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The map label for this gene is yfcX [C]

Identifier: 86361271

GI number: 86361271

Start: 607191

End: 608180

Strand: Direct

Name: yfcX [C]

Synonym: RHE_PF00542

Alternate gene names: 86361271

Gene position: 607191-608180 (Clockwise)

Preceding gene: 86361270

Following gene: 86361272

Centisome position: 94.5

GC content: 61.92

Gene sequence:

>990_bases
ATGAACGTGAGAATGCAGAAATCGACCACATTCGCCACCTTGTCATCGCAGTTTCTCGCCGCTGCGGCGCTGCTGATGCT
GCCGGCGCCCGCACTTGCCGGGCAAACGCTTATCGCGGAAAAATACGACGGCGCGACAGAGATCAGATCTCAACCGGGCA
AAGAGCGGCCGCAGATCAGGATCGACAAGCGGTCGCCCGCCTATTGGCGCGTCACTTTCGACAATCCTCCTTTCAACATC
TTCGGTCCTGAAACCATCCCGCAGATGGAGAAAGTCGTGGCGGAGATCGAGACCGATCCAAATCTGAAGATCGTCGTCTT
CGACAGCGCCGTGCCGGGCTTTTTTCTCACCCACTACAACTTCACCCCGCCGCTTGCAGAATCGACCAGCCTCCCCTCGG
GACGGACGGGTCTCCATCCCCTTCCCGACATGCTGGTGCGCATCAGCAAGGCGCCCGTTGTTTCCGTTGCGCTGATCCGC
GGCCGCGCGACGGGAGTGGGAAGCGAACTTGCGCTTGCGAGCGACATGCGTTTTGCCAGCAGGGAAAAGGCGATCCTGTC
GCAGTGGGAGGTGGGCGCCGGCTTCGTGCCGGGCGGCGGACCGATGGCTCGCCTTCCGCGGCTTGTAGGACGCGGCAGAG
CGCTCGAAGTTTTGCTCGGCTCCGACGATATCAACGGCGAGCTGGCGGAGAAATACGGCTACGTCAACCGCTCCTTTGAC
GACGACAAGCTCGATCCCTTCGTCGACGCCCTCGCCGCGAGGATCTCCGGTTTCGACCGCCAGGCGATCGCAGACACCAA
ATATCTGGTCGATTTCGCCAGCCTGCCGTCGGATCCGGAAATCGGGGCCGGCTGGGATGCCTTCATCTCCTCGGTGCAGC
GCCCCGTGGCGCAGAAGAACATCGGCCGCCTGATGGAGATGGGTTTGCAGACCAACCCCGATATCGAGGCGCGGCTTGGG
CACTACACCCAGACACTGGGCAACGACTAG

Upstream 100 bases:

>100_bases
CGGCGTTTCGAGCGTGCCGCTCGGAATGCCCATCGAGGTCGAGCTCGTCCTCGAGATCGAAACCTAGCGAACTCTTCGCT
CAATTTCCTTGGAGCCAGTC

Downstream 100 bases:

>100_bases
GCCGAAACGCTGCCCCGCGGCGCTACGACCGCGGGCAGCGGCACCGGGCACGTTCGATTGCCCTGCGCATTACCACTGAC
AATTATGGAGACACTACATG

Product: enoyl-CoA hydratase protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNVRMQKSTTFATLSSQFLAAAALLMLPAPALAGQTLIAEKYDGATEIRSQPGKERPQIRIDKRSPAYWRVTFDNPPFNI
FGPETIPQMEKVVAEIETDPNLKIVVFDSAVPGFFLTHYNFTPPLAESTSLPSGRTGLHPLPDMLVRISKAPVVSVALIR
GRATGVGSELALASDMRFASREKAILSQWEVGAGFVPGGGPMARLPRLVGRGRALEVLLGSDDINGELAEKYGYVNRSFD
DDKLDPFVDALAARISGFDRQAIADTKYLVDFASLPSDPEIGAGWDAFISSVQRPVAQKNIGRLMEMGLQTNPDIEARLG
HYTQTLGND

Sequences:

>Translated_329_residues
MNVRMQKSTTFATLSSQFLAAAALLMLPAPALAGQTLIAEKYDGATEIRSQPGKERPQIRIDKRSPAYWRVTFDNPPFNI
FGPETIPQMEKVVAEIETDPNLKIVVFDSAVPGFFLTHYNFTPPLAESTSLPSGRTGLHPLPDMLVRISKAPVVSVALIR
GRATGVGSELALASDMRFASREKAILSQWEVGAGFVPGGGPMARLPRLVGRGRALEVLLGSDDINGELAEKYGYVNRSFD
DDKLDPFVDALAARISGFDRQAIADTKYLVDFASLPSDPEIGAGWDAFISSVQRPVAQKNIGRLMEMGLQTNPDIEARLG
HYTQTLGND
>Mature_329_residues
MNVRMQKSTTFATLSSQFLAAAALLMLPAPALAGQTLIAEKYDGATEIRSQPGKERPQIRIDKRSPAYWRVTFDNPPFNI
FGPETIPQMEKVVAEIETDPNLKIVVFDSAVPGFFLTHYNFTPPLAESTSLPSGRTGLHPLPDMLVRISKAPVVSVALIR
GRATGVGSELALASDMRFASREKAILSQWEVGAGFVPGGGPMARLPRLVGRGRALEVLLGSDDINGELAEKYGYVNRSFD
DDKLDPFVDALAARISGFDRQAIADTKYLVDFASLPSDPEIGAGWDAFISSVQRPVAQKNIGRLMEMGLQTNPDIEARLG
HYTQTLGND

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI1788682, Length=169, Percent_Identity=28.9940828402367, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=175, Percent_Identity=29.7142857142857, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=25.3012048192771, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 35761; Mature: 35761

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRMQKSTTFATLSSQFLAAAALLMLPAPALAGQTLIAEKYDGATEIRSQPGKERPQIR
CCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCCHHHHHCCCCCCCCCEE
IDKRSPAYWRVTFDNPPFNIFGPETIPQMEKVVAEIETDPNLKIVVFDSAVPGFFLTHYN
EECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECC
FTPPLAESTSLPSGRTGLHPLPDMLVRISKAPVVSVALIRGRATGVGSELALASDMRFAS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
REKAILSQWEVGAGFVPGGGPMARLPRLVGRGRALEVLLGSDDINGELAEKYGYVNRSFD
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCCCCCC
DDKLDPFVDALAARISGFDRQAIADTKYLVDFASLPSDPEIGAGWDAFISSVQRPVAQKN
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
IGRLMEMGLQTNPDIEARLGHYTQTLGND
HHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNVRMQKSTTFATLSSQFLAAAALLMLPAPALAGQTLIAEKYDGATEIRSQPGKERPQIR
CCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCCHHHHHCCCCCCCCCEE
IDKRSPAYWRVTFDNPPFNIFGPETIPQMEKVVAEIETDPNLKIVVFDSAVPGFFLTHYN
EECCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEECC
FTPPLAESTSLPSGRTGLHPLPDMLVRISKAPVVSVALIRGRATGVGSELALASDMRFAS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
REKAILSQWEVGAGFVPGGGPMARLPRLVGRGRALEVLLGSDDINGELAEKYGYVNRSFD
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCCCCCC
DDKLDPFVDALAARISGFDRQAIADTKYLVDFASLPSDPEIGAGWDAFISSVQRPVAQKN
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
IGRLMEMGLQTNPDIEARLGHYTQTLGND
HHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA