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The map label for this gene is stcD

Identifier: 86361137

GI number: 86361137

Start: 459114

End: 461150

Strand: Reverse

Name: stcD

Synonym: RHE_PF00407

Alternate gene names: 86361137

Gene position: 461150-459114 (Counterclockwise)

Preceding gene: 86361143

Following gene: 86361136

Centisome position: 71.77

GC content: 61.02

Gene sequence:

>2037_bases
ATGTCGAACGATCCTCTTCTTCAGCCTTACCAGCTCAAGCATCTGACGCTGCGTAACCGTATCATCGTAACCTCGCACGA
GCCGGCCTATCCCGAGGACGGCATGCCGAAGGAGAGGTATCGGGCCTATACGGTGGAGCGGGCAAAAGGCGGGGTGGCCT
TGACGATGACGGCAGGCTCAGCCGCGGTTTCCAGGGATAGTCCGCCTGTTTTCAACAACCTGCTGGCCTATAAGGACGAG
ATCGTACCCTGGATCAGGGAAATGACCGACGCCGTCCACGAACAGGGTGCGGCGATCATGATCCAGCTCACCCATCTCGG
CCGGCGCACGCGCTGGGACAAGGGTGACTGGCTGCCGGTCGTCGCCCCGTCGCATCATCGCGAGGCTTCGCATCGCGCCT
TCCCGAAGAAGATGGAAGATTGGGACATCGAGCGCATCATCAAGGATTTCGCCGATGCGGCCGAACGGATGAAGGCGGGC
GGTATGGATGGAATCGAGCTGGAGGCCTACGGCCACCTGATCGACCAGTTCGCATCTCCGCTCACGAACGAACTCGGTGG
CCCCTACGGGGGTGCGCTCGAGAACCGCATGCGCTTCTGTTTCGATGTGTTCAGGGCGATCCGCGAGAGGGTCGGGAACG
AATTCATCCTCGGCGTGCGCTATACGGCGGACGAATGTCTGCCCGGCGGTAACGGACAGGCCGAAGGCATAGAGATTTCG
AAGCGGCTGCGCGACAGCGGGCTTATCGATTATCTGAACGTCATTCGCGGCCACATCGACACCGACCCGGGGCTGACCGA
CGTCATCCCGATCCAGGGCATGGCGAACTCACCGCATCTGGATTTTGCCGGTGAAATCCGCGCAGCGACCAACTTCCCGA
CCTTCCATGCGGCGAAGATTCCCGACGTCGCCACCGCGCGTCATGCGATCGCAGCCGGCAAGGTCGACATGGTCGGCATG
ACCCGCGCCCACATGACTGACCCGCACATCGTGCGCAAGATTATCGAGAAGAGGGAGGAGGATATCCGCCCCTGCGTCGG
CGCCAACTACTGTCTCGACCGCATCTATCAGGGCGGGGCCGCCTACTGCATCCATAATGCCGCCACCGGCCGCGAACTGA
CGATGCCGCATATCGTGGCAAAGGCCGACGTCGGAAAGAAGGTCGTCATCGTCGGCGCCGGCCCGGCCGGTCTGGAAGCG
GCGCGCGTCGCGGGAGAGCGTGGTCACGAAGTGGTCGTTTTCGAAGCTGCCAACAATCCAGGGGGGCAGATCCGTCTCAC
CGCCCAAAGTGAACGTCGTCGGGAAATGATCAGCATCATCGACTGGCGCATGAGGCAGTGTGAGAAATACGATGTCACCT
TCCACTTCAACACCTGGGCGGAAGCCGATACGATCGAGGCCGAAAATCCTGACGTCGTCATCATCGCCACAGGCGGCTTG
CCGCATACCGAGGTGCTCACCAACGGCAACGAGCTGGTGGTCTCCTCTTGGGACATCATCTCCGGCGATGTGAAGCCCGG
AACCAATGTGCTCGTTTTCGACGATGCCGGCGACCATGCCGGTCTCCAGGCGGCGGAGTTCCTCGCCAACGCAGGCGCCA
AGGTCGAGATCATGACGCCGGACCGGTCCTTCGCGCCGGAGGTCATGGCCATGAACCTGGTGCCCTATATGCGTTCGCTC
CAAAAGTACGACGTGACCTTCACCGTTACCTATCGGCTGGAAGCCGTCGAGAAGAGCGGTAACCAGCTCGTCGCCCTTAT
CGGCAGCGATTACGGCGGAATTGCCCGGCAGCATAGCTACGACCAGGTCGTCATCAATCACGGGACCATCCCGCTCGACG
ACCTCTATTTCGAGCTGAAGCCCAAGTCGAAAAATCTCGGCGAGGTCTCGTACGACCAGCTTCTCCAGGGGCAACCGCAA
TCAGTCATCCACAATCCCGAGGGCAAGTTTCAGCTGTTCCGGATCGGTGACGCTGTCGCTGCCCGCAACACGCATGCCGC
GGTCTATGACGGTCTGCGCATCGCAAAGGATATATGA

Upstream 100 bases:

>100_bases
CATTAGTGTCTATAACAATGACCTTCATGTTTTCAATGTTGACACATATGTACATTGCCCTTAGCGTTTGCTTCGATGTT
TTAATCCGGAACCATGGCCC

Downstream 100 bases:

>100_bases
TCGCAAGAGAGGCCGCCTGTCGGCCGATCGGGAGGGAATGATATGAGTTTGCGCAACGGAAGCCTCCAGCATTTCCTGGA
AGCCGCTTCGGTCGCTTTCG

Product: 2,4-dienoyl-CoA reductase (NADPH) protein

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MSNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKERYRAYTVERAKGGVALTMTAGSAAVSRDSPPVFNNLLAYKDE
IVPWIREMTDAVHEQGAAIMIQLTHLGRRTRWDKGDWLPVVAPSHHREASHRAFPKKMEDWDIERIIKDFADAAERMKAG
GMDGIELEAYGHLIDQFASPLTNELGGPYGGALENRMRFCFDVFRAIRERVGNEFILGVRYTADECLPGGNGQAEGIEIS
KRLRDSGLIDYLNVIRGHIDTDPGLTDVIPIQGMANSPHLDFAGEIRAATNFPTFHAAKIPDVATARHAIAAGKVDMVGM
TRAHMTDPHIVRKIIEKREEDIRPCVGANYCLDRIYQGGAAYCIHNAATGRELTMPHIVAKADVGKKVVIVGAGPAGLEA
ARVAGERGHEVVVFEAANNPGGQIRLTAQSERRREMISIIDWRMRQCEKYDVTFHFNTWAEADTIEAENPDVVIIATGGL
PHTEVLTNGNELVVSSWDIISGDVKPGTNVLVFDDAGDHAGLQAAEFLANAGAKVEIMTPDRSFAPEVMAMNLVPYMRSL
QKYDVTFTVTYRLEAVEKSGNQLVALIGSDYGGIARQHSYDQVVINHGTIPLDDLYFELKPKSKNLGEVSYDQLLQGQPQ
SVIHNPEGKFQLFRIGDAVAARNTHAAVYDGLRIAKDI

Sequences:

>Translated_678_residues
MSNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKERYRAYTVERAKGGVALTMTAGSAAVSRDSPPVFNNLLAYKDE
IVPWIREMTDAVHEQGAAIMIQLTHLGRRTRWDKGDWLPVVAPSHHREASHRAFPKKMEDWDIERIIKDFADAAERMKAG
GMDGIELEAYGHLIDQFASPLTNELGGPYGGALENRMRFCFDVFRAIRERVGNEFILGVRYTADECLPGGNGQAEGIEIS
KRLRDSGLIDYLNVIRGHIDTDPGLTDVIPIQGMANSPHLDFAGEIRAATNFPTFHAAKIPDVATARHAIAAGKVDMVGM
TRAHMTDPHIVRKIIEKREEDIRPCVGANYCLDRIYQGGAAYCIHNAATGRELTMPHIVAKADVGKKVVIVGAGPAGLEA
ARVAGERGHEVVVFEAANNPGGQIRLTAQSERRREMISIIDWRMRQCEKYDVTFHFNTWAEADTIEAENPDVVIIATGGL
PHTEVLTNGNELVVSSWDIISGDVKPGTNVLVFDDAGDHAGLQAAEFLANAGAKVEIMTPDRSFAPEVMAMNLVPYMRSL
QKYDVTFTVTYRLEAVEKSGNQLVALIGSDYGGIARQHSYDQVVINHGTIPLDDLYFELKPKSKNLGEVSYDQLLQGQPQ
SVIHNPEGKFQLFRIGDAVAARNTHAAVYDGLRIAKDI
>Mature_677_residues
SNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKERYRAYTVERAKGGVALTMTAGSAAVSRDSPPVFNNLLAYKDEI
VPWIREMTDAVHEQGAAIMIQLTHLGRRTRWDKGDWLPVVAPSHHREASHRAFPKKMEDWDIERIIKDFADAAERMKAGG
MDGIELEAYGHLIDQFASPLTNELGGPYGGALENRMRFCFDVFRAIRERVGNEFILGVRYTADECLPGGNGQAEGIEISK
RLRDSGLIDYLNVIRGHIDTDPGLTDVIPIQGMANSPHLDFAGEIRAATNFPTFHAAKIPDVATARHAIAAGKVDMVGMT
RAHMTDPHIVRKIIEKREEDIRPCVGANYCLDRIYQGGAAYCIHNAATGRELTMPHIVAKADVGKKVVIVGAGPAGLEAA
RVAGERGHEVVVFEAANNPGGQIRLTAQSERRREMISIIDWRMRQCEKYDVTFHFNTWAEADTIEAENPDVVIIATGGLP
HTEVLTNGNELVVSSWDIISGDVKPGTNVLVFDDAGDHAGLQAAEFLANAGAKVEIMTPDRSFAPEVMAMNLVPYMRSLQ
KYDVTFTVTYRLEAVEKSGNQLVALIGSDYGGIARQHSYDQVVINHGTIPLDDLYFELKPKSKNLGEVSYDQLLQGQPQS
VIHNPEGKFQLFRIGDAVAARNTHAAVYDGLRIAKDI

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=540, Percent_Identity=29.8148148148148, Blast_Score=219, Evalue=5e-58,
Organism=Escherichia coli, GI1787939, Length=388, Percent_Identity=25.7731958762887, Blast_Score=80, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=307, Percent_Identity=25.7328990228013, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17559802, Length=276, Percent_Identity=26.8115942028986, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17559804, Length=259, Percent_Identity=26.6409266409266, Blast_Score=75, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17564188, Length=264, Percent_Identity=25, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI72001454, Length=265, Percent_Identity=27.5471698113208, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17566914, Length=235, Percent_Identity=24.2553191489362, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6325086, Length=356, Percent_Identity=24.7191011235955, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321973, Length=364, Percent_Identity=23.0769230769231, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 74828; Mature: 74697

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKERYRAYTVERAKGGVALTMTAGS
CCCCCCCCCHHHHEEEEECEEEEECCCCCCCCCCCCHHHHHEEEEEECCCCEEEEEECCC
AAVSRDSPPVFNNLLAYKDEIVPWIREMTDAVHEQGAAIMIQLTHLGRRTRWDKGDWLPV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEE
VAPSHHREASHRAFPKKMEDWDIERIIKDFADAAERMKAGGMDGIELEAYGHLIDQFASP
ECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
LTNELGGPYGGALENRMRFCFDVFRAIRERVGNEFILGVRYTADECLPGGNGQAEGIEIS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHCCCCCCCCCCCHHHH
KRLRDSGLIDYLNVIRGHIDTDPGLTDVIPIQGMANSPHLDFAGEIRAATNFPTFHAAKI
HHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCEECCCC
PDVATARHAIAAGKVDMVGMTRAHMTDPHIVRKIIEKREEDIRPCVGANYCLDRIYQGGA
CCHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
AYCIHNAATGRELTMPHIVAKADVGKKVVIVGAGPAGLEAARVAGERGHEVVVFEAANNP
EEEEECCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCC
GGQIRLTAQSERRREMISIIDWRMRQCEKYDVTFHFNTWAEADTIEAENPDVVIIATGGL
CCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCC
PHTEVLTNGNELVVSSWDIISGDVKPGTNVLVFDDAGDHAGLQAAEFLANAGAKVEIMTP
CCHHEEECCCEEEEEECCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECC
DRSFAPEVMAMNLVPYMRSLQKYDVTFTVTYRLEAVEKSGNQLVALIGSDYGGIARQHSY
CCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCC
DQVVINHGTIPLDDLYFELKPKSKNLGEVSYDQLLQGQPQSVIHNPEGKFQLFRIGDAVA
CEEEEECCCCCHHHEEEEEECCCCCCCCCCHHHHHCCCCHHHEECCCCCEEEEEECCHHH
ARNTHAAVYDGLRIAKDI
CCCCCHHHHCCCHHHCCC
>Mature Secondary Structure 
SNDPLLQPYQLKHLTLRNRIIVTSHEPAYPEDGMPKERYRAYTVERAKGGVALTMTAGS
CCCCCCCCHHHHEEEEECEEEEECCCCCCCCCCCCHHHHHEEEEEECCCCEEEEEECCC
AAVSRDSPPVFNNLLAYKDEIVPWIREMTDAVHEQGAAIMIQLTHLGRRTRWDKGDWLPV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEE
VAPSHHREASHRAFPKKMEDWDIERIIKDFADAAERMKAGGMDGIELEAYGHLIDQFASP
ECCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
LTNELGGPYGGALENRMRFCFDVFRAIRERVGNEFILGVRYTADECLPGGNGQAEGIEIS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECHHHCCCCCCCCCCCHHHH
KRLRDSGLIDYLNVIRGHIDTDPGLTDVIPIQGMANSPHLDFAGEIRAATNFPTFHAAKI
HHHHHCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCCCCCEECCCC
PDVATARHAIAAGKVDMVGMTRAHMTDPHIVRKIIEKREEDIRPCVGANYCLDRIYQGGA
CCHHHHHHHHHCCCEEEEECCHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCC
AYCIHNAATGRELTMPHIVAKADVGKKVVIVGAGPAGLEAARVAGERGHEVVVFEAANNP
EEEEECCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCC
GGQIRLTAQSERRREMISIIDWRMRQCEKYDVTFHFNTWAEADTIEAENPDVVIIATGGL
CCEEEEEECHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCEEEEECCCC
PHTEVLTNGNELVVSSWDIISGDVKPGTNVLVFDDAGDHAGLQAAEFLANAGAKVEIMTP
CCHHEEECCCEEEEEECCEEECCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECC
DRSFAPEVMAMNLVPYMRSLQKYDVTFTVTYRLEAVEKSGNQLVALIGSDYGGIARQHSY
CCCCCCHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCCC
DQVVINHGTIPLDDLYFELKPKSKNLGEVSYDQLLQGQPQSVIHNPEGKFQLFRIGDAVA
CEEEEECCCCCHHHEEEEEECCCCCCCCCCHHHHHCCCCHHHEECCCCCEEEEEECCHHH
ARNTHAAVYDGLRIAKDI
CCCCCHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]