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The map label for this gene is vbsL

Identifier: 86361191

GI number: 86361191

Start: 520689

End: 521876

Strand: Direct

Name: vbsL

Synonym: RHE_PF00461

Alternate gene names: 86361191

Gene position: 520689-521876 (Clockwise)

Preceding gene: 86361190

Following gene: 86361193

Centisome position: 81.04

GC content: 64.65

Gene sequence:

>1188_bases
ATGAACCAGTCCTTCAGCCCCCGCGACCTTGGCGCCCATGCGGCCGCCGACGATCTCTTCACCCCCGTCCATCCGGCCGC
CTTCAACGCCGTCTCGCCGCCGATCTTCCAGACCTCGCTCTTTACCTATGATAGCTACGAGGCGATGGAGGATGTCTTCG
CCGGCCGGACGCGCAACTTCATCTATTCGCGCGGTGACAATCCGACCGTTCGCGAATTCGAACTGCTGGTCGCCCGCCTC
GAAGGCGCGGAGGATGGGCGCGCCTTTTCCAGCGGAACGGCCGCCATCACCTCGACCATTCTCAGCCTGGTGGAAGCGGG
CGACCGCGTCGTTGCCGTCCGCCATCTCTATAATGACGTCTATCGCCTTCTGGTGAAGCTGCTCGGCCGGCTCGGCGTCA
CCGTCGATTTTGTCGATCCCTCCGACCATGACGAGGTGCGCAAGGCGCTGCCGGGCGCCAAGCTGCTCTATCTCGAAAAC
CCGTCTTCCTTCATCTTCGAGCTTCAGGACATCGTCGCGCTGTCGGCCATGGCGAAAGAGGCCGGCGTGACGACCATCAT
CGACAATAGCTGGGCGACGCCGCTTTTCCAGAAGCCGGTCCAGCATGGCGTCGATATCGTCGTCCACGCCGCCTCGAAAT
ATCTCGGCGGCCATAGCGATACGGTCGCCGGCGTCGTCGTCGGCTCGAAGGAGGCGATCGCGAAGATCAACTCGACATCC
TACCCTTATGTCGGCGCCAAGCTCGCGCCCTTCGAGGCCTGGCTGCTGCTGCGCGGCATGCGCACGCTGCGGGTGCGCCT
CAAGGAGCACGAGCGCAGCGGGCTTCTGCTCGCCGATCGGCTGAAGCAGCATCCCGGCATCGCGCGTGTCCGCCACCCGG
CCTTTCAGGACCATCCCGGGCGGGCGACGCTTACGGGTTATGCCGGGCTCTTCGCCTTCGACCTCACGCCCGATATCGAT
GTCGCGCGCTTCGTCAACGCGCTTCGCGAGATCCGCCTCGGCGTCAGCTGGGGAGGGCCGGAAACGCTGGTGGTTCCGGC
CAAGGTCGCGCTGCAGATCCCCGACCGGATGACCACCTTCATCCGGTTCGGCGTGAGCGAGCAGACGATCCGTTTCGCTG
TTGGGCTGGAAGAGCCGGAATTGCTGTGGAACGATTTGCAGCAGGCGCTGCACGCCGCGAAGCGGTGA

Upstream 100 bases:

>100_bases
TTCTGCCTCGCCCGATCCCTCGGCGCGCTCCGGCAGCGCTCGGACGAACCTCTGACATCAATCCGTCCCTATCCCCGCTT
TTGTCCATGAGAGGATTATC

Downstream 100 bases:

>100_bases
TTTCCCGCGGGCGCTCGTGATCGGGGCGGGCAGGCTCGGTCATTGCCAGATGGCAATGCTGCTCCGCCGCCTTGATCATG
AAATTGACGAGCGTTCGGGA

Product: hypothetical protein

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNQSFSPRDLGAHAAADDLFTPVHPAAFNAVSPPIFQTSLFTYDSYEAMEDVFAGRTRNFIYSRGDNPTVREFELLVARL
EGAEDGRAFSSGTAAITSTILSLVEAGDRVVAVRHLYNDVYRLLVKLLGRLGVTVDFVDPSDHDEVRKALPGAKLLYLEN
PSSFIFELQDIVALSAMAKEAGVTTIIDNSWATPLFQKPVQHGVDIVVHAASKYLGGHSDTVAGVVVGSKEAIAKINSTS
YPYVGAKLAPFEAWLLLRGMRTLRVRLKEHERSGLLLADRLKQHPGIARVRHPAFQDHPGRATLTGYAGLFAFDLTPDID
VARFVNALREIRLGVSWGGPETLVVPAKVALQIPDRMTTFIRFGVSEQTIRFAVGLEEPELLWNDLQQALHAAKR

Sequences:

