Click here to switch to the map view.

The map label for this gene is ccmE

Identifier: 86361226

GI number: 86361226

Start: 561827

End: 562294

Strand: Reverse

Name: ccmE

Synonym: RHE_PF00497

Alternate gene names: 86361226

Gene position: 562294-561827 (Counterclockwise)

Preceding gene: 86361227

Following gene: 86361225

Centisome position: 87.51

GC content: 63.03

Gene sequence:

>468_bases
ATGACGCGCAAGCAGAAGCGCCTTGTGGTGATTGCTGGCGGGATGAGTTTCATCCTTGCTGCGGTGCTTTTGGTGATGTT
CGCCTTCAGCCAGTCGGTGGCCTATTTCTACATGCCGGCCGATCTTGCCAGGACGCCGGTGGCGCCGGAGACCCGCATCC
GGCTCGGCGGCCTGGTCGGGGCGGGCAGCGTCGTGCGCGGCGCCGGTTCGACGGTGGAATTTTCTGTCACCGACGGCAGC
GCCAATGCGGTGAAAGTCAAGTATACCGGCATCCTGCCGGATCTGTTCCGCGAGGGCCAGGGTGTGGTCACCGAGGGCAT
GTTTGCCGCAGGCAGCAATGTCTTTATCGCCGACACCGTGCTTGCCAAGCATGACGAGACCTATATGCCGAAGGAGGTGG
CCGACCGACTAAAGTCGCAGGGGCTGTGGCAGGAAGGCAAGGGGCAGGAGGCGAAGGCGACGCCATGA

Upstream 100 bases:

>100_bases
CCTGGCTGTCGCTCGTCAACAAACATATTGCCGCCAATGGCGGGGTGCCGCTGATCGCCGTGGCGCGGGAGCCTCGCATG
ACAGATCAACGAGGTTCGCA

Downstream 100 bases:

>100_bases
TCATCGAGATCGGCCATTACGCTCTGGTGCTGGCGCTGGCGACGGCGCTGATCGTCTCGATCGTGCCTGTCATCGGCGCC
CGCCGTCATGATCCGGCGCT

Product: cytochrome c-type biogenesis protein CcmE

Products: NA

Alternate protein names: Cytochrome c maturation protein E; Heme chaperone CcmE [H]

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MTRKQKRLVVIAGGMSFILAAVLLVMFAFSQSVAYFYMPADLARTPVAPETRIRLGGLVGAGSVVRGAGSTVEFSVTDGS
ANAVKVKYTGILPDLFREGQGVVTEGMFAAGSNVFIADTVLAKHDETYMPKEVADRLKSQGLWQEGKGQEAKATP

Sequences:

>Translated_155_residues
MTRKQKRLVVIAGGMSFILAAVLLVMFAFSQSVAYFYMPADLARTPVAPETRIRLGGLVGAGSVVRGAGSTVEFSVTDGS
ANAVKVKYTGILPDLFREGQGVVTEGMFAAGSNVFIADTVLAKHDETYMPKEVADRLKSQGLWQEGKGQEAKATP
>Mature_154_residues
TRKQKRLVVIAGGMSFILAAVLLVMFAFSQSVAYFYMPADLARTPVAPETRIRLGGLVGAGSVVRGAGSTVEFSVTDGSA
NAVKVKYTGILPDLFREGQGVVTEGMFAAGSNVFIADTVLAKHDETYMPKEVADRLKSQGLWQEGKGQEAKATP

Specific function: Heme chaperone required for the biogenesis of c-type cytochromes. Transiently binds heme delivered by CcmC and transfers the heme to apo-cytochromes in a process facilitated by CcmF and CcmH [H]

COG id: COG2332

COG function: function code O; Cytochrome c-type biogenesis protein CcmE

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmE/CycJ family [H]

Homologues:

Organism=Escherichia coli, GI1788525, Length=145, Percent_Identity=38.6206896551724, Blast_Score=97, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004329
- InterPro:   IPR012340 [H]

Pfam domain/function: PF03100 CcmE [H]

EC number: NA

Molecular weight: Translated: 16488; Mature: 16357

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKQKRLVVIAGGMSFILAAVLLVMFAFSQSVAYFYMPADLARTPVAPETRIRLGGLVG
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCCCCEEEECCEEC
AGSVVRGAGSTVEFSVTDGSANAVKVKYTGILPDLFREGQGVVTEGMFAAGSNVFIADTV
CCCEEECCCCEEEEEEECCCCCEEEEEEECCCHHHHHCCCCCEECCEEECCCCEEEEEHE
LAKHDETYMPKEVADRLKSQGLWQEGKGQEAKATP
ECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRKQKRLVVIAGGMSFILAAVLLVMFAFSQSVAYFYMPADLARTPVAPETRIRLGGLVG
CCCCCEEEEEECCHHHHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCCCCEEEECCEEC
AGSVVRGAGSTVEFSVTDGSANAVKVKYTGILPDLFREGQGVVTEGMFAAGSNVFIADTV
CCCEEECCCCEEEEEEECCCCCEEEEEEECCCHHHHHCCCCCEECCEEECCCCEEEEEHE
LAKHDETYMPKEVADRLKSQGLWQEGKGQEAKATP
ECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10854788 [H]