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The map label for this gene is raiI

Identifier: 86361170

GI number: 86361170

Start: 492770

End: 493408

Strand: Reverse

Name: raiI

Synonym: RHE_PF00440

Alternate gene names: 86361170

Gene position: 493408-492770 (Counterclockwise)

Preceding gene: 86361180

Following gene: 86361169

Centisome position: 76.79

GC content: 60.25

Gene sequence:

>639_bases
ATGTTGCGGATACTCACCAAAGACATGCTCGAGACCGATCGACGTGCTTTCGATGAAATGTTTCGGGCTCGCGCCGCCGT
TTTTCGCGATCGGCTGGGATGGCAGGTCGATGTCCGTGATCAATGGGAGAGGGACCGATATGACGAGGCCGAAGATCCCG
TCTATCTCGTCACGCAACGACCTTCCGGCACGCTGACGGGTTCGCTGCGCCTGCTGCCGACCACCGGAGCGACGATGCTC
AAAAGCGAGTTCCGGCATTTCTTCGATCAGCCTATCGACGTCGATAGCCCGACGACCTGGGAATGTACCCGCTTTTGCCT
TCATCCGCATGCCGGACACATGGAGCAATCGCGCGCAGTCGCCACGGAGCTGCTCTCCGGGCTTTGCGATCTTGCCCTCG
ACACCGGTATCGAGAGCATTGTCGGCGTCTATGACGCCGCGATGGTTGCTGTGTACCGAAGGATCGGCTGGAGGCCGATG
CCGCTTGCCCGATCCCGGCCCGAGATCGGCAAGCTGTATGTTGGCCTATGGGATGTGACGGCGGACAATTGTCGGACACT
GCGGGCCAACCTGTACCGGCTTCTCGAGCAAGCCTCTCCCAATCCTGCCAGAGCCCCTGTCAATGGTGGCATGGGATAG

Upstream 100 bases:

>100_bases
GGGGAAGGCAAAGCCAAGCACCGGTCAACCTGGTGAAATATCCAGATTGCTCCGGCTCGGCTTCCTGTTGTGAAACGTCA
GCACATCAGGAGGGACGGAT

Downstream 100 bases:

>100_bases
GCTCAGCGCGGTCCGCCGCCCAGCATAGGATGGATCTCGCGAGTTCTTTGACCTGTTCTTCATGTCGCGTCATGGTTGCC
ACAAAAAGCTGCTGTTCTCG

Product: autoinducer synthesis protein

Products: NA

Alternate protein names: Autoinducer synthesis protein raiI [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLRILTKDMLETDRRAFDEMFRARAAVFRDRLGWQVDVRDQWERDRYDEAEDPVYLVTQRPSGTLTGSLRLLPTTGATML
KSEFRHFFDQPIDVDSPTTWECTRFCLHPHAGHMEQSRAVATELLSGLCDLALDTGIESIVGVYDAAMVAVYRRIGWRPM
PLARSRPEIGKLYVGLWDVTADNCRTLRANLYRLLEQASPNPARAPVNGGMG

Sequences:

>Translated_212_residues
MLRILTKDMLETDRRAFDEMFRARAAVFRDRLGWQVDVRDQWERDRYDEAEDPVYLVTQRPSGTLTGSLRLLPTTGATML
KSEFRHFFDQPIDVDSPTTWECTRFCLHPHAGHMEQSRAVATELLSGLCDLALDTGIESIVGVYDAAMVAVYRRIGWRPM
PLARSRPEIGKLYVGLWDVTADNCRTLRANLYRLLEQASPNPARAPVNGGMG
>Mature_212_residues
MLRILTKDMLETDRRAFDEMFRARAAVFRDRLGWQVDVRDQWERDRYDEAEDPVYLVTQRPSGTLTGSLRLLPTTGATML
KSEFRHFFDQPIDVDSPTTWECTRFCLHPHAGHMEQSRAVATELLSGLCDLALDTGIESIVGVYDAAMVAVYRRIGWRPM
PLARSRPEIGKLYVGLWDVTADNCRTLRANLYRLLEQASPNPARAPVNGGMG

Specific function: Required for the synthesis of autoinducer molecules which bind to raiR and that are involved in the restriction of nodule number [H]

COG id: COG3916

COG function: function code TQ; N-acyl-L-homoserine lactone synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the autoinducer synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR001690
- InterPro:   IPR018311 [H]

Pfam domain/function: PF00765 Autoind_synth [H]

EC number: =2.3.1.184 [H]

Molecular weight: Translated: 24118; Mature: 24118

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00949 AUTOINDUCERS_SYNTH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRILTKDMLETDRRAFDEMFRARAAVFRDRLGWQVDVRDQWERDRYDEAEDPVYLVTQR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHCCCCCCCCEEEEEEC
PSGTLTGSLRLLPTTGATMLKSEFRHFFDQPIDVDSPTTWECTRFCLHPHAGHMEQSRAV
CCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHH
ATELLSGLCDLALDTGIESIVGVYDAAMVAVYRRIGWRPMPLARSRPEIGKLYVGLWDVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEECC
ADNCRTLRANLYRLLEQASPNPARAPVNGGMG
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRILTKDMLETDRRAFDEMFRARAAVFRDRLGWQVDVRDQWERDRYDEAEDPVYLVTQR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHCCCCCCCCEEEEEEC
PSGTLTGSLRLLPTTGATMLKSEFRHFFDQPIDVDSPTTWECTRFCLHPHAGHMEQSRAV
CCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHH
ATELLSGLCDLALDTGIESIVGVYDAAMVAVYRRIGWRPMPLARSRPEIGKLYVGLWDVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEECC
ADNCRTLRANLYRLLEQASPNPARAPVNGGMG
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9473034 [H]