>Translated_395_residues
MNQSFSPRDLGAHAAADDLFTPVHPAAFNAVSPPIFQTSLFTYDSYEAMEDVFAGRTRNFIYSRGDNPTVREFELLVARL
EGAEDGRAFSSGTAAITSTILSLVEAGDRVVAVRHLYNDVYRLLVKLLGRLGVTVDFVDPSDHDEVRKALPGAKLLYLEN
PSSFIFELQDIVALSAMAKEAGVTTIIDNSWATPLFQKPVQHGVDIVVHAASKYLGGHSDTVAGVVVGSKEAIAKINSTS
YPYVGAKLAPFEAWLLLRGMRTLRVRLKEHERSGLLLADRLKQHPGIARVRHPAFQDHPGRATLTGYAGLFAFDLTPDID
VARFVNALREIRLGVSWGGPETLVVPAKVALQIPDRMTTFIRFGVSEQTIRFAVGLEEPELLWNDLQQALHAAKR
>Mature_395_residues
MNQSFSPRDLGAHAAADDLFTPVHPAAFNAVSPPIFQTSLFTYDSYEAMEDVFAGRTRNFIYSRGDNPTVREFELLVARL
EGAEDGRAFSSGTAAITSTILSLVEAGDRVVAVRHLYNDVYRLLVKLLGRLGVTVDFVDPSDHDEVRKALPGAKLLYLEN
PSSFIFELQDIVALSAMAKEAGVTTIIDNSWATPLFQKPVQHGVDIVVHAASKYLGGHSDTVAGVVVGSKEAIAKINSTS
YPYVGAKLAPFEAWLLLRGMRTLRVRLKEHERSGLLLADRLKQHPGIARVRHPAFQDHPGRATLTGYAGLFAFDLTPDID
VARFVNALREIRLGVSWGGPETLVVPAKVALQIPDRMTTFIRFGVSEQTIRFAVGLEEPELLWNDLQQALHAAKR

Specific function: Methionine biosynthesis; third step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=378, Percent_Identity=32.010582010582, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI299473759, Length=378, Percent_Identity=28.5714285714286, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI25453487, Length=376, Percent_Identity=30.3191489361702, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1789383, Length=373, Percent_Identity=31.3672922252011, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1790375, Length=374, Percent_Identity=29.6791443850267, Blast_Score=170, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17560056, Length=382, Percent_Identity=32.1989528795811, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=382, Percent_Identity=32.7225130890052, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI71980708, Length=373, Percent_Identity=28.9544235924933, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6321170, Length=317, Percent_Identity=35.3312302839117, Blast_Score=182, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6319307, Length=350, Percent_Identity=31.7142857142857, Blast_Score=153, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6323333, Length=410, Percent_Identity=25.609756097561, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321254, Length=317, Percent_Identity=29.3375394321767, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=374, Percent_Identity=25.9358288770053, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=30.1587301587302, Blast_Score=192, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 43385; Mature: 43385

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQSFSPRDLGAHAAADDLFTPVHPAAFNAVSPPIFQTSLFTYDSYEAMEDVFAGRTRNF
CCCCCCCCCCCCHHHHHHCCCCCCCHHHCCCCCCHHHHEEECCCCHHHHHHHHCCCCCCE
IYSRGDNPTVREFELLVARLEGAEDGRAFSSGTAAITSTILSLVEAGDRVVAVRHLYNDV
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHH
YRLLVKLLGRLGVTVDFVDPSDHDEVRKALPGAKLLYLENPSSFIFELQDIVALSAMAKE
HHHHHHHHHHHCCEEEECCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
AGVTTIIDNSWATPLFQKPVQHGVDIVVHAASKYLGGHSDTVAGVVVGSKEAIAKINSTS
CCCEEEECCCCCCHHHHHHHHCCCEEEEEHHHHHCCCCCCCEEEEEECCHHHHHHCCCCC
YPYVGAKLAPFEAWLLLRGMRTLRVRLKEHERSGLLLADRLKQHPGIARVRHPAFQDHPG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHCCCCCCCCCC
RATLTGYAGLFAFDLTPDIDVARFVNALREIRLGVSWGGPETLVVPAKVALQIPDRMTTF
CEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEECCHHHHHH
IRFGVSEQTIRFAVGLEEPELLWNDLQQALHAAKR
HHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNQSFSPRDLGAHAAADDLFTPVHPAAFNAVSPPIFQTSLFTYDSYEAMEDVFAGRTRNF
CCCCCCCCCCCCHHHHHHCCCCCCCHHHCCCCCCHHHHEEECCCCHHHHHHHHCCCCCCE
IYSRGDNPTVREFELLVARLEGAEDGRAFSSGTAAITSTILSLVEAGDRVVAVRHLYNDV
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEHHHHHHHHH
YRLLVKLLGRLGVTVDFVDPSDHDEVRKALPGAKLLYLENPSSFIFELQDIVALSAMAKE
HHHHHHHHHHHCCEEEECCCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
AGVTTIIDNSWATPLFQKPVQHGVDIVVHAASKYLGGHSDTVAGVVVGSKEAIAKINSTS
CCCEEEECCCCCCHHHHHHHHCCCEEEEEHHHHHCCCCCCCEEEEEECCHHHHHHCCCCC
YPYVGAKLAPFEAWLLLRGMRTLRVRLKEHERSGLLLADRLKQHPGIARVRHPAFQDHPG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHCCCCCCCCCC
RATLTGYAGLFAFDLTPDIDVARFVNALREIRLGVSWGGPETLVVPAKVALQIPDRMTTF
CEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEECCHHHHHH
IRFGVSEQTIRFAVGLEEPELLWNDLQQALHAAKR
HHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]