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The map label for this gene is gfa [H]

Identifier: 86361130

GI number: 86361130

Start: 453235

End: 453807

Strand: Reverse

Name: gfa [H]

Synonym: RHE_PF00400

Alternate gene names: 86361130

Gene position: 453807-453235 (Counterclockwise)

Preceding gene: 86361131

Following gene: 86361129

Centisome position: 70.63

GC content: 61.61

Gene sequence:

>573_bases
ATGAGCAGCATAGCCATTCATCCGGCAGTGGATTCAGGCTTTCGAGCGACGGACGCTGCTTTCACAGGCGGGACACTCGT
GTGCAAATGCACGAGCAATCCCGTCAAGGTCAGGATCAAGGGCGATATCGCCCACAATCACGCCTGCGGCTGCACCAAGT
GCTGGAAGCCCGAGGGAGCAGTCTTTTCGGTCGTGGCCGTCGCTCCGACCGAGAGCGTCGAGGTGCTGGAGAACGGCGAC
AAGCTCGCCGTCGTCGATCCCGCTGCCTTGATCCAGCGGCACGCCTGCAAGCAATGCGGCGTGCATATGTATGGGCCGGT
CGAGCGCGAACATCCGTTCCAGGGATTGTCCTTCGTCCATCCGGAGCGCTTCCAGGAAAGCGGCTGGGCCAAGCCCGGCT
TTGCTGCTTTCGTCTCATCGATCATCGAAAGCGGCTTCGATCCCGCCAAGATGGACGCGGTCAGGGCGCAGTTGAAGGCT
TCGGGCCTCGAGCCTTATGATTGCCTGTCGCCCGGCCTGATGGACTATATCGCGACCTGGACTGCCAAGAAGAGCGGTGT
GCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGACGATCATCTGCGCTGGCACGCGCAGAGGTTGGAGAACTGATTTTAGCGAGCTCGGCCGGGCATAACGCAAACGGCCA
GGGAGGAAGGGAGAAAAGCA

Downstream 100 bases:

>100_bases
ATTGTCAGGGGAGCGCCGACGGCGGCGCTCCCCCATCAAGCCGGGCATTGGATGATAGACGCTCCATGACGCCCCTCCGC
CGCTTTGTCGGCGCTTCCGA

Product: glutathione-dependent formaldehyde-activating enzyme

Products: NA

Alternate protein names: S-(hydroxymethyl)glutathione synthase [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSSIAIHPAVDSGFRATDAAFTGGTLVCKCTSNPVKVRIKGDIAHNHACGCTKCWKPEGAVFSVVAVAPTESVEVLENGD
KLAVVDPAALIQRHACKQCGVHMYGPVEREHPFQGLSFVHPERFQESGWAKPGFAAFVSSIIESGFDPAKMDAVRAQLKA
SGLEPYDCLSPGLMDYIATWTAKKSGVLAA

Sequences:

>Translated_190_residues
MSSIAIHPAVDSGFRATDAAFTGGTLVCKCTSNPVKVRIKGDIAHNHACGCTKCWKPEGAVFSVVAVAPTESVEVLENGD
KLAVVDPAALIQRHACKQCGVHMYGPVEREHPFQGLSFVHPERFQESGWAKPGFAAFVSSIIESGFDPAKMDAVRAQLKA
SGLEPYDCLSPGLMDYIATWTAKKSGVLAA
>Mature_189_residues
SSIAIHPAVDSGFRATDAAFTGGTLVCKCTSNPVKVRIKGDIAHNHACGCTKCWKPEGAVFSVVAVAPTESVEVLENGDK
LAVVDPAALIQRHACKQCGVHMYGPVEREHPFQGLSFVHPERFQESGWAKPGFAAFVSSIIESGFDPAKMDAVRAQLKAS
GLEPYDCLSPGLMDYIATWTAKKSGVLAA

Specific function: Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfa family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014185
- InterPro:   IPR006913
- InterPro:   IPR011057 [H]

Pfam domain/function: PF04828 GFA [H]

EC number: =4.4.1.22 [H]

Molecular weight: Translated: 20190; Mature: 20059

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIAIHPAVDSGFRATDAAFTGGTLVCKCTSNPVKVRIKGDIAHNHACGCTKCWKPEGA
CCCEEECCCCCCCCCCCCCEECCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
VFSVVAVAPTESVEVLENGDKLAVVDPAALIQRHACKQCGVHMYGPVEREHPFQGLSFVH
EEEEEEECCCCCHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PERFQESGWAKPGFAAFVSSIIESGFDPAKMDAVRAQLKASGLEPYDCLSPGLMDYIATW
HHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHH
TAKKSGVLAA
CCCCCCCCCC
>Mature Secondary Structure 
SSIAIHPAVDSGFRATDAAFTGGTLVCKCTSNPVKVRIKGDIAHNHACGCTKCWKPEGA
CCEEECCCCCCCCCCCCCEECCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
VFSVVAVAPTESVEVLENGDKLAVVDPAALIQRHACKQCGVHMYGPVEREHPFQGLSFVH
EEEEEEECCCCCHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
PERFQESGWAKPGFAAFVSSIIESGFDPAKMDAVRAQLKASGLEPYDCLSPGLMDYIATW
HHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHH
TAKKSGVLAA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]