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The map label for this gene is proW [H]

Identifier: 86361141

GI number: 86361141

Start: 463894

End: 464781

Strand: Direct

Name: proW [H]

Synonym: RHE_PF00411

Alternate gene names: 86361141

Gene position: 463894-464781 (Clockwise)

Preceding gene: 86361140

Following gene: 86361142

Centisome position: 72.2

GC content: 58.9

Gene sequence:

>888_bases
ATGGAATGGTTTTATAAATTCCCGCACATGAACGACGACGCGCTTCGCAACCTGAAGAAAGCGATCGATGACGGCTTTCG
CGGATTTACCCGCAGCTATGGCGACGGCATCGAAAGCCTCTTCGTCCCGCTGCAGCACTTCCTCATAGCTGCCGAGCGAT
TCATGACGCAAACGCCGTGGCCGATCATTACCCTGATCATCCTGGTCATTGCCTGGTTTGCGAGCCGCAGCTTGAAGATC
GTCGCCGGCTGTCTGGTGACTCTGATGCTGATCGGCTATTTCGACATGTGGGACGATACGATGCGGACGGTCTCGATGAT
CTTCGTCTGCACGGTGCTTTCCATCGCTATCGGCATTCCGATCGGGATCCTGATGGCGCGCTCCGACCGGCTGCAGCGCG
TCGTCAATCCGATCCTCGACGTGATGCAGACGATGCCGAGCTTCGTCTATCTCATCCCCGTCGTCATGCTGCTCGGCATC
GGCAAGGTGCCCGGTCTGATCGCCGTCGTCATCTACGCGATCCCGCCGATGATCCGGCTCACCGATCTCGGCATCCGACT
CGTCGACAAGGACGTGCTCGAGGCCGCAGATGCCTTCGGCACCTCCAGAGCGCAGAAGCTTTTCAAGGTGCAGCTGCCAC
TGGCGCTTCCCACCATCATGGCCGGCATCAACCAGACGATCATGATGGCGCTCGCCATGGTGGTCATCGCTTCCATGATC
GGCGTCCAGGGCCTCGGCCAGCCGGTACTCAAGGCAATCGCCAACCAGTACTTCACGCTGGGCATTTTCAACGGCCTTGC
CATTGTCGGCATCGCCATCATCTTCGACCGGGTCAGCCAGGCATATGGCAAAAGGCTCCAGAAGCATCGGGAGACTGTCC
ATGGCTGA

Upstream 100 bases:

>100_bases
CTCTTTGAGCCAAAATTGCGGTGCGCCAAAAGCGCACCGCTTCTCACGCGCCGACAAAATCAAAAGACTGCCACGGCTGT
TTGACGAAAGGGGAACCGAG

Downstream 100 bases:

>100_bases
GAATCATTTCGGCGGTATCAAGATCCGCAACCTCTACAAGATCTTTGGCCCCAACCCCGCCGCTCATGTCGATGCGGTTC
AGAAGGGCCTGACGAAGACC

Product: amino acid ABC transporter permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MEWFYKFPHMNDDALRNLKKAIDDGFRGFTRSYGDGIESLFVPLQHFLIAAERFMTQTPWPIITLIILVIAWFASRSLKI
VAGCLVTLMLIGYFDMWDDTMRTVSMIFVCTVLSIAIGIPIGILMARSDRLQRVVNPILDVMQTMPSFVYLIPVVMLLGI
GKVPGLIAVVIYAIPPMIRLTDLGIRLVDKDVLEAADAFGTSRAQKLFKVQLPLALPTIMAGINQTIMMALAMVVIASMI
GVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQAYGKRLQKHRETVHG

Sequences:

>Translated_295_residues
MEWFYKFPHMNDDALRNLKKAIDDGFRGFTRSYGDGIESLFVPLQHFLIAAERFMTQTPWPIITLIILVIAWFASRSLKI
VAGCLVTLMLIGYFDMWDDTMRTVSMIFVCTVLSIAIGIPIGILMARSDRLQRVVNPILDVMQTMPSFVYLIPVVMLLGI
GKVPGLIAVVIYAIPPMIRLTDLGIRLVDKDVLEAADAFGTSRAQKLFKVQLPLALPTIMAGINQTIMMALAMVVIASMI
GVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQAYGKRLQKHRETVHG
>Mature_295_residues
MEWFYKFPHMNDDALRNLKKAIDDGFRGFTRSYGDGIESLFVPLQHFLIAAERFMTQTPWPIITLIILVIAWFASRSLKI
VAGCLVTLMLIGYFDMWDDTMRTVSMIFVCTVLSIAIGIPIGILMARSDRLQRVVNPILDVMQTMPSFVYLIPVVMLLGI
GKVPGLIAVVIYAIPPMIRLTDLGIRLVDKDVLEAADAFGTSRAQKLFKVQLPLALPTIMAGINQTIMMALAMVVIASMI
GVQGLGQPVLKAIANQYFTLGIFNGLAIVGIAIIFDRVSQAYGKRLQKHRETVHG

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=302, Percent_Identity=41.3907284768212, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1788451, Length=199, Percent_Identity=31.6582914572864, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788449, Length=189, Percent_Identity=30.1587301587302, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1786564, Length=198, Percent_Identity=26.7676767676768, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32731; Mature: 32731

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2649479; 9205837; 9278503 [H]