVLDHPRLVELSFEGTRAAMFAGLAQHGRPIQYAHVPEPLA
LWDVWTRIAARPVAFEAPSASFALDWRTLQAWRRREIGFATLTHAAGISSTGDPALDSRLPFDEPYHIPECTVAQVARAQ
LRGSRIIAIGTSVVRALEAAANPDGSVRAGDGIATGRIACGTPLRVVDAVLTGVHQPGESHFELLRAFADDGFLASASTA
LTACGYRAHEFGDSMLLNCQPPLYWREP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=348, Percent_Identity=29.0229885057471, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37620; Mature: 37620

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAADRLDRQSARLLVVEADGRMRDLPRANLGMLFDPGDLVVANDAATLPASLHGTHLRC
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCCCEEEC
GKTIEIRLAGWLSASAPVRFLAIVFGSGDHRTRTEDRLPPPALSSGDRLQLGPLEARVER
CCEEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHH
VLDHPRLVELSFEGTRAAMFAGLAQHGRPIQYAHVPEPLALWDVWTRIAARPVAFEAPSA
HHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEECCCC
SFALDWRTLQAWRRREIGFATLTHAAGISSTGDPALDSRLPFDEPYHIPECTVAQVARAQ
CEEEHHHHHHHHHHHCCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LRGSRIIAIGTSVVRALEAAANPDGSVRAGDGIATGRIACGTPLRVVDAVLTGVHQPGES
HCCCEEEEEHHHHHHHHHHHCCCCCCEECCCCCCCCEEECCCHHHHHHHHHHCCCCCCHH
HFELLRAFADDGFLASASTALTACGYRAHEFGDSMLLNCQPPLYWREP
HHHHHHHHHCCCCEEHHHHHHHHCCCHHHHCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MIAADRLDRQSARLLVVEADGRMRDLPRANLGMLFDPGDLVVANDAATLPASLHGTHLRC
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCEEEECCCCCCCCCCCCCEEEC
GKTIEIRLAGWLSASAPVRFLAIVFGSGDHRTRTEDRLPPPALSSGDRLQLGPLEARVER
CCEEEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHH
VLDHPRLVELSFEGTRAAMFAGLAQHGRPIQYAHVPEPLALWDVWTRIAARPVAFEAPSA
HHCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCCEEECCCC
SFALDWRTLQAWRRREIGFATLTHAAGISSTGDPALDSRLPFDEPYHIPECTVAQVARAQ
CEEEHHHHHHHHHHHCCCCEEHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LRGSRIIAIGTSVVRALEAAANPDGSVRAGDGIATGRIACGTPLRVVDAVLTGVHQPGES
HCCCEEEEEHHHHHHHHHHHCCCCCCEECCCCCCCCEEECCCHHHHHHHHHHCCCCCCHH
HFELLRAFADDGFLASASTALTACGYRAHEFGDSMLLNCQPPLYWREP
HHHHHHHHHCCCCEEHHHHHHHHCCCHHHHCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA