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The map label for this gene is adhCf

Identifier: 86361132

GI number: 86361132

Start: 454705

End: 455832

Strand: Reverse

Name: adhCf

Synonym: RHE_PF00402

Alternate gene names: 86361132

Gene position: 455832-454705 (Counterclockwise)

Preceding gene: 86361133

Following gene: 86361131

Centisome position: 70.94

GC content: 64.1

Gene sequence:

>1128_bases
ATGGACGTTCGCGCCGCAGTCGCCGTTCAGGCCGGAAAACCGCTCGAAGTGATGACCGTGCAGCTCGACGGCCCGAAGGC
CGGCGAAGTGCTGGTGGAGGTCAAGGCGACCGGCATCTGCCACACCGATGATTTCACCCTGTCGGGCGCCGATCCGGAAG
GCCTGTTCCCGGCCATCCTCGGCCATGAGGGCGCCGGCATTGTCGTCGACGTCGGCCCGGGTGTGACCTCGGTCAAGAAG
GGCGACCACGTCATTCCGCTTTACACGCCGGAATGCCGCGAGTGTTATTCCTGCACGTCCCGCAAGACCAATCTCTGCAC
CGCCATCCGCGCCACCCAGGGCCAGGGCGTGATGCCGGACGGCACCTCGCGCTTTTCGATCGGCAAGGACAAGATCCATC
ACTATATGGGCTGCTCGACCTTTGCCAATTACACGGTGCTGCCGGAGATCGCGCTCGCCAAGATCAATCCCGACGCGCCC
TTCGACAAGGTCTGCTACATCGGCTGCGGCGTCACCACAGGCATCGGCGCCGTCATCAACACCGCCAAGGTCGAGGTCGG
ATCGACGGCGATCGTCTTCGGCCTCGGCGGCATCGGCCTCAACGTCTTACAGGGTCTGCGGCTGGCCGGCGCCGACATGA
TCATCGGCGTCGATATCAACCCGGATCGCAAGGCCTGGGGCGAGAAGTTCGGCATGACGCATTTCGTCAATCCGAAGGAG
GTCGGCGACGACATCGTTCCCTATCTGGTCAACATGACCAAGCGGGGCGGCGACCTGATCGGCGGCGCCGACTACACCTT
CGACTGCACCGGCAATACCAAGGTGATGCGCCAGGCGCTGGAAGCCTCCCATCGCGGCTGGGGCAAATCGGTCATCATCG
GGGTTGCCGGCGCCGGCCAGGAAATCTCGACGCGGCCGTTCCAGCTGGTGACCGGCCGCAACTGGATGGGCACCGCCTTC
GGCGGCGCGCGCGGCCGCACCGACGTGCCGAAGATCGTCGACTGGTACATGCAGGGCAAGATCCAGATCGATCCGATGAT
CACCCACACCATGCCACTCGAAGACATCAACACGGGCTTCGAGCTGATGCACAAGGGCGAAAGCATCCGCGGCGTGGTGG
TGTACTGA

Upstream 100 bases:

>100_bases
CCGGGATGAACGAGATGAGGCCGAAGCAGATCGTTTCGATCTCGCGGTCGCATCCCGCATAGTTAACAACCGAATTTGAA
TTAACAGGAGGGATTATCAA

Downstream 100 bases:

>100_bases
GTCATGAAAACCATTGCGACCGACAAGTCTTACGGCGGCACTCAGGGCGTTTATGTCAATCGCTCCGAGGCCTGTGACTG
CGACATGACCTTCGCCGTGT

Product: formaldehyde dehydrogenase (glutathione)/alcohol dehydrogenase protein

Products: NA

Alternate protein names: Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH; S-(hydroxymethyl)glutathione dehydrogenase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MDVRAAVAVQAGKPLEVMTVQLDGPKAGEVLVEVKATGICHTDDFTLSGADPEGLFPAILGHEGAGIVVDVGPGVTSVKK
GDHVIPLYTPECRECYSCTSRKTNLCTAIRATQGQGVMPDGTSRFSIGKDKIHHYMGCSTFANYTVLPEIALAKINPDAP
FDKVCYIGCGVTTGIGAVINTAKVEVGSTAIVFGLGGIGLNVLQGLRLAGADMIIGVDINPDRKAWGEKFGMTHFVNPKE
VGDDIVPYLVNMTKRGGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSVIIGVAGAGQEISTRPFQLVTGRNWMGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLEDINTGFELMHKGESIRGVVVY

Sequences:

>Translated_375_residues
MDVRAAVAVQAGKPLEVMTVQLDGPKAGEVLVEVKATGICHTDDFTLSGADPEGLFPAILGHEGAGIVVDVGPGVTSVKK
GDHVIPLYTPECRECYSCTSRKTNLCTAIRATQGQGVMPDGTSRFSIGKDKIHHYMGCSTFANYTVLPEIALAKINPDAP
FDKVCYIGCGVTTGIGAVINTAKVEVGSTAIVFGLGGIGLNVLQGLRLAGADMIIGVDINPDRKAWGEKFGMTHFVNPKE
VGDDIVPYLVNMTKRGGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSVIIGVAGAGQEISTRPFQLVTGRNWMGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLEDINTGFELMHKGESIRGVVVY
>Mature_375_residues
MDVRAAVAVQAGKPLEVMTVQLDGPKAGEVLVEVKATGICHTDDFTLSGADPEGLFPAILGHEGAGIVVDVGPGVTSVKK
GDHVIPLYTPECRECYSCTSRKTNLCTAIRATQGQGVMPDGTSRFSIGKDKIHHYMGCSTFANYTVLPEIALAKINPDAP
FDKVCYIGCGVTTGIGAVINTAKVEVGSTAIVFGLGGIGLNVLQGLRLAGADMIIGVDINPDRKAWGEKFGMTHFVNPKE
VGDDIVPYLVNMTKRGGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSVIIGVAGAGQEISTRPFQLVTGRNWMGTAF
GGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLEDINTGFELMHKGESIRGVVVY

Specific function: Oxidizes long-chain aliphatic alcohols, long-chain hydroxylated fatty acids and S-hydroxymethylglutathione (hmGSH) in increasing order of preference. Shows little or no activity with short-chain aliphatic alcohols [H]

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI71565154, Length=373, Percent_Identity=57.372654155496, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI71565152, Length=380, Percent_Identity=43.421052631579, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI34577061, Length=375, Percent_Identity=44.5333333333333, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI4501929, Length=375, Percent_Identity=44.2666666666667, Blast_Score=322, Evalue=4e-88,
Organism=Homo sapiens, GI262073058, Length=376, Percent_Identity=43.3510638297872, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI71743840, Length=376, Percent_Identity=43.3510638297872, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI4501933, Length=375, Percent_Identity=43.4666666666667, Blast_Score=313, Evalue=1e-85,
Organism=Homo sapiens, GI156523966, Length=371, Percent_Identity=42.3180592991914, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI4501939, Length=364, Percent_Identity=42.5824175824176, Blast_Score=300, Evalue=1e-81,
Organism=Escherichia coli, GI1786552, Length=375, Percent_Identity=59.4666666666667, Blast_Score=433, Evalue=1e-123,
Organism=Escherichia coli, GI1787863, Length=363, Percent_Identity=23.6914600550964, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082125, Length=388, Percent_Identity=25.2577319587629, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1788073, Length=368, Percent_Identity=27.1739130434783, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI226510992, Length=369, Percent_Identity=25.7452574525745, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1786825, Length=382, Percent_Identity=25.6544502617801, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI87081918, Length=373, Percent_Identity=27.0777479892761, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25146526, Length=379, Percent_Identity=53.8258575197889, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71997431, Length=381, Percent_Identity=53.5433070866142, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17565904, Length=343, Percent_Identity=53.935860058309, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17562584, Length=378, Percent_Identity=26.1904761904762, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71988145, Length=375, Percent_Identity=25.8666666666667, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17562582, Length=382, Percent_Identity=25.3926701570681, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320033, Length=373, Percent_Identity=56.0321715817694, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319621, Length=377, Percent_Identity=28.3819628647215, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322619, Length=359, Percent_Identity=27.0194986072423, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6319955, Length=359, Percent_Identity=27.0194986072423, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6319257, Length=379, Percent_Identity=25.8575197889182, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319258, Length=280, Percent_Identity=27.8571428571429, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=372, Percent_Identity=26.0752688172043, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=367, Percent_Identity=25.8855585831063, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323729, Length=354, Percent_Identity=25.1412429378531, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737895, Length=374, Percent_Identity=59.3582887700535, Blast_Score=456, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.1; =1.1.1.284 [H]

Molecular weight: Translated: 39859; Mature: 39859

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRAAVAVQAGKPLEVMTVQLDGPKAGEVLVEVKATGICHTDDFTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEECEEEECCCEEECCCCCCCCCHHHH
GHEGAGIVVDVGPGVTSVKKGDHVIPLYTPECRECYSCTSRKTNLCTAIRATQGQGVMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEECCCCCCCCC
GTSRFSIGKDKIHHYMGCSTFANYTVLPEIALAKINPDAPFDKVCYIGCGVTTGIGAVIN
CCCEECCCHHHHHHHHCHHHHCCEEECCCEEEEEECCCCCCCCEEEEECCHHHHHHHHEE
TAKVEVGSTAIVFGLGGIGLNVLQGLRLAGADMIIGVDINPDRKAWGEKFGMTHFVNPKE
EEEEEECCEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCEECCCHHH
VGDDIVPYLVNMTKRGGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSVIIGVAGAGQ
HCCHHHHHHHHHHCCCCCEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
EISTRPFQLVTGRNWMGTAFGGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLEDINTGF
CCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHCCEEEECCEEEEECCHHHHCCCH
ELMHKGESIRGVVVY
HHHHCCCCCEEEEEC
>Mature Secondary Structure
MDVRAAVAVQAGKPLEVMTVQLDGPKAGEVLVEVKATGICHTDDFTLSGADPEGLFPAIL
CCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEECEEEECCCEEECCCCCCCCCHHHH
GHEGAGIVVDVGPGVTSVKKGDHVIPLYTPECRECYSCTSRKTNLCTAIRATQGQGVMPD
CCCCCEEEEECCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCEEEEEEECCCCCCCCC
GTSRFSIGKDKIHHYMGCSTFANYTVLPEIALAKINPDAPFDKVCYIGCGVTTGIGAVIN
CCCEECCCHHHHHHHHCHHHHCCEEECCCEEEEEECCCCCCCCEEEEECCHHHHHHHHEE
TAKVEVGSTAIVFGLGGIGLNVLQGLRLAGADMIIGVDINPDRKAWGEKFGMTHFVNPKE
EEEEEECCEEEEEEECCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCEECCCHHH
VGDDIVPYLVNMTKRGGDLIGGADYTFDCTGNTKVMRQALEASHRGWGKSVIIGVAGAGQ
HCCHHHHHHHHHHCCCCCEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEEECCCC
EISTRPFQLVTGRNWMGTAFGGARGRTDVPKIVDWYMQGKIQIDPMITHTMPLEDINTGF
CCCCCCEEEEECCCCCEECCCCCCCCCCCHHHHHHHHCCEEEECCEEEEECCHHHHCCCH
ELMHKGESIRGVVVY
HHHHCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8631716 [H]