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The map label for this gene is hemAf

Identifier: 86361219

GI number: 86361219

Start: 552978

End: 554192

Strand: Reverse

Name: hemAf

Synonym: RHE_PF00489

Alternate gene names: 86361219

Gene position: 554192-552978 (Counterclockwise)

Preceding gene: 86361220

Following gene: 86361218

Centisome position: 86.25

GC content: 61.32

Gene sequence:

>1215_bases
ATGGATTTCGAAGCATTTTTCAAAAACGAGCTGGACGGGCTTCATGCCGAGGGCCGCTACCGCGTTTTTGCCGATCTCGA
ACGTCAGCGCGGCCACTTTCCGCGCGCCACGCGCCATACGGCCGATGGTGAAAAGGACGTCACGGTCTGGTGCTCCAACG
ACTATCTCGGAATGGGCCAGAACCCCAAGGTGATCGAGGCGATGAAGGCCGCTATCGACCACTGTGGCGCGGGTGCGGGA
GGCACCCGGAATATTTCTGGCACCAACCACTATCACGTCCTGCTCGAGCGTGAGCTTGCCGATCTGCACGGCAAGGAAGC
AGCCCTGATCTTCACCTCAGGCTATGTCTCCAACTGGGCCTCACTCGGCACGCTCGGCGCAAAAATTCCCGGCCTGATCA
TCTTCTCCGATGCGCTCAACCATGCCTCGATGATCGAGGGCATCCGTCATGCCAAATGCGACAAGGTGATCTGGAAACAT
AATGACGTCGCCGATCTCGAAGCCAAGCTGAAAGCCGCCGATACGAAGGCGCCGAAGCTGATCGCCTTCGAGAGCGTCTA
TTCGATGGACGGCGACATCGCTCCGATCAAGGAGATCTGCGACCTCGCCGACCAATACGGCGCGATGACCTATCTCGACG
AGGTGCATGCGGTCGGCATGTATGGCCCGCGCGGCGGCGGCATTGCCGAGCGCGAGGGGCTGATGGACCGGCTGACGGTC
ATCGAAGGCACGCTCGGCAAGGCCTTCGGAGTGATGGGCGGCTATATCGCCGCGTCGGCAGCGCTGTGCGACTTCATCCG
CTCGTTTGCCTCCGGCTTCATCTTCACCACGGCGCTGCCGCCCGCATTGGCAGCCGGCGCCGTCGCCTCGATCCAGCATC
TGAAGGTCAGTCAGTTCGAGCGCGCCCGCCACCAGGACCGGGTGCGCAAGCTCAGAGCCATGCTCGACCAGCGCGGCATT
CCGCATATGCACAATCCCAGCCATATTGTGCCGGTGATGGTTGGTGATGCGGCCAAATGCAAGTGGATCTCCGATCTGCT
GCTCGACAATTGCGGCGTCTACGTCCAGCCGATCAATTATCCCACCGTACCGAAGAAAACCGAACGGCTGCGCATCACCC
CGACACCGCTGCATTCCGACGCCGATATCGCCCATCTCGTCGACGCCCTGCATTCGCTCTGGTCGCGTTGCGCGCTGGCG
CGAATGGTGGCCTAA

Upstream 100 bases:

>100_bases
CGAAGACCGACGCGGCGAACCACTTGCTCCGAACTTGACGGAGATCAAATACCGTGAGGCTGCTCCCATGTATCCCTTGG
CGGTGCAAAAGGAGCATATG

Downstream 100 bases:

>100_bases
CGAAACGGAAAAGATGCGGGAACTTAATCGCTATAGAAGATTTGCTCTGCGCCCTGGCGATGATGTTCGCGACCGGTCCC
AGCCTGTCGGTTGTCGCTCT

Product: 5-aminolevulinate synthase

Products: NA

Alternate protein names: 5-aminolevulinic acid synthase; Delta-ALA synthase; Delta-aminolevulinate synthase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MDFEAFFKNELDGLHAEGRYRVFADLERQRGHFPRATRHTADGEKDVTVWCSNDYLGMGQNPKVIEAMKAAIDHCGAGAG
GTRNISGTNHYHVLLERELADLHGKEAALIFTSGYVSNWASLGTLGAKIPGLIIFSDALNHASMIEGIRHAKCDKVIWKH
NDVADLEAKLKAADTKAPKLIAFESVYSMDGDIAPIKEICDLADQYGAMTYLDEVHAVGMYGPRGGGIAEREGLMDRLTV
IEGTLGKAFGVMGGYIAASAALCDFIRSFASGFIFTTALPPALAAGAVASIQHLKVSQFERARHQDRVRKLRAMLDQRGI
PHMHNPSHIVPVMVGDAAKCKWISDLLLDNCGVYVQPINYPTVPKKTERLRITPTPLHSDADIAHLVDALHSLWSRCALA
RMVA

Sequences:

>Translated_404_residues
MDFEAFFKNELDGLHAEGRYRVFADLERQRGHFPRATRHTADGEKDVTVWCSNDYLGMGQNPKVIEAMKAAIDHCGAGAG
GTRNISGTNHYHVLLERELADLHGKEAALIFTSGYVSNWASLGTLGAKIPGLIIFSDALNHASMIEGIRHAKCDKVIWKH
NDVADLEAKLKAADTKAPKLIAFESVYSMDGDIAPIKEICDLADQYGAMTYLDEVHAVGMYGPRGGGIAEREGLMDRLTV
IEGTLGKAFGVMGGYIAASAALCDFIRSFASGFIFTTALPPALAAGAVASIQHLKVSQFERARHQDRVRKLRAMLDQRGI
PHMHNPSHIVPVMVGDAAKCKWISDLLLDNCGVYVQPINYPTVPKKTERLRITPTPLHSDADIAHLVDALHSLWSRCALA
RMVA
>Mature_404_residues
MDFEAFFKNELDGLHAEGRYRVFADLERQRGHFPRATRHTADGEKDVTVWCSNDYLGMGQNPKVIEAMKAAIDHCGAGAG
GTRNISGTNHYHVLLERELADLHGKEAALIFTSGYVSNWASLGTLGAKIPGLIIFSDALNHASMIEGIRHAKCDKVIWKH
NDVADLEAKLKAADTKAPKLIAFESVYSMDGDIAPIKEICDLADQYGAMTYLDEVHAVGMYGPRGGGIAEREGLMDRLTV
IEGTLGKAFGVMGGYIAASAALCDFIRSFASGFIFTTALPPALAAGAVASIQHLKVSQFERARHQDRVRKLRAMLDQRGI
PHMHNPSHIVPVMVGDAAKCKWISDLLLDNCGVYVQPINYPTVPKKTERLRITPTPLHSDADIAHLVDALHSLWSRCALA
RMVA

Specific function: Unknown

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI83977442, Length=403, Percent_Identity=48.1389578163772, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI83977440, Length=403, Percent_Identity=47.8908188585608, Blast_Score=399, Evalue=1e-111,
Organism=Homo sapiens, GI83977444, Length=403, Percent_Identity=48.1389578163772, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI4502025, Length=403, Percent_Identity=49.3796526054591, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI40316939, Length=403, Percent_Identity=49.3796526054591, Blast_Score=395, Evalue=1e-110,
Organism=Homo sapiens, GI7657118, Length=391, Percent_Identity=35.5498721227622, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI284448556, Length=342, Percent_Identity=38.3040935672515, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI4758668, Length=404, Percent_Identity=30.6930693069307, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI119220554, Length=391, Percent_Identity=28.3887468030691, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI5454084, Length=259, Percent_Identity=28.957528957529, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1790046, Length=393, Percent_Identity=35.6234096692112, Blast_Score=217, Evalue=8e-58,
Organism=Escherichia coli, GI1786993, Length=344, Percent_Identity=33.1395348837209, Blast_Score=165, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI71994529, Length=397, Percent_Identity=34.2569269521411, Blast_Score=230, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI32566772, Length=376, Percent_Identity=29.2553191489362, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17560914, Length=368, Percent_Identity=27.7173913043478, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17560912, Length=368, Percent_Identity=27.7173913043478, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71982617, Length=263, Percent_Identity=26.2357414448669, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982625, Length=263, Percent_Identity=26.2357414448669, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320438, Length=425, Percent_Identity=48.4705882352941, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6320267, Length=354, Percent_Identity=27.1186440677966, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6323954, Length=293, Percent_Identity=23.2081911262799, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI17137420, Length=405, Percent_Identity=46.9135802469136, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24662918, Length=358, Percent_Identity=35.4748603351955, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI17136286, Length=330, Percent_Identity=27.8787878787879, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24653280, Length=261, Percent_Identity=28.735632183908, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653276, Length=261, Percent_Identity=28.735632183908, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653278, Length=261, Percent_Identity=28.735632183908, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010961
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.37 [H]

Molecular weight: Translated: 44125; Mature: 44125

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFEAFFKNELDGLHAEGRYRVFADLERQRGHFPRATRHTADGEKDVTVWCSNDYLGMGQ
CCHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
NPKVIEAMKAAIDHCGAGAGGTRNISGTNHYHVLLERELADLHGKEAALIFTSGYVSNWA
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCEEEEEECCHHHHHH
SLGTLGAKIPGLIIFSDALNHASMIEGIRHAKCDKVIWKHNDVADLEAKLKAADTKAPKL
HHHHHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCE
IAFESVYSMDGDIAPIKEICDLADQYGAMTYLDEVHAVGMYGPRGGGIAEREGLMDRLTV
EEEHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IEGTLGKAFGVMGGYIAASAALCDFIRSFASGFIFTTALPPALAAGAVASIQHLKVSQFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
RARHQDRVRKLRAMLDQRGIPHMHNPSHIVPVMVGDAAKCKWISDLLLDNCGVYVQPINY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEECCC
PTVPKKTERLRITPTPLHSDADIAHLVDALHSLWSRCALARMVA
CCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFEAFFKNELDGLHAEGRYRVFADLERQRGHFPRATRHTADGEKDVTVWCSNDYLGMGQ
CCHHHHHHHHHCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCC
NPKVIEAMKAAIDHCGAGAGGTRNISGTNHYHVLLERELADLHGKEAALIFTSGYVSNWA
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCEEEEEECCHHHHHH
SLGTLGAKIPGLIIFSDALNHASMIEGIRHAKCDKVIWKHNDVADLEAKLKAADTKAPKL
HHHHHHHCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCE
IAFESVYSMDGDIAPIKEICDLADQYGAMTYLDEVHAVGMYGPRGGGIAEREGLMDRLTV
EEEHHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IEGTLGKAFGVMGGYIAASAALCDFIRSFASGFIFTTALPPALAAGAVASIQHLKVSQFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHH
RARHQDRVRKLRAMLDQRGIPHMHNPSHIVPVMVGDAAKCKWISDLLLDNCGVYVQPINY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEECCC
PTVPKKTERLRITPTPLHSDADIAHLVDALHSLWSRCALARMVA
CCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034217 [H]